scholarly journals Identification of lymphatic pathway involved in the spreading of prostate cancer by fluorescence navigation approach with intraoperatively injected indocyanine green

2013 ◽  
Vol 5 (4) ◽  
pp. 254
Author(s):  
Shogo Inoue ◽  
Hiroaki Shiina ◽  
Naoko Arichi ◽  
Yozo Mitsui ◽  
Takeo Hiraoka ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Ex Vivo ◽  
Lymphatic Vessels ◽  
Lymphatic Channel ◽  
De Se ◽  
Internal Iliac ◽  
Green Solution ◽  
Vaisseaux Lymphatiques ◽  
Lymphatic Pathway

Objective: The objective of this study was to identify lymphaticvessels draining from the prostate by using a fluorescence navigation(FN) system.Methods: Fourteen subjects were candidates for radical retropubicprostatectomy (RRP) and pelvic lymph node dissection (PLND).After an indocyanine green solution was injected into the prostateduring RRP, lymphatic vessels draining from the prostate were analyzedusing a FN system. After PLND based on lymphatic mappingby the FN system (in vivo probing) was performed in the externaliliac, obturator and internal iliac regions; the fluorescence of theremoved lymph nodes (LNs) was analyzed on the bench (ex vivoprobing).Results: Under in vivo and ex vivo probing, the fluorescence intensityof internal iliac nodes was greater than that of external iliacor obturator nodes.Conclusion: The current study suggests that using a FN systemafter injecting indocyanine green is a safe and rational approachfor detecting the lymphatic channel draining from the prostate.The major lymphatic pathway involved in the spreading of prostatecancer appears to relate to internal iliac LNs, which wouldmean that the standard PLND covering external iliac and obturatorregions would not keep the cancer from spreading.Objectif : L’objectif de l’étude était de repérer les vaisseaux lymphatiquesquittant la prostate à l’aide d’un système d’imagerie parfluorescence (IF).Méthodologie : Quatorze sujets devaient subir une prostatectomieradicale rétropubienne (PRR) et une lymphadénectomie pelvienne.Après injection d’une solution de vert d’indocyanine dansla prostate pendant la PRR, les vaisseaux lymphatiques drainant laprostate ont été analysés par IF. Une lymphadénectomie pelviennefondée sur la cartographie lymphatique par IF (exploration in vivo)a ensuite été réalisée dans les régions de la fosse iliaque externe,de l’obturateur et de la fosse iliaque interne; la fluorescence desganglions lymphatiques retirés a été analysée sans délai (explorationex vivo).Résultats : Lors de l’exploration in vivo et ex vivo, l’intensité dela fluorescence des ganglions iliaques internes était plus forte quecelle des ganglions iliaques externes ou des ganglions obturateurs.Conclusion : Cette étude porte à croire que l’IF après injectionde vert d’indocyanine est une méthode sûre et rationnelle pourrepérer les vaisseaux lymphatiques drainant la prostate. La principalevoie lymphatique de propagation du cancer de la prostatesemble être reliée aux ganglions lymphatiques iliaques internes, cequi signifie que la lymphadénectomie pelvienne standard retirantles ganglions iliaques externes et obturateurs n’empêcherait pasle cancer de se propager.

scholarly journals Identification of lymphatic pathway involved in the spread of bladder cancer: Evidence obtained from fluorescence navigation with intraoperatively injected indocyanine green

2013 ◽  
Vol 7 (5-6) ◽  
pp. 322 ◽  
Author(s):  
Shogo Inoue ◽  
Hiroaki Shiina ◽  
Yozo Mitsui ◽  
Hiroaki Yasumoto ◽  
Akio Matsubara ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Radical Cystectomy ◽  
Fluorescence Intensity ◽  
Ex Vivo ◽  
Lymphatic Vessels ◽  
Rational Approach ◽  
Lymphatic Channel ◽  
Internal Iliac ◽  
Lymphatic Pathway

Introduction: We identify lymphatic vessels draining from the bladderby using fluorescence navigation (FN) system.Methods: In total, 12 candidates for radical cystectomy and pelviclymph node dissection (PLND) were included in this study. Afteran indocyanine green (ICG) solution was injected into the bladderduring radical cystectomy, lymphatic vessels draining from thebladder were analyzed using a FN system. PLND was based onthe lymphatic mapping created from the FN measurements (in vivoprobing) in the external iliac, obturator and internal iliac regions;after PLND, the fluorescence of the removed lymph nodes (LNs)was analyzed on the bench (ex vivo probing).Results: There were no patients with complications associated withthe intravesical ICG injection. A lymphatic pathway along inferiorvesical vessels to internal iliac LNs was clearly illustrated in 7 cases.Under in-vivo probing, the fluorescence intensity of internal iliacnodes was greater than that of external iliac or obturator nodes.Under ex-vivo probing, the fluorescence intensity of internal iliacand obturator nodes was greater than that of external iliac nodes.Conclusions: Using an FN system after injecting ICG during a radicalcystectomy operation is a safe and rational approach to detectingthe lymphatic channel draining from the bladder.

Abstract 27: ApoA-I Improves Lymphatic Function Through a Platelet-Dependent Mechanism in Atherosclerotic Mice

2017 ◽  
Vol 37 (suppl_1) ◽  
Author(s):  
Andreea Milasan ◽  
François Dallaire ◽  
Gabriel Jean ◽  
Jean-Claude Tardif ◽  
Yahye Merhi ◽  
...  
Keyword(s):  
Lymphatic Vessel ◽  
Ex Vivo ◽  
Lymphatic Vessels ◽  
Lymphatic Transport ◽  
Lv Function ◽  
Experimental Conditions ◽  
Lymphatic Function ◽  
Beneficial Effects ◽  
Dependent Mechanism

Rationale: Lymphatic vessels (LVs) are now recognized as prerequisite players in the modulation of cholesterol removal from the artery wall in experimental conditions of plaque regression, and a particular attention has been brought on the role of the collecting LVs in early atherosclerosis-related lymphatic dysfunction. Whereas recent findings revealed that apoA-I restores the neovascularization capacity of the lymphatic system during tumor necrosis factor-induced inflammation, the effect of apoA-I on collecting LV function during atherosclerosis has not been tested. Objective: In the present study, we address whether and how apoA-I can enhance collecting LV function in atherosclerosis-associated lymphatic dysfunction. Methods and Results: A 6-week systemic treatment with lipid-free apoA-I enhanced lymphatic transport and abrogated collecting lymphatic vessel permeability in atherosclerotic Ldlr –/– mice when compared to control. As injection of apoA-I has been shown to protect wild-type mice against flow restriction-induced thrombosis, and that platelets are identified as key elements in the maintenance of lymphatic vessel integrity via their interaction with lymphatic endothelial cells (LECs), we have tested whether the effects of apoA-I could be mediated through a platelet-dependent mechanism. Our in vivo results show that apoA-I kinetics in lymph reflected that of blood. Ex vivo experiments performed with washed platelets isolated from mouse blood reveal that apoA-I decreased thrombin-induced but not podoplanin-induced platelet aggregation. Whereas this result suggests that apoA-I limits platelet thrombotic potential in blood but not in lymph, we demonstrate that treatment of human LECs with apoA-I increases the adhesion of bridge-like platelets on human LECs. Conclusions: Our results suggest that apoA-I can mediate beneficial effects on lymphatic function by promoting platelet adhesion to the lymphatic endothelium and consequently restore collecting LV integrity. Altogether, we bring forward a new pleiotropic role for apoA-I in lymphatic function and unveil new potential therapeutic targets for the prevention and treatment of atherosclerosis.

Fluorescence-guided surgery using indocyanine green in dogs with superficial solid tumours

2020 ◽  
Vol 187 (7) ◽  
pp. 273-273
Author(s):  
Sophie Favril ◽  
Eline Abma ◽  
Emmelie Stock ◽  
Nausikaa Devriendt ◽  
Bart Van Goethem ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Near Infrared ◽  
Ex Vivo ◽  
False Positive Rate ◽  
Tumour Tissue ◽  
Surrounding Tissue ◽  
Tumour Resection ◽  
Nirf Imaging ◽  
Tumour Delineation

BackgroundNear-infrared fluorescence (NIRF) imaging is a relatively novel technique that can aid surgeons during intraoperative tumour identification.MethodsNine canine oncology patients (five mammary gland tumours, three mast cell tumours and one melanoma) received intravenous indocyanine green (ICG). After 24 hours, tumours were resected and fluorescence intensities of tumours and surroundings were evaluated. Additional wound bed tissue was resected if residual fluorescence was present after tumour resection. Ex vivo, fluorescence-guided dissection was performed to separate tumour from surrounding tissue.ResultsIntraoperative NIRF-guided tumour delineation was feasible in four out of nine dogs. Wound bed imaging after tumour removal identified nine additional fluorescent lesions, of which four contained tumour tissue. One of these four true positive in vivo lesions was missed by standard-of-care inspection. Ex vivo fluorescence-guided tumour dissection showed a sensitivity of 72 per cent and a specificity of 80 per cent in discriminating between tumour and surrounding tissue.ConclusionThe value of ICG for intraoperative tumour delineation seems more limited than originally thought. Although NIRF imaging using ICG did identify remaining tumour tissue in the wound bed, a high false positive rate was also observed.

scholarly journals Mesenteric lymph flow in adult and aged rats

2011 ◽  
Vol 301 (5) ◽  
pp. H1828-H1840 ◽  
Author(s):  
Tony J. Akl ◽  
Takashi Nagai ◽  
Gerard L. Coté ◽  
Anatoliy A. Gashev
Keyword(s):  
Shear Stress ◽  
Wall Shear Stress ◽  
Ex Vivo ◽  
Lymphatic Vessels ◽  
Lymph Flow ◽  
Contraction Frequency ◽  
Stress Load ◽  
Wall Shear ◽  
Contractile Characteristics

The objective of study was to evaluate the aging-associated changes, contractile characteristics of mesenteric lymphatic vessels (MLV), and lymph flow in vivo in male 9- and 24-mo-old Fischer-344 rats. Lymphatic diameter, contraction amplitude, contraction frequency, and fractional pump flow, lymph flow velocity, wall shear stress, and minute active wall shear stress load were determined in MLV in vivo before and after Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME) application at 100 μM. The active pumping of the aged rat MLV in vivo was found to be severely depleted, predominantly through the aging-associated decrease in lymphatic contractile frequency. Such changes correlate with enlargement of aged MLV, which experienced much lower minute active shear stress load than adult vessels. At the same time, pumping in aged MLV in vivo may be rapidly increased back to levels of adult vessels predominantly through the increase in contraction frequency induced by nitric oxide (NO) elimination. Findings support the idea that in aged tissues surrounding the aged MLV, the additional source of some yet unlinked lymphatic contraction-stimulatory metabolites is counterbalanced or blocked by NO release. The comparative analysis of the control data obtained from experiments with both adult and aged MLV in vivo and from isolated vessel-based studies clearly demonstrated that ex vivo isolated lymphatic vessels exhibit identical contractile characteristics to lymphatic vessels in vivo.

scholarly journals Ex vivo Demonstration of Functional Deficiencies in Popliteal Lymphatic Vessels from TNF-Tg Mice with Inflammatory Arthritis

2020 ◽  
Author(s):  
Joshua P. Scallan ◽  
Echoe M. Bouta ◽  
Homaira Rahimi ◽  
H. Mark Kenney ◽  
Christopher T. Ritchlin ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Repeated Measures ◽  
Inflammatory Arthritis ◽  
Ex Vivo ◽  
Lymphatic Vessels ◽  
Chronic Inflammatory Arthritis ◽  
Wide Range ◽  
Nos Inhibitors ◽  
Draining Lymph Nodes

AbstractBackgroundRheumatoid arthritis (RA) is a progressive immune-mediated inflammatory disease characterized by intermittent episodes of pain and inflammation in affected joints, or flares. Recent studies demonstrated lymphangiogenesis and expansion of draining lymph nodes during chronic inflammatory arthritis, and lymphatic dysfunction associated with collapse of draining lymph nodes in RA patients and TNF-transgenic (TNF-Tg) mice experiencing arthritic flare. As the intrinsic differences between lymphatic vessels afferent to healthy, expanding, and collapsed draining lymph nodes are unknown, we characterized the ex vivo behavior of popliteal lymphatic vessels (PLVs) from WT and TNF-Tg mice. We also interrogated the mechanisms of lymphatic dysfunction through inhibition of nitric oxide synthase (NOS).MethodsPopliteal lymph nodes (PLNs) in TNF-Tg mice were phenotyped as Expanding or Collapsed by in vivo ultrasound and age-matched to WT littermate controls. The PLVs were harvested and cannulated for ex vivo functional analysis over a relatively wide range of hydrostatic pressures (0.5 to 10 cmH2O) to quantify the end diastolic diameter (EDD), tone, amplitude (AMP), ejection fraction (EF), contraction frequency (FREQ) and fractional pump flow (FPF) with or without NOS inhibitors Data was analyzed using repeated measures two-way ANOVA with Bonferroni’s post hoc test.ResultsReal time videos of the cannulated PLVs demonstrated the predicted phenotypes of robust versus weak contractions of the WT versus TNF-Tg PLV, respectively. Quantitative analyses confirmed that TNF-Tg PLVs had significantly decreased AMP, EF and FPF versus WT (p<0.05). EF and FPF were recovered by NOS inhibition, while the reduction in AMP was NOS independent. No differences in EDD, tone, or FREQ were observed between WT and TNF-Tg PLVs, nor between Expanding versus Collapsed PLVs.ConclusionThese findings support the concept that chronic inflammatory arthritis leads to NOS dependent and independent draining lymphatic vessel dysfunction that exacerbates disease, and may trigger arthritic flare due to decreased egress of inflammatory cells and soluble factors from affected joints.

scholarly journals Optimizing Indocyanine Green Fluorescence Angiography in Reconstructive Flap Surgery: A Systematic Review and Ex Vivo Experiments

2019 ◽  
Vol 27 (1) ◽  
pp. 103-119 ◽  
Author(s):  
Tim Pruimboom ◽  
Sander M. J. van Kuijk ◽  
Shan S. Qiu ◽  
Jacqueline van den Bos ◽  
Fokko P. Wieringa ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Fluorescence Intensity ◽  
Ex Vivo ◽  
Tissue Perfusion ◽  
Fluorescence Angiography ◽  
Fixed Dose ◽  
Flap Surgery ◽  
Comprehensive Overview ◽  
Indocyanine Green Fluorescence

Background. Indocyanine green angiography (ICGA) offers the potential to provide objective data for evaluating tissue perfusion of flaps and reduce the incidence of postoperative necrosis. Consensus on ICGA protocols and information on factors that have an influence on fluorescence intensity is lacking. The aim of this article is to provide a comprehensive insight of in vivo and ex vivo evaluation of factors influencing the fluorescence intensity when using ICGA during reconstructive flap surgery. Methods. A systematic literature search was conducted to provide a comprehensive overview of currently used ICGA protocols in reconstructive flap surgery. Additionally, ex vivo experiments were performed to further investigate the practical influence of potentially relevant factors. Results. Factors that are considered important in ICGA protocols, as well as factors that might influence fluorescence intensity are scarcely reported. The ex vivo experiments demonstrated that fluorescence intensity was significantly related to dose, working distance, angle, penetration depth, and ambient light. Conclusions. This study identified factors that significantly influence the fluorescence intensity of ICGA. Applying a weight-adjusted ICG dose seems preferable over a fixed dose, recommended working distances are advocated, and the imaging head during ICGA should be positioned in an angle of 60° to 90° without significantly influencing the fluorescence intensity. All of these factors should be considered and reported when using ICGA for tissue perfusion assessment during reconstructive flap surgery.

Application of Albumin Protein and Indocyanine Green Chromophore for Tissue Soldering by Using an IR Diode Laser: Ex Vivo and In Vivo Studies

2010 ◽  
Vol 28 (6) ◽  
pp. 723-733 ◽  
Author(s):  
M.E. Khosroshahi ◽  
M.S. Nourbakhsh ◽  
S. Saremi ◽  
A. Hooshyar ◽  
Sh. Rabbani ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Diode Laser ◽  
Ex Vivo ◽  
In Vivo Studies

scholarly journals A novel indocyanine green-based fluorescent marker for guiding surgical tumor resection

2021 ◽  
pp. 2150013
Author(s):  
Jiawei Ge ◽  
Justin D. Opfermann ◽  
Hamed Saeidi ◽  
Katherine A. Huenerberg ◽  
Christopher D. Badger ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Feasibility Study ◽  
Near Infrared ◽  
Ex Vivo ◽  
Tumor Resection ◽  
Fluorescent Marker ◽  
India Ink ◽  
Long Term Treatment ◽  
Mouse Study

Surgical tumor resection is a common approach to cancer treatment. India Ink tattoos are widely used to aid tumor resection by localizing and mapping the tumor edge at the surface. However, India Ink tattoos are easily obscured during electrosurgical resection, and fade in intensity over time. In this work, a novel near-infrared (NIR) fluorescent marker is introduced as an alternative. The NIR marker was made by mixing indocyanine green (ICG), biocompatible cyanoacrylate, and acetone. The marking strategy was evaluated in a chronic ex vivo feasibility study using porcine tissues, followed by a chronic in vivo mouse study while compared with India Ink. In both studies, signal-to-noise (SNR) ratios and dimensions of the NIR markers and/or India Ink over the study period were calculated and reported. Electrocautery was performed on the last day of the mouse study after mice were euthanized, and SNR ratios and dimensions were quantified and compared. Biopsy was performed at all injection sites and slides were examined by a pathologist. The proposed NIR marker achieved (i) consistent visibility in the 26-day feasibility study and (ii) improved durability, visibility, and biocompatibility when compared to traditional India Ink over the six-week period in an in vivo mouse model. These effects persist after electrocautery whereas the India Ink markers were obscured. The use of a NIR fluorescent presurgical marking strategy has the potential for intraoperative tracking during long-term treatment protocols.

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