Multifunctional Tannic Acid/Silver Nanoparticle-Based Mucoadhesive Hydrogel for Improved Local Treatment of HSV Infection: In Vitro and In Vivo Studies

As a phenolic acid, tannic acid can be classified into a polyphenolic group. It has been widely studied in the biomedical field of science because it presents unique antiviral as well as antibacterial properties. Tannic acid has been reported to present the activity against Influeneza A virus, Papilloma viruses, noroviruses, Herpes simplex virus type 1 and 2, and human immunodeficiency virus (HIV) as well as activity against both Gram-positive and Gram-negative bacteria as Staphylococcus aureus, Escherichia coli, Streptococcus pyogenes, Enterococcus faecalis, Pseudomonas aeruginosa, Yersinia enterocolitica, Listeria innocua. Nowadays, compounds of natural origin constitute fundaments of material science, and the trend is called “from nature to nature”. Although biopolymers have found a broad range of applications in biomedical sciences, they do not present anti-microbial activity, and their physicochemical properties are rather poor. Biopolymers, however, may be modified with organic and inorganic additives which enhance their properties. Tannic acid, like phenolic acid, is classified into a polyphenolic group and can be isolated from natural sources, e.g., a pure compound or a component of a plant extract. Numerous studies have been carried out over the application of tannic acid as an additive to biopolymer materials due to its unique properties. On the one hand, it shows antimicrobial and antiviral activity, while on the other hand, it reveals promising biological properties, i.e., enhances the cell proliferation, tissue regeneration and wound healing processes. Tannic acid is added to different biopolymers, collagen and polysaccharides as chitosan, agarose and starch. Its activity has been proven by the determination of physicochemical properties, as well as the performance of in vitro and in vivo studies. This systematics review is a summary of current studies on tannic acid properties. It presents tannic acid as an excellent natural compound which can be used to eliminate pathogenic factors as well as a revision of current studies on tannic acid composed with biopolymers and active properties of the resulting complexes.

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Silver nanoparticle fate in mammals: Bridging in vitro and in vivo studies

Coordination Chemistry Reviews ◽

10.1016/j.ccr.2018.03.008 ◽

2018 ◽

Vol 364 ◽

pp. 118-136 ◽

Cited By ~ 24

Author(s):

Marianne Marchioni ◽

Pierre-Henri Jouneau ◽

Mireille Chevallet ◽

Isabelle Michaud-Soret ◽

Aurélien Deniaud

Keyword(s):

Silver Nanoparticle ◽

In Vivo Studies

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An in vitro study on the ability of tannic acid to inhibit methanogenesis and biohydrogenation of C18 PUFA in the rumen of goats

Annals of Animal Science ◽

10.1515/aoas-2016-0059 ◽

2017 ◽

Vol 17 (2) ◽

pp. 491-502 ◽

Cited By ~ 3

Author(s):

Wisam S. Al-Jumaili ◽

Yong M. Goh ◽

Saied Jafari ◽

Mohamed A. Rajion ◽

Mohamed F. Jahromi ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Tannic Acid ◽

Rumen Fluid ◽

Rumen Fermentation ◽

Gas Production ◽

In Vivo Studies ◽

Negative Effect

Abstract An in vitro gas production technique, using rumen fluid from four Kacang × Boer crossbred adult goats was used to study the effects of commercial tannic acid (TA, a hydrolysable tannin) on methanogenesis, fatty acid composition and biohydrogenation (BH) of C18 polyunsaturated fatty acids (PUFA) in the rumen. Treatments were control (CON, 50% alfalfa hay (AH) + 50% concentrate), 25 mg TA/250 mgDM (LTA, low TA) and 50 mg TA/250 mgDM (HTA, High TA), which were mixed with 30 mL of buffered rumen fluid and incubated for 24 h. The study revealed that TA supplementation had no negative effect on rumen fermentation parameters such as pH, NH3N, acetic/propionic ratio and total volatile fatty acid (tVFA). Methane (CH4) production (mL/250 mg DM) decreased (P<0.05) with increasing levels of TA. Greatest CH4 reduction (%) was recorded for MTA (20.30%) and LTA (13.00%) compared with CON. Supplementation of the diet with TA did not affect the rate of rumen BH (%) of C18:1n-9 (oleic acid; OA), C18:2n-6 (linoleic acid; LA), C18:3n-3 (linolenic acid; LNA) and the concentration of fatty acids after 24 h of in vitro incubation. Based on this study, the addition of TA in vitro reduced rumen methanogenesis without negative effect of rumen fermentation characteristics, but in vivo studies need to be performed to determine if concentrations that inhibit methane are below toxic levels.

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Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels

Scientific Reports ◽

10.1038/s41598-021-01262-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Sakkarin Bhubhanil ◽

Chanon Talodthaisong ◽

Mattaka Khongkow ◽

Katawut Namdee ◽

Prapimpun Wongchitrat ◽

...

Keyword(s):

Wound Healing ◽

Silver Nanoparticle ◽

Guar Gum ◽

Antibacterial Properties ◽

Dermal Fibroblasts ◽

In Vivo Studies ◽

Great Promise ◽

Hydrogel Composite

AbstractBiocompatible materials that act as scaffolds for regenerative medicine are of enormous interest. Hydrogel-nanoparticle composites have great potential in this regard, however evaluations of their wound healing and safety in vivo in animal studies are scarce. Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties. We show that the curcumin-bound silver nanoparticles themselves exhibit low cytotoxicity and enhance proliferation, migration, and collagen production in in vitro studies of human dermal fibroblasts. We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels. Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways. As such these hydrogel composites show great promise as wound dressing materials with high antibacterial capacity.

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Regulation of vascular endothelial growth factor expression in human endometrium by steroids and hypoxia: In vitro and in vivo studies

Journal of the Society for Gynecologic Investigation ◽

10.1016/s1071-5576(97)86195-3 ◽

1998 ◽

Vol 5 (1) ◽

pp. 69A-69A

Author(s):

A SHARKEY ◽

D CHARNOCKJONES ◽

K DAY ◽

H SOWTER ◽

L SVENSSON ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Human Endometrium ◽

In Vivo Studies ◽

Vascular Endothelial ◽

Factor Expression ◽

Growth Factor Expression ◽

Endothelial Growth Factor

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In vitro and in vivo studies of new cationic Zn(II)‐benzonaphthoporphyrazines as photosensitizers for PDT

Journal of Porphyrins and Phthalocyanines ◽

10.1002/jpp.373.abs ◽

2001 ◽

Vol 5 (8) ◽

pp. 645-651

Author(s):

M. Peeva ◽

M. Shopova ◽

U. Michelsen ◽

D. Wöhrle ◽

G. Petrov ◽

...

Keyword(s):

In Vivo Studies

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Effect of caffeine on theophyliine on cerebral blood flow response to brain activation: In vitro and in vivo studies

Journal of Cerebral Blood Flow & Metabolism ◽

10.1038/sj.jcbfm.9591524.0198 ◽

2005 ◽

Vol 25 (1_suppl) ◽

pp. S198-S198

Author(s):

Joseph R Meno ◽

Thien-son K Nguyen ◽

Elise M Jensen ◽

G Alexander West ◽

Leonid Groysman ◽

...

Keyword(s):

Blood Flow ◽

Cerebral Blood Flow ◽

Brain Activation ◽

In Vivo Studies ◽

Blood Flow Response ◽

Flow Response

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Effects of Unfractionated and Low Molecular Weight Heparins on Platelet Thromboxane Biosynthesis “In Vivo”

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648988 ◽

1994 ◽

Vol 72 (06) ◽

pp. 942-946 ◽

Cited By ~ 20

Author(s):

Raffaele Landolfi ◽

Erica De Candia ◽

Bianca Rocca ◽

Giovanni Ciabattoni ◽

Armando Antinori ◽

...

Keyword(s):

Molecular Weight ◽

Unfractionated Heparin ◽

Low Molecular Weight Heparin ◽

Primary Source ◽

In Vivo Studies ◽

Low Molecular Weight ◽

Heparin Administration ◽

To Receive

SummarySeveral “in vitro” and “in vivo” studies indicate that heparin administration may affect platelet function. In this study we investigated the effects of prophylactic heparin on thromboxane (Tx)A2 biosynthesis “in vivo”, as assessed by the urinary excretion of major enzymatic metabolites 11-dehydro-TxB2 and 2,3-dinor-TxB2. Twenty-four patients who were candidates for cholecystectomy because of uncomplicated lithiasis were randomly assigned to receive placebo, unfractionated heparin, low molecular weight heparin or unfractionaed heparin plus 100 mg aspirin. Measurements of daily excretion of Tx metabolites were performed before and during the treatment. In the groups assigned to placebo and to low molecular weight heparin there was no statistically significant modification of Tx metabolite excretion while patients receiving unfractionated heparin had a significant increase of both metabolites (11-dehydro-TxB2: 3844 ± 1388 vs 2092 ±777, p <0.05; 2,3-dinor-TxB2: 2737 ± 808 vs 1535 ± 771 pg/mg creatinine, p <0.05). In patients randomized to receive low-dose aspirin plus unfractionated heparin the excretion of the two metabolites was largely suppressed thus suggesting that platelets are the primary source of enhanced thromboxane biosynthesis associated with heparin administration. These data indicate that unfractionated heparin causes platelet activation “in vivo” and suggest that the use of low molecular weight heparin may avoid this complication.

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