scholarly journals Organization of the cytoplasmic reticulum in the central vacuole of parenchyma cells in Allium cepa L.

2015 ◽  
Vol 44 (1) ◽  
pp. 115-122
Author(s):  
Tomasz J. Wodzicki ◽  
Claud L. Brown ◽  
Walter J. Humpherys

An elaborate and complex cytoplasmic reticulum composed of fine filaments and lamellae ranging from 0.1 to 4 microns in size is revealed by viewing the central vacuole of onion bulb parenchyma cells with the scanning election microscope. The larger cytoplasmic strands, visible with the light microscope, are composed of numerous smaller filaments (some tubular) which might explain the observed bidirectional movement of particles in these larger strands. The finely divided cytoplasmic network of filaments is continuous with the parietal cytoplasm inclosing the vacuolar sap. In these highly vacuolated cells the mass of the protoplast is in the form of an intravacuolar reticulum immersed in the cell sap. The probable significance of the vacuolar sap in relation to physiological processes of the cell is discussed.

2020 ◽  
Vol 145 ◽  
pp. 111847 ◽  
Author(s):  
Diogenes dos Santos Dias ◽  
Caio Gomide Otoni ◽  
Robson Rosa da Silva ◽  
Andreia Bagliotti Meneguin ◽  
Luiz Henrique Capparelli Mattoso ◽  
...  

2013 ◽  
Vol 39 (2) ◽  
pp. 325-339
Author(s):  
Roch W. Doruchowski

The object of the studies in 1977 was a population of 15 F<sub>1</sub> hybrids, 11 F<sub>2</sub> progenies, 16 parental forms (8 malesterile A lines and 3 inbred C lines). Heterosis of onion bulb weight, height, diameter was high. The heterosis of bulb firmness, skin adherence and sugar content was not high. The heterosis of other characteristics was rather low.


2023 ◽  
Vol 83 ◽  
Author(s):  
S. Masood ◽  
A. ur Rehman ◽  
M. A. Ihsan ◽  
K. Shahzad ◽  
M. Sabir ◽  
...  

Abstract Allium cepa L. is a commonly consumed vegetable that belongs to the Amaryllidaceae family and contains nutrients and antioxidants in ample amounts. In spite of the valuable food applications of onion bulb, its peel and outer fleshy layers are generally regarded as waste and exploration of their nutritional and therapeutic potential is still in progress with a very slow progression rate. The present study was designed with the purpose of doing a comparative analysis of the antioxidant potential of two parts of Allium cepa, i.g., bulb (edible part) and outer fleshy layers and dry peels (inedible part). Moreover, the inhibitory effect of the onion bulb and peel extracts on rat intestinal α-glucosidase and pancreatic α-amylase of porcine was also evaluated. The antioxidant potential of onion peel and bulb extracts were evaluated using 2,2-diphenyl- 1-picryl hydrazyl (DPPH), ferric-reducing antioxidant power assay (FRAP), 2,2’-azino-bis- 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assay, H2O2 radical scavenging activity and Fe2+ chelating activity. Total flavonoids and phenolic content of ethanolic extract of onion peel were significantly greater as compared to that of onion bulb. Ethanolic extract of onion peel also presented better antioxidant and free-radical scavenging activity as compared to the ethanolic extract of bulb, while the aqueous extract of bulb presented weakest antioxidative potential. Onion peel extract’s α-glucosidase inhibition potential was also correlated with their phenolic and flavonoid contents. The current findings presented onion peel as a possible source of antioxidative agents and phenolic compounds that might be beneficial against development of various common chronic diseases that might have an association with oxidative stress. Besides, outer dry layers and fleshy peels of onion exhibited higher phenolic content and antioxidant activities, compared to the inner bulb. The information obtained by the present study can be useful in promoting the use of vegetable parts other than the edible mesocarp for several future food applications, rather than these being wasted.


Foods ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 368
Author(s):  
Thirawat Tantamacharik ◽  
Sze Ying Leong ◽  
Michelle J. Leus ◽  
Graham T. Eyres ◽  
David J. Burritt ◽  
...  

This study investigated whether pulsed electric field (PEF) treatment can induce structural changes of whole, intact red onion bulb (Allium cepa L. var. Red Pearl). Onion bulbs were treated at electric field strengths of 0.6 and 1.2 kV/cm combined with energy inputs of 6 and 60 kJ/kg at different onion orientations with respect to the high voltage electrode. Results showed that onion cells across all fleshy scales experienced uniform cell damage with a higher proportion (>80%) of non-metabolically viable cells after PEF treatment at 1.2 kV/cm when the root end was positioned facing toward the PEF electrode. The findings were supported by cryogenic-scanning electron micrographs (cryo-SEM), where the underlying storage circular cells were completely damaged owing to the PEF treatment. In this study, it was found that the treatment intensity of PEF to induce structural damage across all the scale layers of an onion bulb coincided with an increase in dipropyl disulfide (DPDS) released from the onion bulbs. Therefore, DPDS was used as a volatile marker indicating cellular disruption within whole, intact onion bulbs. A considerable increase of DPDS, up to 52-fold, was detected from PEF-treated onion bulbs compared to untreated bulbs.


2020 ◽  
Vol 5 (2) ◽  
pp. 56-67
Author(s):  
Vera Ladeska ◽  
Rindita ◽  
Nur Amyra ◽  
Tamara Dwi Veranthy

Allium cepa L. (onion bulb) is a cultivated onion extensively and types of onions that are often used for various cuisines in Indonesia. The onion bulb was efficient in reducing blood fat levels and diuretics. This study aims to complete the onion monograph with determine the physicochemical characteristics and antioxidant activity. Microscopic results obtained include: has fibrous roots and it’s white, and about 9.5 cm long. The stem is pseudo, and the water is whitish-green, cylindrical leaves are dark green, elongated like a pipe and hollow with a length of ± 20 cm, and the tip is tapered. Onion tubers are single layer bulbs, having a diameter of 6 mm, which is higher than onions. Microscopic results contained identification fragments, including hair covering and transporting beams with thickening stairs and spirals. From the results of research on ethanol extract, 70% Allium cepa L. shows a drying shrinkage of 9.69%, 5.16% total ash content, 0.07 % acid insoluble acid dust, 14.36% water-soluble extract, and soluble ethanol extracts 23.04%. Phytochemical screening contains flavonoids, saponins, phenols, dan triterpenoids. Total flavonoid content contained in ethanol extract of onion tubers was 1.48 ± 0.12 mg QE/g. Total phenolic concentrations obtained in onion tuber ethanol extract were 103.47 ± 3.09 mg GAE/g. The antioxidant activity of ethanol extract of onion tubers by the DPPH method received IC50 is 65.31 ppm.


2009 ◽  
Vol 2009 ◽  
pp. 1-9 ◽  
Author(s):  
Masaki Fujishima ◽  
Kensuke Furuyama ◽  
Yojiro Ishihiro ◽  
Shuichi Onodera ◽  
Eri Fukushi ◽  
...  

Fructooligosaccharides are involved in physiological activities and quality attributes of onion bulbs. This work describes structures of newly synthesized oligosaccharides formed by fructose moieties in onion bulb tissues during storage. Onion bulbs were stored for four weeks at 10. HPAEC-PAD analysis showed that saccharide 1 was eluted after 1-kestose while saccharide 2 was eluted after nystose . Saccharides 1 and 2 have R-sucrose values of 1.55 and 2.15 by HPAEC, a reducing terminal, a reducing sugar-to-fructose ratio of 0.5 and 0.3, and a degree of polymerization of 2 and 3 by TOF-MS, respectively. GLC analysis of the methyl derivatives and NMR measurement of the saccharides confirmed the presence of two different structures: the structure of saccharide 1 is composed by two fructose moieties and linked by linkage and was identified as inulobiose [-D-fructofuranosyl---D-fructopyranose]. The structure of saccharide 2 consists of three units of fructose linked by linkage and was identified as inulotriose [-D-fructofuranosyl---D-fructofuranosyl---D-fructopyranose]. The spectra also showed that 70 to 80% of the terminal fructose residue of the two saccharides is of pyranosyl form, while 20 to 30% is of furanosyl form. This finding demonstrated that these newly produced saccharides, catalyzed by onion-purified 6G-FFT, were synthesized by the action of 1-FFT fructosyltransfer from 1-kestose to free fructopyranose yielding inulobiose and sucrose, while elongation of fructofuranosyl units occurs at this transferred fructofuranosyl residue to produce inulooligosaccharide having an additional unit of fructofuranose.


2010 ◽  
Vol 122 (3) ◽  
pp. 566-571 ◽  
Author(s):  
Nina Beesk ◽  
Henrike Perner ◽  
Dietmar Schwarz ◽  
Eckhard George ◽  
Lothar W. Kroh ◽  
...  

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