scholarly journals Antibacterial Activities of Endophytic Fungi Isolated from Mentha cordifolia Leaves and Their Volatile Constituents

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Sakon Monggoot ◽  
Jariya Burawat ◽  
Patcharee Pripdeevech
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
High Concentration ◽  
Phenyl Ethyl Alcohol ◽  
First Time

A total of 17 endophytic fungal isolates were obtained from the leaves of Mentha cordifolia Opiz (Lamiaceae). Seven isolates were identified to the level of genus by using taxonomically relevant morphological traits. Colletotrichum and Phomopsis species were dominant among these strains. All strains were separated from M. cordifolia leaf for the first time. The ethyl acetate extracts of all endophytic fungi were tested for antibacterial activity against Salmonella typhimurium TISTR1166 and Pseudomonas aeruginosa TISTR781. Most endophytes exhibited antibacterial activity. Ustilago sp. MFLUCC15-1024 presented the highest inhibition zone diameter with a MIC of 31.25 μg/mL against the tested pathogens. The chemical composition of the ethyl acetate extract of this strain was investigated using gas chromatography-mass spectrometry. Twenty-one components were identified. 2-Phenylethanol (38.7%), E-ligustilide (12.4%), α-eudesmol (10.2%), β-vetivone (4.6%), β-ylangene (3.7%) and verbanol (3.4%) were the major components of the extract. The strong antibacterial activity of Ustilago sp. MFLUCC15-1024 ethyl acetate extract may be attributed to the presence of a high concentration of bioactive compounds including phenyl ethyl alcohol, E-ligustilide and α-eudesmol. The results indicate that there is high diversity of endophytic fungi in M. cordifolia leaf, and that Ustilago sp. MFLUCC15-1024 strain could be an excellent resource of natural antibacterial compounds.

scholarly journals GCMS ANALYSIS OF ENDOPHYTIC FUNGI CURVULARIA AERIA MTCC-12847 ISOLATED FROM TRIBULUS TERRESTRIS L.

2019 ◽  
pp. 26-32
Author(s):  
Kamana Sahani ◽  
DEEPENDRA THAKUR
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Active Compound ◽  
Ethyl Acetate Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Tribulus Terrestris ◽  
Chromatography Mass Spectrometry

Objective: The objective of the present investigation was to perform the Gas Chromatography-mass spectrometry (GCMS) analysis of endophytic fungi Curvularia aeria MTCC-12847 isolated from Tribulus terrestris L. to find out the active compound present in the extract. Methods: The endophytic fungi were isolated from the plant Tribulus Terrestris L., leaf which was cultivated in optimized media for the production of secondary metabolites and was extracted using ethyl acetate. Ethyl acetate extract was used for the Gas Chromatography-mass spectrometry (GCMS) analysis. Results: GC-MS analysis of ethyl acetate extract of endophytic fungi revealed the presence of various secondary metabolites, the highest amount present was Palmitic acid (24.54%) and Lowest was Dimethyl 1-phenyl-7-methyl-1-hydroxy-1,4-dihydronaphthalene-2,3-dicarboxylate (5.76%). Conclusion: The endophytic fungal Curvularia aeria MTCC-12847 extract isolated from the Tribulus terrestris L. shows the presence of various bioactive compounds.

scholarly journals Bioactive Compounds Isolated from Indonesian Epiphytic Plant of Sarang Semut and Their Antibacterial Activity against Pathogenic Oral Bacteria

2017 ◽  
Vol 12 (8) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Dikdik Kurnia ◽  
Dadan Sumiarsa ◽  
Hendra D.A. Dharsono ◽  
Mieke H. Satari
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Porphyromonas Gingivalis ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Steroid Glycoside ◽  
Oral Bacteria ◽  
Epiphytic Plant

A new phenolic compound (1), a steroid (2), a new steroid glycoside (4), two triterpenoids (3 & 6) and a new phloroglucinol-sesquiterpene (5) have been isolated from the ethyl acetate extract of Sarang Semut ( Myrmecodia pendans) and their structures were determined on the basis of the spectral data. The bioactivity evaluation was conducted with the inhibition zone of compounds (mm) using Kirby-Bauer method at concentrations of 1000 and 5000 ppm for compound 1 against pathogenic oral bacteria Enterococcus faecalis, was 8.55 and 8.05 mm, respectively. Compounds 2–3 against Streptococcus mutans were 9.00 and 8.45 mm (2) and 10.24 and 9.35 mm (3), respectively. Compound 5 against Porphyromonas gingivalis was 11.5 and 10.8 mm, respectively.

scholarly journals Antibacterial activity of endosymbiotic fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands, Jakarta

2021 ◽  
Vol 948 (1) ◽  
pp. 012069
Author(s):  
R Trifani ◽  
Noverita ◽  
T A Hadi ◽  
E Sinaga
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Marine Sponges ◽  
Disc Diffusion ◽  
Active Compounds ◽  
E Coli ◽  
Stylissa Carteri ◽  
Further Development

Abstract Endosymbiont fungi from marine sponges are a rich source of medicinally active compounds. Indonesia has a huge number of marine sponges. This research was conducted to determine the antibacterial potential of endosymbiont fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands. The screening was conducted with two methods, the modified GIBEX and disc diffusion. Species of marine sponges were isolated, namely Petrosia sp., Stylissa carteri, Cinachyrella australiensis, Callyspongia sp., Petrosia nigrians, and Stylissa massa, and obtained 9 isolates of endosymbiont fungi. The GIBEX test against Escherichia coli and Streptococcus mutans showed that ethyl acetate extract had strongest antibacterial activity. Disc diffusion test of ethyl acetate extract, five isolates had antibacterial activity against S. mutans and four against E. coli. The isolate that showed the strongest antibacterial activity was from Petrosia sp. Which has an inhibition zone of 8.4 mm against E. coli and 7.45 mm against S. mutans. The main active compounds from the isolate of Petrosia sp. are butylhydroxytoluene and phthalic acid di-(2-propylpentyl) esters. Based on this study, we concluded that the endosymbiont fungi of marine sponges are potential to be developed for further development as source of antibacterial agents.

scholarly journals UJI EFEKTIVITAS DAUN PANDAN WANGI (PANDANUS AMARYLLIFOLIUS ROXB.) SEBAGAI ANTIBAKTERI TERHADAP SALMONELLA TYPHI

2019 ◽  
Vol 6 (1) ◽  
pp. 59
Author(s):  
Priska Nancy Claudia Bali ◽  
Ahmad Raif ◽  
Setia Budi Tarigan
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Inhibition Zone Diameter ◽  
Negative Controls ◽  
Pandanus Amaryllifolius ◽  
Positive Controls

Pandanus amaryllifolius roxb leaves commonly used as food dditives which contain various compound groups namely alkaloid, saponins, flavonoids and tannins that have a role in antibacterial activity. This research is indicated to support the success of antibacterial in fragrant pandanus leaf plants against Salmonella typhi, one of the pathogenic bacteria. The antibacterial effectiveness test of ethanol-ethyl acetate extract (1: 1) of pandanus leaves which evaluated by the Kirby-bauer method and making the extract concentrations of 25%, 50%, 75%, 100%. The results showed that pandanus leaves extract had antibacterial effectiveness against Salmonella typhi with an average inhibition zone diameter of 11.6 mm; 14 mm; 14.3 mm; 15.3 mm; positive controls (Ciprofloxacin) 27 mm; and negative controls (aquadest) didn’t produce the inhibition zone of Salmonella typhi.

scholarly journals Screening of Antibacterial Activities of the Endophytic Fungi Isolated from the Leaves of Medinilla speciosa Blume

2021 ◽  
Vol 8 (3) ◽  
pp. 24-28
Author(s):  
Puteri Amelia ◽  
Rachma Ayunda ◽  
Saiful Bahri
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Bioactive Compound ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Shigella Dysenteriae ◽  
Bioactive Molecules ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Bacillus Subtilis Atcc

Endophytes or endophytic fungi have been investigated as a store house of bioactive compound. This study was aimed to evaluate the potential antibacterial activity of endophytic fungi isolated from Medinilla speciosa Blume. The antibacterial test was determined by measuring the inhibition zone with disk-diffusion method. Twenty endophytes were isolated from the leaves of Medinilla speciosa Blume and identified morphologically. The results demonstrated that ten isolates showed variation in their antibacterial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, and Shigella dysenteriae ATCC 13313. Further investigation will be needed to explore and identify the bioactive molecules of the isolated endophytic fungi.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TENGGULUN (Protium javanicum Burm. F.) TERHADAP BAKTERI Staphylococcus aureus

Jurnal Kimia ◽  
2020 ◽  
pp. 56
Author(s):  
N. M. Puspawati ◽  
N. L. P. F. Widiari ◽  
I M. Sukadana
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Spectra Analysis ◽  
Minimum Concentration ◽  
Butanol Extract ◽  
Leaf Powder ◽  
Inhibition Zones

This study aims to examine the antibacterial activity of n-hexane, ethyl acetate, and n-butanol extract of tenggulun leaves (Protium javanicum Burm. F.) in inhibiting the growth of S. aureus bacteria and determine the minimum inhibitory concentration of the most active extracts and identify its active compounds. A total of 1000 g of tenggulun leaf powder was macerated with methanol and 91.78 g of crude methanol extract was obtained which was further partitioned into n-hexane, ethyl acetate, and n-butanol extracts. Antibacterial activity test was carried out using the diffusion well method and the result showed that the ethyl acetate extract at a concentration of 20% was strongly able to inhibit the growth of S. aureus bacteria with inhibition zones of 20.08 mm, followed by n-butanol 16.66 mm, and n-hexane 13.33 mm. The minimum concentration of ethyl acetate extract was 0.2% in inhibiting the growth of S. aureus with inhibition zone diameter of 6.20 mm. Separation of the active ethyl acetate extract was conducted by silica gel column chromatography with mobile phase of n-hexane: ethyl acetate (7.5: 2.5) gave 6 combined fractions. Fractions B, G, H and I showed antibacterial activity at concentrations of 50% with a diameter of inhibition zone of 5.62 mm, 6.87 mm, 8.50 mm, and 6.75 mm respectively. The results of mass spectra analysis from the chromatogram peaks of LC-MS / MS suggested that the G and I fractions were tentatively identified as crotarin, benzophenone-2, and medroxyprogesterone acetate which may contributed to the antibacterial activity by acting synergisticly.    Keywords: antibacterial, tenggulun, Staphylococcus aureus, LC-MS/MS.

scholarly journals ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF MANGROVE Brugueira gymnorrhiza STEM EXTRACTS AGAINST PATHOGENIC BACTERIA Vibrio cholerae

2021 ◽  
Vol 3 (2) ◽  
pp. 53-61
Author(s):  
Angga Crystal Loasana Yami ◽  
Irmanida Batubara ◽  
Kholis Abdurachim Audah
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Vibrio Cholerae ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Ic50 Value ◽  
Dpph Method

Background : The treatment of some diseases caused by free radicals and pathogenic bacteria usually by using antioxidants and antibiotics. Due to excessive use of antibiotics and other environmental cues, some bacteria are now resistant to certain antibiotics or even to multiple antibiotics. Some Vibrio cholerae bacterial strains are multiresistant to many antibiotics.Objective : The antioxidant and antibacterial activities of Brugueira gymnorrhiza stem extracts against pathogenic bacteria V. cholerae.Method : The B. gymnorrhiza stem was extracted by gradient maceration method. The DPPH method was used to determine the antioxidant activity and the disc diffusion method was used to determine the antibacterial activities. The column chromatography method was used to fractionate the selective extract with the best activity. The LC-MS/MS method was used to identify the compound obtained from the fraction with the best antioxidant and antibacterial activity.Result : Ethyl acetate extract of B. gymnorrhiza stem had the best antibacterial activity with MIC and MBC values of 62.50 mg/L. Ethyl acetate extract also showed the best value of antioxidant activity as indicated by an IC50 value of 255.03 mg/L. The results of fractions test showed that fraction 3 had the best antibacterial and the best antioxidant activities with both the MIC and MBC values of 7.90 mg/L and IC50 value of 348.91 mg/L, respectively.Conclusion : Ethyl acetate extract of B. gymnorrhiza stem has good potential as antioxidant and antibacterial. The compound which is thought as antioxidant and antibacterial from Ethyl acetate extract is 2-Ethyl-4-methyl-1H-imidazole.

scholarly journals Antibacterial activity of endophytic fungi in Pometia pinnata against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Ratna Setyaningsih ◽  
Ari Susilowati ◽  
Dina Prasetyani
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Endophytic Fungi ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Its Region ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Bacterial Cells ◽  
Methicillin Resistant

Abstract. Endophytic fungi associated with medicinal plants are able to produce bioactive compounds which potentially contain medicinal ingredients. Matoa (Pometia pinnata J. R. Forst & G. Forst) is one of Indonesian medicinal plants. This research aims to test antibacterial activities of ethyl acetate extract of matoa’s endophytic fungi to Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA) and also identify isolates having high antibacterial activity. Initial test on antibacterial activity was conducted by using paper disc diffusion, while advanced test was conducted by determining minimum inhibitory concentration (MIC). The fungi were identified in morphological and molecular manner with amplification and sequencing of ITS region. Bacterial cells exposed to the extract were observed by using scanning electron microscope (SEM). Isolate AM72 was found out to have the highest antibacterial activity compared to other 12 isolates tested, with inhibition zone diameter of 31.57 mm to S. aureus and 22.87 mm to MRSA. Isolate AM72 was identified as Lasiodiplodia theobromae. Isolate BMB identified as Aspergillus oryzae produced inhibition zone with 21.96 mm diameter to S. aureus and 21.50 mm diameter to MRSA. Advanced test to isolate AM72 gave the same MIC results to S. aureus and MRSA of 1.56%. Antibacterial compound produced by isolate AM72 were found out to be able to damage bacterial cell walls. Keywords: Antibacterial, Endohpytic Fungi, Matoa, S. aureus, MRSA  

scholarly journals LC-MS/MS profiling, antibiofilm, antimicrobial and bacterial growth kinetic studies of Pluchea dioscoridis extracts

2021 ◽  
Author(s):  
Maha A.M. El-Shazly ◽  
Ahmed A. Hamed ◽  
Hoda A. Kabary ◽  
Mosad A. Ghareeb
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Incubation Period ◽  
Phenolic Acids ◽  
Ethyl Acetate Extract ◽  
Wide Spectrum ◽  
Water Extract ◽  
Inhibition Zone ◽  
Dependent Manner ◽  
Prolonged Incubation

Abstract The therapeutical applications of ornamental plants have been categorized to be of a great effectiveness in multiple industries from ancient times until present days. Pluchea dioscoridis is widely known Egyptian wooden plant that has been extensively applied for different medicinal purposes. In this study, LC-ESI-MS/MS analysis of the potent antimicrobial ethyl acetate and n-butanol extracts of P. dioscoridis leaves led to identification of 28 and 21 compounds, respectively. The identified compounds were categorized as phenolic acids, phenolic acids derivatives, organic acids, flavonoids (aglycones and glycosides), secoiridoids, coumarin derivatives, and gallotannins derivatives. Among them, caffeic acid 3-sulfate was the most predominate in the investigated extracts followed by ferulic acid and dicaffeoyl-quinic acid. Also, the antimicrobial potentiality of different extracts was evaluated against different pathogenic microbes including Enterobacter cloacae, Micrococcus leutus, Aeromonas hydrophila, Bacillus subtilis, Bacillus cereus, Bacillus lichneformis and Clostridium species. Furthermore, different concentrations of the most potent extract were assayed for antibacterial efficacy on growth curve kinetics against the susceptible bacteria along 4days incubation period. Our gathered data confirmed that, the antimicrobial activity against tested bacteria was different according to the solvent used in the extraction process. Mostly, all the extracts showed a wide spectrum antibacterial activity except the plant water extract which shows a mild activity against Clostridium sp. only. Based on the highest inhibition zone diameter, the ethyl acetate extract followed by butanol extract exhibited the highest inhibition zone with Micrococcus luteus and B. subtilis (20.0 and 18.5 mm) respectively. Determining the effect of ethyl acetate extract at different concentration (0, 0.66, 1.66, 3.33, 6.67, 13.34 and 20.01 mg mL−1) on M. luteus growth kinetics, the data assured that the antibacterial activity shows concentration dependent manner with the highest antibacterial activity at 20.01 mg mL−1 culture. The data also confirmed that, none of the selected concentration showed bactericidal activity in the prepared cultures, and with the prolonged incubation period the bacteria acquire resistance against the extract beginning from second or third day of incubation.

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