scholarly journals ANTI-ANAEMIC POTENTIAL OF METHANOLIC LEAF EXTRACT OF MUCUNA PRURIENS ON PHENYLHYDRAZINE (PHZ) INDUCED ANAEMIC ALBINO WISTAR RATS

2020 ◽  
Vol 4 (3) ◽  
pp. 370-374
Author(s):  
Kasang Naman ◽  
Habibat Oseni ◽  
Emmanuel Enoh
Keyword(s):  
Vitamin B12 ◽  
Cell Volume ◽  
Normal Control ◽  
Normal Saline ◽  
Packed Cell Volume ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Lethal Dose ◽  
Mucuna Pruriens ◽  
Control Group

The antianaemic potential of methanolic leaf extracts of Mucuna pruriens was investigated using phenylhydrazine (PHZ) induced anaemic albino Wistar rats.  Fifteen rats used for the study were randomized into five experimental groups. To induced anaemia, the rats (except the normal control, Group E), received 60 mg/kg of the haemolytic agent Phenylhydrazine intraperitoneally (i.p) for two consecutive days. Anaemic Wistar rats in groups A and B received a daily oral dose of 500 and 250 mg/kg of the methanolic leaf extract of Mucuna pruriens. Nweze et al. (2016) had reported a median lethal dose greater than 5000 mg/kg for the methanol leaf extract of Mucuna pruriens. Groups C and D received Vitamin B12 (10 mg/kg) and normal saline (1 ml/kg), respectively. Normal control rats also received normal saline (1 ml/kg). Extract or normal saline was administered per os (p.o) while vitamin B12 was administered i.p. for a duration of 21 days. Packed cell volume (PCV) and haemoglobin concentration were determined weekly for three weeks. The result of the study indicated that both the methanolic leaf extract of Mucuna pruriens and Vitamin B12 significantly (p < 0.05) increased the packed cell volume and haemoglobin concentrations in treated rats compared to the negative control group of rats. This indicated that the methanolic leaf extract of Mucuna pruriens has anti-anaemic properties and could be utilized in the management of anaemia

scholarly journals Haemoglobin and Packed Cell Volume (PCV) of High-Fat Diet/Streptozotocine-Induced Diabetic Wistar Rats Treated with Ethanol Extract of a Herbal Mixture (Aju Mbaise)

2019 ◽  
pp. 1-6
Author(s):  
A. T. Nnadiukwu ◽  
C. C. Monago-Ighorodje ◽  
L. C. Chuku
Keyword(s):  
Cell Volume ◽  
Normal Control ◽  
Packed Cell Volume ◽  
High Fat Diet ◽  
Ethanol Extract ◽  
Diabetic Control ◽  
Control Group ◽  
High Fat ◽  
Herbal Mixture ◽  
Animal House

Aim: This study was carried out to evaluate the effect of ethanol extract of Aju Mbaise herbal mixture on some haematological indices of diabetic Wistar albino rats. Sample: Packed cell volume (PCV) and haemoglobin (Hb) concentration was estimated in diabetic rats treated with ethanol extract of Aju Mbaise herbal mixture. Study Design: In the course of the experiment, fifty-four (54) rats with initial weight range of 30 – 40 g were grouped into 6 of 9 rats per group. The first group served as the normal control (NC) while the remaining five groups were induced with diabetes type 2 using high-fat diet for 8 weeks and streptozotocin at 35 mg/kg body weight. Group II served as the diabetic control while the remaining groups (III, IV, V & VI) were treated with metformin and three different concentrations of the plant extract respectively. Place and Duration of Study: The study was carried out in the Animal house of the Department of Pharmacology, Faculty of Basic Clinical Sciences, University of Port Harcourt, between July 2018 and January 2019. Methodology: The haemoglobin and packed cell volume were estimated after 4th, 8th and 12th week of treatment using MINDRAY Auto-Haematology analyzer. Results: From the results obtained, it was observed that the diabetic control group has a PCV and haemoglobin concentration that is significantly (P<.05) lower when compared to that of the normal control group and the other treated groups. Conclusion: The study has shown that Aju Mbaise herbal mixture is a haematopoietic agent as it had the tendency to synthesize blood cells.

scholarly journals Effect of Ethanol Leaf Extract of Khat (Catha edulis forsk) on Prefrontal Cortex Oxidative Markers and Hematological Profile of Wistar Rats

2021 ◽  
pp. 149-162
Author(s):  
Solomon Adomi Mbina ◽  
Sanusi Bello Mada ◽  
Ejike Daniel Eze ◽  
Clement Olusoji Ajayi ◽  
Josiah Eseoghene Ifie ◽  
...  
Keyword(s):  
Prefrontal Cortex ◽  
Acute Toxicity ◽  
Study Design ◽  
Crude Extract ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Lethal Dose ◽  
Control Group ◽  
Catha Edulis ◽  
Oxidative Markers

Aim: This study evaluated the effect of ethanol leaf extract of Catha edulis on the prefrontal cortex oxidative markers and hematological profile of Wistar rats. Study Design: The study design was experimental. Place and Duration of Study: Department of Biochemistry, Kampala International University, Uganda and Faculty of Medicine, Department of Pharmacy, Mbarara University of Science and Technology, Mbarara, Uganda. Between January and May, 2021. Methodology: Fresh C. edulis leaves were collected after on-spot identification, and authenticated before being chopped into small pieces and pulverized mechanically. The powdered material was extracted through cold maceration using 70% ethanol for 72 h. The extract was concentrated in vacuo to dryness at 40oC using rotary evaporator. The lethal dose (LD50) of the crude extract was determined using acute toxicity test. The sub-acute toxicity of the crude extract was evaluated by orally administering daily doses of 250, 500, and 1000 mg/kg to the male Wistar rats for 28 days. The animals were monitored for mortality, behavioral and physiological signs of toxicity. Superoxide dismutase (SOD), Catalase (CAT) activities and Malondialdehyde (MDA) levels were estimated using standard methods. The blood samples were analyzed using the automated hematology analyzer: Mindray BC-6000. Shenzhen, China. Results: The median lethal dose (LD50) of the C. edulis leaf extract was greater than 5000mg/kg b w. The SOD and CAT activities of C. edulis treated groups compared to the control group were significantly (p < 0.05) reduced, the malondialdehyde (MDA) levels were increased in C. edulis treated groups compared to the control group. There was no significant change in the hematological parameters, however, there were slight variations in the WBC and RBC counts in some C. edulis treated groups. Conclusion: Long term chewing of C. edulis could result in oxidative stress. It is therefore important for chronic C. edulis leaf chewers to take caution.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals GAMBARAN HISTOLOGIK AORTA TIKUS WISTAR DENGAN DIET LEMAK BABI SETELAH PEMBERIAN EKSTRAK DAUN PEPAYA

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Friska W. F. Panjaitan ◽  
Marie M. Kaseke ◽  
George N. Tanudjaja
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Foam Cells ◽  
Group Iv ◽  
Control Group ◽  
Histological Features ◽  
Group I ◽  
Group Ii ◽  
Lard Diet ◽  
Control Group Group

Abstract: Papaya leaves contain several antioxidants (flavonoid, tanin, and vitamin C) which have antiatherogenic effects that may inhibit the progression of an atherosclerotic lesion. One of the risk factors causing atherosclerosis is the consumption of food containing highly saturated fatty acids e.g. lard. Low density lipoproteins (LDL) accumulate within the intima and then are oxidized (LDL-ox). This LDL-ox is ingested by macrophages, resulting in foam-cell formation (early lesion of atherosclerosis). This study aimed to find out the histological features of the aorta of wistar rats having lard diets without the addition of the papaya leaf extract; having lard diets along with the papaya leaf extract; and having lard diets followed by papaya leaf extract. This was an experimental study on 16 wistar rats divided into 4 groups: group I without treatment (negative control group), group II lard diet for 14 days (positive control group), group III lard diet with papaya leaf extract for 14 days (treatment group I), and group IV lard diet for 14 days, and then followed by papaya leaf extract for 14 days (treatment group II). It was found that the aorta of group I showed adipose cells in the intima and media layers; group II and III showed foam cells in both layers; and group IV showed foam cells in fewer numbers than group II. Conclusion: The aorta histological features of wistar rats given lard diets for 14 days, with or without papaya leaf extract, showed foam cells in the intima and media layers. Papaya leaf extraxt added to lard diets had no effect on decreasing foam cells (no protective effect), meanwhile papaya leaf extract following lard diets showed a reduction of foam cells (therapeutic effect). Keywords: papaya leaf, lard dietary, foam cells, wistar rat.   Abstrak: Daun pepaya mengandung antioksidan (flavonoid, vitamin C) yang berefek anti-aterogenik, sehingga diharapkan dapat menghambat perkembangan lesi aterosklerosis. Salah satu faktor risiko penyebab aterosklerosis yaitu makanan yang berkandungan tinggi asam lemak jenuh, antara lain lemak babi. Konsumsi lemak jenuh berlebihan dapat mengganggu fungsi sel endotel, sehingga lipoprotein berdensitas rendah (LDL) dapat masuk dan menjadi LDL teroksidasi (LDL-oks). Makrofag menangkap LDL-oks dan menjadi sel busa (lesi dini aterosklerosis). Penelitian ini bertujuan untuk mengetahui gambaran histologik aorta tikus wistar dengan diet lemak babi tanpa pemberian ekstrak daun pepaya, diet lemak babi bersamaan pemberian ekstrak daun pepaya, dan setelah diet lemak babi dilanjutkan pemberian ekstrak daun pepaya. Penelitian ini bersifat eksperimental. Subyek penelitian terdiri dari 16 ekor tikus wistar yang dibagi menjadi empat kelompok: kelompok I tanpa perlakuan (kelompok kontrol negatif); kelompok II dengan diet lemak babi selama 14 hari (kelompok kontrol positif); kelompok III dengan diet lemak babi serta pemberian ekstrak daun pepaya selama 14 hari (kelompok perlakuan I); dan kelompok IV dengan diet lemak babi selama 14 hari, dilanjutkan pemberian ekstrak daun pepaya selama 14 hari (kelompok perlakuan II). Hasil penelitian memperlihatkan gambaran histologi aorta kelompok I tampak perlemakan; pada kelompok II terdapat sel-sel busa; pada kelompok III masih terdapat sel-sel busa; dan pada kelompok IV terdapat sel-sel busa, namun dalam jumlah yang lebih sedikit dibandingkan pada gambaran kelompok II. Simpulan: Tikus wistar dengan diet lemak babi selama 14 hari, baik dengan maupun tanpa ekstrak daun pepaya, memperlihatkan gambaran histologik adanya sel-sel busa pada tunika intima dan tunika media aorta. Pemberian ekstrak daun pepaya bersamaan dengan diet lemak babi tidak berefek menurunkan jumlah sel busa (tidak ada efek protektif) sedangkan pemberian ekstrak daun pepaya setelah diet lemak babi berefek mengurangi jumlah sel-sel busa yang terbentuk (efek terapi). Kata kunci: daun pepaya, diet lemak babi, sel-sel busa, tikus wistar.

scholarly journals Treatment of Diabetic Condition in Streptozotocin Induced Diabetic Wister Rats Using Food Blends Such as Unripe Plantain, Soybean and Ginger

2019 ◽  
pp. 1-12
Author(s):  
I. Iwanegbe ◽  
M. Suleiman ◽  
A. Jimah
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Lipid Profile ◽  
Cell Volume ◽  
Glucose Level ◽  
Normal Control ◽  
Packed Cell Volume ◽  
Diabetic Rats ◽  
Diabetic Patients

Aims: To investigate the effect of food blends (plantain, soybean and ginger) on the blood glucose, lipid profile and haematological indices on streptozotocin induced diabetic rats. Methodology: A total of 35 rats of mean body weight 219.07 g separated into7 groups (5 per group) where induced by a single intraperitoneal (I.P) injection of streptozotocin (0.1 g dissolved in 5 ml of freshly prepared sodium citrate buffer 0.1 M, pH 4.5) at a dose of 40 mg/kg body weight after fasting for 12 hours and fed with flours/blends. The flours were produced from plant materials for different treatments/blends (blend A=100% unripe plantain, B=80% unripe plantain, 14% soybean, 6% ginger, C=70% unripe plantain, 26% soybean, 4% ginger, D= 60% unripe plantain, 38% soybean, 2% ginger, E= 50% unripe plantain, 50% soybean) and the phytochemicals and minerals content were determined. Blood glucose was determined at 5 days interval for 25 days. Diabetes was confirmed in rats with blood glucose concentrations >200 mg/dl. After 25 days rats were anaesthetized with chloroform vapour and blood samples collected by cardiac puncture for haematology and lipid profile determination. Results: The results showed that unripe plantain, soya beans and ginger in adequate proportion(C=70% unripe plantain, 26% soybean, 4% ginger or D= 60% unripe plantain, 38% soybean, 2% ginger) could help to reduce blood glucose, improve haematological parameters and lipid profile. Significant reduction was observed in the blood glucose level of rats fed blends C and D from 286 to 85 mg/dl and 307 to 90 mg/dl respectively at the end of experiment. These results also demonstrated that the inclusion of ginger at 6% causes rise in blood glucose level. Total cholesterol (TC) increased in all the blends. However, the lowest concentration of TC was observed in blends C and D. The highest packed cell volume (60%) and Haemoglobin (20 g/dl) level observed in rats fed blend C was significantly higher than the normal control fed conventional feeds. The increase in packed cell volume (PCV) (50%) and Hb (17 g/dl) in diabetic rats demonstrated that the formulated blend C was able to raise PCV and Hb above 50% and 17 g/dl (Normal control NC) respectively. Significant increase (P<0.05) in low density lipoprotein cholesterol (LDLc) was also observed in all the blends with blend C having the least (4.0 mg/dl) close to NC (2.0 mg/dl). Conclusion: From the results it is evident that blend C will manage and improve the health status of diabetic patients.

scholarly journals THE COMPARISON OF RAMANIA (Bouea macrophylla Griff) AND BINJAI (Mangifera caesia) LEAVES EXTRACT GEL EFFECT ON COLLAGEN DENSITY

2021 ◽  
Vol 6 (2) ◽  
pp. 159
Author(s):  
Nabilah Nabilah ◽  
Irham Taufiqurrahman ◽  
Nolista Indah Rasyid
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Total Sample ◽  
Control Group ◽  
Control Group Design ◽  
Gel Effect ◽  
Index Measurement ◽  
Male Wistar Rats ◽  
Collagen Density ◽  
Post Hoc

Background: The extract of ramania Bouea macrophylla Griff) and binjai (Mangifera caesia) leaf have flavonoid compounds that function as antioxidants to balance the amount of Reactive Oxygen Species (ROS) in tissues and optimize wound healing by helping synthesis of hydroxyproline which is used as a collagen synthesis material. Objective: To compare the effect of 15% concentration of ramania leaf extract gel, 15% concentration of binjai leaf extract gel on collagen density in back incision wounds of male Wistar rats on day 7 and day 14. Methods: This study used a true experimental design with a posttest-only with control group design. The study sample used male wistar rats that were healthy and active, aged 2-3 months with a body weight of 250-300 grams. The total sample was 18 rats divided into 6 groups. The 15% concentration of ramania and binjai leaves was given topically, then the rats were euthanized on the 7th and 14th day. Collagen index measurement was using hydroxyproline concentration. Results: Two-way Anova data analysis showed a significant value of 0.00 (p<0.05), which means that there was a difference in effect between the treatment gel and wound day. Bonferroni Post Hoc test showed a significant value in all treatment gel groups. Conclusion: There is a difference in the effect of 15% concentration of ramania leaf extract gel and 15% concentration of binjai extract gel on collagen density. Binjai leaf extract gel at 15% concentration is more effective for collagen density than Ramania leaf extract at 15% concentration and placebo. Keywords : Binjai Leaf Extract Gel, Collagen, Hydroxyproline, Ramania Leaf Extract Gel.

scholarly journals Hypoglycemic and Toxic Effect of Morus mesozygia Leaf Extract on the Liver and Kidneys of Alloxan-Induced Hyperglycemic Wistar Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Michael Tirwomwe ◽  
Isaac Echoru ◽  
Richard Maseruka ◽  
Kyobe Ronald Kimanje ◽  
Wilson Byarugaba
Keyword(s):  
Toxic Effect ◽  
Total Protein ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Statistical Significance ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Histological Damage ◽  
Alloxan Monohydrate ◽  
Antidiabetic Effects

Purpose. We investigated the hypoglycemic and toxic effect of Morus mesozygia leaf extract on the liver and kidneys of alloxan-induced hyperglycemic wistar rats. Method. Phytochemical analysis was done. Diabetes was induced by the use of alloxan monohydrate in six groups of rats, i.e., 200 mg/kg, 400 mg/kg, 800 mg/kg, glibenclamide, normal saline, and normal control group. Blood glucose was measured at the time of inoculation, then at 1, 2, 3, and 4 hours after. After 14 days, rats were killed under anesthesia; blood collected for measurement of total protein, albumin, TAGs, cholesterol, AST, ALT, urea, and creatinine; and whole tissue of liver and kidneys used for histological studies. Results. The extract possessed antidiabetic effects between 400 mg/kg and 800 mg/kg doses, which we attributed to the presence of flavonoids, tannins, terpenoids, and amino acids. There was a drop in total protein and albumin with no statistical significance (P≥0.05). The changes in levels of ALT, TAGs, cholesterol, AST, creatinine, and urea were not statistically different from the standard diabetic drug. The extract was protective against histological damage as there were no significant lesions suggestive of toxicities in the liver and kidneys at doses below 800 mg/kg. Conclusion. We established credible evidence that Morus mesozygia leaf extract has hypoglycemic effects between 400 mg/kg and 800 mg/kg and that it is safe on the liver and kidneys of wistar rats at doses less than 800 mg/kg.

scholarly journals Effect of ad libitum intake of an electrolyte repository in horses that underwent a polo game

2018 ◽  
Vol 39 (1) ◽  
pp. 187
Author(s):  
Lorena Chaves Monteiro ◽  
José Dantas Ribeiro Filho ◽  
Waleska De Melo Ferreira Dantas ◽  
Bruna Maria Ribeiro ◽  
Micheline Ozana da Silva ◽  
...  
Keyword(s):  
Cell Volume ◽  
Packed Cell Volume ◽  
Chloride Concentration ◽  
Control Group ◽  
Significance Level ◽  
Group Water ◽  
Calcium Magnesium ◽  
Ad Libitum ◽  
Ad Libitum Intake ◽  
And Control

The objective of this study was to assess the effects of an electrolyte and energy repository on the hematologic and biochemical variables of horses after a polo game and compare them to the effects of a commercial electrolyte repository and water. Twelve healthy horses, aged 5 to 10 years, mean body weight 356.25 ± 25.6 kg and in training for polo games, were randomly divided into three groups of four animals each and received one of three treatments. Animals participated in a 7-minute polo game. At the end of the match, they were taken to individual stalls where they received treatments for spontaneous hydration for 6 hours. Treatments were Hydroelectrolytic and Energy Repository (RHE) containing sodium, chloride, potassium, calcium, magnesium, dextrose, maltodextrin and, sucrose; and Commercial Paste (PCO) containing calcium, fructooligosaccharides, glycine, magnesium, potassium, sodium, and ad libitum water. The control group (Water) received water. Blood samples were collected as follows: just before the beginning of exercise (T0), immediately after exercise (T1), 3 hours after the end of exercise (T3), and 6 hours after the end of exercise (T6). The volume of voluntary intake was measured at T3 and T6. Packed cell volume and serum concentrations of sodium, potassium, chloride, calcium, magnesium, phosphorus, creatinine, total protein, plasma glucose, and lactate were measured. Data were submitted to descriptive statistics (mean ± standard deviation), Lilliefors and Cochran & Bartlett tests, analysis of variance (ANOVA), and Tukey or Duncan tests at a 5% significance level. The net volume ingested by the RHE group was higher than the PCO and control groups. In all groups, a reduction in chloride concentration and increases in packed cell volume, protein, creatinine, glucose, and lactate concentrations were observed in T1. These variables returned to the values found at T0 throughout the rehydration phase (T3 and T6). It is concluded that the ingestion of the hydroelectrolytic energy repository does not cause alteration in the biochemical profile of the animals.

scholarly journals Uji Efek Analgesik Ekstrak Daun Jarak Pagar (Jatropha Curcas L.) terhadap Tikus Wistar (Rattus Norvegicus)

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Oras B.M. Yensenem ◽  
Jane Wuisan ◽  
Henoch Awaloei
Keyword(s):  
Jatropha Curcas ◽  
Rattus Norvegicus ◽  
Analgesic Effect ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Negative Control ◽  
Control Group ◽  
Tropical Country ◽  
Mean Values ◽  
Jatropha Curcas L

Abstract: Indonesia is a tropical country with the second largest biodiversity in the world after Brazil. Jatropha curcas (Jatropha curcas L.) was believed as a pain reliever (analgesic). This study was aimed to prove the analgesic effect of Jatropha curcas leaf extract on Wistar rats (Rattus norvegicus). This was an experimental study using hot stimulus method in the form of temperature of 65°C. Subjects were 15 male Wistar rats divided into 5 groups. Group 1, the negative control group; group 2, the positive control group given paracetamol 40 mg/kgBW; and 3 treatment groups (groups 3, 4, and 5) given Jatropha curcas extract in several doses (300 mg/kgBW, 600 mg/kgBW, and 1200mg/kgBW). Observation of Wistar rat respons (licking and jumping) was performed for 1 minute at minutes -30, 60, 90, and 120 after treatment. The results showed that the mean values of response against heat decreased in accordance to time and reached the lowest values at minute 120, except group 5 (the extract dose of 1200mg/kg BW) which reached the lowest value at minute 90. Overall, the lowest mean value was found in group 4 (the extract dose of 600mg/kg BW). Conclusion: Jatropha curcas (Jatropha curcas L.) leaf extract had an analgesic effect on Wistar rats (Rattus norvegicus).Keywords: Jatropha curcas L., Rattus norvegicus, analgesic Abstrak: Indonesia merupakan negara beriklim tropis dengan keaneka ragaman hayati terbesar kedua di dunia setelah Brazil. Penelitian ini bertujuan untuk mengetahui efek analgesik pemberian ekstrak daun jarak pagar (Jatropha curcas L.) pada tikus Wistar (Rattus norvegicus). Jenis penelitian ini ialah eksperimental dengan mengguna-kan metode rangsang panas berupa suhu 65°C. Subjek penelitian mengunakan hewan uji berupa tikus Wistar (Rattus norvegicus) jantan sebanyak 15 ekor yang dibagi menjadi 5 kelompok, yaitu: Kelompok kontrol negatif; kelompok kontrol positif yang diberikan parasetamol 40 mg/kgBB; dan 3 kelompok perlakuan yang diberikan ekstrak jarak pagar (Jatropha curcas L.), dengan dosis 300 mg/kgBB, 600 mg/kgBB, dan 1200mg/kgBB. Pengamatan terhadap respon tikus Wistar berupa jilat dan lompat dilakukan selama 1 menit pada menit ke-30, 60, 90, dan 120 setelah pemberian ekstrak jarak pagar. Hasil penelitian mendapatkan bahwa terdapat penurunan nilai rerata respons terhadap panas yang sesuai dengan perlangsungan waktu kecuali pada kelompok 5 (dosis ekstrak daun jarak pagar 1200mg/kg BB) yang mencapai nilai terendah pada menit ke-90. Secara keseluruhan, nilai rerata respons yang terendah didapatkan pada kelompok 4 (dosis ekstrak daun jarak pagar 600mg/kg BB) pada menit ke-120. Simpulan: Ekstrak daun jarak pagar (Jatropha curcas L.) memiliki efek analgesik terhadap tikus Wistar (Rattus norvegicus)Kata kunci: Jatropha curcas L., Rattus norvegicus, analgesic

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