Determination of phytochemical components of Origanum majorana leaf extract by GC-MS spectroscopy and their effect on flax seedling growth

Background: Plant allelopathy refers to the release of chemicals from plants or microorganisms into the environment, may have direct or indirect, beneficial or harmful effects on other plants or microorganisms. When plants grow in an unfavorable environment,more allelochemicals will be secreted and the expression of allelopathic effects will increase, giving plants a certain competitive advantage. Hairy vetch is one of the most promising allelopathic crops and the aqueous extract of hairy vetch has an inhibitory effect on the root length and seedling height of grass crops. The current study aimed to study the allelopathic effect of hairy vetch on alfalfa, and exploring an ecological method to remove the root system of alfalfa.Methods: In this experiment, the allelopathic effects of the seeds, stems and leaves, roots extracts and root exudates (0, 3, 6, 9 and 12 mg·mL-1) on the seed germination and seedling growth of alfalfa were evaluated. And the main allelopathic substances from the stem and leaf extract were isolated and identified using high performance liquid chromatography- mass spectrometry (HPLC-MS).Result: The results showed that all of the extracts can inhibit alfalfa seed germination and seedling growth and stem and leaf extract had the strongest inhibitory effect, especially for inhibiting the root growth. A main allelochemical substance, o-coumaric acid, was screened out and the root length of alfalfa was completely inhibited at 1.6 mg·mL-1 of o-coumaric acid. The findings of these experiments show hairy vetch has strong allelopathic effect on alfalfa and o-coumaric acid is a chemical growth inhibitor.

Download Full-text

Spectroscopic Determination of Functionalized Active Principles from Coleus aromaticus Benth Leaf Extract Using Ionic Liquids

International Journal of Advances in Chemical Engineering and Biological Sciences ◽

10.15242/ijacebs.er01160016 ◽

2016 ◽

Vol 3 (1) ◽

Keyword(s):

Ionic Liquids ◽

Leaf Extract ◽

Spectroscopic Determination ◽

Coleus Aromaticus

Download Full-text

Origanum majorana Leaf Extract

10.32388/h5a1ow ◽

2020 ◽

Author(s):

Keyword(s):

Leaf Extract ◽

Origanum Majorana

Download Full-text

Comparative allelopathic potential of metabolites of two Alternanthera species against germination and seedling growth of rice

Planta Daninha ◽

10.1590/s0100-83582014000100001 ◽

2014 ◽

Vol 32 (1) ◽

pp. 1-10 ◽

Cited By ~ 4

Author(s):

A. Mehmood ◽

A. Tanveer ◽

M.A. Nadeem ◽

Z.A. Zahir

Keyword(s):

Seedling Growth ◽

Leaf Extract ◽

Alternanthera Philoxeroides ◽

Infested Soil ◽

Aqueous Extracts ◽

Seedling Vigor ◽

Allelopathic Potential ◽

Plant Parts ◽

Vigor Index ◽

Aqueous Leaf Extract

A laboratory study was conducted to investigate the allelopathic effect of aqueous extracts of plant parts of Alternanthera philoxeroides and A. sessilis and soil incorporated residues on germination and seedling growth of rice (Oryza sativa). Aqueous extracts prepared from different plant parts of Alternanthera species delayed rice germination. Alternanthera philoxeroides and A. sessilis inhibited rice germination by 9-100% and 4-49%, respectively. Germination of rice seeds was reduced with increasing concentration of aqueous leaf extracts of both weed species. Early seedling growth (root and shoot lengths) and seedling vigor index were significantly reduced by 5% aqueous leaf extract compared with distilled water treated control. Germination, root and shoot lengths, root and shoot dry weights and seedling vigor index of rice were drastically reduced by 3 and 4% in residue infested soil compared with residue free soil. The inhibitory effect of A. philoxeroides in terms of germination and seedling growth of rice was greater than that of A. sessilis. Five percent aqueous leaf extract and 4% residue infested soil of A. philoxeroides caused complete failure of rice seed germination. Alternanthera philoxeroides contained water soluble phenolics, namely 4 hydroxy-3-methoxy benzoic acid (16.19 mg L-1) and m-coumaric acid (1.48 mg L-1), whereas Alternanthera sessilis was rich in chlorogenic acid (17.85 mg L-1), gallic acid (11.03 mg L-1) and vanillic acid (9.88 mg L-1). The study indicates that the allelopathic potential of Alternanthera species may play an important role in enhancing the invasiveness of these species and may suppress rice plants in the vicinity.

Download Full-text

Microbiological and Antioxidant Activity of Phenolic Compounds in Olive Leaf Extract

Molecules ◽

10.3390/molecules25245946 ◽

2020 ◽

Vol 25 (24) ◽

pp. 5946

Author(s):

Dragana Borjan ◽

Maja Leitgeb ◽

Željko Knez ◽

Maša Knez Hrnčič

Keyword(s):

Phenolic Compounds ◽

Leaf Extract ◽

Biological Activities ◽

Olive Leaf ◽

Olive Leaves ◽

Olive Leaf Extract ◽

Antimicrobial Potential ◽

Isolation And Characterization ◽

Wide Range

According to many reports, phenolic compounds isolated from olive leaves have very good biological activities, especially antimicrobial. Presently, the resistance of microorganisms to antibiotics is greater than ever. Therefore, there are numerous recent papers about alternative solutions for inhibiting their influence on human health. Olive leaf is studied as an important source of antimicrobials with low cost and used in medicine. Numerous publications on involving green technologies for isolation of active compounds from olive leaves have appeared over the past few decades. The present review reports on current knowledge of the most isolated phenolic compounds from olive leaf extract as well as methods for their isolation and characterization. This paper uses recent research findings with a wide range of study models to describe the antimicrobial potential of phenolic compounds. It also describes the vast range of information about methods for determination of antimicrobial potential focusing on effects on different microbes. Additionally, it serves to highlight the role of olive leaf extract as an antioxidants and presents methods for determination of antioxidant potential. Furthermore, it provides an overview of presence of enzymes. The significance of olive leaves as industrial and agricultural waste is emphasized by means of explaining their availability, therapeutic and nutritional effects, and research conducted on this field.

Download Full-text

Allelopathic effects of Rhynchosia capitata on germination and seedling growth of mungbean

Planta Daninha ◽

10.1590/s0100-83582013000300002 ◽

2013 ◽

Vol 31 (3) ◽

pp. 501-509 ◽

Cited By ~ 5

Author(s):

H.H. Ali ◽

A. Tanveer ◽

M.A. Nadeem ◽

M.M. Javaid ◽

M.S. Kashif ◽

...

Keyword(s):

Phenolic Acids ◽

Seedling Growth ◽

Leaf Extract ◽

Water Soluble ◽

Total Phenolic ◽

Leaf Extracts ◽

Seedling Vigour ◽

Water Extracts ◽

Whole Plant ◽

Vigour Index

Experiments were conducted to evaluate the allelopathic influence of Rhynchosia capitata on germination and seedling growth of mungbean (Vigna radiate) along with identification of the phytotoxic substances responsible for this activity. Water extracts of root, shoot, leaf, fruit and whole plant were prepared by soaking them in water in a ratio of 1:20 (w/v) for 24 h. All the extracts affected germination and seedling growth of mungbean, but higher inhibition was seen with R. capitata leaf water extracts. A linear decrease in the germination characteristics of mungbean was observed with the decrease in the concentration of leaf extract from 5% to 1%. The soil-incorporated residues (1-4% w/w) of R. capitata stimulated the growth of root and hypocotyl at low concentrations, while it inhibited their growth at higher concentrations. Rhynchosia capitata soil-incorporated residues (4% w/w) significantly reduced the seedling vigour index of mungbean in addition to their significant effect on total germination. A significant amount of water-soluble phenolic acids were found in R. capitata plant extracts. The content of total phenolic acids was higher in the leaf extract compared to that of the stem, fruit or root extracts. Two phenolic acids including vanillic acid and 4‑(hydroxymethyl) benzoic acid were found in R. capitata leaf extracts.

Download Full-text