scholarly journals Evaluation of angiosuppressive activity of Pleurotus florida on developing chick embryo using chorioallantoic membrane assay

2021 ◽  
Vol 6 (1) ◽  
pp. 334-341
Author(s):  
RC Zacarias
Keyword(s):  
Chick Embryo ◽  
Chorioallantoic Membrane ◽  
Pleurotus Florida ◽  
Chorioallantoic Membrane Assay

scholarly journals THE INFLUENCE OF CORTISONE ON EXPERIMENTAL VIRAL INFECTION

1966 ◽  
Vol 123 (2) ◽  
pp. 309-325 ◽  
Author(s):  
K. Marilyn Smart ◽  
Edwin D. Kilbourne
Keyword(s):  
Chick Embryo ◽  
Inflammatory Response ◽  
Chorioallantoic Membrane ◽  
Allantoic Fluid ◽  
Influenza Viruses ◽  
Present System ◽  
Interferon Production ◽  
Hydrocortisone Administration ◽  
Experimental Viral Infection

A comparative study was undertaken of the pathogenesis of infection of the allantoic sac of the chick embryo with three influenza viruses of differing virulence, and of the influence of hydrocortisone on the course of infection. Judged on the basis of earlier onset and greater degree of inflammatory response and diminished survival time of infected embryos, Mel. and Lee viruses were markedly more virulent than PR8, despite the earlier appearance of virus in PR8-infected embryos. Interferon appeared first and in greater quantity in the allantoic fluid of Lee-infected embryos and latest with PR8 infection. Thus, there was no correlation of avirulence and better interferon production with the viruses under study in the present system. Furthermore, evidence obtained suggested that Lee virus ("virulent") was most susceptible to interferon action, and also that viral synthesis in the chorioallantoic membrane with PR8 ("avirulent") persisted after the appearance of interferon. The injection of hydrocortisone within 2 hr of the initiation of infection delayed the synthesis of all three viruses; had no significant effect upon the inflammatory response; and transiently inhibited the synthesis of interferon, while prolonging the survival of Lee- and Mel.-infected embryos. Late administration of hydrocortisone suppresses both the inflammatory response and the production of interferon. Only in the case of Lee virus infection did hydrocortisone administration lead to augmentation of final yields of virus with the low infection multiplicity employed in the present experiments. It is postulated that Lee virus is a better inducer of interferon because its infectivity in vivo is more rapidly inactivated. As a consequence synthesis of Lee virus is more under the control of endogenous interferon than is the case with PR8 or Mel. virus. Therefore, inhibition of interferon synthesis with hydrocortisone has a greater influence on final yields of Lee virus.

Responses of tumour cell lines implanted onto the chorioallantoic membrane of chick embryo to anticancer agents in combination with hyperthermia

1992 ◽  
Vol 20 (3) ◽  
pp. 237-239 ◽  
Author(s):  
T. Uchibayashi ◽  
M. Egawa ◽  
K. Nakajima ◽  
H. Hisazumi ◽  
M. Tanaka ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Cell Lines ◽  
Anticancer Agents ◽  
Tumour Cell ◽  
Chorioallantoic Membrane ◽  
Tumour Cell Lines

scholarly journals The growth in vitro of vaccinia virus in chick embryo chorio-allantoic memberane, minced embryo and cell suspensions

1957 ◽  
Vol 55 (3) ◽  
pp. 347-360 ◽  
Author(s):  
H. B. Maitland ◽  
D. I. Magrath
Keyword(s):  
Chick Embryo ◽  
Vaccinia Virus ◽  
Growth Curve ◽  
Chorioallantoic Membrane ◽  
Growth Cycle ◽  
Cell Suspensions ◽  
Rabbit Skin ◽  
Chick Chorioallantoic Membrane ◽  
Considerable Doubt

The growth curve of rabbit skin-adapted vaccinia virus in the chick chorioallantoic membrane incubated in Hanks' solution showed a drop in titre of virus for about 10 hr. followed by growth. At least 25% of virus, sometimes more, remained infective. A similar fall in titre was observed in heated membranes in which the virus did not grow and this occurred also when membranes, either normal or heated, were infected and disintegrated before incubation.The growth curve of virus in minced chick-embryo was similar to that in chorioallantoic membrane.Virus in cell suspensions prepared from chick embryo and incubated in a nutrient medium showed only a small loss of infectivity before growth in some experiments and rarely dropped below 65–70 % of the original titre in others.These results throw considerable doubt on the view that loss of infectivity preceding growth of vaccinia virus should be interpreted as an essential part of a growth cycle.

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