The effect of Medicago arabica, M. hybrida and M. sativa saponins on the growth and development of Fusarium oxysporum Schlecht f. sp. tulipae apt.

Anna Jarecka; Alicja Saniewska; Zbigniew Biały; Marian Jurzysta

doi:10.5586/aa.2008.043

The effect of Medicago arabica, M. hybrida and M. sativa saponins on the growth and development of Fusarium oxysporum Schlecht f. sp. tulipae apt.

Acta Agrobotanica ◽

10.5586/aa.2008.043 ◽

2012 ◽

Vol 61 (2) ◽

pp. 147-155 ◽

Cited By ~ 1

Author(s):

Anna Jarecka ◽

Alicja Saniewska ◽

Zbigniew Biały ◽

Marian Jurzysta

Keyword(s):

Fusarium Oxysporum ◽

Growth And Development ◽

Inhibitory Effect ◽

Mycelium Growth ◽

Medicagenic Acid ◽

Colony Forming Units ◽

Tulip Bulbs

In the present work it was shown that total saponins originated from M. hybrida and M. sativa substantially limited mycelium growth of F. oxysporum f. sp. tulipae and symptoms of fusariosis on tulip bulbs. Out of 15 individual tested saponins originated from M. arabica, M. hybrida and M. sativa, four compounds: 3-O-[β-D-glucopyranosyl (1→2)α-L-arabinopyranosyl] hederagenin, hederagenin 3-O-β-D-glucopyranoside, medicagenic acid, medicagenic acid 3-O-β-D-glucopyranoside had the strongest inhibitory effect on mycelium growth of Fusarium oxysporum f. sp. tulipae on PDA medium. The total saponins from M. arabica, M. hybrida and M. sativa inhibited the number of colony forming units of Fusarium oxysporum f. sp. tulipae in artificially infested substrate. The use of saponins originated from Medicago as a fungicide is suggested.

The role of gum induced by Fusarium oxysporum Schlecht. Snyd. et Hans. f.sp. tulipae Apt. in tulip bulbs on growth and development of the pathogen

Plant Protection Science ◽

10.17221/10515-pps ◽

2017 ◽

Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽

pp. 432-435

Author(s):

A. Saniewska

Keyword(s):

Fusarium Oxysporum ◽

Growth And Development ◽

Mycelium Growth ◽

Tulip Bulbs ◽

Bacto Agar

It was showed that gums induced by Fusarium oxysporum f.sp. tulipae in tulip bulbs applied to mineral Czapek-Dox Broth with Bacto Agar (CzDA) medium, containing sucrose substantially stimulated abundant growth of mycelium and sporulation of the pathogen. Addition of arabinose, xylose and their mixture, sugars occurring in tulip gum polysaccharide, to CzDA medium caused that mycelium was very sparse and sporulation was only slightly stimulated by arabinose. Mycelium growth on mineral CzDA medium without sucrose, was poor and sparse but addition of gum to the medium caused formation of abundant mycelium and increased sporulation of the pathogen. It is possible that polysaccharide of tulip gum may act mainly as elicitor and partially as substrate in regulation of mycelium growth and sporulation of Fusarium oxysporum f.sp. tulipae.

The effect of D,L-β-aminobutyric acid on the growth and development of Fusarium oxysporum f. sp. tulipae (Apt.)

Acta Agrobotanica ◽

10.5586/aa.2007.012 ◽

2012 ◽

Vol 60 (1) ◽

pp. 101-105 ◽

Cited By ~ 1

Author(s):

Anna Jarecka ◽

Alicja Saniewska

Keyword(s):

Fusarium Oxysporum ◽

Root System ◽

Growth And Development ◽

Linear Growth ◽

Aminobutyric Acid ◽

Fresh Weight ◽

Mycelium Growth ◽

Disease Symptoms ◽

Tulip Bulbs ◽

Inducing Resistance

The effect of D,L-β-aminobutyric acid (BABA) on the growth and development of the root system and the development of fusariosis on tulip bulbs cv. Apeldoorn infected by Fusarium oxysporum f. sp. tulipae (F.ox.t. 218) was studied. The length and fresh weight of roots, the development of fusariosis on bulbs and the linear growth of mycelium of F.ox.t. 218 on PDA medium were measured. Preventively used BABA at a concentration of 100, 250 and 300 µg·cm-3 for soaking uncooled and cooled tulip bulbs greatly inhibited the development of fusariosis on the root system; the length and fresh weight of roots were similar to those of the bulbs not inoculated with F.ox.t. 218. At a concentration of 100 µg·cm-3;, BABA used for soaking bulbs limited the development of fusariosis on scales in about 50% and the concentration of 200 µg·cm-3 totally inhibited the disease symptoms induced by F.ox.t. 218. At a concentration of 100 - 1000 µg·cm-3, BABA did not inhibit the mycelium growth of F.ox.t. 17 and F.xo.t. 218 on PDA medium. This study suggests that BABA protects tulip roots and bulb scales against F. oxysporum f. sp. tulipae by inducing resistance in these organs and has no direct influence on the pathogen.

Antifungal activity of saponins originated from Medicago hybrida against some ornamental plant pathogens

Acta Agrobotanica ◽

10.5586/aa.2006.061 ◽

2012 ◽

Vol 59 (2) ◽

pp. 51-58 ◽

Cited By ~ 9

Author(s):

Alicja Saniewska ◽

Anna Jarecka ◽

Zbigniew Biały ◽

Marian Jurzysta

Keyword(s):

Antifungal Activity ◽

Fusarium Oxysporum ◽

Botrytis Cinerea ◽

Plant Pathogens ◽

Pathogenic Fungi ◽

Ornamental Plant ◽

Mycelium Growth ◽

Medicagenic Acid ◽

Fungitoxic Effect

Antifungal activity of total saponins originated from roots of Medicago hybrida (Pourret) Trautv. were evaluated in vitro against six pathogenic fungi and eight individual major saponin glycosides were tested against one of the most susceptible fungi. The total saponins showed fungitoxic effect at all investigated concentrations (0.01%, 0.05% and 0.1%) but their potency was different for individual fungi. The highest saponin concentration (0.1%) was the most effective and the inhibition of Fusarium oxysporum f. sp. callistephi, Botrytis cinerea, Botrytis tulipae, Phoma narcissi, Fusarium oxysporum f. sp. narcissi was 84.4%, 69.9%, 68.6%, 57.2%, 55.0%, respectively. While Fusarium oxysporum Schlecht., a pathogen of Muscari armeniacum, was inhibited by 9.5% only. Eight major saponin glycosides isolated from the total saponins of M. hybrida roots were tested against the mycelium growth of Botrytis tulipae. The mycelium growth of the pathogen was greatly inhibited by hederagenin 3-O-β-D-glucopyranoside and medicagenic acid 3-O-β-D-glucopyranoside. Medicagenic acid 3-O-β-D-glucuronopyranosyl-28-O-β-D-glucopyranoside and oleanolic acid 3-O-[β-D-glucuronopyranosyl(1→2)-α-L-galactopyranosyl]-28-O-β-D-glucopyranoside showed low fungitoxic activity. Medicagenic acid 3-O-a-D-glucopyranosyl- 28-O-β-D-glucopyranoside, hederagenin 3-O-[α-L- hamnopyranosyl(1→2)-β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl]- 28-O-α-D-glucopyranoside and hederagenin 3-O-β-D-glucuronopyranosyl-28-O-β-D- lucopyranoside did not limit or only slightly inhibited growth of the tested pathogen. While 2β, 3β-dihydroxyolean-12 ene-23-al-28-oic acid 3-O-β-D-glucuronopyranosyl-28-O-β-D-glucopyranoside slightly stimulated mycelium growth of B. tulipae.

The effect of chitosan on limitation of growth and development of some pathogenic fungi for ornamental plants

Acta Agrobotanica ◽

10.5586/aa.2001.002 ◽

2013 ◽

Vol 54 (1) ◽

pp. 17-29 ◽

Cited By ~ 2

Author(s):

Alicja Saniewska

Keyword(s):

Fusarium Oxysporum ◽

Inhibitory Action ◽

Marked Effect ◽

Ornamental Plants ◽

Pathogenic Fungi ◽

Inhibitory Effect ◽

Crab Shell ◽

Necrotic Spots ◽

Tulip Bulbs

The inhibitory effect of crab-shell chitosan, medium (200-800 cps) and high molecular weight ( 800-2000 cps) (purchased from Sigma-Aldrich Chemicals) toward Alternaria alternata, Botrytis tulipae, Fiisarium oxysporum f. sp. callistephi, Fusarium oxysporum f. sp. tulipae, Phoma narcissi and Phoma poolensis was evaluated in vitro and in vivo. The chitosan evidently inhibited in vitro growth of all tested pathogens, with a marked effect at higher concentrations above 200 μg/cm3. Chitosan at a concentration of 1,25; 2,5 and 5,0 mg/cm3 didn't have inhibitory action in appearance of fungi growth on naturally contaminated Callistephus chinensis seeds. At the same concentrations, chitosan applied as bulb scales dressing of Hymenocallis narcissiflora bulbs, before inoculation or after inoculation with Phoma narcissi, inhibited the development of necrotic spots on scales. Chitosan used preventively or curatively at a concentrations of 1,25; 2,5 and 5,0 mg/cm3 indicated inhibitory effect on development of Fusarium oxysporum f. sp. tulipae on tulip bulbs. Chitosan at a concentration of 10 mg/cm3 applied preventively (first spray 12th June) was very effective in the control of Puccinia antirrhini on snapdragon in the field. The strongest inhibitory effect was observed on snapdragon treated 8 times at week intervals.

The effect of salicylic acid and acetylsalicylic acid on red pigment formation in mechanically wounded scales of Hippeastrum x hybr. hort. and on the growth and development of Phoma narcissi

Acta Agrobotanica ◽

10.5586/aa.2005.035 ◽

2012 ◽

Vol 58 (2) ◽

pp. 81-90

Author(s):

Alicja Saniewska ◽

Marcin Horbowicz ◽

Marian Saniewski

Keyword(s):

Salicylic Acid ◽

Acetylsalicylic Acid ◽

Growth And Development ◽

Inhibitory Effect ◽

Basal Plate ◽

Red Pigment ◽

Mycelium Growth ◽

Pigment Formation

Various organs of Hippeastrum infected by Phoma narcissi, infested with mite, Steneotarsonemus laticeps or mechanically wounded, produce red pigment on the surface of injured tissues. The aim of the present work was to study the effect of salicylic (SA) and acetylsalicylic acids (ASA) (inhibitors of biosynthesis of jasmonates and ethylene) on red pigment formation in wounded scales of bulbs of Hippeastrum and on the mycelium growth of P. narcissi in vivo and in vitro. SA and ASA at a concentration of 1 and 2 mM partially inhibited the formation of red pigment in wounded scales, first of all in first 2 days after treatment. The growth and development of P. narcissi on basal plate and scales of longitudinally cut Hippeastrum bulb treated with SA and ASA (1 and 2 mM) was similar as in control. SA (50 µg·cm-3) and ASA (250 µg·cm-3) inhibited the mycelium growth of P. narcissi on PDA medium, and concentration of 1000 µg·cm-3 of both almost totally inhibited the mycelium growth of the pathogen. Inhibitory effect of SA and ASA on the formation of red pigment in wounded scales of Hippeastrum may be caused by lowered biosynthesis and accumulation of jasmonates.

Influence of Bacillus polymyxa on the growth and development of Fusarium oxysporum f. sp. tulipae

Acta Agrobotanica ◽

10.5586/aa.1999.001 ◽

2013 ◽

Vol 52 (1-2) ◽

pp. 5-10

Author(s):

Alicja Saniewska

Keyword(s):

Fusarium Oxysporum ◽

Spore Germination ◽

Growth And Development ◽

Antagonistic Effect ◽

Potato Dextrose Agar ◽

Bacillus Polymyxa ◽

Dual Cultures ◽

Tulip Bulbs

Antagonistic effect of Bacillus polymyxa, strain S13, toward Fusarium oxysporum f. sp. tulipae was evaluated iii vitro and in vivo. The growth of the pathogen was greatly inhibited in dual cultures with Bacillus polymyxa on potato dextrose agar. Suspension of B. polymyxa and its filtrate substantially inhibited spore germination and development of Fusarium oxysporuum f. sp. tulipae on tulip bulbs.

Antifungal activity of Morinda citrifolia leaf extracts against Fusarium oxysporum

Indonesian Journal of Biotechnology and Biodiversity ◽

10.47007/ijobb.v4i1.51 ◽

2020 ◽

Vol 4 (1) ◽

pp. 20-26

Author(s):

Oktira Roka Aji ◽

Yuni Rohmawati

Keyword(s):

Antifungal Activity ◽

Fusarium Oxysporum ◽

Ethanol Extract ◽

Inhibitory Effect ◽

Morinda Citrifolia ◽

Leaf Extracts ◽

Mycelium Growth ◽

Pathogen Control ◽

Negative Impacts

Fusarium oxysporum is an important disease that causes wilt disease in plants. Fungal pathogen control using synthetic fungicides can cause negative impacts on the environment. Morinda citrifolia is one of the herbs that is known for many benefits. M. citrifolia leaf contains anthraquinone which have potential as antifungal agents. In this study, in vitro anti-fungal assay was conducted against F. oxysporum to analyze the antifungal activity of ethanol extract of M. citrifolia leaves. In vitro evaluation was carried out using poisoned food technique at four different concentrations i.e., 20%, 40%, 60% and 80%. The results showed concentration 60% gave the highest inhibitory effect (21,82%) on F. oxysporum mycelium growth.

Potential antifungal effects of silver nanoparticles (AgNPs) of different sizes against phytopathogenic Fusarium oxysporum f. sp. radicis-lycopersici (FORL) strains

SN Applied Sciences ◽

10.1007/s42452-021-04524-5 ◽

2021 ◽

Vol 3 (4) ◽

Author(s):

Ilgin Akpinar ◽

Muammer Unal ◽

Taner Sar

Keyword(s):

Silver Nanoparticles ◽

Fusarium Oxysporum ◽

Antifungal Agents ◽

New Technology ◽

Economic Loss ◽

Phytopathogenic Fungi ◽

Growth Media ◽

Mycelium Growth ◽

Antifungal Effects ◽

Critical Problems

AbstractFusarium species are the primary fungal pathogen affecting agricultural foodstuffs both in crop yield and economic loss. Due to these problems, control of phytopathogenic fungi has become one of the critical problems around the World. Nanotechnology is a new technology with potential in many fields, including agriculture. This study focused on determining potential effects of silver nanoparticles (AgNPs) with different nanosizes (3, 5, 8 and 10 nm) and at different concentrations (12.5–100 ppm) against phytopathogenic Fusarium oxysporum f. sp. radicis-lycopersici (FORL) strains. The maximum antifungal activity was achieved by decreasing nanosize and increasing concentration of AgNPs. Mycelium growth abilities were decreased about 50%, 75% and 90% by AgNPs treatment with 3 nm sizes at 25 ppm, 37.5 ppm and 50 ppm concentrations, respectively. The productivity of fungal biomass in the liquid growth media was found to be too limited at the 25–37.5 ppm of AgNPs concentrations with all sizes. In addition, both septation number and dimensions of micro- and macroconidia were found to be gradually decreased with the application of silver nanoparticles. This work showed that the low concentration of AgNPs could be used as potential antifungal agents and applied for control of phytopathogens.

Brassinolide Enhances the Level of Brassinosteroids, Protein, Pigments, and Monosaccharides in Wolffia arrhiza Treated with Brassinazole

Plants ◽

10.3390/plants10071311 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1311

Author(s):

Magdalena Chmur ◽

Andrzej Bajguz

Keyword(s):

Plant Growth ◽

Growth And Development ◽

Inhibitory Effect ◽

Dependent Manner ◽

Plant Growth And Development ◽

Concentration Dependent Manner ◽

Spectrophotometric Methods ◽

Synthetic Inhibitor ◽

Wolffia Arrhiza ◽

First Time

Brassinolide (BL) represents brassinosteroids (BRs)—a group of phytohormones that are essential for plant growth and development. Brassinazole (Brz) is as a synthetic inhibitor of BRs’ biosynthesis. In the present study, the responses of Wolffia arrhiza to the treatment with BL, Brz, and the combination of BL with Brz were analyzed. The analysis of BRs and Brz was performed using LC-MS/MS. The photosynthetic pigments (chlorophylls, carotenes, and xanthophylls) levels were determined using HPLC, but protein and monosaccharides level using spectrophotometric methods. The obtained results indicated that BL and Brz influence W. arrhiza cultures in a concentration-dependent manner. The most stimulatory effects on the growth, level of BRs (BL, 24-epibrassinolide, 28-homobrassinolide, 28-norbrassinolide, catasterone, castasterone, 24-epicastasterone, typhasterol, and 6-deoxytyphasterol), and the content of pigments, protein, and monosaccharides, were observed in plants treated with 0.1 µM BL. Whereas the application of 1 µM and 10 µM Brz caused a significant decrease in duckweed weight and level of targeted compounds. Application of BL caused the mitigation of the Brz inhibitory effect and enhanced the BR level in duckweed treated with Brz. The level of BRs was reported for the first time in duckweed treated with BL and/or Brz.

Effects of sugars on the growth and chlorophyll content in excised tulip stem in the presence of Indole-3-acetic acid

Journal of Fruit and Ornamental Plant Research ◽

10.2478/v10290-012-0008-6 ◽

2012 ◽

Vol 20 (1) ◽

pp. 97-114 ◽

Cited By ~ 1

Author(s):

Elżbieta Węgrzynowicz-Lesiak ◽

Marian Saniewski ◽

Justyna Góraj ◽

Marcin Horbowicz ◽

Kensuke Miyamoto ◽

...

Keyword(s):

Acetic Acid ◽

Chlorophyll Content ◽

Inhibitory Effect ◽

Flower Bud ◽

Chlorophyll Contents ◽

Delayed Senescence ◽

Sugar Response ◽

Tulip Bulbs ◽

Indole 3 Acetic Acid ◽

Stem Segments

ABSTRACT The purpose of this study was to clarify the effect of sucrose on auxin-induced growth of stem excised from growing tulips and excised directly from cooled and not cooled bulbs, and on the growth of excised IV internode from growing plants in the presence of auxin. In all cases flower bud was replaced by IAA (indole-3- acetic acid, 0.1%, w/w in lanolin) and basal part of excised segments of stem was kept in distilled water or in solution of various sugars at different concentrations. IAA-induced growth of excised stems isolated from growing tulips was inhibited by sucrose at concentrations of 5.0% and 10.0%, but sucrose at 1.25% and 2.5% did not. Sucrose at all concentrations used evidently delayed senescence and increased chlorophyll contents in excised stems in the presence of IAA. Sucrose induced stiffing in isolated stems in the presence of IAA, and much less infective by pathogen in comparison to stem treated with IAA only. Mannitol and sorbitol at concentrations of 5.0% and 10.0% substantially inhibited IAA-induced growth of stem segments. Stem segments excised from cooled and not cooled tulip bulbs were more sensitive than those isolated from growing shoots due to application of sucrose and glucose; more inhibitory effect was observed. Sucrose at concentrations of 5.0% and 10.0% only slightly inhibited growth of IV internode treated with IAA and all concentrations of sucrose (1.25%, 2.5%, 5.0% and 10.0%) substantially increased chlorophyll content. The possible mode of actions of sucrose interacting with auxin to regulate stem growth is also discussed although sugar response is complicated by the fact that plants have multiple sugar-response pathways.