In-vitro Antimicrobial Susceptibility of Clostridium perfringens Isolates from Meat

2018 ◽  
Vol 20 (2) ◽  
pp. 137
Author(s):  
Shakuntala Birla ◽  
R.V. Singh ◽  
Bhavana Gupta ◽  
Kashikar Supriya Satish ◽  
A.P. Singh ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Antimicrobial Susceptibility

scholarly journals In Vitro Antimicrobial Susceptibility of Mycobacterium abscessus in Korea

2008 ◽  
Vol 23 (1) ◽  
pp. 49 ◽  
Author(s):  
Sunghoon Park ◽  
Shinok Kim ◽  
Eun Mi Park ◽  
Hojoong Kim ◽  
O Jung Kwon ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Mycobacterium Abscessus

scholarly journals Innovative and Highly Sensitive Detection of Clostridium perfringens Enterotoxin Based on Receptor Interaction and Monoclonal Antibodies

Toxins ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 266
Author(s):  
Thea Neumann ◽  
Maren Krüger ◽  
Jasmin Weisemann ◽  
Stefan Mahrhold ◽  
Daniel Stern ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Clostridium Perfringens ◽  
Food Poisoning ◽  
Receptor Interaction ◽  
Clostridium Perfringens Enterotoxin ◽  
Fast Detection ◽  
Highly Sensitive ◽  
Basic And Applied Research ◽  
Highly Sensitive Detection

Clostridium perfringens enterotoxin (CPE) regularly causes food poisoning and antibiotic-associated diarrhea; therefore, reliable toxin detection is crucial. To this aim, we explored stationary and mobile strategies to detect CPE either exclusively by monoclonal antibodies (mAbs) or, alternatively, by toxin-enrichment via the cellular receptor of CPE, claudin-4, and mAb detection. Among the newly generated mAbs, we identified nine CPE-specific mAbs targeting five distinct epitopes, among them mAbs recognizing CPE bound to claudin-4 or neutralizing CPE activity in vitro. In surface plasmon resonance experiments, all mAbs and claudin-4 revealed excellent affinities towards CPE, ranging from 0.05 to 2.3 nM. Integrated into sandwich enzyme-linked immunosorbent assays (ELISAs), the most sensitive mAb/mAb and claudin-4/mAb combinations achieved similar detection limits of 0.3 pg/mL and 1.0 pg/mL, respectively, specifically detecting recombinant CPE from spiked feces and native CPE from 30 different C. perfringens culture supernatants. The implementation of mAb- and receptor-based ELISAs into a mobile detection platform enabled the fast detection of CPE, which will be helpful in clinical laboratories to diagnose diarrhea of assumed bacterial origin. In conclusion, we successfully employed an endogenous receptor and novel high affinity mAbs for highly sensitive and specific CPE-detection. These tools will be useful for both basic and applied research.

scholarly journals Association of Antimicrobial Susceptibility and Treatment Outcome in Acne Vulgaris Patients: A Pilot Study

2020 ◽  
Vol 12 (04) ◽  
pp. 233-238
Author(s):  
Ashvini K. Yadav ◽  
Suneel Bhooshan ◽  
Allen Johnson ◽  
Dinesh P. Asati ◽  
Shashwati Nema ◽  
...  
Keyword(s):  
Treatment Outcome ◽  
Antimicrobial Susceptibility ◽  
Acne Vulgaris ◽  
Tertiary Care ◽  
Care Hospital ◽  
In Vitro Susceptibility ◽  
Persistent Infections ◽  
Significant Difference ◽  
Cutibacterium Acnes

Abstract Purpose Cutibacterium acnes (C. acnes) is an emerging pathogen that is highly resistant to antibiotics and is capable of causing persistent infections that are difficult to treat. Methods & Materials Acne vulgaris patients visiting dermatology OPD of our tertiary care hospital during the study period of 2 months were recruited. Skin swabs were collected, and the sample was processed on 5% sheep-blood agar for anaerobic culture by the GasPak method. Isolates were identified by the standard biochemical test. Antimicrobial susceptibility testing was performed for clinically relevant antibiotics by the E-strip method. The clinical response was evaluated after 1-month follow-up to the prescribed antibiotics. Results Minocycline, doxycycline, ceftriaxone, and tetracycline were the most effective antibiotics. Nonsusceptibility to clindamycin and erythromycin were observed in 11.9% and 31% isolates, respectively, with 9.5% isolates being nonsusceptible to both. For none of the antibiotics we found significant difference in the proportion of susceptible and nonsusceptible isolates between mild, moderate, and severe grades of acne vulgaris. For none of the antibiotic regimens, significant difference was observed between nonresponders and responders. Twenty-seven patients received clindamycin and among them 16 of 19 responders and 6 of 8 nonresponders yielded growth of clindamycin-susceptible isolates (p = 0.57). Conclusion We observed significant prevalence of resistant strains of C. acnes among patients with acne vulgaris. No association was observed between in vitro susceptibility results and treatment outcome.

In vitro antimicrobial susceptibility of glycopeptide-resistant enterococci

1995 ◽  
Vol 21 (1) ◽  
pp. 47-50 ◽  
Author(s):  
Collin Freeman ◽  
Ann Robinson ◽  
Brian Cooper ◽  
Mary Mazens-Sullivan ◽  
Richard Quintiliani ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility

scholarly journals Yersinia enterocolitica: In Vitro Antimicrobial Susceptibility

1978 ◽  
Vol 13 (5) ◽  
pp. 888-890 ◽  
Author(s):  
M. Raevuori ◽  
S. M. Harvey ◽  
M. J. Pickett ◽  
W. J. Martin
Keyword(s):  
Yersinia Enterocolitica ◽  
Antimicrobial Susceptibility

scholarly journals Etiology and in vitro antimicrobial susceptibility profile of strains isolated from bovine mastitis in dairy herds from the midwest region of Santa Catarina state, Brazil

2017 ◽  
Vol 11 (4) ◽  
pp. 219-225
Author(s):  
Mylena Karoline Valmorbida ◽  
Danielle Correia dos Santos Carneiro ◽  
Keila Catarina Prior ◽  
Eliete Griebeler ◽  
Marcella Zampoli Troncarelli ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Bovine Mastitis ◽  
Dairy Herds ◽  
Santa Catarina ◽  
Antimicrobial Susceptibility Profile ◽  
Midwest Region ◽  
Santa Catarina State

scholarly journals Gentamicin susceptibility among a sample of multi-drug resistantNeisseria gonorrhoeaeisolates in India

2016 ◽  
pp. AAC.01907-16 ◽  
Author(s):  
Manju Bala ◽  
Vikram Singh ◽  
Aradhana Bhargava ◽  
Monika Kakran ◽  
Naveen Chandra Joshi ◽  
...  
Keyword(s):  
Effective Treatment ◽  
Treatment Outcomes ◽  
Treatment Option ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Dual Therapy ◽  
Clinical Treatment ◽  
Antimicrobial Susceptibility Testing ◽  
Effective Treatment Option

Antimicrobial susceptibility testing of 258N. gonorrhoeaeisolates by Etest determined that 60.1% were MDR while 5% strains had decreased susceptibility to currently recommended extended-spectrum cephalosporins (ESCs). Among these, 84.5% MDR and 76.9% strains having decreased susceptibility to ESCs were susceptible to gentamicin. No MDR isolate was resistant to gentamicin. Thesein vitroresults suggest that gentamicin might be an effective treatment option for the MDR strains and in dual therapy for gonorrhea. However, further research regarding the clinical treatment outcomes is essential.

scholarly journals Antimicrobial susceptibility of Clostridium perfringens strains isolated from broiler chickens

2009 ◽  
Vol 40 (2) ◽  
pp. 262-264 ◽  
Author(s):  
R. O. S. Silva ◽  
F.M. Salvarani ◽  
R.A. Assis ◽  
N.R.S. Martins ◽  
P.S. Pires ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Antimicrobial Susceptibility ◽  
Broiler Chickens

In vitro antimicrobial susceptibility of anaerobic bacteria isolated from caprine footrot

2007 ◽  
Vol 55 (1) ◽  
pp. 11-20 ◽  
Author(s):  
Angela Lacombe-Antoneli ◽  
S. Píriz ◽  
S. Vadillo
Keyword(s):  
Antibiotic Treatment ◽  
Antimicrobial Susceptibility ◽  
Antimicrobial Agents ◽  
Anaerobic Bacteria ◽  
Gram Negative ◽  
Western Spain ◽  
Clinical Cases

The in vitro antimicrobial susceptibility of Gram-negative anaerobic bacilli commonly isolated from footrot in goats was studied. A total of 97 isolates belonging to the genera Dichelobacter, Fusobacterium, Prevotella, Porphyromonas and Bacteroides, obtained from clinical cases of footrot in south-western Spain between March 2000 and May 2001, were tested against 25 antimicrobial agents comprising β-lactams, aminoglycosides, macrolides, chloramphenicol, quinolones, lincosamides, sulphonamides and tetracyclines in order to optimise antibiotic treatment of this disease in goats. β-lactams, tetracyclines and metronidazole displayed the highest in vitro efficacy against the species involved in the pathogenesis of footrot.

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