scholarly journals Development of a bovine sperm selection procedure for improvement of livestock fertility

2021 ◽  
Vol 12 (2) ◽  
pp. 75-84
Author(s):  
Maria-Portia B. NAGATA ◽  
Kenichi YAMASHITA
Synthesiology ◽  
2019 ◽  
Vol 12 (2) ◽  
pp. 75-83
Author(s):  
MariaPortia B. NAGATA ◽  
Kenichi YAMASHITA

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
MariaPortia B. Nagata ◽  
Junki Egashira ◽  
Naoto Katafuchi ◽  
Kenji Endo ◽  
Kazuko Ogata ◽  
...  

Abstract Background The application of cryopreservation and artificial insemination technology have contributed to the advancement of animal reproduction. However, a substantial proportion of spermatozoa undergoes alterations and loses their fertility during cryopreservation, rendering the frozen-thawed semen impractical for routine use. Cryopreservation is known to reduce sperm lifespan and fertility. Variation in cryosurvival of spermatozoa from different sires and even with the individual sire is common in artificial insemination (AI) centers. Our goal is to improve post-thawed semen quality by optimization of cryopreservation technique through sperm selection prior to cryopreservation process. Results Our strategy of sperm selection based on rheotaxis and thermotaxis (SSRT) on macrosale in a rotating fluid flow demonstrated the ability to maintain the original pre-freezing structural integrity, viability and biological function related to fertilization competence. This strategy has a positive effect on the cryosurvival and fertilizing abilities of spermatozoa as supported by the improvement on pregnancy rate of Japanese Black heifers and Holstein repeat breeders. This technique protected further sublethal damage to bovine spermatozoa (higher % cryosurvival than the control) and resulted in the improvement of DNA integrity. Prefreeze selected spermatozoa demonstrated slower and controlled capacitation than unprocessed control which is thought to be related to sperm longevity and consequently to appropriate timing during in vivo fertilization. Conclusions These results provide solid evidence that improvement of post-thawed semen quality by SSRT method is beneficial in terms of cryosurvival, longevity of post-thawed sperm, and optimization of in vivo fertilization, embryo development and calving as supported by the favorable results of field fertility study.


2008 ◽  
Vol 25 (5) ◽  
pp. 197-203 ◽  
Author(s):  
M. H. Nasr-Esfahani ◽  
S. Razavi ◽  
A. A. Vahdati ◽  
F. Fathi ◽  
M. Tavalaee

2016 ◽  
Vol 78 (5-9) ◽  
Author(s):  
Tatag Lindu Bhakti ◽  
Adhi Susanto ◽  
Paulus Insap Santosa ◽  
Diah Tri Widayati

This research proposed novel bovine sperm selection method based on sperm motility parameter to support bovine breeding industry. Sperm selection method consists of three main processes. First, decrease and holdbovine semen temperature at 4oC to reduce average sperm motility.Second, determine targeted sperm location which has highest motility within objective’s field of view after general motility observed decreasedinto 5% from initial value.Third, track targeted sperm and maintain holding temperature continuously until targeted sperm immotile and ready to be aspirated. Testing result showstemperature controller prototype can decrease bovine semen temperature safely without generate any intracellular ice. Micro actuator prototype can provide high motion performance exceed bovine sperm average velocity so it fully supporting motion detection software to perform real time bovine sperm tracking. Autofocus mechanism was succeedingincrease motion detection sensitivity using 4X, 10X and 40X objectives lens. All prototype devices developed in this research provide safely selection process to achieve high quality bovine sperm.


2012 ◽  
Vol 24 ◽  
pp. S8 ◽  
Author(s):  
Susana Cortes ◽  
Rocio Nunez-Calonge ◽  
Marta Gago ◽  
Leonor Ortega ◽  
Enrique Olaya ◽  
...  

1996 ◽  
Vol 12 (1) ◽  
pp. 33-42 ◽  
Author(s):  
Marco Perugini ◽  
Luigi Leone

The aim of this contribution is to present a new short adjective-based measure of the Five Factor Model (FFM) of personality, the Short Adjectives Checklist of BIg Five (SACBIF). We present the various steps of the construction and the validation of this instrument. First, 50 adjectives were selected with a selection procedure, the “Lining Up Technique” (LUT), specifically used to identify the best factorial markers of the FFM. Then, the factorial structure and the psychometric properties of the SACBIF were investigated. Finally, the SACBIF factorial structure was correlated with some main measures of the FFM to establish its construct validity and with some other personality dimensions to investigate how well these dimensions could be represented in the SACBIF factorial space.


2018 ◽  
Vol 17 (3) ◽  
pp. 155-160 ◽  
Author(s):  
Daniel Dürr ◽  
Ute-Christine Klehe

Abstract. Faking has been a concern in selection research for many years. Many studies have examined faking in questionnaires while far less is known about faking in selection exercises with higher fidelity. This study applies the theory of planned behavior (TPB; Ajzen, 1991 ) to low- (interviews) and high-fidelity (role play, group discussion) exercises, testing whether the TPB predicts reported faking behavior. Data from a mock selection procedure suggests that candidates do report to fake in low- and high-fidelity exercises. Additionally, the TPB showed good predictive validity for faking in a low-fidelity exercise, yet not for faking in high-fidelity exercises.


2013 ◽  
Vol 3 (1) ◽  
pp. 19-27 ◽  
Author(s):  
Yvonne Pecena ◽  
Doris Keye ◽  
Kristin Conzelmann ◽  
Dietrich Grasshoff ◽  
Peter Maschke ◽  
...  

The job of an air traffic controller (ATCO) is very specific and demanding. The assessment of potential suitable candidates requires a customized and efficient selection procedure. The German Aerospace Center DLR conducts a highly selective, multiple-stage selection procedure for ab initio ATCO applicants for the German Air Navigation Service Provider DFS. Successful applicants start their training with a training phase at the DFS Academy and then continue with a unit training phase in live traffic. ATCO validity studies are scarcely reported in the international scientific literature and have mainly been conducted in a military context with only small and male samples. This validation study encompasses the data from 430 DFS ATCO trainees, starting with candidate selection and extending to the completion of their training. Validity analyses involved the prediction of training success and several training performance criteria derived from initial training. The final training success rate of about 79% was highly satisfactory and higher than that of other countries. The findings demonstrated that all stages of the selection procedure showed predictive validity toward training performance. Among the best predictors were scores measuring attention and multitasking ability, and ratings on general motivation from the interview.


Methodology ◽  
2018 ◽  
Vol 14 (4) ◽  
pp. 177-188 ◽  
Author(s):  
Martin Schultze ◽  
Michael Eid

Abstract. In the construction of scales intended for the use in cross-cultural studies, the selection of items needs to be guided not only by traditional criteria of item quality, but has to take information about the measurement invariance of the scale into account. We present an approach to automated item selection which depicts the process as a combinatorial optimization problem and aims at finding a scale which fulfils predefined target criteria – such as measurement invariance across cultures. The search for an optimal solution is performed using an adaptation of the [Formula: see text] Ant System algorithm. The approach is illustrated using an application to item selection for a personality scale assuming measurement invariance across multiple countries.


2020 ◽  
pp. 47-50
Author(s):  
N. V. Saraeva ◽  
N. V. Spiridonova ◽  
M. T. Tugushev ◽  
O. V. Shurygina ◽  
A. I. Sinitsyna

In order to increase the pregnancy rate in the assisted reproductive technology, the selection of one embryo with the highest implantation potential it is very important. Time-lapse microscopy (TLM) is a tool for selecting quality embryos for transfer. This study aimed to assess the benefits of single-embryo transfer of autologous oocytes performed on day 5 of embryo incubation in a TLM-equipped system in IVF and ICSI programs. Single-embryo transfer following incubation in a TLM-equipped incubator was performed in 282 patients, who formed the main group; the control group consisted of 461 patients undergoing single-embryo transfer following a traditional culture and embryo selection procedure. We assessed the quality of transferred embryos, the rates of clinical pregnancy and delivery. The groups did not differ in the ratio of IVF and ICSI cycles, average age, and infertility factor. The proportion of excellent quality embryos for transfer was 77.0% in the main group and 65.1% in the control group (p = 0.001). In the subgroup with receiving eight and less oocytes we noted the tendency of receiving more quality embryos in the main group (р = 0.052). In the subgroup of nine and more oocytes the quality of the transferred embryos did not differ between two groups. The clinical pregnancy rate was 60.2% in the main group and 52.9% in the control group (p = 0.057). The delivery rate was 45.0% in the main group and 39.9% in the control group (p > 0.050).


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