ISOLASI BAKTERI SELULOLITIK PADA KONSURSIOM BAKTERI PEREDUKSI SULFAT DENGAN PENAMBAHAN SERBUK GERGAJI KAYU AKASIA

2020 ◽  
Vol 9 (2) ◽  
pp. 82-91
Author(s):  
Ni'matul Murtafia'ah

Latar Belakang: Pencemaran lingkungan menjadi salah satu masalah bagi kesehatan manusia. Pencemaran lingkungan dapat menurunkan pH yang dapat mempengaruhi proses fisiologi dan molekuler dengan menonaktifkan enzim, menggantikan unsur penting sehingga mengganggu integritas membran. Penurunan pH mengakibatkan peningkatan kandungan sulfat pada perairan sehingga mempengaruhi kestabilan ekosistem.Tujuan: untuk mengetahui karakter bakteri selulolitik pada konsorsium Bakteri Pereduksi Sulfat dalam mereduksi sulfat pada skala batch cultureMetode: : Penelitian dilaksanakan dengan metode bioremidiasi menggunakan Bakteri Pereduksi Sulfat (BPS) skala batch culture menggunakan bioreaktor menggunakan media Carboxymethyl cellulose (CMC). Konsorsium yang digunakan yaitu konsorsium yang efektif mereduksi logam Mn. Metode analisis data menggunakan Rancangan Acak Lengkap (RAL), perlakuan dengan pemberian zeolit sebesar 20 gr/L sedangkan kadar serbuk gergaji sebesar 0%;1,25%; 2,5% dan 5%.Hasil: Hasil inokulasi bakteri selulolitik melalui konsorsium BPS pada medium Postgate B yang di inokulasikan pada medium CMC yang paling optimal yaitu pada perlakuan Zc3KK1 pada pengenceran 10-2 dengan kode isolat FKK1 luas diameter zona bening 26,67mm dengan diameter koloni 5 mm.Kesimpulan: Bakteri dapat menghidrolisis selulosa baik secara aerob maupun anaerob yang ditunjukan terbentuknya zona bening dengan diameter maksimum sebesar 26,67 mm dengan penambahan serbuk gergaji 2,5%. Besar kecilnya ukuran zona bening dipengaruhi oleh aktivitas spesifik enzim di daerah amorf pada substrat menyebabkan CMC terhidrolisis secara efisien.

TAPPI Journal ◽  
2009 ◽  
Vol 8 (6) ◽  
pp. 29-35 ◽  
Author(s):  
PEDRAM FATEHI ◽  
LIYING QIAN ◽  
RATTANA KITITERAKUN ◽  
THIRASAK RIRKSOMBOON ◽  
HUINING XIAO

The application of an oppositely charged dual polymer system is a promising approach to enhance paper strength. In this work, modified chitosan (MCN), a cationic polymer, and carboxymethyl cellulose (CMC), an anionic polymer, were used sequentially to improve paper strength. The adsorption of MCN on cellulose fibers was analyzed via polyelectrolyte titration. The formation of MCN/CMC complex in water and the deposition of this complex on silicon wafers were investigated by means of atomic force microscope and quasi-elastic light scattering techniques. The results showed that paper strength was enhanced slightly with a layer-by-layer assembly of the polymers. However, if the washing stage, which was required for layer-by-layer assembly, was eliminated, the MCN/CMC complex was deposited on fibers more efficiently, and the paper strength was improved more significantly. The significant improvement was attributed to the extra development of fiber bonding, confirmed further by scanning electron microscope observation of the bonding area of fibers treated with or without washing. However, the brightness of papers was somewhat decreased by the deposition of the complex on fibers. Higher paper strength also was achieved using rapid drying rather than air drying.


2009 ◽  
Vol 40 (2) ◽  
pp. 19-26 ◽  
Author(s):  
Soojung LEE ◽  
Yasutsugu MIWA ◽  
Ryohei NISHIMURA ◽  
Ung-il CHUNG ◽  
Shigeki SUZUKI ◽  
...  

2020 ◽  
Vol 23 (03) ◽  
pp. 33-49
Author(s):  
Ni’matul Mauludiyah ◽  
Devi Ayu Aprillia ◽  
Viddy Agustian Rosyidi ◽  
Lusia Oktora Ruma Kumala Sari

2000 ◽  
Vol 42 (12) ◽  
pp. 149-154 ◽  
Author(s):  
M. Okada ◽  
H. Morinaga ◽  
W. Nishijima

Effects of PAC on bacterial activity were evaluated by sequencing batch cultures (20 hours each) of E.coli K-12 on synthetic medium containing glucose as a sole carbon source. Four suspended sequencing batch culture systems were operated; CP: cultures supplemented with PAC, CR: cultures with removal of metabolites by PAC at the end of each batch culture, CD: cultures supplemented with PAC in dialysis tubing to separate from E.coli, and CC: cultures without PAC (control). The supernatant of each batch culture was filtered through a membrane filter (0.2 μm) and was mixed with the same volume of fresh medium to be used as the medium for the next batch culture. The sequencing batch cultures were repeated three times for all the systems. The bacterial growth in CC was inhibited with the increase in the number of batch cultures. Although a significant amount of metabolites was accumulated in the 3rd batch culture of CC, little accumulation was noted in the 3rd batch culture of CP. No growth inhibition was noted in CP for all the batch cultures. The little differences in the bacterial yield and metabolite accumulation between CR and CD suggested that adsorption/desorption of metabolites with PAC did not play a major role in bacterial growth. PAC addition may partly stimulate the growth by the removal of growth inhibiting metabolites. However, the fact that CP showed higher yield than CR and CD indicated that the contact between bacteria and PAC plays a significant role in the growth of bacteria.


Polymers ◽  
2018 ◽  
Vol 10 (6) ◽  
pp. 605 ◽  
Author(s):  
Jungmin Lee ◽  
Soohee Park ◽  
Hyun-gyoo Roh ◽  
Seungtaek Oh ◽  
Sunghoon Kim ◽  
...  

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