Sensitivity and specificity of hr hPV E6/E7 mrna test in detecting cervical squamous intraepithelial lesion and cervical cancer

Abstract Background: High-risk human papillomavirus (HR-HPV) infection is widely known as the major cause of cervical intraepithelial neoplasia (CIN) and cervical cancer and it’s characteristics vary greatly in different population. Women with abnormal cervical cytology could increase the risk of cervical cancer, however, HR-HPV infection characteristics in women with abnormal cervical cytology remains unclear. Methods: This study was based on baseline survey of the CIN Cohort established in Shanxi Province, China. A total number of 2300 women with cervical abnormalities were enrolled in this study. All participants gave informed consent and agreed to HPV and thinprepcytologic test (TCT). Each individual completed a questionnaire about characteristics related to HPV infection. Results: The overall prevalence of HR-HPV in 2300 women was 32.0%, and the proportion of single and multiple HR-HPV infections were 70.2% and 29.8% in HR-HPV infection women, respectively. The top five HR-HPV genotypes were ranked as HPV16, HPV58, HPV52, HPV53 and HPV51. The prevalence of HR-HPV in atypical squamous cells of undetermined significance (ASC-US), low-grade squamous intraepithelial lesion (LSIL) and high-grade squamous intraepithelial lesion and above(HSIL+) were 30.8%, 36.5% and 54.9%, respectively, showing an increasing trend with the severity of cervical cytology ( χ 2 trend =13.952; p <0.001). The women aged 35~45 years, with lower education level, less frequency of bathing, multiple gravidity, multiple parity, history of gynecological diseases and premenopausal women were prone to HR-HPV infection. Conclusions: We defined the characteristics related to HR-HPV infection in abnormal cervical cytology women, and provided an insight for the development and deeply research of HPV vaccine.

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Changes in the genetic landscape during the malignization of high grade squamous intraepithelial lesion into cervical cancer

Current Problems in Cancer ◽

10.1016/j.currproblcancer.2020.100567 ◽

2020 ◽

Vol 44 (5) ◽

pp. 100567

Author(s):

Nikolai V. Litviakov ◽

M.K. Ibragimova ◽

M.M. Tsyganov ◽

O.V. Shpileva ◽

O.N. Churuksaeva ◽

...

Keyword(s):

Cervical Cancer ◽

High Grade ◽

Squamous Intraepithelial Lesion ◽

Genetic Landscape ◽

Intraepithelial Lesion

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Significant decrease of a master regulator of genes (REST/NRSF) in high-grade squamous intraepithelial lesion and cervical cancer

Biomedical Journal ◽

10.1016/j.bj.2020.08.012 ◽

2020 ◽

Author(s):

Karen Cortés-Sarabia ◽

Luz del Carmen Alarcón-Romero ◽

Eugenia Flores-Alfaro ◽

Berenice Illades-Aguiar ◽

Amalia Vences-Velázquez ◽

...

Keyword(s):

Cervical Cancer ◽

High Grade ◽

Squamous Intraepithelial Lesion ◽

Master Regulator ◽

Intraepithelial Lesion

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Separation of Normal and Premalignant Cervical Epithelial Cells Using Confocal Light Absorption and Scattering Spectroscopic MicroscopyEx Vivo

Journal of Biomedicine and Biotechnology ◽

10.1155/2011/214781 ◽

2011 ◽

Vol 2011 ◽

pp. 1-5 ◽

Cited By ~ 2

Author(s):

Ling Yang ◽

Wen-Tao Liu ◽

Hao Wu ◽

Cheng Wang ◽

Bo Ping ◽

...

Keyword(s):

Light Absorption ◽

Pap Smear ◽

Precancerous Lesions ◽

Scattered Light ◽

Low Grade ◽

Squamous Intraepithelial Lesion ◽

Cervical Lesions ◽

Pap Smear Test ◽

Intraepithelial Lesion ◽

Cervical Squamous Intraepithelial Lesion

Confocal light absorption and scattering spectroscopic (CLASS) microscopy can detect changes in biochemicals and the morphology of cells. It is therefore used to detect high-grade cervical squamous intraepithelial lesion (HSIL) cells in the diagnosis of premalignant cervical lesions. Forty cervical samples from women with abnormal Pap smear test results were collected, and twenty cases were diagnosed as HSIL; the rest were normal or low-grade cervical squamous intraepithelial lesion (LSIL). The enlarged and condensed nuclei of HSIL cells as viewed under CLASS microscopy were much brighter and bigger than those of non-HSIL cells. Cytological elastic scattered light data was then collected at wavelengths between 400 and 1000 nm. Between 600 nm to 800 nm, the relative elastic scattered light intensity of HSIL cells was higher than that of the non-HSIL. Relative intensity peaks occurred at 700 nm and 800 nm. CLASS sensitivity and specificity results for HSIL and non-HSIL compared to cytology diagnoses were 80% and 90%, respectively. This study demonstrated that CLASS microscopy could effectively detect cervical precancerous lesions. Further study will verify this conclusion before the method is used in clinic for early detection of cervical cancer.

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Treatment of high risk human papillomavirus infection in low grade cervical squamous intraepithelial lesion with mild local thermotherapy

Medicine ◽

10.1097/md.0000000000021005 ◽

2020 ◽

Vol 99 (27) ◽

pp. e21005 ◽

Cited By ~ 1

Author(s):

Yang Yang ◽

Lan Zhang ◽

Ruiqun Qi ◽

Wei Huo ◽

Xiaodong Li ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Low Grade ◽

Squamous Intraepithelial Lesion ◽

Human Papillomavirus Infection ◽

Papillomavirus Infection ◽

Intraepithelial Lesion ◽

Cervical Squamous Intraepithelial Lesion

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Spectrum of cytological patterns in cervical PAP smears in a tertiary care center of Western region of Nepal

Nepal Journal of Medical Sciences ◽

10.3126/njms.v4i1.24118 ◽

2019 ◽

Vol 4 (1) ◽

pp. 2-8

Author(s):

Pragya Gautam Ghimire ◽

Durga BC Rawat ◽

Kavita Sinha ◽

Kamar Jahan ◽

Richa Shrestha

Keyword(s):

Cervical Cancer ◽

Epithelial Cell ◽

Pap Smear ◽

Statistical Significance ◽

Care Center ◽

Tertiary Care ◽

Pap Smears ◽

Low Grade ◽

Squamous Intraepithelial Lesion ◽

Intraepithelial Lesion

Introduction: Cervical cancer is a common health problem in Nepal. There is paucity of data regarding the spectrum of findings in cervical Pap in western Nepal. This study was aimed to study the cytological patterns in cervical Pap smears in patients in a tertiary hospital of Nepal. Methods: This is a prospective, cross sectional, hospital based study. Clinical features of patients who had presented with Pap smear was noted in a structured proforma. Pap smears were studied by a senior pathologist and reported based on revised Bethesda system (2014). Results: Most of the cases belonged to 31-40 years 399 (42.8%). Unsatisfactory/ inadequate sample was present in 133(14.05%) with obscuration due to inflammatory exudate being most common cause. Negative for intraepithelial lesion or malignancy rate was noted in 798 (85.54%) with 477(51.2%) being normal findings. Epithelial cell abnormalities were noted in 116 (14.5 %) smears. Low-grade squamous intraepithelial lesion constituted 321(34.5%), High grade squamous intraepithelial lesion 273(29.3 %) and Atypical squamous cells of undetermined significance 153(16.4%) of epithelial cell abnormalities. Squamous cell carcinoma was present in 9(1%) of all reviewed smears. There was no statistical significance between the age and abnormalities of Pap smear (p=0.9). Conclusions: Pap smear is pivotal in cervical cancer screening in developing countries. It also identifies various inflammatory, infective, benign and malignant pathologies at the earliest thereby decreasing the morbidity and mortality.

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Underlying pathology of women with "atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion" smears, in a region with a high incidence of cervical cancer

Journal of Obstetrics and Gynaecology Research ◽

10.1111/j.1447-0756.2008.00758.x ◽

2008 ◽

Vol 34 (2) ◽

pp. 204-209 ◽

Cited By ~ 13

Author(s):

Chumnan Kietpeerakool ◽

Jatupol Srisomboon ◽

Charuwan Tantipalakorn ◽

Prapaporn Suprasert ◽

Surapan Khunamornpong ◽

...

Keyword(s):

Cervical Cancer ◽

High Grade ◽

Squamous Intraepithelial Lesion ◽

Squamous Cells ◽

High Incidence ◽

Intraepithelial Lesion ◽

Underlying Pathology

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Cervical pap smear- A prospective study in a tertiary hospital

Journal of Pathology of Nepal ◽

10.3126/jpn.v5i10.15639 ◽

2015 ◽

Vol 5 (10) ◽

pp. 820-823 ◽

Cited By ~ 2

Author(s):

S Pudasaini ◽

KBR Prasad ◽

SK Rauniyar ◽

R Pathak ◽

K Pande ◽

...

Keyword(s):

Cervical Cancer ◽

Pap Smear ◽

Cost Effective ◽

Tertiary Hospital ◽

Pap Smears ◽

Low Grade ◽

Squamous Intraepithelial Lesion ◽

Effective Screening ◽

Intraepithelial Lesion ◽

A Prospective Study

Background: Cervical cancer is a leading cause of mortality and morbidity among women worldwide and most common gynaecological cancer in developing countries. Papanicolaou smear is a simple and cost effective screening test for cervical cancer. The aim of this study is to evaluate and interpret the cervical pap smear cytology in a tertiary hospital. The interpretation and reporting of the pap smear is based on 2001Bethesda system.Materials and methods: This is a prospective study conducted in a tertiary hospital, Nepal Medical College over a period of two and a half years (January 2013 to June 2015). All cervical pap smears received in the department of Pathology in the study period were included.Results: A total of 4160 cervical pap smears were reported in the study period. Majority of the cases were Negative for Intraepithelial lesion or malignancy (87.9%). Bacterial vaginosis, atrophy and reactive cellular changes associated with inflammation were seen in 5.3%, 2.4% and 1.5% cases respectively. Epithelial cell abnormalities (0.5%) include Atypical squamous cells of undetermined significance, Low grade squamous intraepithelial lesion and High grade intraepithelial lesion. 88% of Low grade squamous intraepithelial lesion was seen in reproductive age group (20-45 years).Conclusion: Cervical cancer is the most common gynaecological cancer in the developing countries. Pap smear is the simple and cost effective screening tool to detect pre invasive cervical epithelial lesions.

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