scholarly journals SEQUENTIAL QUANTITATIVE ANALYSIS OF OVAL CELL PROLIFERATION IN THIOACETAMIDE TREATED RATS

2011 ◽  
Vol 20 (2) ◽  
pp. 105
Author(s):  
Abdul M Zaitoun ◽  
Christopher O Record ◽  
Hanan Al-Mardini
Keyword(s):  
Cell Proliferation ◽  
Bile Duct ◽  
Liver Injury ◽  
Oval Cells ◽  
Image Analysis System ◽  
Oval Cell ◽  
Hepatocyte Regeneration ◽  
Duct Cells ◽  
Complete Restoration ◽  
Analysis System

Oval cells are the progeny of facultative stem cells found in the periportal areas in response to liver injury in experimental animals and humans. The aims of this study were to describe the morphological, stereological and morphometric features of oval cells, and to compare them with those of bile duct cells and hepatocytes. It was also aimed to study the fate of oval cells by morphological and morphometric criteria. Rats were given thioacetamide to induce liver injury. The livers from experimental and control groups were processed routinely and stereological and morphometric analysis assessed using a computer image analysis system. Eight morphometric parameters were assessed in oval cells, bile duct cells and hepatocytes from control and experimental rats. Mitoses were observed in both oval cells and hepatocytes. Stereological area fraction analysis indicated that necrosis reached its maximum extent at 30 hours followed by regeneration and almost complete restoration of liver cell parenchyma at 132 hours. Oval cell proliferation reached a peak at 48-52 hours but was not apparent at 132 hours. Morphometric findings have shown increases in the nuclear diameter and nuclear area of oval cells with changes in the roundness and contours ratios of the nuclear membrane. It is concluded from this study that in thioacetamide treated rats, the liver responds to injury by bile ductal proliferation in the periportal areas which, accompanied by hepatocyte regeneration, leads to restoration of the hepatic parenchyma. At a subcellular morphological level the nuclei of oval cells showed a progressive change to a hepatocyte phenotype from that of a normal biliary cell, suggesting the differentiation of these cells into hepatocytes.

scholarly journals Dexamethasone inhibits the proliferation of hepatocytes and oval cells but not bile duct cells in rat liver

Hepatology ◽  
1998 ◽  
Vol 28 (2) ◽  
pp. 423-429 ◽  
Author(s):  
Peter Nagy ◽  
Andras Kiss ◽  
Janos Schnur ◽  
Snorri S. Thorgeirsson
Keyword(s):  
Bile Duct ◽  
Rat Liver ◽  
Oval Cells ◽  
Duct Cells ◽  
Proliferation Of Hepatocytes

scholarly journals Evaluation of astrocytoma cell proliferation using diffusion-weighted imaging: correlation with expression of proliferating cell nuclear antigen

2015 ◽  
Vol 6 (1) ◽  
pp. 265-270
Author(s):  
Kai Zhang ◽  
Chuanfu Li ◽  
Ying Liu ◽  
Li Li ◽  
Xiangshui Meng ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Low Grade ◽  
Image Analysis System ◽  
High Grade ◽  
Magnetic Resonance Scanner ◽  
Apparent Diffusion Coefficients ◽  
Analysis System ◽  
Proliferating Cell

AbstractThe purpose of this study was to analyze if there is a significant correlation between the results of diffusionweighted imaging (DWI) and the expression of proliferating cell nuclear antigen (PCNA) in astrocytomas. The DWI scans of 19 different-grade astrocytomas were obtained on a 3 T magnetic resonance scanner. The average regional apparent diffusion coefficients (ADC) were measured. The positive expression of PCNA was determined immunohistochemically by using streptavidin-peroxidase complex staining, and was quantified by calculating its calibrated opacity density (COD) using an image analysis system. The average regional ADC and PCNA COD of low grade and high grade astrocytomas were compared. Correlations between regional ADC and PCNA COD were analyzed. The average regional ADC of high grade astrocytomas was significantly (t = 10.169, P = 0.000) less (0.687 ± 0.225 x 10-3 mm2/s) than that of low grade astrocytomas (1.572 ± 0.333 x 10-3 mm2/s). The PCNA COD (0.343 ± 0.052) of high grade astrocytomas was significantly (t=-7.858, P=0.000) greater than that (0.194 ± 0.012) of low grade astrocytomas. There were strong negative correlations between regional ADC and PCNA COD (r = -0.801, P = 0.000). The results demonstrated that DWI is helpful in evaluating cell proliferation and preoperatively grading astrocytomas by measuring regional ADC.

scholarly journals Human liver organoid derived intra-hepatic bile duct cells support SARS-CoV-2 infection and replication and its comparison with SARS-CoV

2021 ◽  
Author(s):  
Vincent Chi-Hang Lui ◽  
Kenrie Pui-Yan Hui ◽  
Rosanna Ottakandathil Babu ◽  
Haibing Yue ◽  
Patrick Ho-Yu Chung ◽  
...  
Keyword(s):  
Bile Duct ◽  
Liver Injury ◽  
Human Liver ◽  
Liver Enzymes ◽  
Hepatic Bile ◽  
Hepatic Bile Duct ◽  
Duct Cells ◽  
Parenchymal Liver ◽  
Severe Liver Disease ◽  
Efficient Viral Replication

AbstractBackgroundAlthough the main route of infection for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the respiratory tract, liver injury is also commonly seen in many patients, as evidenced by deranged parenchymal liver enzymes. Furthermore, patients with severe liver disease have been shown to have higher mortality. Overall, the mechanism behind the liver injury remains unclear.Approach and resultsWe showed that intra-hepatic bile duct cells could be grown using a human liver organoid platform. The cholangiocytes were not only susceptible to SARS-CoV-2 infection, they also supported efficient viral replication. We also showed that SARS-CoV-2 replication was much higher than SARS-CoV.ConclusionOur findings suggested direct cytopathic viral damage being a mechanism for SARS-CoV-2 liver injury.

scholarly journals Impaired Preneoplastic Changes and Liver Tumor Formation in Tumor Necrosis Factor Receptor Type 1 Knockout Mice

2000 ◽  
Vol 192 (12) ◽  
pp. 1809-1818 ◽  
Author(s):  
Belinda Knight ◽  
George C.T. Yeoh ◽  
Kirsten L. Husk ◽  
Tina Ly ◽  
Lawrence J. Abraham ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Knockout Mice ◽  
Tumor Formation ◽  
Receptor Type ◽  
Oval Cells ◽  
Tnf Receptor ◽  
Oval Cell ◽  
Liver Carcinogenesis ◽  
Necrosis Factor

Hepatic stem cells (oval cells) proliferate within the liver after exposure to a variety of hepatic carcinogens and can generate both hepatocytes and bile duct cells. Oval cell proliferation is commonly seen in the preneoplastic stages of liver carcinogenesis, often accompanied by an inflammatory response. Tumor necrosis factor (TNF), an inflammatory cytokine, is also important in liver regeneration and hepatocellular growth. The experiments reported here explore the relationship among the TNF inflammatory pathway, liver stem cell activation, and tumorigenesis. We demonstrate that TNF is upregulated during oval cell proliferation induced by a choline-deficient, ethionine-supplemented diet and that it is expressed by oval cells. In TNF receptor type 1 knockout mice, oval cell proliferation is substantially impaired and tumorigenesis is reduced. Oval cell proliferation is impaired to a lesser extent in interleukin 6 knockout mice and is unchanged in TNF receptor type 2 knockout mice. These findings demonstrate that TNF signaling participates in the proliferation of oval cells during the preneoplastic phase of liver carcinogenesis and that loss of signaling through the TNF receptor type 1 reduces the incidence of tumor formation. The TNF inflammatory pathway may be a target for therapeutic intervention during the early stages of liver carcinogenesis.

scholarly journals Cell proliferation and renewal of normal hepatocytes and bile duct cells in adult mouse liver

2002 ◽  
Vol 22 (5) ◽  
pp. 419-425 ◽  
Author(s):  
Yasushi Magami ◽  
Takeshi Azuma ◽  
Hideto Inokuchi ◽  
Shinichiro Kokuno ◽  
Fuminori Moriyasu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bile Duct ◽  
Mouse Liver ◽  
Adult Mouse ◽  
Duct Cells ◽  
Adult Mouse Liver

scholarly journals Characterization of liver injury, oval cell proliferation and cholangiocarcinogenesis in glutathione S-transferase A3 knockout mice

2017 ◽  
Vol 38 (7) ◽  
pp. 717-727 ◽  
Author(s):  
Dana R. Crawford ◽  
Zoran Ilic ◽  
Ian Guest ◽  
Ginger L. Milne ◽  
John D. Hayes ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Liver Injury ◽  
Knockout Mice ◽  
Oval Cell ◽  
Glutathione S Transferase

scholarly journals Cell proliferation and oncogene expression after bile duct ligation in the rat: Evidence of a specific growth effect on bile duct cells

Hepatology ◽  
1995 ◽  
Vol 21 (4) ◽  
pp. 1070-1078
Author(s):  
Lorenzo Polimeno ◽  
Alessandro Azzarone ◽  
Qui Hua Zeng ◽  
Carmine Panella ◽  
Vladimir Subbotin ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bile Duct ◽  
Specific Growth ◽  
Bile Duct Ligation ◽  
Growth Effect ◽  
Oncogene Expression ◽  
Duct Cells ◽  
Duct Ligation

scholarly journals Development and Validation of an Image Analysis System for the Measurement of Cell Proliferation in Mammary Glands of Rats

2019 ◽  
Vol 47 (5) ◽  
pp. 634-644 ◽  
Author(s):  
Klaus Lindauer ◽  
Thomas Bartels ◽  
Petra Scherer ◽  
Mostafa Kabiri
Keyword(s):  
Cell Proliferation ◽  
Image Analysis ◽  
Mammary Glands ◽  
Absolute Difference ◽  
Image Analysis System ◽  
Proliferating Cells ◽  
Ki 67 ◽  
Validation Data ◽  
Manual Counting ◽  
Analysis System

Reliable detection and measurement of cell proliferation are essential in the preclinical assessment of carcinogenic risk of therapeutics. In this context, the assessment of mitogenic potential on mammary glands is crucial in the preclinical safety evaluation of novel insulins. The existing manual counting is time-consuming and subject to operator bias. To standardize the processes, make it faster, and resistant to errors, we developed a semiautomated image analysis system (CEPA software, which is open-source) for counting of proliferating cells in photomicrographs of mammary gland sections of rats labeled with Ki-67. We validated the software and met the predefined targets for specificity, accuracy, and reproducibility. In comparison to manual counting, the respective mean differences in absolute labeling indices (LIs) for CEPA software were 3.12% for user 1 and 3.05% for user 2. The respective regression analysis revealed a good correlation between the CEPA software user and manual counting. Moreover, the CEPA software showed enhanced reproducibility between independent users. The interuser variability is centered around 0 and the absolute difference was about 0.53% LI. Based on validation data, our software has superiority to the manual counting and is a valid and reliable tool for the routine analysis of cell proliferation in mammary glands from rats exposed to insulin analogs.

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