Evaluating the Effectiveness of Iranian and Korean Injectable Intracanal Calcium Hydroxide on Candida albicans, In vitro

The purpose of this study was to investigate the influence of serum and necrotic soft tissue on the antimicrobial activity of intracanal medicaments. The medicaments tested were: calcium hydroxyde/glycerin paste, calcium hydroxide/chlorhexidine paste, calcium hydroxide/camphorated paramonochlorophenol/glycerin paste, and chlorhexidine/zinc oxide paste. Survival of Enterococcus faecalis and Candida albicans exposed to the medicaments tested in the presence or absence of serum or necrotic tissue was monitored in three in vitro experiments where samples for culturing were taken at different time periods. The overall results demonstrated that the antimicrobial activity of all intracanal medicaments tested was slowed down in the presence of necrotic tissue. Calcium hydroxide pastes in glycerin or chlorhexidine were significantly affected by serum. Of the medicaments tested in this study, the least affected was the calcium hydroxide/camphorated paramonochlorophenol/glycerin paste.

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In vitro susceptibility of oral Candida albicans strains to different pH levels and calcium hydroxide saturated aqueous solution

Brazilian Dental Journal ◽

10.1590/s0103-64402012000300002 ◽

2012 ◽

Vol 23 (3) ◽

pp. 192-198 ◽

Cited By ~ 3

Author(s):

Paulo Henrique Weckwerth ◽

Cristiane Carnietto ◽

Ana Carolina Villas Boas Weckwerth ◽

Marco Antonio Hungaro Duarte ◽

Milton Carlos Kuga ◽

...

Keyword(s):

Aqueous Solution ◽

Candida Albicans ◽

Oral Cavity ◽

Calcium Hydroxide ◽

Culture Broth ◽

Saturated Aqueous Solution ◽

Chi Square ◽

Agar Culture ◽

In Vitro Susceptibility

Candida albicans is present in the oral cavity and in the whole digestive tract of humans and other animals, being frequently related to endodontic treatment failure. The present study determined the incidence of C. albicans in the oral cavity and the susceptibility of isolates to different pH values and saturated calcium hydroxide aqueous solution at pH 12.5. Sixty-five patients attending the Endodontic Clinic at the Sagrado Coração University participated in the study. The collected samples were cultivated in selective media for C. albicans and the isolates were tested in terms of resistance to both alkaline pH and saturated aqueous solution of calcium hydroxide. In relation to time variables, yeast viability was assessed by the Sabouraud's agar culture and fluorescein diacetate and ethidium bromide fluorescent staining method. Results from the different pHs and experimental times, including those from different techniques measuring fungal viability, were compared using the chi-square and Fisher's exact tests (α=0.05). The yeasts became completely inviable after 48 h of contact with the calcium hydroxide solution. On the other hand, when exposed to the alkaline culture broth, the yeasts were found to be viable at pHs 9.5 and 10.5 for up to 7 days. In conclusion, C. albicans can only be completely inhibited by direct contact with saturated calcium hydroxide aqueous solution after 48 h of exposure.

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In vitro assessment of the effectiveness of chlorhexidine gel and calcium hydroxide paste with chlorhexidine against Enterococcus faecalis and Candida albicans

Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology ◽

10.1016/j.tripleo.2006.02.022 ◽

2006 ◽

Vol 102 (2) ◽

pp. e27-e31 ◽

Cited By ~ 43

Author(s):

Ertuğrul Ercan ◽

Mehmet Dalli ◽

Ç.Türksel Dülgergil

Keyword(s):

Candida Albicans ◽

Calcium Hydroxide ◽

Enterococcus Faecalis ◽

In Vitro Assessment ◽

Chlorhexidine Gel

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Antifungal Efficacy of Triple Antibiotic Paste, Double Antibiotic Paste with Fungicide and Calcium Hydroxide with Chitosan as a Vehicle against Candida albicans: An In vitro Study

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i45a32757 ◽

2021 ◽

pp. 394-401

Author(s):

Seera Sudhakar Naidu ◽

Vemareddy Rajashekar ◽

Korrai Balaraju ◽

Chava Gayathri ◽

Garapati Harshitha ◽

...

Keyword(s):

Candida Albicans ◽

Calcium Hydroxide ◽

Antimicrobial Agents ◽

Drug Carrier ◽

Antifungal Drugs ◽

Local Drug Delivery ◽

Antibiotic Powder ◽

Inhibition Zones ◽

Double Antibiotic Paste

Introduction: The purpose of current research is to assess the in vitro antimicrobial efficacy of different medicaments with two different vehicles against Candida albicans (C. albicans). Materials and Methodology: An agar well diffusion assay was used to determine the experimental medicaments' efficacy against C. albicans. Medicaments were divided into six groups, which includes Triple antibiotic powder (TAP) with saline or chitosan, Double antibiotic powder with fungicide (DAPF) with saline or Chitosan, and calcium hydroxide with saline or Chitosan. The diameters of growth inhibition zones were recorded and compared for each group for three days, i.e., 1,4,7 days. The differences between groups were analyzed by Kruskal-Wallis and Mann-Whitney U tests. Results: The largest inhibition zones were observed for the double antibiotic paste + fungicide with chitosan and the smallest for Ca(OH)2 with saline. Over a week, zones of inhibition were consistent only with group V – double antibiotic paste with fungicide and chitosan as the vehicle. Conclusion: DAPF with chitosan is more efficient against Candida albicans. Since the endodontic infections are polymicrobial in origin, the combined local drug delivery of antimicrobial agents such as ciprofloxacin, metronidazole, doxycycline along with antifungal drugs such as fluconazole with an efficient drug carrier (chitosan) is recommended to combat the microbial load.

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AN IN VITRO COMPARISON OF ANTIMICROBIAL EFFICACY OF TRIPLE ANTIBIOTIC PASTE, CALCIUM HYDROXIDE AND ACACIA NILOTICA (BABBULA) AGAINST ENTEROCOCCUS FAECALIS, STREPTOCOCCUS MUTANS AND CANDIDA ALBICANS AS INTRACANAL MEDICAMENTS.

10.36106/gjra/5010113 ◽

2021 ◽

pp. 1-4

Author(s):

Sumarlangki Sayoo ◽

Shubhashini N. ◽

Geeta I. B. ◽

Bindu S

Keyword(s):

Candida Albicans ◽

Calcium Hydroxide ◽

Streptococcus Mutans ◽

Enterococcus Faecalis ◽

Acacia Nilotica ◽

Antimicrobial Efficacy ◽

Diffusion Method ◽

Zone Of Inhibition ◽

Triple Antibiotic Paste

Aim: The aim of this study was to compare the antimicrobial efficacy of Triple antibiotic paste (TAP), Calcium hydroxide and Acacia nilotica (A. nilotica) (Babbula) against Enterococcus faecalis (E. faecalis), Streptococcus mutans (S. mutans) and Candida albicans (C. albicans) as intracanal medicaments. Materials and methods: The triple antibiotic paste mixture of (metronidazole, ciprofloxacin and minocycline), calcium hydroxide, Acacia nilotica (Babbula) were weighed and mixed with polyethylene glycol and agar well diffusion method was employed to study the antimicrobial efficiency against Enterococcus faecalis, Streptococcus mutans and Candida albicans Results: All the intracanal medicaments showed zone of inhibition against Enterococcus faecalis, Streptococcus mutans and Candida albicans. Among all intracanal medicaments used in the study Triple antibiotic paste produced maximum zone of inhibition followed by Babbula and least by Calcium hydroxide. Conclusions: Triple antibiotic paste showed the best antimicrobial efficacy followed by Babbula and least by Calcium hydroxide against Enterococcus faecalis, Streptococcus mutans and Candida albicans. More phytochemical studies need to be done. Further evaluation of concentrated extracts of Acacia nilotica (Babbula) could yield better results.

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