Bioengineered Silver Nanoparticle from Spirulina platensis in Attenuating Biofilm Mediated Virulence in Vibrio parahemolyticus: An in vitro and in vivo Approach

The initial interaction and adherence of Vibrio parahemolyticus to epithelial tissue culture cells, human buccal epithelial cells, and the ileal mucosa of mice were studied. Using scanning electron microscopy, adherent bacteria were observed only on degenerating human embryonic intestinal, HeLa, and buccal cells; healthy normal cells were devoid of bacteria. Sheared V. parahaemolyticus, i.e., lacking flagella, did not adhere to either normal or degenerating tissue cells. Neither ultraviolet-inactivated organisms nor cell-free culture supernate affected the epithelial cells. Similar findings were observed on the mucosa of the ileum in mice inoculated with V. parahaemolyticus. It appears that V. parahaemolyticus possesses a cytotoxic factor which alters epithelial cells. This factor appears to be closely associated with viable organisms and may be a functional element in the adherence process of flagellated V. parahaemolyticus to mammalian epithelial cells.

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Formulation of Chitosan Stabilized Silver Nanoparticle-Containing Wound Healing Film: In Vitro and In Vivo Characterization

Journal of Pharmaceutical Sciences ◽

10.1016/j.xphs.2020.03.028 ◽

2020 ◽

Vol 109 (7) ◽

pp. 2196-2205 ◽

Cited By ~ 3

Author(s):

Chintan Pansara ◽

Renuka Mishra ◽

Tejal Mehta ◽

Ankit Parikh ◽

Sanjay Garg

Keyword(s):

Wound Healing ◽

Silver Nanoparticle ◽

In Vivo Characterization

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Perendaman Ekstrak Spirulina plantesis Terhadap Ig-M, Jaringan Limpa dan Diferensial Leukosit Ikan Mas Setelah Diinfeksi Aeromonas hydrophila (Deeping Of Extract Spirulina plantesis To Ig-M, Spleen Tissue And Diferential Leucocyte Of Carp After Infected By Aeromonas hydrophila)

Jurnal Biosains Pascasarjana ◽

10.20473/jbp.v18i3.2016.218-229 ◽

2016 ◽

Vol 18 (3) ◽

pp. 218

Author(s):

Widya Pratiwi

Keyword(s):

Aeromonas Hydrophila ◽

Spirulina Platensis ◽

Spleen Tissue

Abstrak Penyakit yang sering menyerang ikan mas yaitu bakteri Aeromonas hydrophila, bakteri ini penyebab penyakit bercak merah atau Haemerrhagic Septicaemia pada ikan air tawar. Salah satu bahan alami yang dapat digunakan untuk pengendalian sistem tubuh terhadap penyakit yaitu Spirulina platensis. Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak etanol Spirulina platensis terhadap respon Ig-M, diferential leukosit, histopatologi limpa yang diinfeksi bakteri Aeromonas hydrophila. Penelitian ini dilakukan dalam dua tahap uji, yaitu uji in vitro dan uji in vivo. Pada uji in vivo metode yang digunakan adalah eksperiment laboratoris dengan menggunakan 5 perlakuan yaitu kontrol positif (ikan diinfeksi A. hydrophila), kontrol negatif (ikan diberi Phospate Buffer Saline ), perendaman ekstrak dosis 50 ppm, dosis 100 ppm, dosis 200 ppm. Ikan mas diinfeksi terlebih dahulu dengan cara menyuntikkan 0,1 ml suspense A. hydrophila sebanyak 106 cfu/ml secara intramuskular. Setelah menunjukkan gejala klinis selama 2 hari, ikan direndam dengan ekstrak etanol S. platensis selama 30 menit. Perendaman dilakukan dua kali, pada hari ke 2 dan ke 5. Hasil penelitian menunjukkan bahwa ekstrak S. platensis memberikan pengaruh yang berbeda nyata (p<0.05) terhadap kondisi jaringan limpa, diferensial leukosit dan konsentrasi Ig-M. Ekstrak S. platensis dapat meningkatkan sistem kekebalan tubuh denga adanya peningkatan konsentrasi Ig-M dibentuk oleh proliferasi limfosit sel B. Dosis perendaman yang sudah dapat meningkatkan sistem kekebaln tubuh adalah 100 ppm dengan tingkat kelangsungan hidup tertinggi 88,8%.Kata kunci : S. platensis, A. hydrophila, histopatologi, Ig-M, diferensial leukosit

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In Situ Silver-based Electrochemical Bioreactor In Vivo

10.21203/rs.3.rs-103588/v1 ◽

2020 ◽

Author(s):

Yong Huang ◽

Liping Zhong ◽

Zhiming Deng ◽

Pan Wu ◽

Jian He ◽

...

Keyword(s):

Silver Nanoparticles ◽

Graphene Oxide ◽

Tumor Cells ◽

Silver Nanoparticle ◽

A549 Cells ◽

Induced Apoptosis ◽

Induced Changes

Abstract In this study we show for the first time that a reduced graphene oxide (rGO) carrier has a 15-fold higher catalysis rate than graphene oxide (GO) in Ag+ reduction. Based on this, we constructed a tumor microenvironment-enabled in situ silver-based electrochemical oncolytic bioreactor (SEOB) which unlocked an Ag+ prodrug to generate silver nanoparticles and inhibited the growth of various tumors. In this bioreactor system, intratumoral H2O2 acted as the reductant and the rGO carrier acted as the catalyst. Chelation of aptamers to this prodrug increased the production of silver nanoparticles by tumor cells, especially in the presence of Vitamin C, which broke down in tumor cells to supply massive amounts of H2O2. Consequently, highly efficient silver nanoparticle-induced apoptosis was observed in HepG2 and A549 cells in vitro and in HepG2- and A549-derived tumors in vivo. The apoptosis was associated with ROS-induced changes in mitochondrial membrane potential and DNA damage. The specific aptamer targeting and intratumoral silver nanoparticle production guaranteed excellent biosafety, with no damage to normal cells, because the Ag+ prodrug was specifically unlocked in tumors. More significantly, there was no evident tissue damage in monkeys, which greatly increases the clinical translation potential of the SEOB system.

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