scholarly journals Metformin for Tuberculosis Infection

2021 ◽  
Author(s):  
Bernadette Dian Novita ◽  
Ari Christy Mulyono ◽  
Ferdinand Erwin
Keyword(s):  
Reactive Oxygen Species ◽  
Superoxide Dismutase ◽  
Mycobacterium Tuberculosis ◽  
Treatment Failure ◽  
Tissue Damage ◽  
Antioxidant Defense System ◽  
Resistance Rate ◽  
Defense System ◽  
Macrophage Activity ◽  
Activity Inhibition

Tuberculosis, caused by Mycobacterium tuberculosis (M.tb), remains the biggest infection burden in the word. Rifampin (RIF) and Isoniazid (INH) are the most effective antibiotics for killing M.tb. However, the resistance rate of rifampin and INH are high and lead to almost 35% treatment failure. Metformin enhanced anti tuberculosis efficacy in killing M. tuberculosis through several mechanism, firstly through autophagia mechanism and secondly by activating superoxide dismutase (SOD). Metformin activated mTOR and AMPK then induced more effective autophagy against M.tb. Superoxide Dismutase (SOD) is an enzyme produced in the host’s antioxidant defense system. SOD neutralizes reactive oxygen species (ROS) that excessively produced during phagocytosis process against M.tb. Excessive production of ROS associated with Th1 overactivation and leads into macrophage activity inhibition and excessive tissue damage. Metformin has ability in improving SOD level during inflammation.

scholarly journals Corrective effect of red wine concentrate enriched with natural complex of polyphenols on activity of antioxidant defense enzymes in cardiac muscle under experimental diabetes mellitus

2021 ◽  
Vol 15 (1) ◽  
pp. 37-48
Author(s):  
D. Yu. Hertsyk ◽  
◽  
M. V. Sabadashka ◽  
L. V. Kaprelyants ◽  
N. O. Sybirna ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Superoxide Dismutase ◽  
Antioxidant Defense ◽  
Red Wine ◽  
Experimental Diabetes ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Experimental Diabetes Mellitus ◽  
Corrective Effect ◽  
Antioxidant Defense Enzymes

Background. Diabetes mellitus is accompanied by oxidative-nitrative stress, which is caused both by an increased generation of the Reactive Oxygen Species and the Reactive Nitrogen Species under the conditions of this pathology and by the disorders of the antioxidant defense system, especially its enzymatic part. This leads alterations in the morpho-functional state of cells, organs, and the whole organism. Free radicals are involved in the destruction of pancreatic cells in type 1 diabetes mellitus, which leads to even greater inhibition of insulin secretion, worsening of the course of the disease and occurrence of diabetic complications. The complications of diabetes mellitus include often diagnosed cardiovascular diseases. Therefore, the use of treatments characterized not only by hypoglycemic properties for normalization of blood sugar level in diabetes mellitus, but also by antioxidant properties for normalization of oxidative/antioxidant balance of the organism in the studied pathology can be promising. Scientists pay a great attention to the study of substances with such properties, especially natural phenolic compounds of grape, which are also characterized by immunomodulatory properties. Materials and Methods. The study was performed on cardiac tissues of control rats, control animals treated with wine polyphenolic complex concentrate, animals with streptozotocin-induced diabetes mellitus and animals with experimental diabetes mellitus treated with a concentrate of wine polyphenolic complex. The activity of catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase were detected to examine the corrective effect of the concentrate of red wine natural polyphenolic comp­lex on the state of the enzymatic part of the antioxidant defense system. Results. The results have shown the normalization of activity of catalase, superoxide dismutase, and changes in the activity of enzymes of glutathione cycle after oral administration of polyphenolic complex concentrate for 14 days to rats with streptozo­tocin-induced diabetes mellitus. Conclusions. The results confirm a hypothesis about the antioxidant effect of the studied concentrate and the ability of natural polyphenolic complexes to serve as the basis for new drugs for treatment of diabetes-induced disorders.

Oxidative Stress in Critically Ill Patients

2002 ◽  
Vol 11 (6) ◽  
pp. 543-551 ◽  
Author(s):  
Caryl Goodyear-Bruch ◽  
Janet D. Pierce
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Superoxide Dismutase ◽  
Free Radicals ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Oxygen Free Radicals ◽  
Oxygen Species ◽  
Defense System ◽  
Oxygen Derived Free Radicals

Oxygen-derived free radicals play an important role in the development of disease in critically ill patients. Normally, oxygen free radicals are neutralized by antioxidants such as vitamin E or enzymes such as superoxide dismutase. However, in patients who require intensive care, oxygen free radicals become a problem when either a decrease in the removal or an overproduction of the radicals occurs. This oxidative stress and the damage due to it have been implicated in many diseases in critically ill patients. Many drugs and treatments now being investigated are directed toward preventing the damage from oxidative stress. The formation of reactive oxygen species, the damage caused by them, and the body’s defense system against them are reviewed. New interventions are described that may be used in critically ill patients to prevent or treat oxidative damage.

scholarly journals Molecular Mechanism of Heavy Metal Toxicity and Tolerance in Plants: Central Role of Glutathione in Detoxification of Reactive Oxygen Species and Methylglyoxal and in Heavy Metal Chelation

2012 ◽  
Vol 2012 ◽  
pp. 1-37 ◽  
Author(s):  
Mohammad Anwar Hossain ◽  
Pukclai Piyatida ◽  
Jaime A. Teixeira da Silva ◽  
Masayuki Fujita
Keyword(s):  
Reactive Oxygen Species ◽  
Heavy Metal ◽  
Antioxidant Defense ◽  
Plant Cells ◽  
Reactive Oxygen ◽  
Antioxidant Defense System ◽  
Peroxidation Of Lipids ◽  
Oxygen Species ◽  
Defense System ◽  
Glyoxalase System

Heavy metal (HM) toxicity is one of the major abiotic stresses leading to hazardous effects in plants. A common consequence of HM toxicity is the excessive accumulation of reactive oxygen species (ROS) and methylglyoxal (MG), both of which can cause peroxidation of lipids, oxidation of protein, inactivation of enzymes, DNA damage and/or interact with other vital constituents of plant cells. Higher plants have evolved a sophisticated antioxidant defense system and a glyoxalase system to scavenge ROS and MG. In addition, HMs that enter the cell may be sequestered by amino acids, organic acids, glutathione (GSH), or by specific metal-binding ligands. Being a central molecule of both the antioxidant defense system and the glyoxalase system, GSH is involved in both direct and indirect control of ROS and MG and their reaction products in plant cells, thus protecting the plant from HM-induced oxidative damage. Recent plant molecular studies have shown that GSH by itself and its metabolizing enzymes—notably glutathione S-transferase, glutathione peroxidase, dehydroascorbate reductase, glutathione reductase, glyoxalase I and glyoxalase II—act additively and coordinately for efficient protection against ROS- and MG-induced damage in addition to detoxification, complexation, chelation and compartmentation of HMs. The aim of this review is to integrate a recent understanding of physiological and biochemical mechanisms of HM-induced plant stress response and tolerance based on the findings of current plant molecular biology research.

scholarly journals ACTIVITY OF MITOCHONDRIAL ANTIOXIDANT DEFENSE SYSTEM IN YOUNG PATIENTS WITH GASTROESOPHAGEAL REFLUX DISEASE

2020 ◽  
Vol 7 (4) ◽  
pp. 164-167
Author(s):  
Olga Kovalyova ◽  
Tamara Pasiieshvili
Keyword(s):  
Superoxide Dismutase ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Reflux Disease ◽  
Manganese Superoxide Dismutase ◽  
Antioxidant Defense ◽  
Young Patients ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Manganese Superoxide

Background. Despite numerous studies, the pathogenesis of gastroesophageal reflux disease remains unclear. Aim of research: assessment the activity of antioxidant defense system in young patients with GERD based on expression of biomarker associated with mitochondrial function. Material and methods. The study included 45 patients with gastroesophageal reflux disease. The examined contingent was presented by students age from 18 to 25 years. 20 healthy persons were included as control group. Levels of manganese superoxide dismutase were determined in blood serum of study persons with enzyme immunoassays (ELISA, Elabscience, USA). Statistical data processing by the Statistica Basic Academic 13 for Windows En local was made. Results. Gastroesophageal reflux disease in young patients is characterized by significantly increasing of manganese superoxide dismutase as compare to control group (7.1700 ng/ml vs 4.4720 ng/ml respectively, p<0.01). Presence of erosion in esophagus mucous doesn't accompanied by significant changes of evaluated parameter as compare with non-erosion form of disease in patients. Conclusion. The elevation in young patients with GERD the biomarker of mitohondrial antioxidant defense system we may speculate as adaptive response contributing to non-specific citoprotection. Taking to account the publishing facts about dual role of manganese superoxide dismutase it is necessary to monitoring antioxidant enzyme in patients with gastroesophageal reflux disease for prediction of possible complications and outcome.

scholarly journals Increased Activity of Hippocampal Antioxidant Enzymes as an Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats

2017 ◽  
Vol 67 (4) ◽  
pp. 540-550 ◽  
Author(s):  
Nataša Popović ◽  
B. Snežana Pajović ◽  
Vesna Stojiljković ◽  
Ana Todorović ◽  
Snežana Pejić ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Manganese Superoxide Dismutase ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Copper Zinc Superoxide Dismutase ◽  
Protein Levels ◽  
Copper Zinc ◽  
Increased Activity

AbstractThis study examined the effects of chronic restraint stress (CRS: 2 hours × 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide dismutase (SOD 1), manganese superoxide dismutase (SOD 2) and catalase (CAT) in the rat hippocampus. Also, we examined changes in the activities of SOD 1, SOD 2 and CAT in the hippocampus of chronically stressed rats. Investigated parameters were quantifi ed by using real-time RT-PCR, Western blot analysis and assay of enzymatic activity. We found that CRS did not change mRNA and protein levels of SOD 1 and CAT, but increased mRNA and protein levels of SOD 2. However, CRS treatment increased the enzyme activities of SOD 1, SOD 2 and CAT. Our fi ndings indicate that the increased activity of antioxidant enzymes (SOD 1, SOD 2 and CAT) in the hippocampus may be an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats.

scholarly journals Characteristics of lipid peroxidation processes and factors of the antioxidant defense system in chronic atrophic gastritis and gastric cancer

2022 ◽  
Vol 20 (4) ◽  
pp. 63-70
Author(s):  
O. V. Smirnova ◽  
V. V. Tsukanov ◽  
A. A. Sinyakov ◽  
O. L. Moskalenko ◽  
N. G. Elmanova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gastric Cancer ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Control Group ◽  
Defense System ◽  
Glutathione S Transferase

Background. The problem of gastric cancer remains unresolved throughout the world, while chronic atrophic gastritis (CAG) increases the likelihood of its development by 15 times. In the Russian Federation, the incidence of gastric cancer (GC) is among the highest, with it prevailing among males. One of the leading mechanisms in molecular pathology of membranes is lipid peroxidation (LPO). The severity of oxidative membrane damage depends on concomitant diseases, contributing to emergence and progression of pathological processes and development of cancer. Currently, the problem of LPO is unsolved in biological systems.The aim of this study was to investigate the state of LPO and antioxidant defense system in CAG and GC. Materials and methods. The parameters were studied in 45 patients with CAG and 50 patients with GC. The control group included 50 practically healthy volunteers without gastrointestinal complaints, who did not have changes in the gastric mucosa according to the fibroesophagogastroduodenoscopy (FEGDS) findings.Results. In patients with CAG, an increase in malondialdehyde, superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase was found in the blood plasma compared with the control group. In patients with CAG, lipid peroxidation was activated, and the malondialdehyde level increased by 3.5 times relative to normal values. At the same time, the body fought against oxidative stress by increasing the activity of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase. All patients with GC showed pronounced oxidative stress in the blood plasma in the form of a 45-fold increase in malondialdehyde. The activity of the main antioxidant enzyme superoxide dismutase was reduced in GC. Catalase was activated, which indicated pronounced oxidative stress, significant damage to blood vessels, and massive cell death. Glutathione-related enzymes (glutathione S-transferase and glutathione peroxidase) and the antioxidant protein ceruloplasmin were activated, which also indicated significant oxidative stress and severe intoxication in patients with GC.Conclusion. Depending on the stage and type of cancer, an in-depth study of lipid peroxidation and factors of the antioxidant defense system can be used to correct therapy and prevent cancer and can serve as markers of progression and prognosis in gastric cancer. 

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