scholarly journals Bacillus megaterium Biodegradation Glyphosate

2021 ◽  
Author(s):  
Nibal Khaleel Mousa ◽  
Abdul-Jabbar Ali ◽  
Maha Hussein
Keyword(s):  
Incubation Period ◽  
Bacillus Megaterium ◽  
Organophosphorus Pesticides ◽  
Nitrogen Sources ◽  
Mineral Salt ◽  
Morphological Identification ◽  
Biochemical Tests ◽  
Carbon And Nitrogen ◽  
Degradation Ratio ◽  
Glyphosate Degradation

The Bacillus megaterium ability was evaluated in this paper to degrade the Glyphosate. organophosphorus pesticides, The bacteria re-cultured that isolated from other researches of Baghdad soils and morphological identification and biochemical tests besides by selectivity media. The (5 and 25) ppm showed the highest growth results were within two days to two months on mineral salt media. The highest glyphosate degradation ratio % were (70) % per 25 ppm/two months. Incubation period Increasing led to highest glyphosate degradation ratio% at (25) ppm led to conclusion that bacteria digestive the pesticides as carbon and nitrogen sources and will be well harvest it form contaminated areas.

scholarly journals Optimum Conditions for Phenol Oxidase (Laccase) Production from Azotobacter chroococcum by Submerged Fermentation

2017 ◽  
Vol 11 (1) ◽  
pp. 13-18
Author(s):  
Ali J. R. AL-Sa'ady ◽  
Lamees M.R. Abbas ◽  
Hutaf A.A. ALsalim
Keyword(s):  
Incubation Period ◽  
Incubation Temperature ◽  
Nitrogen Sources ◽  
Phenol Oxidase ◽  
Azotobacter Chroococcum ◽  
Laccase Production ◽  
Optimum Conditions ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Production Conditions

Phenol oxidases (laccase), copper-containing oxidase enzymes, are found in many plants, fungi and microorganisms. Many factors that influence laccase production from Azotobacter chroococcum are determined in this study, these factors are: carbon source, nitrogen source, pH, incubation temperature and incubation period. The results showed that the best carbon and nitrogen sources for laccase production are sucrose and yeast extract respectively, and the best factors for laccase production conditions are pH 7.0 with an incubation period of 6 days at temperature 30 Cᵒ.

scholarly journals Caseinase Production and Media Optimization from Bacillus subtilis

2020 ◽  
Vol 71 (11) ◽  
pp. 1-9
Author(s):  
Noor Nihad Abdul Hussein ◽  
Aseel I. Ibrahim ◽  
Firas Hashim Kamar ◽  
Arelia Cristina Nechifor
Keyword(s):  
Bacillus Subtilis ◽  
Enzyme Production ◽  
Paper Industry ◽  
Nitrogen Sources ◽  
The Body ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Sequencing Result

Caseinase is involved in the breakdown of milk protein casein and converts casein into smaller simple sugars which can be easily utilized by the body for the production of ATP and Fat. Casein can be an instant energy source to the body and involves in muscle building. Caseinase enzyme can be extensively used at the industrial scale for Milk, Textile, Dairy, Paper industry and several other medical purposes. In view of the importance of caseinase, the current research deals with the isolation and identification of caseinase producing bacteria from soil. This is followed by the production of enzyme and its purification. The study also includes its kinetic characterization using the parameters Temperature, pH as well as Carbon and Nitrogen Sources. The organism which was isolated from soil and capable of producing the caseinase enzyme was identified to be Bacillus subtilis based on the Biochemical tests and 16S rRNA sequencing result. The optimal carbon and nitrogen sources were identified to be Glucose and casein respectively. Regarding the optimal conditions, the suitable temperature for maximum enzyme production was found to be 40 0C and pH was 9. When the organism was cultured under the optimal condition using casein as a nitrogen source and glucose as the carbon source, at 40 0C and pH 9, 1590 ng/mL of enzyme production was estimated.

scholarly journals BACILLUS MEGATERIUM BIODEGRADATION GLYCOPHATE

2019 ◽  
Vol 50 (6) ◽  
Author(s):  
Mousa & et al.
Keyword(s):  
Bacillus Megaterium ◽  
Degradation Rate ◽  
Organophosphorus Pesticides ◽  
Contaminated Sites ◽  
Biochemical Tests

This study was aimed to evaluate the Bacillus megatrium ability to growth and degradated the organophosphorus pesticides, Glyphosate. , Bacillus megaterium was isolated from Iraqi Soils and identification by morphological and biochemical tests beside a Sperber’s Medium as selectivity media. The best growth results were in (2- 60) days, had the same growth for both (5, 25) ppm on MSM. The best degradation rate ability % were in (25) ppm /60 days (70.9)%. The increasing in incubation show increasing of degradation ration% of Glyphosate  via HPLC specially after 60 days , the best ration were for (25)ppm .The result is the B. megaterium used the Glyphosate as source for carbon and phosphorus and suggest could be well exploited for bioremediation of Glyphosate contaminated sites.

scholarly journals Ability of the fungus Aureobasidium pullulans to produce polysaccharide (pullulan) with presence of different Carbon and Nitrogen sources

2014 ◽  
Vol 8 (1) ◽  
pp. 67-70
Author(s):  
Zahraa Ibraheem Muhammed Al_dabbagh ◽  
Muhammed Basheer Ismaeil Kassim
Keyword(s):  
Amino Acid ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Incubation Period ◽  
Aureobasidium Pullulans ◽  
Nitrogen Sources ◽  
Extracellular Polysaccharide ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Amino Acid Glycine

The effect of incubation period, different carbon and nitrogen sources on the production of the extracellular polysaccharide pullulan by Aureobasidium pullulansNRRL58560 was examined. The results showed that the maximum production of pullulan was obtained 13.76 after 96 hours of incubation. Sucrose as a carbon source gave the highest production of pullulan 31.92 g/l while the highest biomass was obtained when starch was used as a carbon source 16.30 g/l. The amino acid glycine as a nitrogen source gave the highest production of pullulan 34.61 g/l and the highest production of biomass 14.49 g/l was obtained when ammonium chloride was used.

scholarly journals Production, Purification and Optimisation of Amylase by Submerged Fermentation Using Bacillus Subtilis

2019 ◽  
pp. 265-275
Author(s):  
Aastha Acharya ◽  
Amit Khanal ◽  
Mrishal Ratna Bajracharya ◽  
Aashish Timalsina ◽  
Arjun Bishwokarma ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Maximum Yield ◽  
Cell Immobilization ◽  
Nitrogen Sources ◽  
Biochemical Tests ◽  
Carbon And Nitrogen ◽  
Nutrient Agar Medium ◽  
Amylase Production ◽  
Bacillus Spp ◽  
Almost All

Amylase is abundantly present in nature. The main source of this enzyme is the microbial origin. It is known that about two-third of the industrial enzymes (amylase, protease, cellulose, penicillinase, chitinase, etc,) are produced by the Bacillus spp. The present work comprised in the identification of amylase producing Bacillus spp and exposure of the producers to various parameters for the maximum yield of the enzyme. To isolate and identify the amylase and protease producing strain, soil samples were collected from different vegetation from the altitude at 4367.35 feet above sea level. The isolates were screened and various biochemical tests and morphological observations were done to identify the isolates. The enzymes were produced by the submerged state fermentation (SmF) from the isolates and purified by dialysis. Effects of temperature, pH, and different carbon and nitrogen sources of the medium using SmF were optimized. Among 95 isolates, 36 were identified. Among the identified isolates, Bacillus subtilis produced the maximum yield and thus, it was optimized for the amylase production. The maximum amylase production was found at 42 C temperature, in fructose as a carbon sugar, peptone as a nitrogen source and at pH 7. Almost all the enzyme producers inhabited the roots of leguminous plants. In the present study, starch is used with the nutrient agar medium to help in cell immobilization for maximum production of amylase by strains of Bacillus. More sophisticated process of purification such as chromatography and electrophoresis will yield more enzyme as compared to the dialysis.

Isolation and Characterization of Three Bacterium Strains for Biodegradation of Methamidophos

2014 ◽  
Vol 955-959 ◽  
pp. 235-238 ◽  
Author(s):  
Dan Dan Li ◽  
Yong Ze Yuan ◽  
Yuan Lei Chen ◽  
Na Li ◽  
Jing Long Li ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Organophosphorus Pesticides ◽  
Inorganic Nitrogen ◽  
Morphological Characteristics ◽  
Pseudomonas Stutzeri ◽  
Carbon And Nitrogen ◽  
16S Rdna Sequence Analysis ◽  
Isolation And Characterization ◽  
Physiological Properties ◽  
Degradation Ratio

In this study, three bacterium strains capable of degrading methamidophos (MAP) were isolated from the soil samples contaminated with organophosphorus pesticides (OPs). According to morphological characteristics, physiological properties, and 16S rDNA sequence analysis, the isolates HS-A32, HS-D36, and HS-D38 were identified asAcinetobacter sp.,Pseudomonas stutzeriandPseudomonas aeruginosa, respectively. They could utilize MAP as the sole carbon and nitrogen source, and the degradation ratio upon 500 mg/L MAP could reach 80% in 3 days under optimal conditions (pH 7.0, 30-35 °C). The strains could use glucose, fructose, ethanol, and galactose as carbon and energy sources, and the favorable nitrogen source included organic and inorganic nitrogen. Further studies showed that the three strains could degrade various OPs. The characterized strains with broad-spectrum OP-degrading activities could be useful for the biodegradation of MAP and the other OPs.

Sign in / Sign up

Export Citation Format

Share Document