scholarly journals Effects of calcium gluconate and ascorbic acid on controlling shoot necrosis during micropropagation of primocane-fruiting raspberry (Rubus idaeus L.) cultivars

2014 ◽  
Vol 13 (47) ◽  
pp. 4361-4368 ◽  
Author(s):  
Amalia Fridia ◽  
C. Debnath Samir ◽  
Rog Yeoung Young
Keyword(s):  
Ascorbic Acid ◽  
Calcium Gluconate ◽  
Rubus Idaeus ◽  
Shoot Necrosis

scholarly journals Influences of Postharvest Storage and Processing Techniques on Antioxidant and Nutraceutical Properties of Rubus idaeus L.: A Mini-Review

Horticulturae ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 105
Author(s):  
Ermes Lo Piccolo ◽  
Leani Martìnez Garcìa ◽  
Marco Landi ◽  
Lucia Guidi ◽  
Rossano Massai ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Frozen Storage ◽  
Chemical Compounds ◽  
Heat Pumps ◽  
Rubus Idaeus ◽  
Anthocyanin Content ◽  
Postharvest Storage ◽  
Hot Air ◽  
Berry Fruits ◽  
Processing Techniques

The growth of agricultural mechanization has promoted an increase in raspberry production, and for this reason, the best postharvest storage and processing techniques capable of maintaining the health beneficial properties of these perishable berry fruits have been widely studied. Indeed, raspberries are a rich source of bioactive chemical compounds (e.g., ellagitannins, anthocyanins, and ascorbic acid), but these can be altered by postharvest storage and processing techniques before consumption. Although there are clear differences in storage times and techniques, the content of bioactive chemical compounds is relatively stable with some minor changes in ascorbic acid or anthocyanin content during cold (5 °C) or frozen storage. In the literature, processing techniques such as juicing or drying have negatively affected the content of bioactive chemical compounds. Among drying techniques, hot air (oven) drying is the process that alters the content of bioactive chemical compounds the most. For this reason, new drying technologies such as microwave and heat pumps have been developed. These novel techniques are more successful in retaining bioactive chemical compounds with respect to conventional hot air drying. This mini-review surveys recent literature concerning the effects of postharvest storage and processing techniques on raspberry bioactive chemical compound content.

THE INHERITANCE OF ASCORBIC ACID IN RED RASPBERRY

1973 ◽  
Vol 15 (2) ◽  
pp. 372-375
Author(s):  
S. O. Fejer ◽  
F. B. Johnston ◽  
Madeleine M. Hammill
Keyword(s):  
Ascorbic Acid ◽  
Combining Ability ◽  
Rubus Idaeus ◽  
Red Raspberry ◽  
Leaf Sample ◽  
Combining Abilities ◽  
Fruit Samples

Combining ability for ascorbic acid was studied in fruit and leaves of red raspberry (Rubus idaeus, R. strigosus) progenies produced by crossing each of four cultivars with three other cultivars and their S2 selections. General combining abilities were significant in two fruit samples, but specific combining abilities in the second sample only. In the leaf sample, specific combining abilities were significant; but general combining abilities were not. General effects of the cultivars for fruit ascorbic acid were similar to previous effects on the cultivars themselves. Ascorbic acid levels of these crosses were higher than those of two parental clones grown in this experiment.

INFLUENCE DES CONCENTRATIONS HORMONALES, DU MILIEU DE CULTURE ET D’UN ANTI-OXYDANT SUR LE TEMPS DE DOUBLAGE DES TIGES DE FRAMBOISIERS ’MADAWASKA’ CULTIVES IN VITRO

1987 ◽  
Vol 67 (3) ◽  
pp. 863-869 ◽  
Author(s):  
Y. DESJARDINS ◽  
A. GOSSELIN
Keyword(s):  
Ascorbic Acid ◽  
Chlorophyll Content ◽  
Butyric Acid ◽  
Doubling Time ◽  
Rubus Idaeus ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Indole Butyric Acid ◽  
Doubling Times

Our results show that the shoot doubling time is a useful and accurate parameter to characterize the effect of media on the multiplication rate of tissue-cultured raspberry (Rubus idaeus L. ’Madawaska’). We have determined that 1 mg L−1 of BA (benzyladenine) and 0–0.05 mg L−1 IB A (indole butyric acid) resulted in the highest rates of proliferation, with shoot doubling times of 19 and 18 d for MS (Murashige and Skoog) and Anderson medium, respectively. A reduction of 10 d in the shoot doubling time was obtained with the addition of ascorbic acid (50 mg L−1) to both MS and Anderson media. However, this treatment did not reduce yellowing of cultures since the chlorophyll content was not significantly different in presence or absence of ascorbic acid. Generally, the MS medium was superior to Anderson medium.Key words: Raspberry, doubling time, multiplication in vitro, ascorbic acid, Rubus idaeus L.

Comparative Study of Ascorbic Acid Levels in Gastric Secretion, Blood, Urine and Saliva

1951 ◽  
Vol 18 (2) ◽  
pp. 224-229 ◽  
Author(s):  
Joseph T. Freeman ◽  
Roberta Hafkesbring
Keyword(s):  
Ascorbic Acid ◽  
Gastric Secretion ◽  
Comparative Study

Sorbitol enhances the physicochemical stability of B12 vitamers

2020 ◽  
Vol 90 (5-6) ◽  
pp. 439-447 ◽  
Author(s):  
Andrew Hadinata Lie ◽  
Maria V Chandra-Hioe ◽  
Jayashree Arcot
Keyword(s):  
Ascorbic Acid ◽  
Uv Light ◽  
Heat Exposure ◽  
Water Soluble ◽  
Uv Exposure ◽  
Physicochemical Stability ◽  
Before And After ◽  
The Stability

Abstract. The stability of B12 vitamers is affected by interaction with other water-soluble vitamins, UV light, heat, and pH. This study compared the degradation losses in cyanocobalamin, hydroxocobalamin and methylcobalamin due to the physicochemical exposure before and after the addition of sorbitol. The degradation losses of cyanocobalamin in the presence of increasing concentrations of thiamin and niacin ranged between 6%-13% and added sorbitol significantly prevented the loss of cyanocobalamin (p<0.05). Hydroxocobalamin and methylcobalamin exhibited degradation losses ranging from 24%–26% and 48%–76%, respectively; added sorbitol significantly minimised the loss to 10% and 20%, respectively (p < 0.05). Methylcobalamin was the most susceptible to degradation when co-existing with ascorbic acid, followed by hydroxocobalamin and cyanocobalamin. The presence of ascorbic acid caused the greatest degradation loss in methylcobalamin (70%-76%), which was minimised to 16% with added sorbitol (p < 0.05). Heat exposure (100 °C, 60 minutes) caused a greater loss of cyanocobalamin (38%) than UV exposure (4%). However, degradation losses in hydroxocobalamin and methylcobalamin due to UV and heat exposures were comparable (>30%). At pH 3, methylcobalamin was the most unstable showing 79% degradation loss, which was down to 12% after sorbitol was added (p < 0.05). The losses of cyanocobalamin at pH 3 and pH 9 (~15%) were prevented by adding sorbitol. Addition of sorbitol to hydroxocobalamin at pH 3 and pH 9 reduced the loss by only 6%. The results showed that cyanocobalamin was the most stable, followed by hydroxocobalamin and methylcobalamin. Added sorbitol was sufficient to significantly enhance the stability of cobalamins against degradative agents and conditions.

Inhibition of Pro-Inflammatory Cytokine Secretion by Select Antioxidants in Human Coronary Artery Endothelial Cells

2020 ◽  
Vol 90 (1-2) ◽  
pp. 103-112 ◽  
Author(s):  
Michael J. Haas ◽  
Marilu Jurado-Flores ◽  
Ramadan Hammoud ◽  
Victoria Feng ◽  
Krista Gonzales ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Ascorbic Acid ◽  
Coronary Artery ◽  
Inflammatory Cytokines ◽  
Inflammatory Cytokine ◽  
Culture Media ◽  
Cytokine Secretion ◽  
Human Coronary Artery ◽  
Tnf Α ◽  
Pro Inflammatory Cytokines

Abstract. Inflammatory and oxidative stress in endothelial cells are implicated in the pathogenesis of premature atherosclerosis in diabetes. To determine whether high-dextrose concentrations induce the expression of pro-inflammatory cytokines, human coronary artery endothelial cells (HCAEC) were exposed to either 5.5 or 27.5 mM dextrose for 24-hours and interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor α (TNF α) levels were measured by enzyme immunoassays. To determine the effect of antioxidants on inflammatory cytokine secretion, cells were also treated with α-tocopherol, ascorbic acid, and the glutathione peroxidase mimetic ebselen. Only the concentration of IL-1β in culture media from cells exposed to 27.5 mM dextrose increased relative to cells maintained in 5.5 mM dextrose. Treatment with α-tocopherol (10, 100, and 1,000 μM) and ascorbic acid (15, 150, and 1,500 μM) at the same time that the dextrose was added reduced IL-1β, IL-6, and IL-8 levels in culture media from cells maintained at 5.5 mM dextrose but had no effect on IL-1β, IL-6, and IL-8 levels in cells exposed to 27.5 mM dextrose. However, ebselen treatment reduced IL-1β, IL-6, and IL-8 levels in cells maintained in either 5.5 or 27.5 mM dextrose. IL-2 and TNF α concentrations in culture media were below the limit of detection under all experimental conditions studied suggesting that these cells may not synthesize detectable quantities of these cytokines. These results suggest that dextrose at certain concentrations may increase IL-1β levels and that antioxidants have differential effects on suppressing the secretion of pro-inflammatory cytokines in HCAEC.

Ascorbic Acid does not Improve Endothelium-Dependent Flow-mediated Dilatation of the Brachial Artery in Patients with Raynaud’s Phenomenon Secondary to Systemic Sclerosis

2003 ◽  
Vol 73 (1) ◽  
pp. 3-7 ◽  
Author(s):  
M. E. Mavrikakis ◽  
J. P. Lekakis ◽  
M. Papamichael ◽  
K. S. Stamatelopoulos ◽  
Ch. C. Kostopoulos ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Smooth Muscle ◽  
Systemic Sclerosis ◽  
Endothelial Dysfunction ◽  
Brachial Artery ◽  
Raynaud’S Phenomenon ◽  
Raynaud's Phenomenon ◽  
Water Soluble ◽  
Placebo Administration ◽  
Flow Mediated Dilatation

Previous studies have shown that patients with Raynaud’s phenomenon secondary to systemic sclerosis present abnormal endothelial function; the mechanisms responsible for the endothelial dysfunction are unknown but increased vascular oxidative stress could be a possible cause. The hypothesis that a potent water-soluble antioxidant can reverse endothelial dysfunction in these patients was tested in the present study. We examined 11 female patients with Raynaud’s phenomenon secondary to systemic sclerosis and ten healthy control women by ultrasound imaging of the brachial artery to assess flow-mediated (endothelium-dependent) and nitrate-induced (endothelium-independent) vasodilatation. Flow-mediated dilatation and nitrate-induced dilatation were significantly reduced in patients with Raynaud’s phenomenon, indicating abnormal endothelial and smooth muscle cell function. Patients with Raynaud’s phenomenon entered a double-blind, randomized, crossover placebo-controlled trial and received orally 2 g of ascorbic acid or placebo; vascular studies were repeated two hours after ascorbic acid or placebo administration. Flow-mediated dilatation did not improve after ascorbic acid (1.6 ± 2.2% to 2.2 ± 2.5%, ns) or placebo administration (1.2 ± 1,9% to 1.7 ± 1.4%, ns); also nitrate-induced dilatation was similar after ascorbic acid or placebo (16 ± 7.4% vs 17 ± 8%, ns), suggesting no effect of ascorbic acid on endothelial and vascular smooth muscle function. In conclusion, ascorbic acid does not reverse endothelial vasomotor dysfunction in the brachial circulation of patients with Raynaud’s phenomenon secondary to systemic sclerosis. The use of different antioxidants or different dosing of ascorbic acid may be required to show a beneficial effect on endothelial vasodilator function.

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