scholarly journals Typing of Candidemia Agents of Candida albicans Isolates by Molecular Methods and Investigation of SAP4 Gene Presence

2020 ◽  
Vol 25 (3) ◽  
pp. 316-324
Author(s):  
Kemal Bilgin ◽  
Asuman Birinci ◽  
Tuba Yıldırım
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Albicans ◽  
Virulence Factors ◽  
Infectious Agent ◽  
Molecular Methods ◽  
Chain Reaction ◽  
Mortality And Morbidity ◽  
Typing Methods ◽  
Polymerase Chain ◽  
Secreted Aspartyl Proteinase

Introduction: In recent years, increasing infections due to fungi have drawn attention. Especially, Candida albicans is the most frequent infectious agent with its virulence factors. Furthermore, because C. albicans is a nosocomial infection agent, it can lead an increase in mortality and morbidity. In this study, it was aimed to genotype C. albicans strains that caused candidemia by molecular methods and to investigate one of the most important virulence factors, Secreted Aspartyl Proteinase 4 (SAP4). Materials and Methods: Our study included 50 C. albicans strains isolated from blood cultures. The isolates were identified both phenotypically and genotypically. Arbitrarily-Primed Polymerase Chain Reaction (AP-PCR) and Pulsed Field Gel ElectrophoresisElectrophoretic Karyotype Analysis (PFGE-EK) methods were used for molecular genotyping of the strains. Furthermore, the presence of SAP4 a virulence factor for C. albicans was investigated by polymerase chain reaction (PCR). Results: The 50 C. albicans isolates included in our study were separated to 26 genotypes by AP-PCR, 41 genotypes by PFGE-EK, and SAP4 was detected in 49 of the 50 isolates. Conclusion: In conclusion, dominant genotypes among the C. albicans isolates were not detected. Results of both molecular typing methods revealed that C. albicans isolates were generally endogenous. Furthermore, SAP4 gene was detected in 98% of the isolates. A considerable presence of this gene in blood sample isolates suggests that it contributes to systemic infections. More elaborative studies on the function of SAP4 gene in endogenous infections of C. albicans may contribute to the prevention of these infections in the future.

Detection of Candida albicans by Polymerase Chain Reaction from Formalin-Fixed Paraffin-Embedded Tissue Specimens.

2000 ◽  
Vol 42 (2) ◽  
pp. 166-168
Author(s):  
Swe Swe Win ◽  
Kei Sakamoto ◽  
Teruo Amagasa ◽  
Ba Myint ◽  
Minoru Takagi
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Albicans ◽  
Chain Reaction ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Polymerase Chain ◽  
Tissue Specimens ◽  
Formalin Fixed

scholarly journals Molecular characterization of Candida dubliniensis and Candida albicans in the oral cavity of drug abusers using duplex polymerase chain reaction

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Parastoo Hassani Abharian ◽  
Parvin Dehghan ◽  
Peyman Hassani Abharian ◽  
Sepideh Tolouei
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Albicans ◽  
Oral Cavity ◽  
Candida Species ◽  
Species Complex ◽  
Candida Dubliniensis ◽  
Drug Abusers ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Duplex Polymerase Chain Reaction

  Background and Purpose: Candida dubliniensis is closely related to the most pathogenic and prevalent yeast, namely C. albicans. Candida species can opportunistically overgrow in vulnerable individuals and cause a variety of diseases. The current study aimed to identify and isolate C. dubliniensis species present in the Candida albicans species complex identified in the oral cavity of drug abusers. Materials and Methods: This study was conducted on 53 strains of C. albicans species complex, isolated from the oral mucosa of drug abusers in Isfahan, Iran. DNA extraction was accomplished through boiling procedure. Duplex polymerase chain reaction (PCR) was performed to amplify ITS1-5.8S-ITS2 region using four specific primers. Fungal species were identified based on the difference in the size of the bands created in the Agarose gel. Results: Out of the 53 isolates under study, 30 (56.6%) and 14 (26.4%) samples were identified as C. albicans and C. dubliniensis, respectively. In the remaining 9 samples (17%), both types of Candida species were confirmed. Conclusion: The findings of the present study revealed the presence of a noticeable amount of C. dubliniensis in the oral cavity of drug abusers. Therefore, the probable presence of this fungus should be considered during the examination of oral infection among this group. To date, no research has directly investigated this issue in Iran.

Rapid discrimination between Candida albicans and Candida dubliniensis by using real-time polymerase chain reaction

2007 ◽  
Vol 58 (3) ◽  
pp. 367-369 ◽  
Author(s):  
Ferenc Somogyvari ◽  
Ilona Doczi ◽  
Julianna Serly ◽  
Suhail Ahmad ◽  
Elizabeth Nagy
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Albicans ◽  
Real Time ◽  
Candida Dubliniensis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Rapid Discrimination

Detection of virulence factors of Escherichia coli from children by multiplex polymerase chain reaction

2005 ◽  
Vol 60 (2) ◽  
pp. 170-173 ◽  
Author(s):  
M. Arisoy ◽  
D. Aysev ◽  
M. Ekim ◽  
D. Özel ◽  
S. K. Köse ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Virulence Factors ◽  
Multiplex Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of Candida albicans DNA in Serum by Polymerase Chain Reaction

1994 ◽  
Vol 26 (4) ◽  
pp. 479-485 ◽  
Author(s):  
Erja Chryssanthou ◽  
Birger Andersson ◽  
Björn Petrini ◽  
Sven Löfdahl ◽  
Jan Tollemar
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Albicans ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of Enterococcus faecalis and Candida albicans in previously root-filled teeth in a population of Gujarat with polymerase chain reaction

2013 ◽  
Vol 4 (1) ◽  
pp. 62 ◽  
Author(s):  
Bruhvi Poptani ◽  
Murali Sharaff ◽  
G Archana ◽  
Vaishali Parekh
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Chain Reaction ◽  
Polymerase Chain

Bacterial Etiology for Chronic Villitis is Not Supported by Polymerase Chain Reaction for 16S rRNA DNA

2005 ◽  
Vol 8 (6) ◽  
pp. 647-653 ◽  
Author(s):  
Linda M. Ernst ◽  
Jill Crouch ◽  
Henry Rinder ◽  
John Greg Howe
Keyword(s):  
Polymerase Chain Reaction ◽  
16S Rrna ◽  
Plasma Cells ◽  
Infectious Agent ◽  
Pcr Primers ◽  
Proteinase K ◽  
Chain Reaction ◽  
Direct Spread ◽  
Polymerase Chain ◽  
Chronic Villitis

Chronic villitis is characterized by chorionic villi infiltrated by lymphocytes, histiocytes, and sometimes plasma cells. In a small percentage of cases, an infectious agent can be demonstrated within areas of chronic villitis. However, the pathogenesis of most lesions is idiopathic. Chronic villitis may represent the direct spread of chronic endometrial infection by bacterial organisms that are particularly problematic for culture. To test this hypothesis, polymerase chain reaction (PCR) using primers for the universal bacterial 16S rRNA DNA was performed on DNA extracted from areas of chronic villitis selected from placentas in the Yale Pathology database. Specific areas of chronic villitis were first confirmed by examination of sections stained with hematoxylin and eosin and then removed from archived paraffin blocks. Control tissue spiked with known bacterial counts was also prepared to test the sensitivity of the experiment. All tissue was deparaffinized, dehydrated, and digested with proteinase K. DNA extraction was performed with the Gentra Puregene kit. PCR was done using primers p11 and p13 for the 16S rRNA DNA. The 233-bp amplified target product was identified by agarose gel electrophoresis. Nineteen specimens with multifocal chronic villitis without confinement to anchoring villi were studied. None of the chronic villitis specimens had a demonstrable product using the PCR primers for 16S rRNA DNA, despite adequate DNA in the samples and controls. The assay was sensitive down to approximately 1500 bacteria per specimen. In conclusion, these data do not support a bacterial etiology for chronic villitis.

Use of blood cultures in critically ill patients

2000 ◽  
Vol 20 (1) ◽  
pp. 45-50 ◽  
Author(s):  
R Henker
Keyword(s):  
Polymerase Chain Reaction ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
New Technology ◽  
Infectious Agent ◽  
Blood Cultures ◽  
Chain Reaction ◽  
Blood Samples ◽  
Polymerase Chain ◽  
Timely Treatment

Infection, bacteremia, and sepsis are frequent complications in critically ill patients. Ideally, the infectious agent is readily identified to facilitate timely treatment to promote the patient's recovery. Use of blood cultures is one method of identifying the pathogen. Fever is the primary indicator for obtaining blood samples for culture, but other indicators may be considered, depending on the patient's medical history and condition. Use of appropriate techniques when collecting blood samples for culture will decrease contamination and improve the likelihood of identification of the infectious agent. One new technique being tested for the identification of pathogens that cause bacteremia involves genetic technology and the polymerase chain reaction. The polymerase chain reaction is used to identify the DNA of bacteria that are present in the blood. Blood cultures may not always result in identification of the pathogen because the organism may not grow once placed in culture medium. This new method that uses the polymerase chain reaction may be more sensitive than blood cultures because it requires only DNA from bacteria. Although early studies have not been conclusive in terms of the benefits of this new technology, additional research will improve methods for identification of pathogens in critically ill patients.

New method for detection of Candida albicans in human blood by polymerase chain reaction.

1993 ◽  
Vol 31 (12) ◽  
pp. 3344-3347 ◽  
Author(s):  
Y Miyakawa ◽  
T Mabuchi ◽  
Y Fukazawa
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Albicans ◽  
Human Blood ◽  
New Method ◽  
Chain Reaction ◽  
Polymerase Chain
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