scholarly journals Polysaccharides study of three mushrooms growing in Mongolia

2018 ◽  
pp. 47-52
Author(s):  
Naranmandakh Sh ◽  
V V Golovchenko ◽  
О А Patova ◽  
Degiimaa T ◽  
Soninkhuu Sh
Keyword(s):  
Molecular Mass ◽  
Monosaccharide Composition ◽  
Water Soluble ◽  
Hot Water ◽  
Inonotus Obliquus ◽  
Average Molecular Mass ◽  
Medicinal Mushrooms ◽  
Laetiporus Sulphureus ◽  
Soluble Polysaccharide ◽  
Mushroom Sample

This study was aimed to analyze of polysaccharides from fruiting bodies of Fomitopsis officinalis (Vill.ex.Fr.), Laetipo-rus sulphureus (Bull.ex.Fr.) and Inonotus obliquus (Pers.ex.Fr.) medicinal mushrooms, growing in Mongolia. As a result of “step by step” extraction method we have obtained 4 fractions of polysaccharide (water-soluble: HWE50 and HWE100; cold alkali-soluble CA; hot alkali-soluble HA) form the each mushroom sample. Polysaccharide fraction’s yields extracted by hot water from Fomitopsis officinalis (Vill.ex.Fr.), Laetiporus sulphureus (Bull.ex.Fr.) and Inonotus obliquus (Pers.ex.Fr.) were 3.68%, 8.82% and 12.84%. Yields of alkali-soluble polysaccharides were 18.64%, 68.03% and 28.75%, respectively, which results more than water fractions. The main monosaccharide composition in all polysaccharide fractions were glucose, and a content of glucose was varied 30%-100%. Therefore, they can be considered glucans. Water-soluble polysaccharide fractions contain fucose (until 12%), man-nose (until 18%) and galactose (until 30%). An average molecular mass of water-soluble polysaccharides were calculated Mw 100–150kDa or it can be considered a low, while an average molecular mass of alkali-soluble polysaccharides was a bit high 300–750кDа. All polysaccharides were heterogenic. Монгол орны гурван зүйл мөөгний полисахаридын судалгааны дүн Хураангуй: Монгол оронд ургадаг агил мөөг Fomitopsis officinalis (Vill.ex.Fr.), туруудай мөөг Laetiporus sulphureus (Bull.ex.Fr.) ба чага мөөг Inonotus obliquus (Pers.ex.Fr.) гэсэн гурван зүйлийн эмийн мөөгний полисахаридын найрлагыг судлав. Мөөгийг шат дараалан хандлах аргаар мөөг тус бүрээс 4 төрлийн полисахарид (усанд хандлагддаг: HWE50 ба HWE100; хүйтэн шүлтэнд хандлагддаг CA; халуун шүлтэнд хандлагддаг HA)-ыг ялган авав. Усанд хандлагддаг полисахаридуудын гарц агил Fomitopsis officinalis (Vill.ex.Fr.), туруудай Laetiporus sulphureus (Bull.ex.Fr.) ба чага Inonotus obliquus (Pers.ex.Fr.) мөөг бүрт тус тус 3.68%, 8.82% ба 12.84% байв. Харин шүлтэнд хандлагддаг полисахаридын гарц нь усанд хандлагддаг полисахаридтай харьцуулахад их буюу тус тус 18.64%, 68.03% ба 28.75% байв. Бүх полисахаридын хувьд үндсэн мономер нь глюкоз ба түүний агууламж 30%-100%-д хүрч байв. Иймд тэдгээр нь глюкан полисахаридууд болно. Мөн усанд хандлагддаг полисахаридуудын найрлаганд фукоз (12% хүртэл), манноз (18% хүртэл) галактоз (30% хүртэл) агуулагдаж байв. Усаар хандлагддаг глюканууд нь бага дундаж молекул масстай (Mw 100–150кДа) байсан бол натрийн гидроксидоор хандлагддаг глюкануудын молекул масс 300–750кДа харьцангуй их байв. Бүх глюкан нь гетероген чанартай байна. ЦСР-ын аргын дүнд ялган авсан полисахаридууд ойролцоо бүтэцтэй салбарласан хэлхээтэй [бета]- глюканууд болох нь батлагдав. Түлхүүр үгс: агил, туруудай, чага, полисахаридын бүтэц, дундаж молекулын масс

scholarly journals Characterization and Prebiotic Potential of Longan Juice Obtained by Enzymatic Conversion of Constituent Sucrose into Fructo-Oligosaccharides

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2596 ◽  
Author(s):  
Yongxia Cheng ◽  
Haibo Lan ◽  
Lei Zhao ◽  
Kai Wang ◽  
Zhuoyan Hu
Keyword(s):  
Soluble Sugar ◽  
Monosaccharide Composition ◽  
Water Soluble ◽  
Lactobacillus Delbrueckii ◽  
Soluble Solids ◽  
Enzyme Treatment ◽  
Bacteria Growth ◽  
Before And After ◽  
Soluble Polysaccharide

The prebiotic potential of longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide was investigated. The physicochemical properties and carbohydrate composition of the longan juice was evaluated before and after enzymatic treatment. The stimulation effects of the treated longan juice on probiotic bacteria growth were also studied in vitro. The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment. The water-soluble polysaccharide content, including pectin, was significantly increased. Compared with the natural longan pulp, the enzyme treated juice showed a significant decrease in sucrose content. Substantial fructo-oligosaccharides including 1-kestose and nystose were synthesized after enzyme treatment. The molecular weight distribution and the monosaccharide composition of the water-soluble polysaccharide were significantly changed by enzyme treatment. The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.

scholarly journals Levan-Producing Leuconostoc citreum Strain BD1707 and Its Growth in Tomato Juice Supplemented with Sucrose

2015 ◽  
Vol 82 (5) ◽  
pp. 1383-1390 ◽  
Author(s):  
Jin Han ◽  
Xiaofen Xu ◽  
Caixia Gao ◽  
Zhenmin Liu ◽  
Zhengjun Wu
Keyword(s):  
Molecular Mass ◽  
Sugar Content ◽  
Carbon Sources ◽  
Viable Cell ◽  
Monosaccharide Composition ◽  
Tomato Juice ◽  
Average Molecular Mass ◽  
Content Type ◽  
Leuconostoc Citreum ◽  
Sucrose Medium

ABSTRACTA levan-producing strain, BD1707, was isolated from Tibetan kefir and identified asLeuconostoc citreum. The effects of carbon sources on the growth ofL. citreumBD1707 and levan production in tomato juice were measured. The changes in pH, viable cell count, sugar content, and levan yield in the cultured tomato juice supplemented with 15% (wt/vol) sucrose were also assayed.L. citreumBD1707 could synthesize more than 28 g/liter of levan in the tomato juice-sucrose medium when cultured at 30°C for 96 h. Based on the monosaccharide composition, molecular mass distribution, Fourier transform infrared (FTIR) spectra, and nuclear magnetic resonance (NMR) spectra, the levan synthesized byL. citreumBD1707 was composed of a linear backbone consisting of consecutive β-(2→6) linkedd-fructofuranosyl units, with an estimated average molecular mass of 4.3 × 106Da.

scholarly journals Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Huajie Zhao ◽  
Yufei Lan ◽  
Hui Liu ◽  
Yongfa Zhu ◽  
Weiru Liu ◽  
...  
Keyword(s):  
Functional Foods ◽  
Reducing Power ◽  
Monosaccharide Composition ◽  
Vital Role ◽  
Hot Water ◽  
Composition Analysis ◽  
Laetiporus Sulphureus ◽  
Exploitation And Utilization ◽  
Natural Drugs

In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus. Both H-SMPS and E-SMPS were found to have high reducing power and potential scavenging activities against hydroxyl, DPPH, and superoxide anion radicals. In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation. Furthermore, monosaccharide composition analysis showed that fucose may play a vital role in guaranteeing stronger hepatoprotection. These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.

scholarly journals Research of Inonotus obliquus Oligosaccharide in Prevention of Hyperlipidemia

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Dawei Wu ◽  
Yanrong Zhang ◽  
Dawei Wang ◽  
Tingting Liu ◽  
Shanshan Zhang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Protective Effect ◽  
Intestinal Flora ◽  
Deae Cellulose ◽  
Monosaccharide Composition ◽  
Hot Water ◽  
Molar Ratio ◽  
Inonotus Obliquus ◽  
Glycosidic Bonds ◽  
Liver And Kidney

In this study, hot water was used to extract Inonotus obliquus oligosaccharide. DEAE-cellulose and Sepharose G-200 were used to purify Inonotus obliquus oligosaccharide. Inonotus obliquus oligosaccharide IOP-2A was obtained. Its molecular weight Mw is about 1000 Da. The monosaccharide composition and molar ratio were glucose : xylose : galactose : mannose = 54.1 : 13.6 : 13.2 : 6.7. In addition, it also contains a small amount of galactose, gluconic acid, rhamnose, and fucose. IOP-2A contained mainly β-glycosidic bonds. Among them, 1,4-glycosidic bonds accounted for 9.2%, and 1,6-glycosidic bonds accounted for 85.1%. Oligosaccharide macromolecules formed a layered structure. Mouse experiments showed that IOP-2A had the function of preventing hyperlipidemia. At the same time, IOP-2A had a certain protective effect on the liver and kidney. The mechanism of IOP-2A in preventing hyperlipidemia was obtained from the perspective of mouse intestinal flora.

scholarly journals Optimization of extraction of Chaenomeles lagenaria polysaccharide and its antibacterial activity

BioResources ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. 3394-3407
Author(s):  
Yahua Wu ◽  
Xinyue Yin ◽  
Jie Li ◽  
Shiquan Qian
Keyword(s):  
Antibacterial Activity ◽  
Galacturonic Acid ◽  
Food Industry ◽  
Ethanol Concentration ◽  
Water Soluble ◽  
Hot Water ◽  
Hot Water Extraction ◽  
Natural Preservative ◽  
Soluble Polysaccharide ◽  
And Staphylococcus Aureus

A water-soluble polysaccharide from Chaenomeles lagenaria (CLWSP-1) was purified and structurally identified. Then, response surface methodology (RSM) was performed to optimize the hot water extraction (HWE) of CLWSP-1. In addition, the antibacterial activity of CLWSP-1 was also evaluated. The results indicated that the polysaccharide CLWSP-1 mainly contained galacturonic acid, arabinose, and galactose, and its molecular weight was 1.23 × 102 kDa. The optimal HWE for extraction of CLWSP-1 was a ratio of water to solid of 48.9 mL/g, temperature of 91 °C, and an extraction time of 114 min; an ethanol concentration of 81% and a 1.24% concentration of CLWSP-1 were achieved. Moreover, the obtained CLWSP-1 had strong antibacterial activity when exposed to Escherichia coli and Staphylococcus aureus, suggesting that CLWSP-1 may potentially contribute to the development of a natural preservative in the food industry.

Characterization and Suppressive Effect of Astragalus Polysaccharide in Glomerulonephritis Rats

2012 ◽  
Vol 554-556 ◽  
pp. 1713-1716 ◽  
Author(s):  
Shi Gang Li ◽  
Yong Qi Zhang
Keyword(s):  
Structural Characteristics ◽  
Average Molecular Weight ◽  
Periodate Oxidation ◽  
Gel Permeation Chromatography ◽  
Suppressive Effect ◽  
Protein Quantification ◽  
Water Soluble ◽  
Hot Water ◽  
Morphological Observation ◽  
Soluble Polysaccharide

A water-soluble polysaccharide named as APS was isolated from the roots of Astragalus membranaceus by hot water extraction, anion-exchange and gel-permeation chromatography and tested for its renal protective effect. Its structural characteristics were investigated by FTIR, AMLC, NMR spectroscopy, GLC–MS, methylation analysis, periodate oxidation and Smith degradation. Based on the data obtained, APS was found to be a α - (1-4)-D -glucan, with a single α- D-glucose at the C-6 position every nine residue, on average, along the main chain. The glucan has a weight-average molecular weight of about 3.6×104 Da. 24h Urine protein quantification and morphological observation, NF-κB protein expression and IL-2, IL-6 and TNF- α concentrations were significantly decreased by APS treatment, clearly suggesting that APS is effective in protecting against glomerulonephritis induced by C-BSA through the inhibition of NF- κB mediated-cytokine pathway.

Antitumor and immunomodulatory activity of water-soluble polysaccharide from Inonotus obliquus

2012 ◽  
Vol 90 (2) ◽  
pp. 870-874 ◽  
Author(s):  
Liuping Fan ◽  
Shaodong Ding ◽  
Lianzhong Ai ◽  
Kequan Deng
Keyword(s):  
Water Soluble ◽  
Immunomodulatory Activity ◽  
Inonotus Obliquus ◽  
Soluble Polysaccharide
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