Evaluation of Chokuwa Paddy (Semi glutinous) Landraces of Assam for Nutritive Values and Genetic Diversity Based on Seed Protein Profile by SDS-PAGE

2018 ◽  
Vol 31 (1) ◽  
pp. 64
Author(s):  
Karabee Dutta ◽  
Gautam K Handique ◽  
AK Handique
Keyword(s):  
Genetic Diversity ◽  
Seed Protein ◽  
Protein Profile ◽  
Sds Page ◽  
Nutritive Values

scholarly journals Evaluation of Genetic Diversity of Seven Species of Solanum in Nigeria Using SDS-PAGE

2019 ◽  
pp. 1-6
Author(s):  
Busirat Odunayo Ogundairo ◽  
Muyiwa Segun Ayodele
Keyword(s):  
Genetic Diversity ◽  
Gel Electrophoresis ◽  
Seed Protein ◽  
Protein Profile ◽  
Generic Status ◽  
Molecular Weights ◽  
Sds Page ◽  
The Family ◽  
Intraspecific Relationships ◽  
Poly Acrylamide

Seed protein profile of 12 accessions belonging to 7 species of the genus Solanum of the family solanaceae were investigated through poly acrylamide gel electrophoresis. The accessions were selected based on their overlapping morphological associations observed on the field. Occurrence of two protein bands of molecular weights 100 kda and 20 kda in all accession showed interspecific relationships and could be used as generic bands among the genus Solanum. All morphologically similar accessions manifested similarities in their protein bands except S. melongena. Dendrogram based on upgma also revealed the generic status and intraspecific relationships of the accession of S. aethiopicum and S. macrocarpon.

scholarly journals Protein Profile Study of Some Nigerian Sesame (Sesamum indicum L.) Accessions

2015 ◽  
Vol 3 (2) ◽  
pp. 322-329 ◽  
Author(s):  
Gbenga Olorunshola Alege
Keyword(s):  
Genetic Diversity ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Sesamum Indicum ◽  
Genomic Changes ◽  
Sds Page ◽  
Sesame Genotypes ◽  
Species Specific ◽  
Total Seed

This study was carried out to investigate the genetic diversity among 23 sesame (Sesamum indicum L.) accessions obtained from different agro-ecological localities from 10 different states across 4 geopolitical zones in Nigeria using evidence from Sodium Dodecyl Polyacrylamide Gel Electrophoresis (SDS-PAGE). Total seed protein of the studied plants resolved on 12% SDS-PAGE showed variations in numbers and intensity of bands among the different sesame accessions. Thirteen (13) major bands were recorded in this study. Lack of unique band and presence of common band (band 7) among the 23 studied sesame accessions indicate some levels of genetic affinity and evidence of common evolutionary origin of the sesame genotypes. This band can therefore be tagged as species specific band for discriminating Sesamum indicum. Cluster analysis grouped the 23 sesame genotypes into two clusters with similarity coefficient ranging from 0.42 to 0.96 which indicates existence of genetic diversity; therefore there is ample opportunity for improving the 23 sesame genotypes. Variations in protein bands observed among the 23 studied plants could be attributed to genomic changes taken place during species diversification. It can be concluded that genetic diversity existed among Nigerian sesame for the improvement of characters of interest. Accessions 9 (YOL), 15(OTT), 22 (OFF) and 23 (JAL) are therefore recommended for used in future breeding programs for the development of improved sesame varieties.Int J Appl Sci Biotechnol, Vol 3(2): 322-329 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12734

Studies on genetic diversity in chickpea utilizing morphological and total seed protein markers

2015 ◽  
Author(s):  
V. Jayalakshmi ◽  
C. Kiran Kumar Reddy ◽  
G. Jyothirmayi ◽  
A. Trivikrama Reddy
Keyword(s):  
Genetic Diversity ◽  
Dna Markers ◽  
Seed Protein ◽  
Storage Proteins ◽  
Protein Profile ◽  
Similarity Index ◽  
Morphological Characters ◽  
Morphological Markers ◽  
Protein Markers ◽  
Total Seed

Genetic diversity among seventeen chickpea varieties was investigated utilizing agro morphological traits and total seed protein markers. Morphological markers grouped all the desi genotypes (9) except ICCV 10 under Cluster I, whereas kabuli genotypes (7) were grouped in three clusters. Dendogram developed based on total seed protein profile revealed that the seventeen genotypes could be grouped into two clusters broadly at a distance of 25. Similarity index between all possible pairs of seventeen genotypes showed a range of 0.074 (Vishal and ICCV-10) to 0.579 (JAKI-9218 and ICCC 37). The correspondence between different methods might be improved by analyzing more morphological characters, storage proteins and DNA markers.

scholarly journals Genetic variations in Turkey cultivar and ecotype Medicago sativa species: cytological, total protein profile, and molecular characterization

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Büşra Yazıcılar ◽  
Gholamreza Jannati ◽  
Ismail Bezirganoglu
Keyword(s):  
Genetic Diversity ◽  
Flow Cytometry ◽  
Medicago Sativa ◽  
Cytogenetic Analysis ◽  
Nutritional Value ◽  
Protein Profile ◽  
Control Plant ◽  
Perennial Plant ◽  
Sds Page ◽  
Simple Sequence

Abstract Background Alfalfa(Medicago sativa L.) is a perennial plant, which is high in nutritional value and resistant to environmental conditions, and it is one of most frequently preferred feed crop among the leguminous family. In this study, it was aimed to determine the genetic diversity of some alfalfa ecotypes and their varieties by DNA, protein, nucleus, and chromosome counts. The genetic distance between the populations of control (M. truncatula), five different cultivars (Alsancak, Bilensoy, Iside, Plato, Bilensoy82), and three different ecotypes (Erzurum, Muş, and Konya) was investigated by cytogenetic analysis, flow cytometry, simple sequence repeats (SSR), and SDS PAGE techniques. Results Cytogenetic analysis of these tested plants has verified the existence of expected levels such as diploid, triploid, and tetraploid as well as aneuploid (2n = 4x = 30) plants. Flow cytometry analysis have displayed that all of tested plants were tetraploid, whereas cytological analysis had either diploid, triploid, or tetraploid. Genetic diversity dendrogram was created using Erzurum, Muş, Konya, Bilensoy82, Alsancak, and Plato varieties. The Iside and Bilensoy were found to be morphogenetic in relationship. Our control plant, M. truncatula, did not have a similarity relationship with other ecotypes and cultivars. The total numbers of protein bands differed among tested plants from 140 kDA to 25 kDa. Conclusions This paper first reports on the genetic variation of Turkish alfalfa plants by using detailed analysis techniques. This work provides important findings for the classification, conservation, and innovation of alfalfa germplasm resources.

scholarly journals Revealing Antioxidant and Antidiabetic Potency of Melinjo (Gnetum Gnemon) Seed Protein Hydrolysate at Different Stages of Seed Maturation

2019 ◽  
Vol 7 (2) ◽  
pp. 479-487 ◽  
Author(s):  
Anang Supriyadi ◽  
Laras Sekar Arum ◽  
Ari Satia Nugraha ◽  
Anak Agung Istri Ratnadewi ◽  
Tri Agus Siswoyo
Keyword(s):  
Seed Protein ◽  
Protein Hydrolysate ◽  
Protein Profile ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Antidiabetic Activity ◽  
Degree Of Hydrolysis ◽  
Protein Extract ◽  
Sds Page ◽  
Gnetum Gnemon

roteins hydrolyzed from melinjo seeds (Gnetum gnemon) at green (GM), yellow (YM) and red (RM) stages of maturity were studied for their effectiveness in antioxidant and antidiabetic activities. The seed protein extract was hydrolyzed using alcalase 2.4L, and the resulting hydrolysates with the highest degree of hydrolysis, protein profile, and the most potent contributors to antioxidant and invitro antidiabetic activities were identified. The degree of hydrolysis value of hydrolysates ranged from 52-84%, and the SDS-PAGE protein profile showed two distinct bands in which the band with molecular weight of 30 kDa degraded more intensively. Antioxidant capacity was measured using different standard methods, including radical cation 2,2-azinobis-(3-ethylbenzothizoline-6-sulphonate) (ABTS•+) assay, hydroxyl radical (OH•), and superoxide anion (O2•-) scavenging. The green hydrolysate (GMH) had significantly higher (p<0.05) free radical scavenging (ABTS•+, OH•, and O2•-) activities than that of the yellow hydrolysate (YMH) and red hydrolysate (RMH). However, invitro antidiabetic testing was performed based on the inhibitory activity of α-amylase and α-glucosidase. GMH was found to be more effective than YMH and RMH. These results showed that the antioxidant and antidiabetic activity in hydrolyzed GM protein has high potential to be utilized as natural nutraceuticals.

scholarly journals Genetic diversity and phylogenetic relationship among Anabantoidei fish (Anabantiformes) in South Kalimantan, Indonesia based on SDS-PAGE analysis

2019 ◽  
Vol 20 (9) ◽  
Author(s):  
Rani Sasmita ◽  
Mabrur Mabrur ◽  
UMMY SHALIHA AULIA RAHMY ◽  
Badruzsaufari Badruzsaufari
Keyword(s):  
Genetic Diversity ◽  
Ammonium Sulfate ◽  
Phylogenetic Relationship ◽  
Soluble Protein ◽  
Phylogenetic Trees ◽  
Protein Profile ◽  
Phylogenetic Reconstruction ◽  
Fish Muscle ◽  
Freshwater Ecosystem ◽  
Sds Page

Abstract. Sasmita R, Mabrur, Rahmy USA, Badruzsaufari. 2019. Genetic diversity and phylogenetic relationship among Anabantoidei fish (Anabantiformes) in South Kalimantan, Indonesia based on SDS-PAGE analysis. Biodiversitas 20: 2519-2527. Protein profile is a molecular marker for diversity and phylogenetic analysis of germplasm, including Anabantoidei fish which is abundant in freshwater ecosystem of South Kalimantan. The aim of this research was to analyze genetic diversity and construct phylogenetic relationship among Anabantoidei fish based on SDS PAGE analysis. Protein was extracted from muscle tissue of six species of Anabantoidei fish and precipitated using Ammonium sulfate salt. Soluble protein content was determined using Bradford assay and then separated based on SDS-PAGE method. Genetic diversity and phylogenetic relationship were constructed using PAST software based on UPGMA method. The results showed that the soluble protein of fish muscle can be precipitated optimally in different ammonium sulfate concentration. Based on SDS-PAGE analysis, 59 different protein bands have been separated from gels with molecular weight ranging from 28.15 to 181.61 kDa. On the protein level, the Anabantoidei fish showed high genetic polymorphism (greater than 90%) with 3, 2 and 5 monomorphic bands on non-precipitated, AS-precipitated and combination between non- and AS-precipitated proteins, respectively. The phylogenetic reconstruction also exhibited that the Anabantoidei fish has the unique phylogenetic trees, especially for the combined protein datasets. This information would be useful for freshwater fish conservation and breeding programs.

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