Analysis of Purity and Concentration of Isolated DNA in Making Raw DNA of Rat Species

2021 ◽  
Vol 1 (2) ◽  
pp. 1-5
Author(s):  
Alfi Sophian ◽  
Andi Syukur
Keyword(s):  
Dna Analysis ◽  
Rna Extraction ◽  
Average Concentration ◽  
Raw Material ◽  
Analysis Method ◽  
Dna Testing ◽  
Target Species ◽  
Average Value ◽  
Dna Extraction Method ◽  
Synthetic Materials

Analysis of the purity and concentration of isolated DNA in the manufacture of standard rat DNA was carried out to see whether the isolation carried out could produce good quality DNA. The purpose of this study is to provide information on the manufacture of raw DNA in species DNA testing where the raw material that has been purchased so far made from synthetic materials can be more economical if using DNA material derived from the meat raw material of the target species. The DNA extraction method used is the column spin method or column centrifuge using the Intron Patho Gene-Spin (Viral DNA/RNA) extraction kit. Analysis method of concentration and purity of isolated DNA was analyzed based on the average value of concentration and purity which was read using a nanophotometer. Based on the results of the research conducted, the results of the isolated DNA concentration values ​​were in the concentration range of 41,250 ng/ mL to 42,300 ng/mL, with the average concentration of isolated DNA was 41,777 ng/mL. For the value of the purity of the isolated DNA whose absorbance was read using a nanophotometer at a wavelength of A260/A280, the results were between 2,301 to 2,384 with the average value of purity being at 2,326. This study concludes that all the extracted samples that showed the results of the DNA analysis produced were included in the good DNA category.

scholarly journals Qualitative and Quantitative Characterization of Monosaccharide Components of Salvia miltiorrhiza, Liguspyragine Hydrochloride, and Glucose Injection

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Fa-huan Ge ◽  
Xian-peng Ma ◽  
Jin-fang Ma ◽  
Chang-qiong Bi ◽  
Tian-ling Chen ◽  
...  
Keyword(s):  
Salvia Miltiorrhiza ◽  
Gradient Elution ◽  
Average Concentration ◽  
Raw Material ◽  
Precolumn Derivatization ◽  
Quantitative Characterization ◽  
Average Value ◽  
Glucose Injection ◽  
Phosphate Buffered Saline

Salvia miltiorrhiza, liguspyragine hydrochloride, and glucose injection (SLGI) was made of Salvia miltiorrhiza Bge., liguspyragine hydrochloride, glucose, and glycerin. There were many kinds of monosaccharide components in SLGI, which might be from the raw material and Salvia miltiorrhiza Bge. Separation was performed on a Phenomenex Luna C18 analytical column (250 mm × 4.6 mm i.d., 5 μm, AccuStandard Inc., USA) at 30°C. The mobile phase consisted of two solvents: 0.1 mol/L phosphate-buffered saline (pH 6.7) (solvent A) and acetonitrile (solvent B) with gradient elution. The flow rate was maintained at 1.0 mL/min. Five kinds of monosaccharide components, glucose, D-mannose, L-rhamnose monohydrate, galactose, and xylose, were detected by precolumn derivatization HPLC, and their contents were compared with each other. And finally, concentrations of glucose in SLGI were determined and they were higher than the values of marked amount, which showed that one source of glucose might be from Salvia miltiorrhiza Bge. in SLGI. The average concentration of glucose was 5.18 g/100 mL, which was near the average value at 5.25 g/100 mL detected by ultraviolet spectrophotometry and also close to the marked amount (5.00 g/100 mL) on the label.

Judgments of Value, Judgments of Fact: The Ethical Dimension of Biohistorical Research

2006 ◽  
Vol 28 (1) ◽  
pp. 93-99
Author(s):  
Russell Lewis
Keyword(s):  
Dna Analysis ◽  
Ethical Framework ◽  
Ethical Dimension ◽  
Dna Testing ◽  
Ethical Guidelines ◽  
Value Judgments ◽  
Scientific Methods ◽  
The Public ◽  
Historical Figures ◽  
Scientific Analysis

The public increasingly views DNA testing as an unassailable way to verify the identity of historical figures. The Chicago Historical Society explored the appropriateness of DNA analysis and other forensic scientific methods to authenticate Lincoln assassination-related artifacts in its collection. The study concluded that DNA testing would damage or destroy the artifacts. More importantly, it determined that DNA and other scientific analysis of historical artifacts or historical figures' remains should be done only in the context of an ethical framework. The article discusses the development of ethical guidelines for museums and historians to follow when considering such studies.

Anisotropy Gas Reservoir Drainage Radius and Quantitative Well Interference Study

2013 ◽  
Vol 275-277 ◽  
pp. 1285-1291 ◽  
Author(s):  
Zheng Long Gao ◽  
Hong Fu Fan ◽  
Zhi Bin Gao
Keyword(s):  
Production Rate ◽  
Major Axis ◽  
Productivity Analysis ◽  
Analysis Method ◽  
Equivalent Radius ◽  
Average Value ◽  
Drainage Radius ◽  
Well Spacing ◽  
Cumulative Production ◽  
The Difference

Unstable productivity analysis method was used to obtain the equivalent radius of 77 wells and the result shows that the equivalent radius ranges from 30 to 970m with an average value of 230m in McKittrick Hills. The difference range of the radius is mainly caused by varying formation properties, gas saturation, production time, etc. Permeability anisotropy changes the drainage from round to ellipse. The major axis and the minor axis of the ellipse are determined by the ratio of major and minor permeability. Current pressure distribution was obtained and was found to be consistent with the modified drainage results, which demonstrates that the unstable productivity analysis method is applicable in the study of gas well drainage radius. An interference well and an observation well’s model was constructed to study well interference quantitatively. When the well spacing is larger than 750m, the productivity will be reduced by 20%. The production rate of interference well is more sensitive to the cumulative production of observation well, when the production rate of interference well is below 16.8×104m3/d.

scholarly journals Determination of the melting temperature, heat of fusion, and purity analysis of different samples of zidovudine (AZT) using DSC

2010 ◽  
Vol 46 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Adriano Antunes Souza Araújo ◽  
Marília dos Santos Bezerra ◽  
Sílvia Storpirtis ◽  
Jivaldo do Rosário Matos
Keyword(s):  
The United States ◽  
Raw Material ◽  
Heat Of Fusion ◽  
Average Value ◽  
Purity Analysis ◽  
Temperature Heat ◽  
Chemical Purity ◽  
Dsc Curves ◽  
States Pharmacopeia

The determination of chemical purity, melting range, and variation of enthalpy in the process of characterizing medicines is one of the principal requirements evaluated in quality control of the pharmaceutical industry. In this study, the method of purity determination using DSC was outlined, as well as the application of this technique for the evaluation of commercial samples of zidovudine (AZT) (raw material) supplied by different laboratories. To this end, samples from six different laboratories (A, B, C, D, E, and F) and the standard reference (R) from the United States Pharmacopeia (USP) were analyzed. The DSC curves were obtained in the temperature range of 25 to 200 ºC under the dynamic atmosphere of N2 (50 mL min-1), heating rate of β=2 ºC min-1, using an Al capsule containing approximately 2 mg of sample material. The results demonstrated that the standard reference presented a proportion of 99.83% whereas the AZT samples presented a variation ranging from 97.59 to 99.54%. In addition, the standard reference was found to present a temperature of onset of melting point of 122.80 °C. Regarding the samples of active agents provided by the different laboratories, a variation ranging from 118.70 to 122.87 °C was measured. In terms of ΔHm, the samples presented an average value of 31.12 kJ mol-1.

scholarly journals Development and Application of DNA Analysis Method for Identificaion of Main Ingredients in Starch

2013 ◽  
Vol 28 (2) ◽  
pp. 181-187
Author(s):  
Yong-Chjun Park ◽  
Mi-Ra Kim ◽  
Yong-Sang Kim ◽  
Ho-Yeon Lee ◽  
Kyu-Heon Kim ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Analysis Method

scholarly journals PENGENDALIAN PERSEDIAAN BAHAN BAKU DALAM PROSES PRODUKSI DENGAN MENGGUNAKAN METODE ECONOMIC ORDER QUANTITY (EOQ)

2019 ◽  
Vol 1 (2) ◽  
pp. 415-423
Author(s):  
Elia Rahayu R ◽  
Nor Norisanti ◽  
Acep Samsudin
Keyword(s):  
Raw Materials ◽  
Raw Material ◽  
Economic Order Quantity ◽  
Economic Order ◽  
Order Quantity ◽  
Analysis Method ◽  
Inventory Cost ◽  
Total Inventory ◽  
The Difference ◽  
Quantitative Descriptive

The purpose of this study is to control the supply of raw materials using the Economic Order Quantity (EOQ) method in Tahu Nugraha Jaya Sukabumi UKM. The data analysis method used is quantitative descriptive to describe and describe the data to be examined and then processed using EOQ. This study uses the EOQ method to determine the total inventory cost. The data needed in this study are the number of purchases of raw materials, the amount of use of raw materials, storage costs, and ordering costs. The results of this study indicate that by applying the EOQ method can further optimize the supply of raw materials by minimizing raw materials with increased inventory. With the application of the Economic Order Quantity (EOQ) method it shows more efficient than conventional methods of the company. Conclusions, seen from the difference in the TIC of the two methods, the more efficient method is the Economic Order Quantity (EOQ) method that is equal to 244,392.94 while the calculation used by the company is 374,325. so that it can be obtained that there is a difference between the Company TIC and the EIC method TIC. Keywords: Raw Material Inventory, Production Process

scholarly journals Species composition of glass eels (Anguilla spp.) recruiting to the Palu River, Central Sulawesi

2018 ◽  
Author(s):  
Novalina Serdiati ◽  
Samliok Ndobe ◽  
Abigail Moore ◽  
Deddy Wahyudi
Keyword(s):  
Species Composition ◽  
Dna Analysis ◽  
Temporal Patterns ◽  
Clove Oil ◽  
Analysis Method ◽  
Substantial Variation ◽  
Anguilla Marmorata ◽  
Glass Eels ◽  
Central Sulawesi ◽  
Tropical Eel

Demand for tropical eel seed has been increased and many tropical eel populations are under pressure. To conserve eel biodiversity and manage eel populations sustainably, it is necessary to identify eel species and their recruitment patterns at regional and watershed scales. The research objective was to determine the species composition and temporal recruit-ment patterns of glass eels recruiting to Palu River in Central Sulawesi. Glass eels sampling were conducted in January-April 2009, May-November 2010 and April-December 2011. Identification under anaesthetic (15-17.5 ppm clove oil solution) was based mainly on the number of ano-dorsal vertebrae (ADV). Species composition was dominated by two commercially species, Anguilla marmorata and A. bicolor pacifica with substantial variation and no clear temporal patterns. Specimens of other species that important from conservation and biodiversity aspects were present at each month but cannot be accurately identified using the ADV method. DNA analysis method is required to identify these specimens.

Panel Comment: The Attempt to Pass the Genetic Privacy Act in Maryland

1995 ◽  
Vol 23 (4) ◽  
pp. 367-370 ◽  
Author(s):  
Neil A. Holtzman
Keyword(s):  
Informed Consent ◽  
Genetic Analysis ◽  
Dna Analysis ◽  
Dna Testing ◽  
Genetic Privacy ◽  
Privacy Act ◽  
Judicial Proceedings ◽  
Exclusive Property ◽  
Privacy Legislation ◽  
Unauthorized Disclosure

The Genetic Privacy Act (GPA) is a comprehensive effort to protect individuals from unauthorized analysis of their DNA and from unauthorized disclosure of information resulting from genetic analysis. Irrespective of merit, every bill must survive legislative scrutiny. This is a considerable challenge, particularly for a bill as complex and far-reaching as the GPA. To illustrate my point, I describe the fate of two bills introduced into the Maryland Senate in 1995 by Senator Jennie Forehand. The first, also entitled the Genetic Privacy Act (S. 645), was a slightly modified version of the model legislation prepared by Annas, Glantz, and Roche. After a hearing, the bill received a 9-2 unfavorable vote from the Economic and Environmental Affairs Committee. The second was a much shorter bill, DNA Testing – Informed Consent and Confidentiality (S. 707), which simply stated that “DNA analysis may only be performed with the informed consent of the person being analyzed” and that the results of such analysis “are the exclusive property of the person tested, are confidential, and may not be disclosed without the consent of the person being tested.” This bill had a hearing but was never put to a vote by the Judicial Proceedings Committee. My principal aim is to examine the testimony on these bills. I will conclude with some suggestions about accomplishing the goals of genetic privacy legislation.

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