scholarly journals Dormancy and Germination In Vitro Response of Hydrangea macrophylla and Hydrangea paniculata Seed to Light, Cold-Treatment and Gibberellic Acid

2010 ◽  
Vol 28 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Stephen P. Greer ◽  
Timothy A. Rinehart
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Red Light ◽  
Cold Treatment ◽  
Seed Viability ◽  
In Vitro Study ◽  
Potassium Nitrate ◽  
Hydrangea Macrophylla ◽  
Solid Media

Abstract Seed germination was optimized for ten Hydrangea macrophylla (Thunb.) Ser. and two Hydrangea paniculata Siebold cultivars in vitro. Methods were also developed to assay seed physiology. Best results for in vitro study were obtained with 0.5× Gamborgs solid media in conjunction with Plant Preservative Mixture (PPM), and by sterilizing seed with trichloro-s-triazinetrione (Trichlor). Assays of physiology were conducted by sterilizing seed and treating with combinations of white and red light, cold-treatment, gibberellic acid and potassium nitrate, and light cycles. Estimates of seed viability/dormancy, germination of non-dormant seed, and germination overall were calculated for each treatment combination. The most favorable conditions for overall Hydrangea seed germination were cold-treatment for 6 weeks, imbibition with GA3 + KNO3, and plating on half-strength Gamborgs media supplemented with GA3 in the presence of white light.

scholarly journals Methods of breaking seed dormancy for ornamental passion fruit species

2017 ◽  
Vol 23 (1) ◽  
pp. 72 ◽  
Author(s):  
Thalita Neves Marostega ◽  
Petterson Baptista Da Luz ◽  
Armando Reis Tavares ◽  
Leonarda Grillo Neves ◽  
Severino De Paiva Sobrinho
Keyword(s):  
Experimental Design ◽  
Seed Germination ◽  
Gibberellic Acid ◽  
Seed Dormancy ◽  
Potassium Nitrate ◽  
Plant Production ◽  
Commercial Plant ◽  
Fruit Species ◽  
Breaking Seed Dormancy ◽  
And Control

The Passiflora L. genus covers a diversity of wild species with ornamental potential, especially due to the intrinsic beauty of its exotic flowers, flowering more than once a year and the lush foliage. However, Passiflora seeds present dormancy complicating seed germination and the establishment of commercial plant production with species with high ornamental potential. This study was conducted to determine the best pre-germination treatments to overcome seed dormancy for Passiflora quadrangularis, P. nitida, P. foetida, P. eichleriana, P. alata, P. cincinnata, P. mucronata, P. micropetala, P. suberosa, P. morifolia and P. tenuifila. The experimental design was completely randomized, with five treatments and four replicates, with 25 seeds per plot. Pre-germination treatments were: seeds soaked in 1,000 mg L- 1 GA3 (gibberellic acid) for 6 hours, seeds soaked in 0.2 % KNO3 (potassium nitrate) for 24 hours, seeds soaked in 1 % KNO3 for 24 hours, partial seedcoat scarification with sandpaper number 120 and control (seeds untreated). Percentage of germination, germination velocity index and radicle length were evaluated for all species. The results showed that GA3 was effective to overcome seed dormancy in P. suberosa (86%), P. morifolia (68 %) and P. tenuifila (54%). KNO3 1% had significant effect on overcoming dormancy in seeds of P. eichleriana (66%) and scarification with sandpaper increased seed germination of P. micropetala (38%).

scholarly journals The Effect of Sodium Hypochlorite (NaOCl) and Heat Treatments on Seed Germination of Rice: An Approach to Restore Seed Viability

2021 ◽  
pp. 49-53
Author(s):  
Monoj Sutradhar ◽  
Subhasis Samanta ◽  
Brijesh Kumar Singh ◽  
Md. Nasim Ali ◽  
Nirmal Mandal
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Sodium Hypochlorite ◽  
Seed Viability ◽  
Germination Percentage ◽  
Heat Treated ◽  
Mother Plants ◽  
Breaking Seed Dormancy ◽  
One Year

Dormancy in rice serves as a mechanism of survival by protecting the seed from germinating in the mother plants; however, it becomes a problem in germination during sowing in soil or under in vitro conditions. This study was conducted to determine the effect of heat treatment and sodium hypochlorite (NaOCl) treatment of seeds on dormancy alleviation. The seeds included both freshly harvested seeds and one-year-old stored seeds, which were tested for germination after different types of seed treatments. Both the treatments increased the germination percentage in seeds, however, it was lesser in the case of old seeds. The best results were obtained from 2% NaOCl treatment for 24 hrs in new seeds, i.e. 92.84±0.103 % germination percentage (GP). However, the higher GP in old seeds were obtained from 48 hrs of heat-treated seeds i.e. 82.9±0.509 % GP. The results of the experiment revealed that rice seeds start to lose viability within a year due to seed dormancy, but this can be reversed with proper measures. These methods of breaking seed dormancy can be considered effective to break seed dormancy and improve seed germination in rice.

scholarly journals Efeito da aplicação prévia ou adição de dessensibilizantes em géis de peróxido de hidrogênio no esmalte clareado

2021 ◽  
Vol 10 (7) ◽  
pp. e18610716467
Author(s):  
Vinicius Matheus Amorim Macêdo ◽  
Mariana Evangelista Santos ◽  
Rosângela Marques Duarte ◽  
Sônia Saeger Meireles
Keyword(s):  
Vickers Microhardness ◽  
In Vitro Study ◽  
Potassium Nitrate ◽  
The Other ◽  
Color Measurement ◽  
Whitening Effect ◽  
Treatment Groups ◽  
Desensitizing Agents ◽  
Bleaching Gels

This in vitro study evaluated the effect of 35% hydrogen peroxide (35HP) gels with different desensitizing agents on color, microhardness and roughness of bleached enamel. Forty enamel-dentin specimens (6x6x2 mm2) were obtained from twenty human molars. After color measurement with a spectrophotometer, the specimens were randomized into four groups (n= 10): 35HPw- 35HP without desensitizing agent; 35HPCa- 35HP with calcium; 35HPK- 35HP with 0.5% potassium nitrate; 35HPTFa- 35HPw + topical application of 5% potassium nitrate and 2% sodium fluoride (TFa). Specimens were evaluated for color (∆Eab* and ∆E00), Vickers microhardness (VHN) and, superficial and volumetric roughness (Ra and Sa, µm) using a 3D non-contact profilometer before and 1-week after bleaching. Data were analyzed by ANOVA and Tukey tests (p< 0.05). Both treatment groups promoted a significant whitening effect and there was no difference between them for any color parameters evaluated. All groups reduced significantly the VHN, but the 35HPK showed a reduction significantly major than the other groups. 35HPCa and 35HP+TFa did not avoid the of Ra and Sa increasing. It can be concluded that 35HP bleaching gels with different desensitizing agents did not affect the whitening efficacy. However, all treatments decreased the microhardness, the addition of calcium into 35HP gel and the application of TFa before bleaching did not revert the enamel roughness under in vitro conditions.

In vitro Organogenesis in Cotton (Gossypium spp) for Ex-situ Conservation Issue

2021 ◽  
pp. 108-119
Author(s):  
Marius Guillaume Sinha ◽  
Gilles Habib Todjro Cacaï ◽  
Jerome Anani Houngue ◽  
Serge Sètondji Houédjissin ◽  
Thiéris Dassoudo Sossou ◽  
...  
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Activated Charcoal ◽  
Genetic Erosion ◽  
Ex Situ ◽  
Naphthalene Acetic Acid ◽  
Zygotic Embryos ◽  
Local Varieties ◽  
Regeneration Rate

Background: Monovarietal cultivation of cotton plant allowed the genetic erosion for traditional cotton varieties that proceed essential traits mainly involved in cotton genetic breeding. These varieties need to be preserved for future used. This study aims to evaluate the effect of gibberellic acid on cotton seed germination and the effect of Benzylaminopurin (BAP), Kinetin (KIN), α-naphthalene acetic acid (NAA) and activated charcoal (CA) on cotton seedlings growth obtained from different type of explants. Methodology: The seeds of three improved varieties (KET782, ANG956, OKP768) and five local varieties (Q62, Q64, Q85, Q88, Q92) were germinated with different concentration of gibberellic acid (GA3). Different explants were collected from the germinated plantlets and cultivated in different culture media containing plants growth regulators and activated charcoal in different concentration. Results: We found that Gibberellic Acid activity on cotton seeds germination significantly varied according to the variety and GA3 concentration in the medium. 63.33% germination rate in OKP768 was obtained on the medium containing 0.5 mg/L of GA3 while the medium with 1 mg/l of GA3 gave the highest seed germination in Q85 (75%), Q64 (69.17%), and ANG956 (40.83%). The plantlets regeneration rate varied within the explants in different varieties used. 100%of axillary nodes were regenerated in Q62; Q85 and Q92 varieties while 60% were regenerated with the zygotic embryos in ANG956, and OKP768. Moreover, the medium supplemented with GA3 (1mg/l) + NAA (1 mg/l) induced the greatest number of roots (2.75 roots/plantlet) in the ANG956 and OKP768 varieties. With activated charcoal (10 g/l), ANG956 and OKP768 varieties achieved better performance with respective roots length average of 3.4 cm/plantlet and 2.1 cm/plantlet. The activated charcoal at 10 g/l highly influenced the length of roots with an average of 7.7cm in ANG956 variety. Implication: The protocol established during this study will be useful for in vitro regeneration and conservation for cotton local varieties.

scholarly journals SYNCHRONIZING THE in vitro GERMINATION OF Psidium guineense Sw. SEEDS BY MEANS OF OSMOTIC PRIMING

2016 ◽  
Vol 40 (4) ◽  
pp. 649-660 ◽  
Author(s):  
Márcia Adriana Carvalho dos Santos ◽  
Mailson Monteiro do Rego ◽  
Manoel Abilio de Queiroz ◽  
Bárbara França Dantas ◽  
Wagner Campos Otoni
Keyword(s):  
Seed Germination ◽  
Potassium Nitrate ◽  
Germination Percentage ◽  
In Vitro Germination ◽  
Peg 6000 ◽  
Caatinga Biome ◽  
Germination Speed ◽  
Mean Germination Time ◽  
Positive Effect

ABSTRACT The Brazilian guava (Psidium guineense Swartz) is seed-propagated and, being native to the Caatinga biome, may frequently have uneven germination.Thus, we aimed to evaluate the synchronization of the in vitro seed germination of three accessions of the Brazilian guava, using water, polyethyleneglycol (PEG 6000), and potassium nitrate (KNO3) at different potentials and times of osmotic priming. Seeds from three accessions of the Brazilian guava (Y85, Y93,and Y97) from the UNEB/BA Germplasm Active Bank were subjected to the following pretreatments: -0.6, -1.0, -1.4, and -1,8 MPa PEG 6000; 10 and 20% KNO3 for 24h; 10 and 20% KNO3 for 48h; water for 24 and 48h; and non-primed seeds as the control. The experimental design was therefore a 10x3+1 factorial scheme. We assessed the germination percentage (G), mean germination time (MGT), germination speed (GS), and germination speed index (GSI). Data was subjected to analysis of variance followed by a means test (Duncan at 5% probability) and regression. There was interaction between the priming treatments and accessions for all evaluated features, except G. PEG 6000 decreased the MGT (from 6 to 8 days) and increased GS and GSI of seeds from all three accessions at potentials -1.0 to -1.5 MPa.Water-priming had a positive effect on MGT, GS, and GSI of accession Y85 seeds. KNO3 negatively affected germination of seeds from all three accessions. Thereby, we could synchronize seed germination of accessions Y85 and Y97 with PEG 6000.

scholarly journals In Vitro Decoated Seed Germination and Seedling Development for Propagation of Wild Mandrake (Mandragora autumnalis Bertol.)

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1339
Author(s):  
Hani Al-Ahmad
Keyword(s):  
Seed Germination ◽  
Zygotic Embryo ◽  
Seedling Emergence ◽  
Cold Treatment ◽  
Fresh Weight ◽  
Plant Materials ◽  
System P ◽  
Double Number

The establishment of an efficient in vitro propagation system for the conservation of the Mediterranean Mandragora autumnalis is highly desirable due to its scarcity, besides its potential medicinal and pharmacological properties. In a separate unpublished study, this species has proved to be resistant to laboratory plant regeneration from vegetative tissue cultures; therefore, an alternative decoated seed (i.e., endosperm enclosed the zygotic embryo) germination approach was conducted in this study. Pre-cold treatment of M. autumnalis seeds, removal of seed coats, and exogenous application of gibberellic acid (GA3) promoted in vitro seed germination and seedling emergence. In two separate experiments, approximately 10–27% of the germinated decoated seeds developed healthy seedlings within two weeks, compared to the non-germinated intact seeds of the potting soil controls. After 72 days, the highest rates of healthy seedlings development (67.4 and 69.4%) achieved in the in vitro decoated seed cultures supplemented with 60 and 100 mg/L GA3, respectively, compared to only 25% seedlings emergence rate of the in vitro cultures devoid of GA3, and 44.2% of the soil controls. The in vitro developed plants were healthy, survived transplantation conditions, and, significantly, grew faster, formed on average more than the double number of true leaves and shoot fresh weight (p ≤ 0.05), 90% more fresh weight of root system (p ≤ 0.05), and ultimately more than the double gross fresh weight (p ≤ 0.05) than that of the in vivo developed plants of the soil controls. Such in vitro seed germination approaches would be favorable due to the higher capacity of uniform seedling establishment year-round under lab-controlled conditions, facilitating proliferation and conservation of rare and threatened species, and providing fresh and axenic plant materials required for downstream studies such as those associated with leaf-derived protoplasts and genetic transformations.

PHOTOTHERAPY OF GINGIVITIS: PILOT CLINICAL STUDY

2011 ◽  
Vol 04 (04) ◽  
pp. 437-446 ◽  
Author(s):  
ELINA A. GENINA ◽  
VLADIMIR A. TITORENKO ◽  
VALERY V. TUCHIN ◽  
GEORGY V. SIMONENKO ◽  
ALEXEY N. BASHKATOV ◽  
...  
Keyword(s):  
Clinical Study ◽  
Treatment Group ◽  
Red Light ◽  
In Vitro Study ◽  
Control Group ◽  
Mechanical Removal ◽  
Pilot Clinical Study ◽  
Photodynamic Reaction ◽  
Microbial Action

The goal of this work was to evaluate the safety and efficacy of the Red Light Emitted Toothbrush (R-LETB) emitting at wavelength of 663 nm with power density of 3.3 mW/cm2 in combination with 0.1%-methylene blue (MB) solution for the reduction of plaque and treatment of gingivitis. A microbiological in vitro study and a pilot clinical study were conducted. The microbiological study has shown total suppression of pathogenic flora after a 3-min exposure to the dye solution followed by a 20-sec treatment with the R-LETB. For the clinical study, 37 subjects of both sexes with gingivitis were enrolled and randomly assigned to one of two groups. Subjects in the first (treatment) group were instructed to rinse their mouth with MB solution provided for 1 min and then brush the teeth with the R-LETB and standardized toothpaste. The second (control) group used only the toothpaste and a regular toothbrush. Subjects in both groups followed their respective procedures 2 times a day (morning and evening) for 30 days. Indices of plaque, gingival bleeding, and inflammation were evaluated at 14-day and 30-day timepoints. In the both groups, all indices improved in comparison with baseline. However, the treatment group demonstrated more pronounced improvement of the studied indices that was attributed to additional anti-microbial action of red light and MB on gum tissue. Thus, the use of R-LETB with MB appears to have a multifactor therapeutic action on oral pathological microflora: mechanical removal of the bacteria and suppressing action on microorganisms due to photodynamic reaction.

scholarly journals Characteristics of Litchi Seed Germination

HortScience ◽  
1996 ◽  
Vol 31 (7) ◽  
pp. 1187-1189 ◽  
Author(s):  
J.S. Prasad ◽  
Raj Kumar ◽  
Mukund Mishra ◽  
Rajesh Kumar ◽  
A.K. Singh ◽  
...  
Keyword(s):  
Seed Germination ◽  
Relative Humidity ◽  
Gibberellic Acid ◽  
Butyric Acid ◽  
Growth Regulators ◽  
Filter Paper ◽  
Seed Viability ◽  
Indole Butyric Acid ◽  
Polyethylene Bags ◽  
Better Than

Seed germination of four Litchi chinensis Sonn. cultivars (`Deshi', `Kasba', `Purbi', and `Early Bedana') was studied under various conditions, viz. in soil beds exposed to sunlight or in shade, in sand beds exposed to sunlight or in shade, and on moist filter paper. Among all, shaded, humid sand at 35 ± 2C gave the highest germination. Delaying sowing seeds after removal from the fruit significantly reduced germination. Litchi seeds held in polyethylene bags up to 4 days at 37 ± 2C at 90% relative humidity delayed loss of seed viability. Germination was improved by ethephon in `Deshi' and `Early Bedana', by IBA in `Deshi' and `Purbi', and by 100 mm GA3 in all litchi cultivars. Cultivars responded differently to growth regulators, with `Deshi' responding significantly better than `Purbi', `Kasba', or `Early Bedana'. These studies point to the recalcitrant nature of litchi seeds. Chemical names used: gibberellic acid (GA3); indole butyric acid (IBA); 2-chloroethylphosphonic acid (ethephon).

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