A comparison of scanning electron microscopy preparation methods to observe anatomical features and microbial changes during the vase life of Acacia and Rosa

2021 ◽  
Vol 282 ◽  
pp. 110039
Author(s):  
Virginia G. Williamson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Vase Life ◽  
Preparation Methods ◽  
Anatomical Features ◽  
Scanning Electron

Scanning electron microscopy of human iliac bone by a simple preparation method

1990 ◽  
Vol 48 (3) ◽  
pp. 226-227
Author(s):  
Toshihiko Takita ◽  
Tomonori Naguro ◽  
Toshio Kameie ◽  
Akihiro Iino ◽  
Kichizo Yamamoto
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Preparation Method ◽  
Specimen Preparation ◽  
Real Surface ◽  
Public Attention ◽  
Preparation Methods ◽  
The Real ◽  
Simple Preparation ◽  
Scanning Electron

Recently with the increase in advanced age population, the osteoporosis becomes the object of public attention in the field of orthopedics. The surface topography of the bone by scanning electron microscopy (SEM) is one of the most useful means to study the bone metabolism, that is considered to make clear the mechanism of the osteoporosis. Until today many specimen preparation methods for SEM have been reported. They are roughly classified into two; the anorganic preparation and the simple preparation. The former is suitable for observing mineralization, but has the demerit that the real surface of the bone can not be observed and, moreover, the samples prepared by this method are extremely fragile especially in the case of osteoporosis. On the other hand, the latter has the merit that the real information of the bone surface can be obtained, though it is difficult to recognize the functional situation of the bone.

Corrosion Behaviour of AZ91DSm1.0 with Different Preparation Methods

2014 ◽  
Vol 1004-1005 ◽  
pp. 113-118
Author(s):  
Hong Xia Liu ◽  
Jin Liang ◽  
Jue Zhang ◽  
Xiao Lian Zhang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Corrosion Resistance ◽  
Corrosion Behaviour ◽  
X Ray Diffraction ◽  
Vacuum Melting ◽  
Potentiodynamic Polarization Curve ◽  
Preparation Methods ◽  
X Ray ◽  
Scanning Electron

Corrosion behaviour of AZ91DSm1.0 with different preparation methods(vacuum melting and common melting) is investigated. X-ray diffraction(XRD), optical microscopy(OM), scanning electron microscopy(SEM), energy dispersive spectroscopy(EDS), corrosion weightless experiment and the potentiodynamic polarization curve are applied to characterize the corrosion behaviour. Results show that vacuum melting alloy possess higher corrosion resistance than common melting alloy. This is attributed to the rod-shaped Al3Sm which was formed in common melting alloy will have certain fragmentation effect on matrix that can lead to defects.

scholarly journals Contribution to the identification of the species Myrcia hatschbachii D. Legrand (Myrtaceae): anatomical and histochemical analyses

2021 ◽  
Vol 43 ◽  
pp. e75
Author(s):  
Larissa Junqueira Gatto ◽  
Vanessa Barbosa Bobek ◽  
Jane Manfron Budel ◽  
Natasha Tiemi Fabri ◽  
Josiane De Fatima Gaspari Dias ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phenolic Compounds ◽  
Vascular Bundle ◽  
Folk Medicine ◽  
Spongy Parenchyma ◽  
Anatomical Features ◽  
Arc Shape ◽  
Histochemical Tests ◽  
Scanning Electron

The genus Myrcia is used in folk medicine to treat diabetes. The plants used in folk medicine require morphological and anatomical references to attest to its authenticity. This is the first report of the microscopic study of Myrcia hatschbachii. In this sense, the aim of the present study was to describe the anatomical characteristics, in order to contribute to the species' identification. For anatomical and surface analyses free-hand sections were prepared and observed using optical microscopy, simultaneously some materials were processed and observed under scanning electron microscopy. In addition, histochemical tests were performed. The anatomical features described here correspond with previously reported features found in other species of the genus Myrcia. Furthermore, the following anatomical markers were observed in Myrcia hatschbachii: druses in the spongy parenchyma, concave-convex midrib, heart-shaped petiole, oval-shaped stem, C-shaped vascular bundle in the midrib and open arc shape with invaginated ends in the petiole; phenolic compounds in the phloem and lignified cells in the pith. The results of anatomical and histochemical analyses provide micromorphological and microchemical features that can help in the taxonomy and proper identification of the species.

A technique for preparing Beauveria spp. for scanning electron microscopy

1980 ◽  
Vol 58 (15) ◽  
pp. 1700-1703 ◽  
Author(s):  
E. C. Quattlebaum ◽  
G. R. Carner
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Freeze Drying ◽  
Osmium Tetroxide ◽  
Air Drying ◽  
Preparation Methods ◽  
Critical Point Drying ◽  
Scanning Electron

Vapor fixation for 96 h with 1% osmium tetroxide (OsO4) and 3–4 days air drying produced distortion-free specimens of Beauveria spp. for examination with the scanning electron microscope. A combination of 4 h OsO4 vapor fixation and freeze-drying also reduced disruption satisfactorily but specimens were not as well preserved as with the first method. Preparation methods that were ineffective in preventing collapse of hydrophilic structures were Cling Free® sprayed on specimens prior to examination, freeze-drying, critical-point drying (of unfixed material), and vapor fixation with glutaraldehyde.

scholarly journals Comparative structure of the osmophores in the flowers of Stanhopea graveolens Lindley and Cycnoches chlorochilon Klotzsch (Orchidaceae)

2012 ◽  
Vol 64 (2) ◽  
pp. 15-24 ◽  
Author(s):  
Mirosława Chwil
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Apical Part ◽  
Ornamental Plant ◽  
Abaxial Surface ◽  
Glandular Hairs ◽  
Anatomical Features ◽  
Cuticular Ornamentation ◽  
Comparative Structure ◽  
Scanning Electron

The genus <i>Tradescantia</i> comprises about 70 species. In Poland <i>Tradescantia x andersoniana</i> is basically grown as an ornamental plant that is recommended for borders and to be planted around garden ponds. The present study investigated flowering as well as the micromorphological and anatomical features of some floral elements of <i>Tradescantia x andersoniana</i> W. Ludw. Rohweder 'Karin'. The macro- and micromorphology of the flowers was examined using stereoscopic, light, and scanning electron microscopy (SEM). Spiderwort produces flowers with a diameter of 4.6-5 cm, which open early in the morning and close at noon. Non-glandular and glandular hairs grow on the abaxial surface of the calyx and on the apical part of the ovary. The glandular hairs develop a several-celled stalk and a unicellular spherical or elongated head. The staminal filaments produce chain-shaped trichomes. Striate cuticular ornamentation is found on their surface and on the epidermis covering the perianth. The striae on the cells of the stamen hairs run to the two poles of the cell. The pistil develops a wet stigma with unfused unicellular papillae. The cuticle on their surface is smooth, whereas on the style near the stigma it forms dense folds.

Scanning electron microscopy of flowering apices

1984 ◽  
Vol 42 ◽  
pp. 326-327
Author(s):  
Kit W. Lee
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Simple Technique ◽  
Charge Accumulation ◽  
Direct Examination ◽  
Shoot Apices ◽  
Plant Materials ◽  
Preparation Methods ◽  
Critical Point Drying ◽  
Scanning Electron

Critical point drying (CPD) is widely used for the preparation of delicate shoot apices for scanning electron microscopy (SEM). In order to prevent charge accumulation on the dehydrated specimen, it is necessary to coat the surface with metal by vacuum evaporation or sputtering. However, cellular shrinkage is the most common irritating artifact for the shoot apices thus prepared (1). In addition, specimen charging is a common occurrence during SEM observation.Direct examination of mature plant materials (e.g., leaf, stigma, pollen, etc.) in the SEM is one of the alternative preparation methods (2,3). Nevertheless, such a simple technique has not been commonly used for floral apices. The aim of the present investigation was to explore the feasibility of using unfixed, uncoated apices for SEM.

Biological Sample Preparation for SEM Imaging of Porcine Retina

2012 ◽  
Vol 20 (2) ◽  
pp. 28-31 ◽  
Author(s):  
Patrick Moran ◽  
Brittany Coats
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sample Preparation ◽  
Critical Point ◽  
Biological Sample ◽  
Environmental Scanning Electron Microscopy ◽  
Preparation Methods ◽  
Critical Point Drying ◽  
Sem Imaging ◽  
Scanning Electron

Sample preparation is a critical step in scanning electron microscopy (SEM) imaging. This is especially true for biological samples because of charge build-up and sensitivity to vacuum and electron beam damage. In terms of ultrastructure imaging, a variety of advancements in detectors and approaches have improved biological imaging such that fewer steps are required for sample preparation. However, the conventional approach incorporating osmium tetroxide fixing, ethanol dehydrating, critical-point drying, and coating still finds useful application. This paper evaluates three biological sample-preparation methodologies for imaging the ultrastructure of immature porcine retina. The three preparation methods examined are critical-point drying (CPD), hexamethyldisilazane (HMDS) dehydration, and direct imaging by environmental scanning electron microscopy (ESEM). Preparation methodologies were evaluated based on resulting image quality and reduced potential for artifacts.

scholarly journals Comparative morphology and anatomy of seeds of some Aethionema W.T. Aiton (Brassicaceae) taxa from Turkey

2019 ◽  
Vol 26 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Mehmet Cengiz Karaismailoğlu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Principal Component Analysis ◽  
Principal Component ◽  
Comparative Morphology ◽  
Seed Morphology ◽  
Anatomical Features ◽  
Major Significance ◽  
Surface Patterns ◽  
Scanning Electron

Seed morphology and anatomy are taxonomically significant in Brassicaceae. The seed structures of Aethionema from Turkey, which include 12 taxa (Aethionema syriacum, A. froedinii, A. arabicum, A. eunomioides, A. fimbriatum, A. speciosum subsp. speciosum, A. speciosum subsp. compactum, A. saxatile, A. oppositifolium, A. iberideum, A. armenum, A. grandiflorum) were studied for knowing seed morphological and anatomical features with one-way analysis of variance, cluster analysis and principal component analysis. Seed size, shape and color were examined with stereomicroscopy.The surface patterns of seed were observed using Scanning Electron Microscopy (SEM). In addition, structure, and thicknesses of testa and endosperm were investigated anatomically. Thickness of testa and endosperm were of major significance to illustrate interspecific relations among the examined taxa.

scholarly journals Structural features of flower trichomes in drug eyebright (Euphrasia stricta D. Wolff EX J. F. Lehm.)

2014 ◽  
Vol 66 (4) ◽  
pp. 35-44 ◽  
Author(s):  
Weronika Haratym ◽  
Elżbieta Weryszko-Chmielewska
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Glandular Trichomes ◽  
Folk Medicine ◽  
Structural Features ◽  
Raw Material ◽  
Wall Thickening ◽  
Anatomical Features ◽  
Ophthalmic Diseases ◽  
Scanning Electron

<em>Euphrasia stricta </em>D. Wolff ex J. F. Lehm. (Orobanchaceae) is a representative of plants that are widely used in folk medicine, phytomedicine, and homeopathy. The medicinal raw material derived from the drug eyebright is applied primarily in treatment of ophthalmic diseases. The investigations of trichomes in drug eyebright (<em>Euphrasia stricta </em>D. Wolff ex J. F. Lehm) were conducted in 2010–2011. Using light microscopy and scanning electron microscopy, their location and morphological and anatomical features were identified. Three types of non-glandular trichomes were found: short unicellular, long 1–2 celled, and long 2-celled with wall ornamentation. Additionally, 7 types of glandular trichomes were found; these included: unicellular clavate, 2–3-celled clavate, capitate with a unicellular head and a 3-cel- led stalk, capitate with a unicellular head and a 2-celled stalk, capitate with a 2-celled head, conical papillae, and ribbon-like trichomes with wall thickening.

Sign in / Sign up

Export Citation Format

Share Document