scholarly journals Qualitative and Quantitative Characterization of Monosaccharide Components of Salvia miltiorrhiza, Liguspyragine Hydrochloride, and Glucose Injection

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Fa-huan Ge ◽  
Xian-peng Ma ◽  
Jin-fang Ma ◽  
Chang-qiong Bi ◽  
Tian-ling Chen ◽  
...  

Salvia miltiorrhiza, liguspyragine hydrochloride, and glucose injection (SLGI) was made of Salvia miltiorrhiza Bge., liguspyragine hydrochloride, glucose, and glycerin. There were many kinds of monosaccharide components in SLGI, which might be from the raw material and Salvia miltiorrhiza Bge. Separation was performed on a Phenomenex Luna C18 analytical column (250 mm × 4.6 mm i.d., 5 μm, AccuStandard Inc., USA) at 30°C. The mobile phase consisted of two solvents: 0.1 mol/L phosphate-buffered saline (pH 6.7) (solvent A) and acetonitrile (solvent B) with gradient elution. The flow rate was maintained at 1.0 mL/min. Five kinds of monosaccharide components, glucose, D-mannose, L-rhamnose monohydrate, galactose, and xylose, were detected by precolumn derivatization HPLC, and their contents were compared with each other. And finally, concentrations of glucose in SLGI were determined and they were higher than the values of marked amount, which showed that one source of glucose might be from Salvia miltiorrhiza Bge. in SLGI. The average concentration of glucose was 5.18 g/100 mL, which was near the average value at 5.25 g/100 mL detected by ultraviolet spectrophotometry and also close to the marked amount (5.00 g/100 mL) on the label.

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Fengjuan Dong ◽  
Na Liu ◽  
Zhen Sun ◽  
Xiaolong Wei ◽  
Haonan Wang ◽  
...  

The complex pore structure of low-permeability sandstone reservoir makes it difficult to characterize the heterogeneity of pore throat. Taking the reservoir of Sanjianfang formation in QL oilfield as an example, the fractal dimension of different storage spaces is calculated by using fractal theory based on casting thin section, scanning electron microscope, and high-pressure mercury injection, and the correlation between porosity, permeability, and contribution of different storage space permeabilities is analyzed. The results show that the reservoir of Sanjianfang formation in QL oilfield mainly develops small pores, fine pores, and micropores, and the fractal dimension of micropore structure is between 2.6044 and 2.9982, with an average value of 2.8316. The more complex the pore structure is, the stronger the microheterogeneity is. The higher the fractal dimension, the more complex the pore structure and the smaller the porosity and permeability. The fractal dimensions of small pores, fine pores, and micropores increase successively with the decrease in pore radius, and the microstructure heterogeneity of large pores is weaker than that of small pores. It provides a theoretical basis for the exploration and development of low-permeability sandstone reservoirs.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Borel Antony ◽  
Deltombe Raphaël ◽  
Moreau Philippe ◽  
Ingicco Thomas ◽  
Bigerelle Maxence ◽  
...  

AbstractDebates and doubt around the interpretation of use-wear on stone tools called for the development of quantitative analysis of surfaces to complement the qualitative description of traces. Recently, a growing number of studies showed that prehistoric activities can be discriminated thanks to quantitative characterization of stone tools surface alteration due to use. However, stone tool surfaces are microscopically very heterogeneous and the calculated parameters may highly vary depending on the areas selected for measurement. Indeed, it may be impacted by the effects from the raw material topography and not from the altered zones only, if non-altered part of the surface is included in the measurement. We propose here to discuss this issue and present a workflow involving the use of masks to separate worn and unworn parts of the surface. Our results show that this step of extraction, together with suitable filtering, could have a high impact on the optimization of the detection and thus characterization of use traces. This represents the basis for future automatic routines allowing the detection, extraction and characterization of wear on stone tools.


2021 ◽  
Vol 1 (2) ◽  
pp. 1-5
Author(s):  
Alfi Sophian ◽  
Andi Syukur

Analysis of the purity and concentration of isolated DNA in the manufacture of standard rat DNA was carried out to see whether the isolation carried out could produce good quality DNA. The purpose of this study is to provide information on the manufacture of raw DNA in species DNA testing where the raw material that has been purchased so far made from synthetic materials can be more economical if using DNA material derived from the meat raw material of the target species. The DNA extraction method used is the column spin method or column centrifuge using the Intron Patho Gene-Spin (Viral DNA/RNA) extraction kit. Analysis method of concentration and purity of isolated DNA was analyzed based on the average value of concentration and purity which was read using a nanophotometer. Based on the results of the research conducted, the results of the isolated DNA concentration values ​​were in the concentration range of 41,250 ng/ mL to 42,300 ng/mL, with the average concentration of isolated DNA was 41,777 ng/mL. For the value of the purity of the isolated DNA whose absorbance was read using a nanophotometer at a wavelength of A260/A280, the results were between 2,301 to 2,384 with the average value of purity being at 2,326. This study concludes that all the extracted samples that showed the results of the DNA analysis produced were included in the good DNA category.


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