Sperm motility, fertilization, and larval development of silver catfish (Rhamdia quelen) in copper-contaminated water

The objective of this study was to evaluate the effect of copper-contaminated water on sperm motility, fertilization, and embryonic and larval development of silver catfish (Rhamdia quelen). A randomized experimental design with five treatments and four replicates was used. Two experiments were carried out: (1) controlled fertilization was performed under different levels of copper contamination and egg hatching was performed in clean water; and (2) copper-contaminated water was used for both fertilization and hatching assays. The time of sperm motility and sperm motility rates linearly decreased with increasing copper concentration in the water. Fertilization and hatching rates were also affected when the concentrations of copper in the water were above 0.0979 mg Cu+2 L-1 and 0.0331 mg Cu+2 L-1, respectively. Gamete exposure to levels between 15 mg Cu+2 L-1 and 60 mg Cu+2 L-1 for short periods of time negatively affected sperm motility, oocyte fertilization, and egg hatching rates. In addition, when gametes and embryos were exposed at levels above 0.03 mg Cu+2 L-1 during long periods of time, egg hatching rates were reduced, and at levels between 0.05 mg Cu+2 L-1 and 0.20 mg Cu+2 L-1 the number of abnormal larvae increased.

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Garlic essential oil increases rates of eggs fertilization and hatching of Rhamdia quelen larvae in an artificial incubation system

Ciência Rural ◽

10.1590/0103-8478cr20160803 ◽

2017 ◽

Vol 47 (6) ◽

Author(s):

Nilton Garcia Marengoni ◽

Letícia Angélica Pereira ◽

Luciane Aline Weiss ◽

Mateus Antonio Besen ◽

Robie Allan Bombardelli

Keyword(s):

Essential Oil ◽

Normal Development ◽

Linear Effect ◽

Silver Catfish ◽

Rhamdia Quelen ◽

Larval Hatching ◽

Randomized Experimental Design ◽

Water Recirculation ◽

Stereo Microscope ◽

Garlic Essential Oil

ABSTRACT: The objective of this study was to evaluate the effects of garlic (Allium sativum) essential oil on the rates of eggs fertilization and hatching and on the normal development of larvae of artificially incubated silver catfish (Rhamdia quelen). The experiment was carried out using a completely randomized experimental design with four treatments and five replicates. The treatments consisted of introducing garlic essential oil into the incubators, which used a closed system of water recirculation, at concentrations of 0, 1, 3, and 5mg L-1. Rates of eggs fertilization and larval hatching were determined at 10 and 24h after the beginning of eggs hydration. Morphology of larvae was assessed with the aid of a stereo microscope to determine the rate of normal development. The concentration of 5mg L-1 promoted better rates of eggs fertilization and larval hatching. Concentrations of garlic essential oil had a linear effect on eggs fertilization rates and larval hatching. Garlic oil did not influence the morphology of the larvae (P>0.05). It is recommended to use 5mg L-1 of garlic essential oil to promote better rates of eggs fertilization and larval hatching of artificially incubated silver catfish.

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Stable isotope labelling of Ceratitis capitata

Plant Protection Science ◽

10.17221/13/2018-pps ◽

2018 ◽

Vol 55 (No. 1) ◽

pp. 54-60 ◽

Cited By ~ 1

Author(s):

Hasan Al-Khshemawee ◽

Manjree Agarwal ◽

Yonglin Ren

Keyword(s):

Stable Isotopes ◽

Larval Development ◽

Ceratitis Capitata ◽

Mating Behaviour ◽

Fruit Fly ◽

Adult Survival ◽

Stable Isotope Labelling ◽

Egg Hatching ◽

Sugar Water ◽

Hatching Rates

The use of stable isotopes to label an insect species, the Mediterranean fruit fly Ceratitis capitata (Diptera: Tephidae) (medfly) was investigated. Labelling allows mating and life history characteristics to be investigated experimentally. <sup>13</sup>C<sub>6</sub>-glucose was incorporated into the diet of medflies at various stages of development by adding it to larval media or providing adults with sugar water. Data was collected from egg hatching until the death of adults. The results show that stable isotopes successfully labelled medflies in laboratory conditions. There were significant differences between labelled and unlabelled treatments in terms of eggs hatching rates, larval development, pupae emergence, adult survival, and mating behaviour. Labelling during larval development, and combined labelling at the larval and adult stages, resulted in detectable values. Labelling in the larval stage had no effect on mating behaviour, but that in the adult stage did. This study demonstrates that it is possible to label adult medflies and to detect the label after mating.

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Salinity on artificial reproduction of silver catfish (Rhamdia quelen)

Ciência Rural ◽

10.1590/0103-8478cr20131337 ◽

2015 ◽

Vol 45 (3) ◽

pp. 458-463 ◽

Cited By ~ 1

Author(s):

Gabriel Bernardes Martins ◽

Sérgio Renato Noguez Piedras ◽

Juvêncio Luís Osório Fernandes Pouey ◽

Denise Calisto Bongalhardo ◽

Ricardo Berteaux Robaldo

Keyword(s):

Sperm Motility ◽

Artificial Reproduction ◽

Silver Catfish ◽

Rhamdia Quelen ◽

Reproduction Performance

Attempting to improve reproduction performance and ichthyo prophylaxis, this study evaluated the effects of maintaining silver catfish (Rhamdia quelen) broodstock in different saline concentrations (0, 2, 4, 6 and 8‰) on gametes quality and reproductive viability. The results showed that sperm percent motility did not change between 0 and 4‰, but it was reduced at 6‰, and sperm became immotile at 8‰ salinity. Sperm motility time was increased (almost five fold) at 6‰. Salinities up to 4‰ prevented fertilization and hatching, proving their deleterious effects on oocytes and embryos. Therefore, media up to 4‰ salinity may be an alternative for icthyo prophylaxis, although fertilization and incubation must be done in freshwater medium

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Incubation Temperature Influences the Duration of Egg Hatching and Early Development in the Boreal Digging Frog, Kaloula Borealis

Journal of Zoo Biology ◽

10.33687/zoobiol.003.01.2267 ◽

2020 ◽

Vol 3 (1) ◽

pp. ACCEPTED

Author(s):

Rho-Jeong Rae

Keyword(s):

Water Temperature ◽

Early Life ◽

Rainy Season ◽

Incubation Temperature ◽

Life Stages ◽

Early Life Stages ◽

Egg Hatching ◽

Significant Difference ◽

The Mean ◽

Hatching Rates

This study investigated the boreal digging frog, Kaloula borealis, to determine the egg hatching period and whether the hatching period is affected by incubation temperature. The results of this study showed that all the eggs hatched within 48 h after spawning, with 28.1% (±10.8, n=52) hatching within 24 h and 99.9% (±0.23, n=49) within 48 h after spawning. A significant difference was noted in the mean hatching proportion of tadpoles at different water temperatures. The mean hatching rates between 15 and 24 h after spawning was higher at a water temperature of 21.1 (±0.2) °C than at 24.1 (±0.2) °C. These results suggest that incubation temperature affected the early life stages of the boreal digging frog, since they spawn in ponds or puddles that form during the rainy season.

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Addition of tea tree oil (Melaleuca alternifolia) in diet minimize biochemical disturbances in silver catfish Rhamdia quelen exposed to the antiparasitic amitraz

Aquaculture ◽

10.1016/j.aquaculture.2021.736954 ◽

2021 ◽

pp. 736954

Author(s):

Tamires R. dos Reis ◽

Matheus D. Baldissera ◽

Carine F. Souza ◽

Bernardo Baldisserotto ◽

Julia Corá Segat ◽

...

Keyword(s):

Tea Tree Oil ◽

Melaleuca Alternifolia ◽

Tea Tree ◽

Silver Catfish ◽

Rhamdia Quelen

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Tea tree oil attenuates cerebral damage in silver catfish (Rhamdia quelen) fed with an aflatoxin-contaminated diet

Aquaculture ◽

10.1016/j.aquaculture.2020.735223 ◽

2020 ◽

Vol 523 ◽

pp. 735223 ◽

Cited By ~ 1

Author(s):

Carine de Freitas Souza ◽

Matheus Dellaméa Baldissera ◽

Sharine Descovi ◽

Pedro René Eslava-Mocha ◽

Carla C. Zeppenfeld ◽

...

Keyword(s):

Cerebral Damage ◽

Tea Tree Oil ◽

Tea Tree ◽

Silver Catfish ◽

Rhamdia Quelen

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Eggs viability of Aedes aegypti Linnaeus (Diptera, Culicidae) under different environmental and storage conditions in Manaus, Amazonas, Brazil

Brazilian Journal of Biology ◽

10.1590/1519-6984.19815 ◽

2016 ◽

Vol 77 (2) ◽

pp. 396-401 ◽

Cited By ~ 14

Author(s):

V. C. Soares-Pinheiro ◽

W. Dasso-Pinheiro ◽

J. M. Trindade-Bezerra ◽

W. P. Tadei

Keyword(s):

Aedes Aegypti ◽

Storage Conditions ◽

Amazon Region ◽

Storage Site ◽

Egg Hatching ◽

Plastic Bag ◽

The Mean ◽

And Storage ◽

Storage Type ◽

Hatching Rates

Abstract The viability of Aedes aegypti eggs was assessed in the Amazon region. The eggs were maintained under different conditions: indoors (insectarium) and outdoors (natural environment), as well as in different storage types (plastic cup, paper envelope, plastic bag) for different days. Egg viability was measured as the mean of hatchings observed from egg-bearing sheets of filter paper immersed in water, using three sheets randomly selected from each storage type and at both sites. There were significant differences in the viability of Ae. aegypti eggs with respect to the location (F=30.40; DF=1; P<0.0001), storage type (F=17.66; DF=2; P<0.0001), and time of storage (F=49.56; DF=9; P<0.0001). The interaction between storage site versus storage type was also significant (F=15.96; DF=2; P<0.0001). A higher hatching mean was observed for the eggs kept in the insectarium than for those outdoors (32.38 versus 7.46). Hatching rates of egg batches stored for 12 to 61 days ranged between 84 and 90%. A reduction was observed between 89 and 118 days, with values of 63 and 48%, respectively. With respect to type of storage, mean egg hatching was higher for the eggs in plastic cups (44.46). It was concluded that the viability of the eggs of Ae. aegypti in the Amazon region remains high up to 4 months, after which it declines drastically, although in this study hatching occurred for up to 8 months in very low percentages.

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