scholarly journals The Anti-Cancer Effects of Anti-Parasite Drug Ivermectin in Ovarian Cancer

2021 ◽  
Author(s):  
Xianquan Zhan ◽  
Na Li
Keyword(s):  
Ovarian Cancer ◽  
Energy Metabolism ◽  
Rna Binding ◽  
Lasso Regression ◽  
Clinicopathologic Characteristics ◽  
Pathway Network ◽  
Prognostic Assessment ◽  
Molecular Pattern ◽  
Anti Cancer ◽  
Block Cell Cycle Progression

Ivermectin is an old, common, and classic anti-parasite drug, which has been found to have a broad-spectrum anti-cancer effect on multiple human cancers. This chapter will focus on the anti-cancer effects of ivermectin on ovarian cancer. First, ivermectin was found to suppress cell proliferation and growth, block cell cycle progression, and promote cell apoptosis in ovarian cancer. Second, drug pathway network, qRT-PCR, and immunoaffinity blot analyses found that ivermectin acts through molecular networks to target the key molecules in energy metabolism pathways, including PFKP in glycolysis, IDH2 and IDH3B in Kreb’s cycle, ND2, ND5, CYTB, and UQCRH in oxidative phosphorylation, and MCT1 and MCT4 in lactate shuttle, to inhibit ovarian cancer growth. Third, the integrative analysis of TCGA transcriptomics and mitochondrial proteomics in ovarian cancer revealed that 16 survival-related lncRNAs were mediated by ivermectin, SILAC quantitative proteomics analysis revealed that ivermectin extensively inhibited the expressions of RNA-binding protein EIF4A3 and 116 EIF4A3-interacted genes including those key molecules in energy metabolism pathways, and also those lncRNAs regulated EIF4A3-mRNA axes. Thus, ivermectin mediated lncRNA-EIF4A3-mRNA axes in ovarian cancer to exert its anticancer capability. Further, lasso regression identified the prognostic model of ivermectin-related three-lncRNA signature (ZNRF3-AS1, SOS1-IT1, and LINC00565), which is significantly associated with overall survival and clinicopathologic characteristics in ovarian cancer patients. These ivermectin-related molecular pattern alterations benefit for prognostic assessment and personalized drug therapy toward 3P medicine practice in ovarian cancer.

scholarly journals Propofol suppresses cell viability, cell cycle progression and motility and induces cell apoptosis of ovarian cancer cells through suppressing MEK/ERK signaling via targeting circVPS13C/miR-145 axis

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Huan Lu ◽  
Guanlin Zheng ◽  
Xiang Gao ◽  
Chanjuan Chen ◽  
Min Zhou ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Ovarian Cancer ◽  
Cancer Cells ◽  
Rna Binding ◽  
Erk Signaling ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Ovarian Cancer Cells ◽  
Dependent Manner ◽  
Vacuolar Protein

Abstract Background Propofol is a kind of common intravenous anaesthetic agent that plays an anti-tumor role in a variety of cancers, including ovarian cancer. However, the working mechanism of Propofol in ovarian cancer needs further exploration. Methods The viability and metastasis of ovarian cancer cells were assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and transwell assays. Flow cytometry was used to evaluate the cell cycle and apoptosis. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to examine the abundance of circular RNA vacuolar protein sorting 13 homolog C (circVPS13C) and microRNA-145 (miR-145). The target relationship between miR-145 and circVPS13C was predicted by circinteractome database and verified by dual-luciferase reporter assay, RNA-binding protein immunoprecipitation (RIP) assay and RNA-pull down assay. Western blot assay was used to detect the levels of phosphorylated extracellular regulated MAP kinase (p-ERK), ERK, p-MAP kinse-ERK kinase (p-MEK) and MEK, in ovarian cancer cells. Results Propofol treatment suppressed the viability, cell cycle and motility and elevated the apoptosis rate of ovarian cancer cells. Propofol up-regulated miR-145 in a dose-dependent manner. Propofol exerted an anti-tumor role partly through up-regulating miR-145. MiR-145 was a direct target of circVPS13C. Propofol suppressed the progression of ovarian cancer through up-regulating miR-145 via suppressing circVPS13C. Propofol functioned through circVPS13C/miR-145/MEK/ERK signaling in ovarian cancer cells. Conclusion Propofol suppressed the proliferation, cell cycle, migration and invasion and induced the apoptosis of ovarian cancer cells through circVPS13C/miR-145/MEK/ERK signaling in vitro.

scholarly journals Low-grade serous epithelial ovarian cancer: a comprehensive review and update for radiologists

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sofia Amante ◽  
Filipa Santos ◽  
Teresa Margarida Cunha
Keyword(s):  
Ovarian Cancer ◽  
Serous Carcinoma ◽  
Low Grade ◽  
Resonance Imaging ◽  
Clinicopathologic Characteristics ◽  
Borderline Tumour ◽  
Molecular Features ◽  
Serous Epithelial Ovarian Cancer ◽  
The Difference

AbstractLow-grade serous carcinoma (LGSC) is an infrequent subtype of ovarian cancer, corresponding to 5% of epithelial neoplasms. This subtype of ovarian carcinoma characteristically has molecular features, pathogenesis, clinical behaviour, sensitivity to chemotherapy, and prognosis distinct to high-grade serous carcinoma (HGSC). Knowing the difference between LGSC and other ovarian serous tumours is vital to guide clinical management, which currently is only possible histologically. However, imaging can provide several clues that allow differentiating LGSC from other tumours and enable precise staging and follow-up of ovarian cancer treatment. Characteristically, LGSC appears as mixed lesions with variable papillary projections and solid components, usually in different proportions from those detected in serous borderline tumour and HGSC. Calcified extracellular bodies, known as psammoma bodies, are also a common feature of LGSC, frequently detectable within lymphadenopathies and metastases associated with this type of tumour. In addition, the characterisation of magnetic resonance imaging enhancement also plays an essential role in calculating the probability of malignancy of these lesions. As such, in this review, we discuss and update the distinct radiological modalities features and the clinicopathologic characteristics of LGSC to allow radiologists to be familiarised with them and to narrow the differential diagnosis when facing this type of tumour.

A mechanistic study on the anti-cancer activity of ethyl caffeate in human ovarian cancer SKOV-3 cells

2014 ◽  
Vol 219 ◽  
pp. 151-158 ◽  
Author(s):  
Ha Neul Lee ◽  
Jin-Kyu Kim ◽  
Jae Hyeon Kim ◽  
Sang-Jin Lee ◽  
Eun-Kyung Ahn ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Mechanistic Study ◽  
Human Ovarian Cancer ◽  
Anti Cancer ◽  
Cancer Activity

scholarly journals The RNA-binding protein LARP1 is a post-transcriptional regulator of survival and tumorigenesis in ovarian cancer

2015 ◽  
Vol 44 (3) ◽  
pp. 1227-1246 ◽  
Author(s):  
Thomas G. Hopkins ◽  
Manuela Mura ◽  
Hiba A. Al-Ashtal ◽  
Roni M. Lahr ◽  
Normala Abd-Latip ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Transcriptional Regulator

scholarly journals Development and Validation an Epigenetic Modification-related Signals for Diagnosis and Prognosis of Hepatocellular Carcinoma

2021 ◽  
Author(s):  
Diguang Wen ◽  
Sheng Qiu ◽  
Zuojin Liu
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cancer ◽  
Rna Binding ◽  
Cox Regression ◽  
Rna Binding Proteins ◽  
Epigenetic Modification ◽  
Lasso Regression ◽  
Cancer Dataset ◽  
Gene Signatures ◽  
Diagnosis And Prognosis

Abstract Background: Increasing evidence has indicated that abnormal epigenetic modification such as RNAm6a modification, histone modification, DNA methylation modification, RNA binding proteins and transcription factors, is correlated with Hepatocarcinogenesis. However, it is unknown how epigenetic modification associated genes contribute to the occurrence and clinical outcome of hepatocellular carcinoma (HCC). Thus, we constructed epigenetic modification associated model that may enhance the diagnosis and prognosis of HCC.METHODS: In this study, we focused on the clinical values of epigenetic modification associated genes for HCC. Our gene expression data were collected from TCGA and a HCC datasets from GEO dataset in order to ensure the reliability of data. Their function was analyzed by bioinformatics methods. We used lasso regression, SUV, logistic regression and cox regression to construct the diagnosis and prognosis models. We also constructed a nomogram for the practicability of the above-mentioned prognosis model. The above results have been verified in an independent liver cancer dataset from ICGC database. Furthermore, we carried out pan cancer analysis to verify the specificity of the above model.RESULT: A large number of epigenetic modification associated genes were significantly different in HCC and normal liver tissues. The gene signatures showed good performance for predicting the occurrence and survival of HCC patients verified by DCA and ROC curve.CONCLUSION: Gene signatures based on epigenetic modification associated genes can be used to identify the occurrence and prognosis of liver cancer.

scholarly journals Proline-rich protein 11 overexpression is associated with a more aggressive phenotype and poor overall survival in ovarian cancer patients

2020 ◽  
Author(s):  
yu zhan ◽  
xueyuan wu ◽  
gang zheng ◽  
jingjing jin ◽  
chaofu li ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Overall Survival ◽  
Curative Surgery ◽  
Poor Overall Survival ◽  
Expression Levels ◽  
Prognostic Assessment ◽  
Therapy Strategies ◽  
Proline Rich Protein

Abstract Background: The proline-rich protein 11 (PRR11) is a newly identified oncogene associated with a poor prognosis in several human cancers. Nonetheless, research on its role in ovarian cancer (OC) remains largely understudied. Therefore, this study aims to evaluate the expression levels of PRR11 protein and its role in human ovarian cancer. Methods: Immunohistochemistry analysis was used to evaluate the expression levels of PRR11 protein in human samples obtained from 49 patients diagnosed with OC and subjected to curative surgery in the First Affiliated Hospital of Wenzhou Medical University between 2007 and 2015. Results: In total, 57.1% of the primary OC tumor tissue evaluated demonstrated overexpression of PRR11. Meanwhile, survival analysis showed that the overall survival (OS) of patients presenting overexpression of PRR11 was significantly lower than the OS of the patients with negative PRR11. In subsequent experiments, it was found that silencing the expression of PRR11 expression inhibited the proliferation of tumor cells and the migration of cells in vitro. Further, cells subjected to PRR11 knockdown exhibited a decrease in tumor growth in vivo. The downregulation of PRR11 was coupled with a decrease in N-cadherin and downregulation in the expression of early growth response protein 1 (EGR1).Conclusions: The findings suggest that PRR11 might be considered as a potential target for prognostic assessment and gene therapy strategies for patients diagnosed with OC.

scholarly journals Hemp Extract with Specific Anti‐Cancer Properties against Ovarian Cancer

2021 ◽  
Vol 35 (S1) ◽  
Author(s):  
Wasana Sumanasekera ◽  
Adam Duff ◽  
Sara Biela ◽  
Annie Wang ◽  
Kayla Riggs ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Anti Cancer
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