scholarly journals Perendaman Ekstrak Spirulina plantesis Terhadap Ig-M, Jaringan Limpa dan Diferensial Leukosit Ikan Mas Setelah Diinfeksi Aeromonas hydrophila (Deeping Of Extract Spirulina plantesis To Ig-M, Spleen Tissue And Diferential Leucocyte Of Carp After Infected By Aeromonas hydrophila)

2016 ◽  
Vol 18 (3) ◽  
pp. 218
Author(s):  
Widya Pratiwi
Keyword(s):  
Aeromonas Hydrophila ◽  
Spirulina Platensis ◽  
Spleen Tissue

Abstrak            Penyakit yang sering menyerang ikan mas yaitu bakteri Aeromonas hydrophila, bakteri ini penyebab penyakit bercak merah atau Haemerrhagic Septicaemia pada ikan air tawar. Salah satu bahan alami yang dapat digunakan untuk pengendalian sistem tubuh terhadap penyakit yaitu Spirulina platensis.  Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak etanol Spirulina platensis terhadap respon Ig-M, diferential leukosit, histopatologi limpa yang diinfeksi bakteri Aeromonas hydrophila. Penelitian ini dilakukan dalam dua tahap uji, yaitu uji in vitro dan uji in vivo. Pada uji in vivo metode yang digunakan adalah eksperiment laboratoris dengan menggunakan 5 perlakuan yaitu kontrol positif (ikan diinfeksi A. hydrophila), kontrol negatif (ikan diberi Phospate Buffer Saline ), perendaman ekstrak dosis 50 ppm, dosis 100 ppm, dosis 200 ppm. Ikan mas diinfeksi terlebih dahulu dengan cara menyuntikkan 0,1 ml suspense A. hydrophila sebanyak 106 cfu/ml secara intramuskular. Setelah menunjukkan gejala klinis selama 2 hari, ikan direndam dengan ekstrak etanol S. platensis selama 30 menit. Perendaman dilakukan dua kali, pada hari ke 2 dan ke 5. Hasil penelitian menunjukkan bahwa ekstrak S. platensis memberikan pengaruh yang berbeda nyata (p<0.05) terhadap kondisi jaringan limpa, diferensial leukosit dan konsentrasi Ig-M. Ekstrak S. platensis dapat meningkatkan sistem kekebalan tubuh denga adanya peningkatan konsentrasi Ig-M dibentuk oleh proliferasi limfosit sel B. Dosis perendaman yang sudah dapat meningkatkan sistem kekebaln tubuh adalah 100 ppm dengan tingkat kelangsungan hidup tertinggi 88,8%.Kata kunci : S. platensis, A. hydrophila, histopatologi, Ig-M, diferensial leukosit

scholarly journals Attenuation of Aeromonas hydrophila Infection in Carassius auratus by YtnP, a N-acyl Homoserine Lactonase from Bacillus licheniformis T-1

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 631
Author(s):  
Mengfan Peng ◽  
Wentao Tong ◽  
Zhen Zhao ◽  
Ling Xiao ◽  
Zhaoyue Wang ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Bacillus Licheniformis ◽  
Aeromonas Hydrophila ◽  
Biofilm Formation ◽  
Carassius Auratus ◽  
Quorum Quenching ◽  
Inhibition Rate ◽  
Sequence Identity

In this experiment, the quorum quenching gene ytnP of Bacillus licheniformis T-1 was cloned and expressed, and the effect against infection of Aeromonas hydrophila ATCC 7966 was evaluated in vitro and vivo. The BLAST results revealed a 99% sequence identity between the ytnP gene of T-1 and its homolog in B.subtilis sub sp. BSP1, and the dendroGram showed that the similarity in the YtnP protein in T-1 was 100% in comparison with B.subtilis 3610, which was categorized as the Aidc cluster of the MBL family. The AHL lactonase activity of the purified YtnP was detected as 1.097 ± 0.7 U/mL with C6-HSL as the substrate. Otherwise, purified YtnP protein could significantly inhibit the biofilm formation of A.hydrophila ATCC 7966 with an inhibition rate of 68%. The MIC of thiamphenicol and doxycycline hydrochloride against A. hydrophila reduced from 4 μg/mL and 0.5 μg/mL to 1 μg/mL and 0.125 μg/mL, respectively, in the presence of YtnP. In addition, YtnP significantly inhibited the expression of five virulence factors hem, ahyB, ast, ep, aerA of A. hydrophila ATCC 7966 as well (p < 0.05). The results of inhibition on virulence showed a time-dependence tendency, while the strongest anti-virulence effects were within 4–24 h. In vivo, when the YtnP protein was co-injected intraperitoneally with A. hydrophila ATCC 7966, it attenuated the pathogenicity of A. hydrophila and the accumulated mortality was 27 ± 4.14% at 96 h, which was significantly lower than the average mortality of 78 ± 2.57% of the Carassius auratus injected with 108 CFU/mL of A. hydrophila ATCC 7966 only (p < 0.001). In conclusion, the AHL lactonase in B. licheniformis T-1 was proven to be YtnP protein and could be developed into an agent against infection of A. hydrophila in aquaculture.

Sulfated galactan from Halymenia dilatata enhance the antioxidant properties and prevents Aeromonas hydrophila infection in tilapia fish: In vitro and in vivo study

2020 ◽  
Vol 158 ◽  
pp. 569-579
Author(s):  
Manoharan Vinosha ◽  
Subramanian Palanisamy ◽  
Ravichandran Anjali ◽  
Changsheng Li ◽  
Khamphone Yelithao ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Antioxidant Properties ◽  
In Vivo Study ◽  
Sulfated Galactan

In vitro and in vivo efficacy of rosmarinic acid on quorum sensing mediated biofilm formation and virulence factor production in Aeromonas hydrophila

Biofouling ◽  
2016 ◽  
Vol 32 (10) ◽  
pp. 1171-1183 ◽  
Author(s):  
Kannan Rama Devi ◽  
Ramanathan Srinivasan ◽  
Arunachalam Kannappan ◽  
Sivasubramanian Santhakumari ◽  
Murugan Bhuvaneswari ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Virulence Factor ◽  
Aeromonas Hydrophila ◽  
Rosmarinic Acid ◽  
Biofilm Formation ◽  
In Vivo Efficacy ◽  
Factor Production ◽  
Virulence Factor Production

scholarly journals The use of Cattapa Leaves Terminalia cattapa as Preventive and Curative Methods in Patin Catfish Pangasionodon hypophthalmus Infected With Aeromonas hydrophila

2008 ◽  
Vol 7 (1) ◽  
pp. 79 ◽  
Author(s):  
D. Wahjuningrum ◽  
N. Ashry ◽  
Sri Nuryati
Keyword(s):  
Freshwater Fish ◽  
Clinical Sign ◽  
Aeromonas Hydrophila ◽  
Susceptibility Test ◽  
Blood Picture ◽  
Antibacterial Test ◽  
Test Fish

<p>Motile Aeromonad Septicaemia (MAS) caused by <em>Aeromonas hydrophila</em> induced serious epidemics of ulcerative disease in freshwater fish including patin catfish <em>Pangasionodon hypophthalmus</em>. <em>In vitro</em> study for antibacterial test of cattapa leaves <em>Terminalia cattapa</em> (TC) were done previous to the <em>in vivo</em> test. The <em>in vitro</em> susceptibility test was performed at the dosages of 30, 60 and 90 g/l TC. At the <em>in vivo</em> test, fish were injected intramusculary with TC at the dosages of 60 g/l for the prevention and 120 g/l for curative efficacy. Results from blood picture, clinical sign and mortaliyt showed that TC were better and more effective as preventive than curative for MAS in patin catfish.</p> <p>Keywords :  <em>Terminalia cattapa</em>, <em>Aeromonas hydrophila</em>, patin catfish</p> <p> </p> <p>ABSTRAK</p> <p>Penyakit MAS (<em>Motile Aeromonad Septicaemia</em>) yang disebabkan oleh <em>Aeromonas hydrophila</em> sering menyebabkan wabah penyakit tukak pada ikan-ikan air tawar termasuk pada ikan patin <em>Pangasionodon hypophthalmus</em>. Uji antibakteri secara <em>in vitro</em> dari daun ketapang <em>Terminalia cattapa</em> (TC) dilakukan sebelum uji <em>in vivo</em>. Pada uji <em>in vitro</em> dilakukan pengujian aktivitas antibakteri TC terhadap <em>A. Hydrophila</em> pada dosis TC 30, 60 dan 90 g/l. Pada uji <em>in vivo</em>, ikan diinfeksi secara intramuskular dengan TC, untuk pencegahan dengan dosis 60 g/l TC dan pengobatan pada dosis 120 g/l TC. Hasil yang diperoleh dari gambaran darah, gejala klinis dan kematian ikan patin menunjukkan bahwa TC lebih baik dan sangat efektif untuk pencegahan daripada pengobatan untuk penyakit MAS pada ikan patin.</p> Kata kunci: <em>Terminalia cattapa</em>, <em>Aeromonas hydrophila</em>, ikan patin

scholarly journals Screening of Probiotic Bacteria from Intestine and Culture Environment of Hoeven’s slender carp Leptobarbus hoeveni Blkr to Control Pathogenic Bacteria

2010 ◽  
Vol 9 (2) ◽  
pp. 127
Author(s):  
. Sunarto ◽  
. Sukenda ◽  
. Widanarni
Keyword(s):  
Aeromonas Hydrophila ◽  
Pathogenic Bacteria ◽  
Probiotic Bacteria ◽  
Streptococcus Iniae ◽  
Mycobacterium Fortuitum ◽  
Fish Pathogen ◽  
Culture Environment ◽  
Control Disease

The ability of probiotic bacteria to control disease infection has been used in aquaculture. This experiment was conducted to isolate and characterize probiotic bacteria; the competition test its ability probiotic bacteria against pathogenic bacteria; and to improve survival rate of Leptobarbus hoeveni. The bacteria were isolated from Leptobarbus hoeveni and its culture environment, and then tested to know its ability to inhibit bacterial fish pathogen in-vitro. Furthermore, the selected probiotic bacteria were tested in vivo to evaluate their ability to inhibit pathogen of Leptobarbus hoeveni.  The result showed that probiotic bacteria inhibit the growth of Streptococcus iniae, Flexibacter columnaris, Mycobacterium fortuitum and Aeromonas hydrophila in vitro.  Isolate DD3 was the best of candidate probiotic because of the ability to inhibit pathogen, especially A. hydrophila, the most virulent bacteria in Leptobarbus hoeveni.<br /><br />Key Words  : probiotic bacteria, Leptobarbus hoeveni, pathogenic bacteria<br /><br />Abstrak<br /><br />Kemampuan bakteri probiotik untuk mengendalikan penyakit infeksi telah digunakan dalam akuakultur. Tujuan penelitian ini adalah mengisolasi dan mengkarakterisasi bakteri probiotik, menguji kemampuan bakteri probiotik terhadap bakteri patogen, sehingga dapat meningkatkan tingkat kelangsungan hidup ikan jelawat. Bakteri diisolasi dari usus ikan jelawat dan lingkungan budaya, kemudian diuji kemampuannya menghambat bakteri patogen secara in-vitro. Selanjutnya bakteri probiotik yang dipilih diuji secara in vivo untuk mengevaluasi kemampuannya dalam menghambat patogen di dalam tubuh ikan jelawat. Dari hasil penelitian diperoleh bakteri probiotik yang diisolasi dari usus dan lingkungan budaya ikan jelawat menunjukkan penghambatan pertumbuhan terhadap Streptococcus iniae, Flexibacter columnaris, Mycobacterium fortuitum dan Aeromonas hydrophila secara in vitro. Isolat DD3 merupakan kandidat probiotik terbaik, karena mempunyai kemampuan untuk menghambat bakteri patogen,  khususnya bakteri  A. hydrophila adalah bakteri yang paling viluren bagi ikan jelawat.<br />    <br />Kata Kunci:   bakteri probiotik, ikan jalawat dan baktri patogen<br />

scholarly journals Óleo essencial da Canela (Cinamaldeído) e suas aplicações biológicas.

2018 ◽  
Vol 9 (2) ◽  
pp. 192
Author(s):  
Cristiane Santos Silva e Silva Figueiredo ◽  
Patrícia Viera de Oliveira ◽  
Warlison Felipe De Silva Saminez ◽  
Roseana Muniz Diniz ◽  
João Francisco Silva Rodrigues ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Aeromonas Hydrophila ◽  
Clostridium Botulinum ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Escherichia Coli O157 ◽  
Electronic Library ◽  
De Se ◽  
Serovar Typhimurium

Introdução: Cinamaldeído (CND) é o principal componente ativo do óleo essencial da canela (Cinnamomum sp) e tem sido amplamente utilizado em atividades biológicas e farmacológicas, tendo sido relatado atividade antimicrobiana, antioxidante, antidiabética, dentre outras. Objetivo: Devido os diversos relatos das propriedades farmacológicas do composto, esse foi escolhido para revisão de literatura. A seleção da bibliografia foi obtida a partir de bases de dados (google scholar, NCBI - National Center for Biotechnology Information, PubMed e Scielo - Scientific Electronic Library Online). Discussão: A composição química do CND tem compostos terpenóides que têm poderosa atividade antimicrobiana contra fungos, bactérias Gram-positivas e Gram-negativas tais como Aeromonas hydrophila, Enterococcus faecalis, Clostridium botulinum, Staphylococcus aureus, Escherichia coli O157: H7 e Salmonella enterica serovar Typhimurium. A atividade anti-inflamatória induz a apoptose e inibi a proliferação celular, nas respostas imunes mediadas por monócitos/macrófagos, além de diminuir a produção de óxido nítrico induzido por lipopolissacáridos de um modo dependente da dose. CND ao ser administrado por via oral em ratos diabéticos demonstrou melhora no conteúdo de glicogênio muscular e hepático aumentando a liberação de insulina. Além disso CND estimulou a angiogênese in vivo e in vitro, regulando positivamente o fator de crescimento endotelial vascular. Conclusão: A partir do que foi relatado constatou-se que o CND possui muitas atividades com potencial farmacológico, mas percebe-se que se faz necessário estudos sobre o(s) mecanismos(s) de ação dessas atividades a fim de se proporcionar o uso seguro e eficaz do cinamaldeído.

scholarly journals KEGUNAAN EKSTRAK DAUN MENIRAN (Phylanthus niruri) BAGI PENGENDALIAN PENYAKIT IKAN AKIBAT INFEKSI BAKTERI Aeromonas hydrophila

2009 ◽  
Vol 4 (1) ◽  
pp. 54
Author(s):  
Hambali Supriyadi ◽  
Dein Iftitah
Keyword(s):  
Aeromonas Hydrophila ◽  
Phyllanthus Niruri

Penyakit ikan merupakan salah satu masalah serius yang selalu dihadapi oleh para pembudi daya ikan. Penyakit akibat infeksi bakteri terutama yang disebabkan oleh Aeromonas hydrophila telah menyebabkan kerugian yang tidak sedikit. Penanggulangan penyakit infeksi bakteri dengan menggunakan antibiotika telah banyak dilakukan. Namun cara seperti ini apabila dilakukan dengan tidak hati-hati akan menimbulkan banyak masalah, di antaranya adalah terbentuknya bakteri yang resisten dan residunya dalam daging ikan. Cara lain yang dipertimbangkan lebih aman adalah dengan cara pencegahan yaitu dengan penggunaan vaksin dan bahan lain yang dapat menimbulkan kekebalan tubuh. Tanaman meniran (Phyllanthus niruri L.) telah banyak digunakan sebagai obat baik pada manusia maupun pada binatang ternak. Dalam tulisan ini akan dibahas tentang efektivitas ekstrak tanaman meniran terhadap bakteri Aeromonas hydrophila baik secara in-vitro maupun secara in-vivo yang diaplikasikan bagi pengobatan terhadap ikan yang terinfeksi bakteri Aeromonas hydrophila.

Interaction of a novel Bacillus velezensis (BvL03) against Aeromonas hydrophila in vitro and in vivo in grass carp

2019 ◽  
Vol 103 (21-22) ◽  
pp. 8987-8999 ◽  
Author(s):  
Lina Cao ◽  
Lifei Pan ◽  
Liang Gong ◽  
Yahui Yang ◽  
Haocheng He ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Grass Carp ◽  
Bacillus Velezensis

scholarly journals T6SS and ExoA of flesh-eating Aeromonas hydrophila in peritonitis and necrotizing fasciitis during mono- and polymicrobial infections

2019 ◽  
Vol 116 (48) ◽  
pp. 24084-24092 ◽  
Author(s):  
Ana Fernández-Bravo ◽  
Paul B. Kilgore ◽  
Jourdan A. Andersson ◽  
Elizabeth Blears ◽  
Maria José Figueras ◽  
...  
Keyword(s):  
Injection Site ◽  
Necrotizing Fasciitis ◽  
Mixed Infection ◽  
Aeromonas Hydrophila ◽  
Human Case ◽  
Unique Type ◽  
Macrophage Phagocytosis ◽  
Polymicrobial Infections

An earlier report described a human case of necrotizing fasciitis (NF) caused by mixed infection with 4 Aeromonas hydrophila strains (NF1–NF4). While the NF2, NF3, and NF4 strains were clonal and possessed exotoxin A (ExoA), the NF1 strain was determined to be phylogenetically distinct, harboring a unique type 6 secretion system (T6SS) effector (TseC). During NF1 and NF2 mixed infection, only NF1 disseminated, while NF2 was rapidly killed by a contact-dependent mechanism and macrophage phagocytosis, as was demonstrated by using in vitro models. To confirm these findings, we developed 2 NF1 mutants (NF1ΔtseC and NF1ΔvasK); vasK encodes an essential T6SS structural component. NF1 VasK and TseC were proven to be involved in contact-dependent killing of NF2 in vitro, as well as in its elimination at the intramuscular injection site in vivo during mixed infection, with overall reduced mouse mortality. ExoA was shown to have an important role in NF by both NF1-exoA (with cis exoA) and NF2 during monomicrobial infection. However, the contribution of ExoA was more important for NF2 than NF1 in the murine peritonitis model. The NF2∆exoA mutant did not significantly alter animal mortality or NF1 dissemination during mixed infection in the NF model, suggesting that the ExoA activity was significant at the injection site. Immunization of mice to ExoA protected animals from NF2 monomicrobial challenge, but not from polymicrobial infection because of NF2 clearance. This study clarified the roles of T6SS and ExoA in pathogenesis caused by A. hydrophila NF strains in both mouse peritonitis and NF models in monomicrobial and polymicrobial infections.

scholarly journals Enterococcus, Myroides Y Exiguobacterium: GÉNEROS BACTERIANOS CON POTENCIAL PROBIÓTICO PARA EL CULTIVO DE TILAPIA NILÓTICA (Oreochromis niloticus)

2017 ◽  
Vol 22 (3) ◽  
pp. 331-339 ◽  
Author(s):  
Luisa Marcela Villamil Díaz ◽  
Daniel Esguerra Rodríguez
Keyword(s):  
16S Rrna ◽  
Enterococcus Faecalis ◽  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Edwardsiella Tarda ◽  
Tilapia Nilotica ◽  
Myroides Odoratimimus

Se aislaron 120 morfotipos bacterianos de intestino de tilapia y se seleccionaron según su actividad antibacteriana contra patógenos como Aeromonas hydrophila, Streptococcus agalactiae y Edwardsiella tarda, su capacidad de adherencia a mucus intestinal y cinética de crecimiento. Las bacterias seleccionadas se identificaron mediante secuenciación de 16S rRNA y se identificaron como Exigobacterium sp. I9, Enterococcus faecalis I15 y Myroides odoratimimus I19. Además, se evaluó su efecto in vivo sobre el crecimiento de los peces, mediante su adición al alimento de juveniles de Oreochromis niloticus (106 UFC / g, por 15 días). Se determinó la supervivencia luego de un desafío experimental con Edwardsiella tarda por inyección intraperitoneal (100 µL 105 UFC / mL). Las tres bacterias seleccionadas incrementaron la tasa de crecimiento específica, redujeron la mortalidad de los peces durante el desafío experimental con E. tarda y no causaron mortalidad durante la adición en el alimento. Los efectos positivos in vivo se relacionan posiblemente con actividad in vitro; sin embargo, por motivos de bioseguridad se recomienda efectuar estudios posteriores a Exigobacterium sp. I9y E. faecalis I15 dado que se han reportado miembros de este género como causantes de mortalidad en peces, mientras que en el caso de M. odoratimimus I19, es necesario efectuar futuros estudios para verificar su actividad positiva a mayor escala productiva.

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