scholarly journals Molecular Characterization and Genetic Diversity of Yellow Passion Fruit Based on RAPD Markers

2019 ◽  
Vol 11 (3) ◽  
pp. 575
Author(s):  
Angélica Vieira Sousa Campos ◽  
José Ricardo Peixoto ◽  
Fábio Gelape Faleiro ◽  
Michelle Souza Vilela ◽  
Márcio de Carvalho Pires
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Binary Data ◽  
Rapd Markers ◽  
Molecular Genetic ◽  
Passion Fruit ◽  
Passiflora Edulis ◽  
The University ◽  
Yellow Passion Fruit

Molecular genetic variability studies are essential to complement the agronomic characterization of yellow passion fruit genotypes (Passiflora edulis Sims). Therefore, this study aimed at evaluating the genetic diversity of 24 genotypes of yellow passion fruit obtained from a research program developed by the University of Brasilia and Embrapa Cerrados, using RAPD molecular markers. RAPD markers were obtained from eight decamer primers and converted into a matrix of binary data, from which genetic dissimilarities among genotypes were estimated, and clustering analysis was performed. A total of 54 RAPD markers were obtained, with 6.8 bands per primer on average. From this total, 46 (85.2%) RAPD markers were polymorphic. The OPD10 primer presented the highest number of polymorphic bands. The high percentage of polymorphic markers evidenced the existence of genetic variability among genotypes. Nei’s genetic distance between genotypes ranged from 0.043 to 0.451. Clustering resulted in the formation of at least five groups of similarity.

Development and characterization of microsatellite markers from the yellow passion fruit (Passiflora edulis f. flavicarpa)

2005 ◽  
Vol 5 (2) ◽  
pp. 331-333 ◽  
Author(s):  
E. J. OLIVEIRA ◽  
J. G. PÁDUA ◽  
M. I. ZUCCHI ◽  
L. E. A. CAMARGO ◽  
M. H. P. FUNGARO ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Passion Fruit ◽  
Passiflora Edulis ◽  
Yellow Passion Fruit

scholarly journals Lipid peroxidation and seed emergency in progenies of the yellow passion fruit plant

2012 ◽  
Vol 34 (3) ◽  
pp. 711-718 ◽  
Author(s):  
João Paulo Bestete de Oliveira ◽  
Rodrigo Sobreira Alexandre ◽  
Jacson Rondinelli da Silva Negreiros ◽  
José Carlos Lopes ◽  
Claudio Horst Bruckner
Keyword(s):  
Lipid Peroxidation ◽  
Experimental Design ◽  
Genetic Variability ◽  
Passion Fruit ◽  
Passiflora Edulis ◽  
Mda Content ◽  
Passiflora Edulis Sims ◽  
Yellow Passion Fruit

The objective was to evaluate the percentage of emergency plantlets and lipid peroxidation in seeds of 29 half-sib progenies of yellow passion fruit (Passiflora edulis Sims.) after 24 months under storage. The experimental design was completely randomized, with four replications of 50 seeds each, from which the treatments were the progenies (1-29). The evaluation of the percent plantlet emergency was accomplished at 14 and 28 days after sowing. The lipid peroxidation of the seeds was expressed as malondialdehyde (MDA) content that was determined by the TBARS method. Approximately 21% of those half-sib progenies maintained the viability of their seeds for twenty-four months under storage. The results point out a remarkable genetic variability for vigor and emergency of the yellow passion fruit plantlets, with occurrence of individuals with high and other ones with low capacity to maintaining the physiologic quality of their seeds after storage.

scholarly journals Genetic diversity in yellow passion fruit (Passiflora edulis Sims) based on RAPD

2010 ◽  
Vol 10 (2) ◽  
pp. 154-159 ◽  
Author(s):  
C.B.M. Cerqueira-Silva ◽  
L.D.H.C.S. Conceição ◽  
C.B. Cardoso-Silva ◽  
A.S. Pereira ◽  
E.S.L. Santos ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Passion Fruit ◽  
Passiflora Edulis ◽  
Passiflora Edulis Sims ◽  
Yellow Passion Fruit

scholarly journals Genetic diversity characterization of cassava cultivars (Manihot esculenta Crantz).: I) RAPD markers

1998 ◽  
Vol 21 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Carlos Colombo ◽  
Gérard Second ◽  
Tereza Losada Valle ◽  
André Charrier
Keyword(s):  
Genetic Diversity ◽  
Multivariate Analysis ◽  
Genetic Variability ◽  
Manihot Esculenta ◽  
Core Collection ◽  
Rapd Markers ◽  
Manihot Esculenta Crantz ◽  
Important Resource ◽  
Flour Production

RAPD markers were used to investigate the genetic diversity of 31 Brazilian cassava clones. The results were compared with the genetic diversity revealed by botanical descriptors. Both sets of variates revealed identical relationships among the cultivars. Multivariate analysis of genetic similarities placed genotypes destinated for consumption "in nature" in one group, and cultivars useful for flour production in another. Brazil’s abundance of landraces presents a broad dispersion and is consequently an important resource of genetic variability. The botanical descriptors were not able to differentiate thirteen pairs of cultivars compared two-by-two, while only one was not differentiated by RAPD markers. These results showed the power of RAPD markers over botanical descriptors in studying genetic diversity, identifying duplicates, as well as validating, or improving a core collection. The latter is particularly important in this vegetatively propagated crop.

scholarly journals Molecular characterization of elephantgrass accessions through RAPD markers

2005 ◽  
Vol 29 (3) ◽  
pp. 568-574 ◽  
Author(s):  
Leônidas Paixão Passos ◽  
Marco Antonio Machado ◽  
Maria Coletta Vidigal ◽  
Ana Lúcia Campos
Keyword(s):  
Genetic Variability ◽  
Genetic Distance ◽  
Binary Data ◽  
Rapd Markers ◽  
Dna Amplification ◽  
Distance Matrix ◽  
Genetic Distances ◽  
Average Genetic Distance ◽  
Genetic Distance Matrix

Elephantgrass pastures are limited by yield variations and reductions in forage quality and availability, thus making the search for genotypes with reduced seasonality a major concern. In order to verify the extent of genetic variability among contrasting cultivars, ten elephantgrass accessions were analyzed through DNA amplification by RAPD technique. A total of 160 DNA bands were generated with the use of 44 random primers and 23% of these bands were monomorphic for all accessions. Gel-obtained binary data (1 for presence and 0 for absence) were used for generating a genetic distance matrix, which was utilized in a UPGMA grouping analysis. Elephantgrass cultivars Cameroon and Vruckwona were the accessions mostly divergent from the others, with an average genetic distance of 0.34. The accessions with the lowest average genetic distances from the others were Pioneiro and CNPGL 27-5, both with a distance of 0.25. Overall, genetic distances ranged from 0.06 to 0.43, indicating little genetic variability for the set of accessions, despite the contrasting morphology of the studied genotypes.

scholarly journals Effect of selection on genetic variability in yellow passion fruit

2012 ◽  
Vol 12 (4) ◽  
pp. 253-260 ◽  
Author(s):  
Juliana Leles Costa ◽  
Onildo Nunes de Jesus ◽  
Gilmara Alvarenga Fachardo Oliveira ◽  
Eder Jorge de Oliveira
Keyword(s):  
Genetic Variability ◽  
Principal Component ◽  
Inter Simple Sequence Repeat ◽  
Passion Fruit ◽  
Neighbor Joining ◽  
Molecular Polymorphism ◽  
Passiflora Edulis ◽  
Simple Sequence ◽  
Genotype A ◽  
Yellow Passion Fruit

The objective of this study was to evaluate the genetic diversity in improved (IG) and unimproved germplasm (UIG) of yellow passion fruit (Passiflora edulis Sims f. flavicarpa Deg), based on ISSR (Inter Simple Sequence Repeat) markers. The genotypes were grouped by neighbor joining clustering and principal component analysis. Regardless of the genotype, a high number of polymorphic bands was observed, aside from several specific fragments of the groups, according to the level of improvement. Unimproved genotypes had a higher number of polymorphic fragments. The hierarchical and principal component clustering coincided in the formation of two rather distinct groups (IG and UIG). The molecular analysis of variance to check the differentiation between groups showed 57 and 43% of the variation within and between groups, respectively. This study demonstrated the potential of ISSR to determine molecular polymorphism in yellow passion fruit and that breeding has narrowed the genetic variability.

scholarly journals Molecular characterization of oats (Avena sativa L.) germplasm with microsatellite markers

2021 ◽  
Vol 81 (01) ◽  
pp. 144-147
Author(s):  
Gagandeep Kaur ◽  
Rahul Kapoor ◽  
Priti Sharma ◽  
Puja Srivastava
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Avena Sativa ◽  
Symmetric Matrix ◽  
Molecular Genetic ◽  
Microsatellite Primers ◽  
Dice Coefficient ◽  
Moderate Amount ◽  
Geographical Regions

Assessment of genetic diversity between selected genotypes serves as an essential tool to exploit the genetic variability. In the present study, Ninety nine accessions of oat acquired from different geographical regions were used to study the molecular genetic variability by the use of 91 microsatellite primers. A total of 248 alleles from 91 primer pairs in the 99 oat genotypes were detected. The average alleles per primer pair were recorded as 2.69. The primer pairs having the highest PIC values viz., AM 6 (0.50), AM 55 (0.49) and M 83381 (0.49) can be further used in the association mapping studies. Clustering of the oat genotypes into two main clusters A and B was done by using the symmetric matrix of DICE coefficient based on UPGMA method in Software – DARwin 6.0. In this study, the moderate amount of dissimilarity was observed between the whole set of accessions, the values ranging from 0.33 to 0.79. The individuals belonging to the different clusters may be useful in hybridization studies of oat improvement programmes.

scholarly journals Genetic Diversity of Purple Passion Fruit, Passiflora edulis f. edulis, Based on Single-Nucleotide Polymorphism Markers Discovered through Genotyping by Sequencing

Diversity ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 144
Author(s):  
Nohra Castillo Rodríguez ◽  
Xingbo Wu ◽  
María Isabel Chacón ◽  
Luz Marina Melgarejo ◽  
Matthew Wohlgemuth Blair
Keyword(s):  
Genetic Diversity ◽  
Single Nucleotide Polymorphism ◽  
Genotyping By Sequencing ◽  
Passion Fruit ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Passiflora Edulis ◽  
Tropical Fruit ◽  
Commercial Cultivars ◽  
Purple Passion Fruit

Orphan crops, which include many of the tropical fruit species used in the juice industry, lack genomic resources and breeding efforts. Typical of this dilemma is the lack of commercial cultivars of purple passion fruit, Passiflora edulis f. edulis, and of information on the genetic resources of its substantial semiwild gene pool. In this study, we develop single-nucleotide polymorphism (SNP) markers for the species and show that the genetic diversity of this fruit crop has been reduced because of selection for cultivated genotypes compared to the semiwild landraces in its center of diversity. A specific objective of the present study was to determine the genetic diversity of cultivars, genebank accession, and landraces through genotyping by sequencing (GBS) and to conduct molecular evaluation of a broad collection for the species P. edulis from a source country, Colombia. We included control genotypes of yellow passion fruit, P. edulis f. flavicarpa. The goal was to evaluate differences between fruit types and compare landraces and genebank accessions from in situ accessions collected from farmers. In total, 3820 SNPs were identified as informative for this diversity study. However, the majority distinguished yellow and purple passion fruit, with 966 SNPs useful in purple passion fruits alone. In the population structure analysis, purple passion fruits were very distinct from the yellow ones. The results for purple passion fruits alone showed reduced diversity for the commercial cultivars while highlighting the higher diversity found among landraces from wild or semi-wild conditions. These landraces had higher heterozygosity, polymorphism, and overall genetic diversity. The implications for genetics and breeding as well as evolution and ecology of purple passion fruits based on the extant landrace diversity are discussed with consideration of manual or pollinator-assisted hybridization of this species.

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