Pseudopapillary Solid Cystic Tumor Arising From an Extrapancreatic Site

2001 ◽  
Vol 125 (10) ◽  
pp. 1368-1371
Author(s):  
Masaharu Fukunaga
Keyword(s):  
Flow Cytometry ◽  
Tumor Cells ◽  
Epithelial Membrane Antigen ◽  
Pancreatic Tissue ◽  
S Phase ◽  
Growth Patterns ◽  
Cystic Tumor ◽  
Dense Granules ◽  
Eosinophilic Cytoplasm ◽  
Solid Cystic Tumor

Abstract A case of pseudopapillary solid cystic tumor arising in the omentum of a 46-year-old woman is presented. A well-defined, encapsulated tumor measuring 5.2 × 4.0 × 4.0 cm was histologically characterized by a combination of solid and pseudopapillary growth patterns of tumor cells with abundant pale-to-eosinophilic cytoplasm. No pancreatic tissue was observed within or adjacent to the tumor. Immunohistochemically, the tumor was positive for vimentin, epithelial membrane antigen, and α1-antichymotrypsin. Ultrastructurally, the tumor cells contained electron dense granules of variable sizes, most likely representing lysosomes. Flow cytometry showed a diploid DNA content with a high S-phase fraction. The patient was well without recurrence 3 months after diagnosis. It is important to include pseudopapillary solid cystic tumor in the differential diagnosis of omental tumors.

scholarly journals Proportion of S-phase tumor cells measured by flow cytometry is an independent prognostic factor in meningioma tumors

Cytometry ◽  
1999 ◽  
Vol 38 (3) ◽  
pp. 118-123 ◽  
Author(s):  
A. Ma�llo ◽  
P. D�az ◽  
A. Blanco ◽  
A. L�pez ◽  
J. Ciudad ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Prognostic Factor ◽  
Tumor Cells ◽  
S Phase ◽  
Independent Prognostic Factor

scholarly journals Ovarian female adnexal tumor of probable Wolffian origin – Case report

Open Medicine ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. 899-903
Author(s):  
Ljiljana Vučković ◽  
Aleksandra Klisic ◽  
Mirjana Miladinović
Keyword(s):  
Case Report ◽  
Tumor Cells ◽  
Epithelial Membrane Antigen ◽  
Neuron Specific Enolase ◽  
Diagnostic Algorithm ◽  
Accurate Diagnosis ◽  
Histopathological Analysis ◽  
Eosinophilic Cytoplasm ◽  
Adnexal Tumor ◽  
Adnexal Tumors

Abstract Background During embryonic development in women, a regression of temporary embryonic structures – mesonephric (Wolffian) ducts occurs. Adnexal tumors of Wolffian duct origin (FATWO) are rare. Case report We presented the case of a 64-year-old female patient who was diagnosed with FATWO. After the surgical treatment, the uterus with bilateral adnexal structures was submitted for histopathological analysis. The left ovary was occupied by a tumor measuring 80 × 60 × 50 mm, with smooth, shiny, whitish surface. Tumor cells were medium-sized, relatively uniform, round, and polygonal, with eosinophilic cytoplasm and centrally laid nucleus with fine chromatin, organized into solid, trabecular, and tubular formations. Tumor cells were positive for pancytokeratin (CK), CK7, CD10, neuron-specific enolase (NSE), synaptophysin, calretinin, progesterone, estrogen, and epithelial membrane antigen (EMA). Conclusion This case adds a report of a rare tumor to the literature. We must think of it in the differential diagnostic algorithm to make an accurate diagnosis for selecting the best treatment modality.

scholarly journals Extragastrointestinal stromal tumor of the abdominal subcutaneous tissue: Report of a very rare case at an unusual site

2017 ◽  
Vol 45 (3) ◽  
pp. 1273-1278 ◽  
Author(s):  
Xue He ◽  
Nannan Chen ◽  
Li Lin ◽  
Congyang Wang ◽  
Yan Wang
Keyword(s):  
Tumor Cells ◽  
Subcutaneous Tissue ◽  
Dermatofibrosarcoma Protuberans ◽  
Pathological Examination ◽  
Chinese Han ◽  
Unusual Site ◽  
Stromal Tumors ◽  
Activating Mutations ◽  
Eosinophilic Cytoplasm ◽  
Exon 11

Extragastrointestinal stromal tumors (EGISTs) are rare tumors that arise outside the digestive tract. We report a case of an EGIST arising in the subcutaneous tissue of the abdominal wall, which at this site can often be misdiagnosed as dermatofibrosarcoma protuberans. The tumor was surgically resected from a 72-year-old male Chinese Han patient, and pathological examination revealed spindle-shaped tumor cells with eosinophilic cytoplasm and an oval nucleus. Immunohistochemically, the tumor cells showed strong cytoplasmic positivity for CD34, c-KIT (CD117), and DOG1. Tests for activating mutations of GISTs showed that the tumor cells carried an in-frame deletion (NP_000213.1:p.Lys550_Gln556del) in exon 11 of c-KIT (CD117). Thus, an EGIST should be considered in patients with abdominal subcutaneous tumors with an epithelioid, spindle-shaped, or mixed morphology. Immunohistochemistry of c-KIT (CD117) and DOG1 and genetic testing for activating mutations are recommended to aid in the differential diagnosis of subcutaneous tumors. In short, although EGISTs are rare in the abdominal subcutaneous tissue, pathologists must be aware of their possibility.

scholarly journals Postoperative metastasis prediction based on portal vein circulating tumor cells detected by flow cytometry in periampullary or pancreatic cancer

2019 ◽  
Vol Volume 11 ◽  
pp. 7405-7425 ◽  
Author(s):  
Lianyuan Tao ◽  
Li Su ◽  
Chunhui Yuan ◽  
Zhaolai Ma ◽  
Lingfu Zhang ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Flow Cytometry ◽  
Portal Vein ◽  
Tumor Cells ◽  
Circulating Tumor Cells

Mitomycin Induced Apoptosis in Human Leukemia K562 Cells

2012 ◽  
Vol 599 ◽  
pp. 71-75
Author(s):  
Shu Li Shao ◽  
Bin Zhao ◽  
Wei Wei Zhang ◽  
Wei Zhao ◽  
Guang Hui Wu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Gel Electrophoresis ◽  
Light Microscope ◽  
Previous Experiment ◽  
K562 Cells ◽  
S Phase ◽  
Fluorescence Microscope ◽  
Human Leukemia ◽  
Induced Apoptosis ◽  
Human Leukemia K562 Cells

Objective: The research aimed to study the effects of mitomycin on human leukemic K562 cells, and to explore the mechanism of mitomycin induced apoptosis．In order to provide previous experiment basis for mitomycin applying clinical treatments Methods: The multiplication and apoptosis status of K562 cells treated different time by different concentration mitomycin were observed by light microscope, fluorescence microscope, TEM, agrose gel electrophoresis of DNA and flow cytometry. Results: The results showed that mitomycin could induce K562 cells apoptosis, and the best concentration was 12.5μg/ml for 48 h. The optimal concentration of apoptosis induced by apoptosis rate is （28.8±1.04）% （P<0.01）. Mitomycin could affect the S phase among cellular multiplication, cell could be blocked by mitomycin and then apoptosis in this phase. Conclusions: Mitomycin can induce the apoptosis of human leukemic K562 cells. It is of great significance to guide clinical medication.

scholarly journals DNA and S-phase fraction analysis by flow cytometry in prostate cancer. Clinicopathologic implications

Cancer ◽  
1993 ◽  
Vol 71 (4) ◽  
pp. 1289-1296 ◽  
Author(s):  
Nicola Tinari ◽  
Clara Natoli ◽  
Domenico Angelucci ◽  
Raffaele Tenaglia ◽  
Bruno Fiorentino ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Flow Cytometry ◽  
S Phase ◽  
Phase Fraction

scholarly journals In vivo quantitation of rare circulating tumor cells by multiphoton intravital flow cytometry

2007 ◽  
Vol 104 (28) ◽  
pp. 11760-11765 ◽  
Author(s):  
W. He ◽  
H. Wang ◽  
L. C. Hartmann ◽  
J.-X. Cheng ◽  
P. S. Low
Keyword(s):  
Flow Cytometry ◽  
Tumor Cells ◽  
Circulating Tumor Cells

scholarly journals Determination of Circulating Tumor Cells by Flow Cytometry in the Bladder Cancer Patients

2018 ◽  
Vol 2 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Elif Ercan ◽  
◽  
Ender Sımsek ◽  
Ozen Ozensoy Guler ◽  
Abdullah Erdem Canda ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Bladder Cancer ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Circulating Tumor Cells

Regulation of the start of DNA replication in Schizosaccharomyces pombe

1999 ◽  
Vol 112 (6) ◽  
pp. 939-946 ◽  
Author(s):  
C.R. Carlson ◽  
B. Grallert ◽  
T. Stokke ◽  
E. Boye
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Growth Rate ◽  
Schizosaccharomyces Pombe ◽  
Growth Rates ◽  
Cell Separation ◽  
Cell Mass ◽  
Nitrogen Sources ◽  
S Phase ◽  
G1 Phase

Cells of Schizosaccharomyces pombe were grown in minimal medium with different nitrogen sources under steady-state conditions, with doubling times ranging from 2.5 to 14 hours. Flow cytometry and fluorescence microscopy confirmed earlier findings that at rapid growth rates, the G1 phase was short and cell separation occurred at the end of S phase. For some nitrogen sources, the growth rate was greatly decreased, the G1 phase occupied 30–50% of the cell cycle, and cell separation occurred in early G1. In contrast, other nitrogen sources supported low growth rates without any significant increase in G1 duration. The method described allows manipulation of the length of G1 and the relative cell cycle position of S phase in wild-type cells. Cell mass was measured by flow cytometry as scattered light and as protein-associated fluorescence. The extensions of G1 were not related to cell mass at entry into S phase. Our data do not support the hypothesis that the cells must reach a certain fixed, critical mass before entry into S. We suggest that cell mass at the G1/S transition point is variable and determined by a set of molecular parameters. In the present experiments, these parameters were influenced by the different nitrogen sources in a way that was independent of the actual growth rate.

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