scholarly journals Antibacterial activity of endophytic fungi isolated from mangroves of Jaffna Peninsula, Sri Lanka

2020 ◽  
Vol 14 (6) ◽  
pp. 243-247
Author(s):  
Ravimannan N. ◽  
Sepali E.
Keyword(s):  
Antibacterial Activity ◽  
Sri Lanka ◽  
Endophytic Fungi

Revealing the endophytic mycoflora in tea (Camellia sinensis) leaves in Sri Lanka: the first comprehensive study

Phytotaxa ◽  
2021 ◽  
Vol 514 (3) ◽  
pp. 247-260
Author(s):  
KASUN THAMBUGALA ◽  
DINUSHANI DARANAGAMA ◽  
SAGARIKA KANNANGARA ◽  
THENUKA KODITUWAKKU
Keyword(s):  
Sri Lanka ◽  
Camellia Sinensis ◽  
Endophytic Fungi ◽  
Sequence Data ◽  
Fungal Endophytes ◽  
Fungal Endophyte ◽  
Tea Leaves ◽  
Molecular Sequence Data ◽  
Molecular Sequence ◽  
Rdna Sequence Data

Endophytic fungi are a diverse group of microorganisms that live asymptomatically in healthy tissues of host and they have been reported from all kinds of plant tissues such as leaves, stems, roots, flowers, and fruits. In this study, fungal endophytes associated with tea leaves (Camellia sinensis) were collected from Kandy, Kegalle, and Nuwara Eliya districts in Sri Lanka and were isolated, characterized, and identified. A total of twenty endophytic fungal isolates belonging to five genera were recovered and ITS-rDNA sequence data were used to identify them. All isolated endophytic fungal strains belong to the phylum Ascomycota and the majority of these isolates were identified as Colletotrichum species. Phyllosticta capitalensis was the most commonly found fungal endophyte in tea leaves and was recorded in all three districts where the samples were collected. This is the very first investigation on fungal endophytes associated with C. sinensis in Sri Lanka based on molecular sequence data. In addition, a comprehensive account of known endophytic fungi reported worldwide on Camellia sinensis is provided.

scholarly journals Isolation and Antibacterial Activity of Endophytic Fungi From Melochia umbellata (Houtt)

2017 ◽  
Vol 11 (3) ◽  
pp. 1313-1318
Author(s):  
Herlina Rante ◽  
Risfah Yulianty ◽  
Yayu Evary ◽  
Elvira Hardiana
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi

scholarly journals Antibacterial Activities of Endophytic Fungi Isolated from Mentha cordifolia Leaves and Their Volatile Constituents

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Sakon Monggoot ◽  
Jariya Burawat ◽  
Patcharee Pripdeevech
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
High Concentration ◽  
Phenyl Ethyl Alcohol ◽  
First Time

A total of 17 endophytic fungal isolates were obtained from the leaves of Mentha cordifolia Opiz (Lamiaceae). Seven isolates were identified to the level of genus by using taxonomically relevant morphological traits. Colletotrichum and Phomopsis species were dominant among these strains. All strains were separated from M. cordifolia leaf for the first time. The ethyl acetate extracts of all endophytic fungi were tested for antibacterial activity against Salmonella typhimurium TISTR1166 and Pseudomonas aeruginosa TISTR781. Most endophytes exhibited antibacterial activity. Ustilago sp. MFLUCC15-1024 presented the highest inhibition zone diameter with a MIC of 31.25 μg/mL against the tested pathogens. The chemical composition of the ethyl acetate extract of this strain was investigated using gas chromatography-mass spectrometry. Twenty-one components were identified. 2-Phenylethanol (38.7%), E-ligustilide (12.4%), α-eudesmol (10.2%), β-vetivone (4.6%), β-ylangene (3.7%) and verbanol (3.4%) were the major components of the extract. The strong antibacterial activity of Ustilago sp. MFLUCC15-1024 ethyl acetate extract may be attributed to the presence of a high concentration of bioactive compounds including phenyl ethyl alcohol, E-ligustilide and α-eudesmol. The results indicate that there is high diversity of endophytic fungi in M. cordifolia leaf, and that Ustilago sp. MFLUCC15-1024 strain could be an excellent resource of natural antibacterial compounds.

scholarly journals Antibacterial activity of endophytic fungi isolated from Croton lechleri (Euphorbiaceae)

2018 ◽  
Vol 12 (15) ◽  
pp. 170-178
Author(s):  
Luiz Pires Vargas Sebastiao ◽  
Kessyo Braga de Albuquerque Erlan ◽  
Albuquerque Lima Ribeiro Sandra ◽  
Vasconcelos de Araujo Atilon ◽  
Moura da Cunha Renildo ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Croton Lechleri

Antibacterial Activity of Traditional Medicine Scurrula atropurpurea (BL) DANS and their Endophytic Fungi

2020 ◽  
Vol 840 ◽  
pp. 205-213
Author(s):  
Elfita Elfita ◽  
Muharni Muharni ◽  
Mardiyanto Mardiyanto ◽  
Fitrya Fitrya ◽  
Feti Fera ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Bacterial Infections ◽  
Salmonella Typhi ◽  
Spectroscopy Analysis ◽  
Chromatography Method ◽  
Antibacterial Compounds ◽  
Activity Test ◽  
Antibacterial Compound ◽  
Promising Source

Scurrula atropurpurea known as benalu is a medicinal plant that has been used for the treatment of various diseases such as antibacterial. Plants with ethnomedicine history use to cure pathogenic bacterial infections and their endophytic fungi is a promising source of antibacterial compounds. This study aimed to compared the antibacterial activity of S. atropurpurea leaves and their endophytic fungi. The secondary metabolites were isolated from the leaves of S. atropurpurea and their endophytic fungi by the chromatography method. The antibacterial activity test was carried out by Kirby Bauer method against Salmonella typhi (IPCCCB.11.669) and Escherichia coli (ATCC 25922) as Gram (-) and Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923) as Gram (+). The antibacterial compound from S. atropurpurea was determined by spectroscopy analysis as Quercetin-3-O-α-L-Rhamnopyranoside, while the antibacterial compound from endophytic fungi (strain BB1) as a lactone. Phylogenetic tree of strain BB1 has the highest homology with Neopestalotiopsis surinamensis strain CBS 450.74.

scholarly journals ANTIBACTERIAL ACTIVITY OF ISOLATED ENDOPHYTIC FUNGI FROM RAUVOLFIA SERPENTINA (L.) BENTH. EX KURZ

2016 ◽  
Vol 8 (11) ◽  
pp. 38 ◽  
Author(s):  
Rajshree Sahu ◽  
Suneel Kumar ◽  
Ravindra Prasad Aharwal ◽  
Sardul Singh Sandhu
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Klebsiella Pneumoniae ◽  
Endophytic Fungi ◽  
Fungal Strain ◽  
Penicillium Citrinum ◽  
Rauvolfia Serpentina ◽  
Molecular Sequencing ◽  
Different Parts

Objective: The aim of the present study was to isolate the endophytic fungi from medicinal plant Rauvolfia serpentina (L.) Benth. ex Kurz. (Family Apocynaceae) and observed their antibacterial activity against bacteria as well as the molecular characterization of most potent fungal strain. Methods: Collection and isolation of endophytic fungi from different parts (root, shoot, leaves) of Rauvolfia serpentina plant. Screening of endophytic fungi for antibacterial activity was scrutinised against six bacteria viz. Bacillus subtilis, Enterococcus sp., Klebsiella pneumoniae, Escherichia coli, Salmonella typhimurium and Streptococcus pyogenes by using Agar well diffusion method. For molecular sequencing of potent fungi, the DNA was extracted, quantified and amplified by using two oligonucleotide primers ITS4 and ITS6 in PCR.Results: A total seven endophytic fungi Aspergillus niger, Penicillium citrinum, Cladosporium sp., Curvularia lunata, Aspergillus sp., Alternaria sp. and Aspergillus fumigatus were isolated from different parts of Rauvolfia serpentina and fungal strain Penicillium citrinum was shown the maximum zone of inhibition against Bacillus subtilis (23.0±0.12 mm), Escherichia coli (19.9±0.16 mm), Streptococcus pyrogens (19.2±0.59 mm), Enterococcus sp., (17.2±0.08 mm), Klebsiella pneumoniae (18.9±0.16 mm) and Salmonella typhimurium (15.1±0.16 mm). The molecular sequencing of the potent fungi was done by primers (ITS4 and ITS6) which showed strong specificity with fungal DNA and the percentages of identical matches of ITS4 and ITS6 DNA sequences in the GeneBank database (NCBI) were determined to 98 %.Conclusion: In the present study, the endophytic fungal strain Penicillium citrinumshowed the potential source of antibacterial bioactive compounds and molecular sequencing of this fungus helps in future to determine the various metabolic pathways that are responsible for the production of such type of novel compounds. 

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