Effect of Intramuscular Administration of Trypadim, Trypamidium and Novidium on Organ Damage and Packed Cell Volume of Wistar Rats Infected with Trypanosoma brucei brucei (Federe strain)

This study was aimed at determining the effect of leaves of Senna alata extract on biochemical indices of Wistar rats infected with Trypanosoma brucei brucei. Phytochemical screening revealed the absence of steroids in all extracts, absence of saponins in chloroform extracts and the presence of free anthraquinones only in chloroform extract. Post-infection treatment of animals stirred the emergence of parasitaemia by Day 3. Only animals receiving 200 mg/kg b.wt. of chloroform extract survived by day 16. A significant (P<0.05) decrease in ALT for groups receiving methanol (400 mg/kg b.wt.), chloroform and aqueous extracts and significant (P<0.05) increase in unconjugated bilirubin in the group receiving methanol extract (200 mg/kg b.wt.) compared to infected not treated rats. Significant (P<0.05) decrease in potassium concentration in groups receiving methanol and chloroform, and a significant (P<0.05) increase in sodium concentration in the group receiving 400 mg/kg b.wt. of aqueous extract compared to the infected not treated rats. These results thereby demonstrate the ameliorative potential of Senna alata leaves against T. brucei brucei.

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Flavonoid mixture ameliorates increase in erythrocyte osmotic fragility and malondialdehyde concentration induced by Trypanosoma brucei brucei-infection in Wistar rats

Research in Veterinary Science ◽

10.1016/j.rvsc.2013.10.005 ◽

2014 ◽

Vol 96 (1) ◽

pp. 139-142 ◽

Cited By ~ 10

Author(s):

Patricia I. Kobo ◽

Joseph O. Ayo ◽

Tagang Aluwong ◽

Abdulkadir U. Zezi ◽

Victor Maikai ◽

...

Keyword(s):

Trypanosoma Brucei ◽

Wistar Rats ◽

Osmotic Fragility ◽

Trypanosoma Brucei Brucei ◽

Erythrocyte Osmotic Fragility

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Haematological changes in Wistar rats experimentally infected with Trypanosoma congolense and Trypanosoma brucei brucei obtained from North-west Nigeria

Sokoto Journal of Veterinary Sciences ◽

10.4314/sokjvs.v19i2.1 ◽

2021 ◽

Vol 19 (2) ◽

pp. 73-80

Author(s):

A.O. Fajinmi ◽

O.O. Faleke ◽

A.A. Magaji ◽

U.M. Chafe ◽

M.A. Kassim ◽

...

Keyword(s):

Blood Cell ◽

Trypanosoma Brucei ◽

Wistar Rats ◽

Trypanosoma Congolense ◽

Control Group ◽

Blood Cell Count ◽

Trypanosoma Brucei Brucei ◽

Group A ◽

Haematological Changes ◽

Group D

This study determined haematological changes in Wistar rats experimentally infected with local strains of Trypanosoma congolense and Trypanosoma brucei brucei. Forty-five Wistar rats between 10 – 12 weeks old weighing between 210 – 240 g were used. The Wistar rats were randomly divided into four groups (A, B, C and D), with the infected groups (B, C and D) having 10 rats each, while the uninfected control group (A) had 15 rats. Group A rats were not infected and served as the control, group B were infected with Trypanosoma congolense, group C were infected with Trypanosoma brucei brucei and group D were co-infected with Trypanosoma congolense and Trypanosoma brucei brucei. Infection was achieved using 0.1mL of blood containing approximately 1 × 103 trypanosomes intraperitoneally into each Wistar rat in the infected groups. Clinical signs were observed. The changes in the blood cells were assayed in the groups post-infection. Duncan’s Least Square Deviation showed significantly (p<0.05) higher parasitaemia in infected groups. However, group D showed a higher significant (p<0.05) difference in parasitaemia when compared to groups B and C. The pattern of mean parasitaemia for the infected groups, revealed a positive correlation with days of post-infection (p<0.05) before the decline. The packed cell volume, total red blood cell count and haemoglobin concentration were significantly (p<0.05) lower in infected groups B, C and D. The total white blood cell count, platelet counts and differential leucocyte count were significantly (p<0.05) lower in infected groups when compared to the uninfected group. These findings suggest that co-infection with Trypanosoma congolense and Trypanosoma brucei brucei obtained from Wurno and Ngaski in Sokoto and Kebbi States respectively produced a more damaging effect on haematological parameters.

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The effect of tea on iron and aluminium metabolism in the rat

British Journal Of Nutrition ◽

10.1079/bjn19910066 ◽

1991 ◽

Vol 65 (1) ◽

pp. 61-68 ◽

Cited By ~ 37

Author(s):

Susan J. Fairweather-Tait ◽

Zoe Piper ◽

S. Jemil A. Fatemi ◽

Geoffrey R. Moore

Keyword(s):

Adverse Effect ◽

Cell Volume ◽

Packed Cell Volume ◽

Wistar Rats ◽

Fe Deficiency ◽

Tea Leaves ◽

Tea Infusion ◽

Synthetic Diet ◽

Male Wistar Rats ◽

Per Se

Weanling male Wistar rats were fed for 28 d on a semi-synthetic diet containing normal (38 μg/g) or low (9 μg/g) levels of iron. They were given water or tea infusion (20 g leaves/I water) to drink. Two further groups were given a normal- or low-Fe diet containing added tea leaves (20 g/kg diet). At the end of the study period, all rats given the low-Fe diet were severely anaemic, as assessed by haemoglobin, packed cell volume and liver Fe. Those given tea or the diet with added tea leaves showed a greater degree of Fe depletion. The blood and liver aluminium levels were not increased as a result of consuming tea or tea leaves, despite the higher Al intakes. Fe deficiencyper sehad no effect on Al absorption or retention from tea. It was concluded that the Al in tea was very poorly absorbed but that tea, either in the form of an infusion or as tea leaves, had an adverse effect on Fe status

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ANTI-ANAEMIC POTENTIAL OF METHANOLIC LEAF EXTRACT OF MUCUNA PRURIENS ON PHENYLHYDRAZINE (PHZ) INDUCED ANAEMIC ALBINO WISTAR RATS

FUDMA Journal of Sciences ◽

10.33003/fjs-2020-0403-399 ◽

2020 ◽

Vol 4 (3) ◽

pp. 370-374

Author(s):

Kasang Naman ◽

Habibat Oseni ◽

Emmanuel Enoh

Keyword(s):

Vitamin B12 ◽

Cell Volume ◽

Normal Control ◽

Normal Saline ◽

Packed Cell Volume ◽

Wistar Rats ◽

Leaf Extract ◽

Lethal Dose ◽

Mucuna Pruriens ◽

Control Group

The antianaemic potential of methanolic leaf extracts of Mucuna pruriens was investigated using phenylhydrazine (PHZ) induced anaemic albino Wistar rats. Fifteen rats used for the study were randomized into five experimental groups. To induced anaemia, the rats (except the normal control, Group E), received 60 mg/kg of the haemolytic agent Phenylhydrazine intraperitoneally (i.p) for two consecutive days. Anaemic Wistar rats in groups A and B received a daily oral dose of 500 and 250 mg/kg of the methanolic leaf extract of Mucuna pruriens. Nweze et al. (2016) had reported a median lethal dose greater than 5000 mg/kg for the methanol leaf extract of Mucuna pruriens. Groups C and D received Vitamin B12 (10 mg/kg) and normal saline (1 ml/kg), respectively. Normal control rats also received normal saline (1 ml/kg). Extract or normal saline was administered per os (p.o) while vitamin B12 was administered i.p. for a duration of 21 days. Packed cell volume (PCV) and haemoglobin concentration were determined weekly for three weeks. The result of the study indicated that both the methanolic leaf extract of Mucuna pruriens and Vitamin B12 significantly (p < 0.05) increased the packed cell volume and haemoglobin concentrations in treated rats compared to the negative control group of rats. This indicated that the methanolic leaf extract of Mucuna pruriens has anti-anaemic properties and could be utilized in the management of anaemia

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