scholarly journals Identification and Characterization of Bacterial Agents Causing Moderate Damage and Souring of the Fig Fruits

2021 ◽  
Vol 10 (1) ◽  
pp. 40
Author(s):  
Seyedeh Asiyeh Mousavi ◽  
Nader Hasanzadeh
Keyword(s):  
Pathogenic Bacteria ◽  
Fruit Rot ◽  
Phenotypic Traits ◽  
Rrna Gene ◽  
Pseudomonas Fragi ◽  
Disease Symptoms ◽  
Bacillus Paralicheniformis ◽  
Fig Trees ◽  
Identification And Characterization

In order to determine the factors of decay and sourness of fig fruits, in the summer of 2016-17, 60 leaf, fruit and stem samples from different regions of Tehran, Varamin (Qal'e No), Mazandaran (Amol, Noor and Sari), Lorestan from Iran and a branch of fig fruit sample from Italy were collected. We obtained 30 isolates from the sample. The pathogenicity of 30 isolates were confirmed by artificially inoculation using fig fruits. They were also characterized based on key phenotypic traits. All 30 isolates showed hypersensitivity reaction to tobacco, pelargonium and did not show pathogenicity to potato tubers. 16S rRNA gene of the 10 representative isolates were sequenced. Ten isolates were identified as Stenotrophomonas maltophilia, Pseudomonas aeruginosa, Pseudomonas fulva, Brevibacterium linens, Pseudomonas fragi, Bacillus licheniformis, Bacillus paralicheniformis and Bacillus cereus based on the determined sequences. None of the isolates caused fruit rot but typical disease symptoms were observed on fig leaves and fruits. This is the first report of the presence of pathogenic bacteria on fig trees in Iran.

Identification and Characterization of Enterococcus spp. in Greek Spontaneous Sausage Fermentation

2008 ◽  
Vol 71 (6) ◽  
pp. 1244-1247 ◽  
Author(s):  
SPIROS PARAMITHIOTIS ◽  
DAFNI-MARIA KAGKLI ◽  
VASILIKI A. BLANA ◽  
GEORGE-JOHN E. NYCHAS ◽  
ELEFTHERIOS H. DROSINOS
Keyword(s):  
Phenotypic Traits ◽  
Rrna Gene ◽  
Strain Typing ◽  
Phenotypic Properties ◽  
Fermented Sausages ◽  
Enterococcus Spp ◽  
Identification And Characterization ◽  
The 16S Rrna Gene

A total of 108 enterococcal strains previously isolated from spontaneously fermented sausages were identified using phenotypic traits, pulsed-field gel electrophoresis (PFGE), and sequencing of the 16S rRNA gene. The proteolytic and lipolytic activities of these isolates and their ability to decarboxylate lysine, tyrosine, ornithine, and histidine and to produce antimicrobial compounds also were assessed. All strains were identified as Enterococcus faecium, and a lack of correlation between data derived from phenotypic and those derived from genotypic techniques was evident. Wide strain diversity was revealed by both phenotypic properties and PFGE strain typing results. Few strains were present in all batches, suggesting a possible persistence in the respective production plants. Neither proteolytic nor lipolytic activities were detected, and none of the strains decarboxylated lysine, tyrosine, ornithine, or histidine. A total of 42 E. faecium strains inhibited in vitro growth of Listeria monocytogenes, which suggests possible contribution of these strains to the safety of the end product and possible utilization of these strains as protective cultures.

scholarly journals Identification and Characterization of a Well-Defined Series of Coronatine Biosynthetic Mutants of Pseudomonas syringae pv. tomato DC3000

2004 ◽  
Vol 17 (2) ◽  
pp. 162-174 ◽  
Author(s):  
David M. Brooks ◽  
Gustavo Hernández-Guzmán ◽  
Andrew P. Kloek ◽  
Francisco Alarcón-Chaidez ◽  
Aswathy Sreedharan ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
High Performance ◽  
Tomato Dc3000 ◽  
Disease Symptoms ◽  
Coronafacic Acid ◽  
Tn5 Mutants ◽  
Cor Genes ◽  
Identification And Characterization

To identify Pseudomonas syringae pv. tomato genes involved in pathogenesis, we carried out a screen for Tn5 mutants of P. syringae pv. tomato DC3000 with reduced virulence on Arabidopsis thaliana. Several mutants defining both known and novel virulence loci were identified. Six mutants contained insertions in biosynthetic genes for the phytotoxin coronatine (COR). The P. syringae pv. tomato DC3000 COR genes are chromosomally encoded and are arranged in two separate clusters, which encode enzymes responsible for the synthesis of coronafacic acid (CFA) or coronamic acid (CMA), the two defined intermediates in COR biosynthesis. High-performance liquid chromatography fractionation and exogenous feeding studies confirmed that Tn5 insertions in the cfa and cma genes disrupt CFA and CMA biosynthesis, respectively. All six COR biosynthetic mutants were significantly impaired in their ability to multiply to high levels and to elicit disease symptoms on A. thaliana plants. To assess the relative contributions of CFA, CMA, and COR in virulence, we constructed and characterized cfa6 cmaA double mutant strains. These exhibited virulence phenotypes on A. thalliana identical to those observed for the cmaA or cfa6 single mutants, suggesting that reduced virulence of these mutants on A. thaliana is caused by the absence of the intact COR toxin. This is the first study to use biochemically and genetically defined COR mutants to address the role of COR in pathogenesis.

scholarly journals Identification and characterization of the fungus Dothiorella sarmentorum on necrotic shoots of declining ash in Slovakia

2018 ◽  
Vol 45 (1) ◽  
pp. 53-57
Author(s):  
Helena Ivanová
Keyword(s):  
Abiotic Factors ◽  
Seed Orchard ◽  
Economic Consequences ◽  
Cumulative Effects ◽  
Disease Symptoms ◽  
Hymenoscyphus Fraxineus ◽  
Ash Trees ◽  
Branch Dieback ◽  
Identification And Characterization

Abstract Formerly, before the current Hymenoscyphus fraxineus epidemic, symptomatic ash twigs were habitually colonized by fungi occurring occasionally in the early stages of ash diseases. Some of these fungi are endophytes or facultative parasites. The segments of diseased shoots of the studied trees were collected from a seed orchard situated in southwest Slovakia. The frequently isolated fungus Dothiorella sarmentorum was identified microscopically and characterized with the aid of morphological keys. The disease symptoms comprise wood cankers, bud necrosis, and shoot and branch dieback displayed through bleached, necrotic or discoloured canes in infected trees. Despite the Dothiorella species has not yet been recognized to have significant economic consequences, the cumulative effects of various stressful biotic and abiotic factors may induce disorder and decline of ash trees in Slovakia.

scholarly journals Identification and characterization of Flavobacteriaceae from farmed Oreochromis niloticus and Clarius gariepinus in Uganda

2018 ◽  
Vol 8 (1) ◽  
pp. 42-50
Author(s):  
AMONO RACHEAL ◽  
CHRISTOPHER J. KASANGA ◽  
DENIS K. BYARUGABA
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Oreochromis Niloticus ◽  
Control Measure ◽  
African Catfish ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Identification And Characterization

Racheal A, Kasanga CJ, Byarugaba DK. 2018. Identification and characterization of Flavobacteriaceae from farmed Oreochromis niloticusand Clarius garieoinus. Bonorowo Wetlands 2: 42-50. Bacteria under family Flavobacteriaceae (in this study were also referred to as Flavobacteria) are important pathogens of fish, people, many other animals and plants. In this study, Flavobacteria from Nile tilapia (Oreochromis niloticus) and African catfish (Clarius gariepinus) were identified and characterized from the selected farms in Uganda. Gill and skin swabs were obtained from a total of 119 fish from 19 farms and were dissected aseptically to sample internal organs. The samples were inoculated onto Sheih media and incubated at 25°C for 48 hours. The suspected isolates were identified by colon characteristics, conventional biochemical tests and API 20 NE kits. The isolates were grouped into eight based on colon characteristic similarity. One isolate was selected per group for 16S rRNA gene sequencing and identified using the EZbiocloud.net ID software. Phylogenetic analysis of selected isolates was performed using the 16S rRNA gene sequences in BioEdit and MEGA 7.0.2 software. Basing on extrapolation of sequence analysis of the selected isolates, out of the 86 isolates, Myroides marinus was the most predominant species taking up 4 of the 8 groups (60 isolates) in 13 farms. The rest of the groups comprised of; Acinetobacter pitti, one group (6 isolates) in 6 farms, Chryseobacterium gambrini 2 groups (3 isolates) in 3 farms and one isolate was unidentified, in 3 farms. However, a total of 16 isolates did not grow on sub culturing. Phylogenetic analysis indicated that M. marinus isolates grouped with other M. marinus isolates from gene bank with significant intra-species diversity which was also observed with C. gambrini isolates. All the sampled farms had at least one isolate of a Flavobacterium from Tilapia and/or Catfish. Pathogenicity studies should be conducted on the isolates to establish their importance as fish pathogens and transmission dynamics so that an appropriate control measure can be recommended.

scholarly journals Isolation and Characterization of Endophytic Bacteria from Tembelekan (Lantana camara L.) as Antibacterial Compounds Producer

2015 ◽  
Vol 2 (2) ◽  
pp. 86-98
Author(s):  
Dina Dyah Saputri ◽  
Maria Bintang ◽  
Fachriyan H Pasaribu
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Endophytic Bacteria ◽  
Pathogenic Bacteria ◽  
Lantana Camara ◽  
Rrna Gene ◽  
Antibacterial Compounds ◽  
Isolation And Characterization ◽  
Ms Analysis

Endophytic bacteria are microorganisms that live in the internal tissues of plants and have symbiotic mutualism with their host plants. Endophytic bacteria may produce secondary metabolites that can be developed for medical, agricultural, and industrial purposes. Lantana camara is a medicinal plant that has therapeutic potential to treat a variety of diseases such as fever, tuberculosis, rheumatism, asthma, and skin disease. The purpose of this study was to isolate and characterize endophytic bacteria from Lantana camara which has potential to produce antibacterial compounds. The method of this research include isolation of endophytic bacteria of Lantana camara. Antibacterial activity assay was done against four types of pathogenic bacteria i.e. Bacillus cereus, Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis. Characterization of endophytic bacteria was by 16S rRNA gene analysis and identification of antibacterial compounds by GC-MS analysis. Isolation of endophytic bacteria from Lantana camara resulted in BT22 as a potential isolate. Analysis of 16S rRNA gene showed that the BT22 isolate was similar to Bacillus amyloliquefaciens YB-1402 with 99% identity. The results of GC-MS analysis showed some antibacterial compounds such as: Cyclohexanone, 2-[2-(1,3-dithiolan-2-yl)propyl]-6-methyl-3-(1-methylethyl), Octadecane (CAS) n-Octadecane and Tetracosane (CAS) n-Tetracosane.

scholarly journals Identification and Characterization of Pestalotiopsis spp. Causing Scab Disease of Guava, Psidium guajava, in Hawaii

Plant Disease ◽  
2006 ◽  
Vol 90 (1) ◽  
pp. 16-23 ◽  
Author(s):  
Lisa M. Keith ◽  
Maile E. Velasquez ◽  
Francis T. Zee
Keyword(s):  
Fungal Pathogens ◽  
Genetic Resource ◽  
Psidium Guajava ◽  
Molecular Methods ◽  
Fruit Rot ◽  
Tropical Plant ◽  
Characterization Studies ◽  
The Tropics ◽  
Identification And Characterization

Guava is one of the most widely grown plants in the tropics; however, it is affected by many fruit rot diseases. Fruit diseases decrease the marketability of fresh fruit and fruit for processing. A survey of scab disease was conducted at the USDA/ARS Tropical Plant Genetic Resource Management Unit in Hilo, HI, where more than 50 accessions of guava are grown. Symptoms observed were gray/light brown lesions surrounded by dark brown borders on leaves and brown, raised, corky, necrotic lesions on the exocarp of fruit which progressed as the fruits matured. Seventeen isolates from infected fruit, six isolates from lesions on leaves, and nine isolates from additional crops surrounding the guava trees were collected. The main fungi consistently isolated from symptomatic leaves and fruit were Pestalotiopsis spp. Morphology, colony characteristics, and pathogenicity of the isolates were examined and potential sources of host resistance were identified for germplasm characterization studies. Molecular methods were used to identify four Pestalotiopsis taxa (P. clavispora, P. microspora, P. sp. GJ-1, and P. disseminata) on guava in Hawaii. To our knowledge, this is the first report of traditional and molecular methods of identification and characterization being used for fungal pathogens of guava in Hawaii.

Molecular identification of bile tolerant Lactobacillus fermentum strains isolated from Human Faeces

2016 ◽  
Vol 1 (02) ◽  
pp. 182-189
Author(s):  
M. Kumar ◽  
P. K. Sharma ◽  
P. K. Sharma ◽  
N. Kumar
Keyword(s):  
Pathogenic Bacteria ◽  
Molecular Methods ◽  
Lactobacillus Fermentum ◽  
Rrna Gene ◽  
Probiotic Properties ◽  
Phenotypic Characteristics ◽  
Antimicrobial Substances ◽  
Polymerase Chain ◽  
Specific Part

Conventional procedures of identification are laborious and time consuming because they depend on growth of microorganism in that particular environment and hence, sometimes, give misleading results while molecular methods relies upon generating a large number of copies of a specific part of its genome for identification of unknown isolates and enables the problem of poor identification. The identification of a microbial isolate to genus level imparts a partial characterization of the isolate revealing a lot of information about that organism. Lactic acid bacteria are very significant to human health due to their probiotic attributes, production of antimicrobial substances and subsequently to inhibit pathogenic bacteria. Bile tolerance has been one of the major probiotic criterion while selecting the isolate for probiotic properties. This work aims at looking directly at the genome of bile tolerant Lactobacillus isolates using genotypic and phenotypic characteristics. The isolates were identified by sequencing specific region of 16 S r DNA segment, after amplification by PCR, and then comparing the results to sequences from related database. The results from both conventional and molecular methods were then compared. Twenty five isolates (25) of Lactobacillus fermentum were isolated from fecal samples and identified using API- 50 CH. The isolates were further identified using molecular methods using polymerase chain reaction (PCR) for amplification of 16S rRNA gene to confirm their identities. The genotypic characterization however showed that 84% of the organisms identified using conventional method as L. fermentum correlated, while 16% did not correlate; 2 were identified as Lactobacillus planterum, 01 as L. delbrueckiiand 01 as L.paraplanterum.

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