Investigation of Healing Effects of Cinnamic Acid in a Full-Thickness Wound Model in Rabbit

Background: Wounds represent a major global problem for health care systems, clinicians, patients, and their families. Cinnamic Acid (CA) is a naturally occurring phenolic compound that possesses anti-inflammatory, antimicrobial, and antioxidant properties. Objectives: This study aimed to investigate the effects of CA on skin wound-healing in the animal model. Methods: Full-thickness wounds were created on the back of white New Zealand rabbits of both sexes. Animals were divided into six groups (six animals and 12 wounds in each group). Negative control received no treatment, while positive control was treated with phenytoin cream, vehicle group with eucerin, and test groups with 0.1, 1, and 10% CA ointments. The healing activity of CA was evaluated by determining the wound closure rate and hydroxyproline content of wound tissue samples. In addition, the histopathological study of tissue samples of different groups was performed using hematoxylin and eosin staining. Results: The rate of wound closure and hydroxyproline levels of tissue samples in animals treated with CA 0.1% were significantly (P < 0.05) higher than those of no-treatment and vehicle-treated groups. Histological study revealed the increased number of fibroblasts and hair follicles, increased reepithelialization rate, and enhanced neovascularization in CA 0.1%-treated group when compared to no-treatment and vehicle groups. Conclusions: Cinnamic acid at low concentrations (< 1%) is potent for skin wound-healing and could be used as a safe and effective topical healing agent. Further studies are needed to confirm our findings.

Download Full-text

Sericin hydrogels promote skin wound healing with effective regeneration of hair follicles and sebaceous glands after complete loss of epidermis and dermis

Biomaterials Science ◽

10.1039/c8bm00934a ◽

2018 ◽

Vol 6 (11) ◽

pp. 2859-2870 ◽

Cited By ~ 24

Author(s):

Chao Qi ◽

Luming Xu ◽

Yan Deng ◽

Guobin Wang ◽

Zheng Wang ◽

...

Keyword(s):

Wound Healing ◽

Skin Wound ◽

Hair Follicles ◽

Complete Loss ◽

Full Thickness ◽

Sebaceous Glands ◽

Skin Injury ◽

Skin Wound Healing ◽

Full Thickness Skin ◽

Thickness Skin

Treating full-thickness skin injury with photo-crosslinkable sericin hydrogel for scarless regeneration with effective restoration of skin appendages.

Download Full-text

Overexpression of the Oral Mucosa-Specific microRNA-31 Promotes Skin Wound Closure

International Journal of Molecular Sciences ◽

10.3390/ijms20153679 ◽

2019 ◽

Vol 20 (15) ◽

pp. 3679 ◽

Cited By ~ 3

Author(s):

Lin Chen ◽

Alyne Simões ◽

Zujian Chen ◽

Yan Zhao ◽

Xinming Wu ◽

...

Keyword(s):

Wound Healing ◽

Oral Mucosa ◽

Wound Closure ◽

Expression Patterns ◽

Skin Wound ◽

Skin Wound Healing ◽

Specific Expression ◽

Keratinocyte Proliferation

Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of oral mucosa-specific microRNAs. A panel of 57 differentially expressed high expresser microRNAs were identified based on our previously published miR-seq dataset of paired skin and oral mucosal wound-healing [Sci. Rep. (2019) 9:7160]. These microRNAs were further grouped into 5 clusters based on their expression patterns, and their differential expression was confirmed by TaqMan-based quantification of LCM-captured epithelial cells from the wound edges. Of these 5 clusters, Cluster IV (consisting of 8 microRNAs, including miR-31) is most intriguing due to its tissue-specific expression pattern and temporal changes during wound-healing. The in vitro functional assays show that ectopic transfection of miR-31 consistently enhanced keratinocyte proliferation and migration. In vivo, miR-31 mimic treatment led to a statistically significant acceleration of wound closure. Our results demonstrate that wound-healing can be enhanced in skin through the overexpression of microRNAs that are highly expressed in the privileged healing response of the oral mucosa.

Download Full-text

Keratin Scaffolds Containing Casomorphin Stimulate Macrophage Infiltration and Accelerate Full-Thickness Cutaneous Wound Healing in Diabetic Mice

Molecules ◽

10.3390/molecules26092554 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2554

Author(s):

Marek Konop ◽

Anna K. Laskowska ◽

Mateusz Rybka ◽

Ewa Kłodzińska ◽

Dorota Sulejczak ◽

...

Keyword(s):

Wound Healing ◽

Wound Dressing ◽

Healing Process ◽

Skin Wound ◽

Wound Healing Process ◽

Diabetic Mice ◽

Full Thickness ◽

Skin Wound Healing ◽

Impaired Wound Healing

Impaired wound healing is a major medical challenge, especially in diabetics. Over the centuries, the main goal of tissue engineering and regenerative medicine has been to invent biomaterials that accelerate the wound healing process. In this context, keratin-derived biomaterial is a promising candidate due to its biocompatibility and biodegradability. In this study, we evaluated an insoluble fraction of keratin containing casomorphin as a wound dressing in a full-thickness surgical skin wound model in mice (n = 20) with iatrogenically induced diabetes. Casomorphin, an opioid peptide with analgesic properties, was incorporated into keratin and shown to be slowly released from the dressing. An in vitro study showed that keratin-casomorphin dressing is biocompatible, non-toxic, and supports cell growth. In vivo experiments demonstrated that keratin-casomorphin dressing significantly (p < 0.05) accelerates the whole process of skin wound healing to the its final stage. Wounds covered with keratin-casomorphin dressing underwent reepithelization faster, ending up with a thicker epidermis than control wounds, as confirmed by histopathological and immunohistochemical examinations. This investigated dressing stimulated macrophages infiltration, which favors tissue remodeling and regeneration, unlike in the control wounds in which neutrophils predominated. Additionally, in dressed wounds, the number of microhemorrhages was significantly decreased (p < 0.05) as compared with control wounds. The dressing was naturally incorporated into regenerating tissue during the wound healing process. Applied keratin dressing favored reconstruction of more regular skin structure and assured better cosmetic outcome in terms of scar formation and appearance. Our results have shown that insoluble keratin wound dressing containing casomorphin supports skin wound healing in diabetic mice.

Download Full-text

The effect of Korean Red Ginseng on full-thickness skin wound healing in rats

Journal of Ginseng Research ◽

10.1016/j.jgr.2017.12.006 ◽

2019 ◽

Vol 43 (2) ◽

pp. 226-235 ◽

Cited By ~ 6

Author(s):

Ki-Soo Park ◽

Dae-Hwan Park

Keyword(s):

Wound Healing ◽

Skin Wound ◽

Full Thickness ◽

Red Ginseng ◽

Korean Red Ginseng ◽

Skin Wound Healing ◽

Full Thickness Skin ◽

Thickness Skin

Download Full-text

Pharmaceutical application of frog skin on full-thickness skin wound healing in mice

Pharmaceutical Biology ◽

10.3109/13880209.2013.804846 ◽

2013 ◽

Vol 51 (12) ◽

pp. 1600-1606 ◽

Cited By ~ 9

Author(s):

Mahere Rezazade Bazaz ◽

Mohammad Mashreghi ◽

Nasser Mahdavi Shahri ◽

Mansour Mashreghi ◽

Ahmad Asoodeh ◽

...

Keyword(s):

Wound Healing ◽

Frog Skin ◽

Skin Wound ◽

Full Thickness ◽

Skin Wound Healing ◽

Full Thickness Skin ◽

Pharmaceutical Application ◽

Thickness Skin

Download Full-text

Carbonic Anhydrase VI in Skin Wound Healing Study on Car6 Knockout Mice

International Journal of Molecular Sciences ◽

10.3390/ijms21145092 ◽

2020 ◽

Vol 21 (14) ◽

pp. 5092

Author(s):

Toini Pemmari ◽

Jaakko Laakso ◽

Maarit S. Patrikainen ◽

Seppo Parkkila ◽

Tero A. H. Järvinen

Keyword(s):

Wound Healing ◽

Knockout Mice ◽

Wound Closure ◽

Skin Wound ◽

Carbonic Anhydrases ◽

Skin Wound Healing ◽

Tumor Cell Migration ◽

Treatment Groups ◽

Thickness Skin ◽

Carbonic Anhydrase Vi

Carbonic anhydrases (CAs) contribute to tumor cell migration by generating an acidic environment through the conversion of carbon dioxide to bicarbonate and a proton. CA VI is secreted to milk and saliva, and it could contribute to wound closure, as a potential trophic factor, in animals that typically lick their wounds. Our aim was to investigate whether human CA VI improves skin-wound healing in full-thickness skin-wound models. The effect was studied in Car6 −/− knockout mice and wild type littermates. Half of both mice strains were given topically administered, milk-derived CA VI after wounding and eight hours later. The amount of topically given CA VI exceeded the predicted amount of natural saliva-delivered CA VI. The healing was followed for seven days and studied from photographs and histological sections. Our results showed no significant differences between the treatment groups in wound closure, re-epithelization, or granulation tissue formation, nor did the Car6 genotype affect the healing. Our results demonstrate that CA VI does not play a major role in skin-wound healing and also suggest that saliva-derived CA VI is not responsible for the licking-associated improved wound healing in animals.

Download Full-text

Green fabrication of seedbed-like Flammulina velutipes polysaccharides–derived scaffolds accelerating full-thickness skin wound healing accompanied by hair follicle regeneration

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2020.11.154 ◽

2021 ◽

Vol 167 ◽

pp. 117-129

Author(s):

Feixiang Chen ◽

Qiang Zhang ◽

Ping Wu ◽

Yanan Zhao ◽

Xiaotong Suo ◽

...

Keyword(s):

Wound Healing ◽

Hair Follicle ◽

Skin Wound ◽

Flammulina Velutipes ◽

Full Thickness ◽

Skin Wound Healing ◽

Full Thickness Skin ◽

Thickness Skin

Download Full-text

Effect of Combining Low Temperature Plasma, Negative Pressure Wound Therapy, and Bone Marrow Mesenchymal Stem Cells on an Acute Skin Wound Healing Mouse Model

International Journal of Molecular Sciences ◽

10.3390/ijms21103675 ◽

2020 ◽

Vol 21 (10) ◽

pp. 3675 ◽

Cited By ~ 1

Author(s):

Hui Song Cui ◽

So Young Joo ◽

Yoon Soo Cho ◽

Ji Heon Park ◽

June-Bum Kim ◽

...

Keyword(s):

Wound Healing ◽

Combination Therapy ◽

Wound Closure ◽

Negative Pressure Wound Therapy ◽

Skin Wound ◽

Low Temperature Plasma ◽

Wound Therapy ◽

Skin Wound Healing ◽

Temperature Plasma ◽

Marrow Mesenchymal Stem Cells

Low-temperature plasma (LTP; 3 min/day), negative pressure wound therapy (NPWT; 4 h/day), and bone marrow mesenchymal stem cells (MSCs; 1 × 106 cells/day) were used as mono- and combination therapy in an acute excisional skin wound-healing ICR mouse model. These therapies have been beneficial in treating wounds. We investigated the effectiveness of monotherapy with LTP, NPWT, and MSC and combination therapy with LTP + MSC, LTP + NPWT, NPWT + MSC, and LTP + NPWT + MSC on skin wounds in mice for seven consecutive days. Gene expression, protein expression, and epithelial thickness were analyzed using real time polymerase chain reaction (RT-qPCR), western blotting, and hematoxylin and eosin staining (H&E), respectively. Wound closure was also evaluated. Wound closure was significantly accelerated in monotherapy groups, whereas more accelerated in combination therapy groups. Tumor necrosis factor-α (TNF-α) expression was increased in the LTP monotherapy group but decreased in the NPWT, MSC, and combination therapy groups. Expressions of vascular endothelial growth factor (VEGF), α-smooth muscle actin (α-SMA), and type I collagen were increased in the combination therapy groups. Re-epithelialization was also considerably accelerated in combination therapy groups. Our findings suggest that combination therapy with LPT, NPWT, and MSC exert a synergistic effect on wound healing, representing a promising strategy for the treatment of acute wounds.

Download Full-text