Studies on genetic diversity in chickpea utilizing morphological and total seed protein markers

2015 ◽  
Author(s):  
V. Jayalakshmi ◽  
C. Kiran Kumar Reddy ◽  
G. Jyothirmayi ◽  
A. Trivikrama Reddy
Keyword(s):  
Genetic Diversity ◽  
Dna Markers ◽  
Seed Protein ◽  
Storage Proteins ◽  
Protein Profile ◽  
Similarity Index ◽  
Morphological Characters ◽  
Morphological Markers ◽  
Protein Markers ◽  
Total Seed

Genetic diversity among seventeen chickpea varieties was investigated utilizing agro morphological traits and total seed protein markers. Morphological markers grouped all the desi genotypes (9) except ICCV 10 under Cluster I, whereas kabuli genotypes (7) were grouped in three clusters. Dendogram developed based on total seed protein profile revealed that the seventeen genotypes could be grouped into two clusters broadly at a distance of 25. Similarity index between all possible pairs of seventeen genotypes showed a range of 0.074 (Vishal and ICCV-10) to 0.579 (JAKI-9218 and ICCC 37). The correspondence between different methods might be improved by analyzing more morphological characters, storage proteins and DNA markers.

scholarly journals Genetic diversity in some Nigerian kenaf (Hibiscus cannabinus L.) genotypes as assessed by agro-morphological traits and seed protein markers

2019 ◽  
Vol 36 (1) ◽  
pp. 177
Author(s):  
M.A. Ayo-Vaughan ◽  
J.B.O. Porbeni ◽  
C.O. Alake ◽  
O.A. Oduwaye ◽  
E.O. Eigbe
Keyword(s):  
Genetic Diversity ◽  
Morphological Traits ◽  
Seed Protein ◽  
Hibiscus Cannabinus ◽  
Protein Markers

scholarly journals Assessment of the Genetic Diversity of Joha Rice Germplasms by using Simple Sequence Repeat Markers

2021 ◽  
Author(s):  
P. Saikia ◽  
B. Neog ◽  
N. Gogoi ◽  
D. Baruah
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Genetic Relationship ◽  
Simple Sequence Repeat ◽  
Similarity Index ◽  
Morphological Characters ◽  
Aromatic Rice ◽  
Sequence Repeat ◽  
Rice Landraces ◽  
Simple Sequence

Background: Joha Rice are aromatic rice landraces, having small to medium grain size, indigenous to Assam, India. Due to the introduction of high yielding hybrid varieties, many endemic rice landraces including Joha Rice, are in a verge of extinction, as these can only be conserved and maintained by repetitive cultivation. As there is a conflict of local names for these landraces, many landraces with similar morphological characters have been reported from various parts. Simple sequence repeat (SSR) markers with longer perfect repeats have earlier proved successful and essential in studying the genetic diversity among rice cultivars. The present study is aimed to evaluate the genetic relationship among fifteen (15) aromatic Joha rice landraces endemic to Upper Brahmaputra Valley, Assam.Methods: In the present investigation, different landraces of Joha rice were surveyed during 2016-2019. 15 landraces were selected, based on their morphological characters and local data. The collected germplasm of Joha rice was grown in the experimental plots and DNA from young, healthy leaves were isolated which were further used for determination of genetic diversity using SSR markers. Thirty-eight SSR markers were used to evaluate the genetic relationship among the fifteen aromatic rice landraces.Result: A total of 110 polymorphic alleles were detected by 34 markers across all the landraces, with an average of 3.25 per locus. The Polymorphic Information Content (PIC) ranged from 0.24 to 0.83, with an average of 0.5 for each marker. The marker RM154, RM454 and RM489 produced maximum six alleles showing PIC value of 0.82, 0.82 and 0.83, indicating a high polymorphism. UPGMA cluster analysis using Jaccard’s similarity index produced a dendrogram clustering the rice landraces in three major groups and five subgroups. Group II, which consisted of five sub-groups and 12 landraces, showed diverse genotypes. These landraces showed significant genetic similarities. 

scholarly journals Genetic diversity of Polish cultivars of common wheat (Triticum aestivum L.) based on molecular and protein markers

2020 ◽  
Vol 48 (2) ◽  
pp. 535-548
Author(s):  
Ewa FILIP ◽  
Aleksandra STROŃSKA ◽  
Magdalena SZENEJKO ◽  
Waldemar PLUTA
Keyword(s):  
Genetic Diversity ◽  
Common Wheat ◽  
Similarity Index ◽  
Principal Component ◽  
Shannon Index ◽  
Qualitative Assessment ◽  
Wheat Cultivars ◽  
Protein Markers ◽  
Binary Matrix ◽  
Sds Page

Knowledge of the composition subunits of proteins glutenins makes it much easier to determine the quality and suitability of the final product obtained from gluten. It is important to remember that not only molecular processes model gluten protein polymorphism. In this study, the genetic diversity of Polish common wheat cultivars was examined at the level of DNA and glutenin’s proteins HMW-GS. The SDS-PAGE and RAPD bands were evaluated in binary matrix, which was the basis for further analysis of results, using appropriate measures of variability: Q-Cochran  test (Cochran, 1950), p < 0.05; I-Shannon index; Si-Similarity index; PIC-Polymorphism Information Content; (PCA-Principal component analysis). Both types of markers proved to be useful in the overall assessment of genetic variability between tested of common wheat cultivars. The general genetic diversity indicates that good candidates with unique composition of HMW-GS subunits were selected among the examined cultivars and three OPA-02, OPA-03, OPB-08 primers with the highest power differentiating for the studied genotypes were selected. Results of the research revealed the potentials of RAPD and SDS-PAGE technique in determining genetical diversity and make a suitable qualitative assessment of common wheat cultivars.

scholarly journals Protein Profile Study of Some Nigerian Sesame (Sesamum indicum L.) Accessions

2015 ◽  
Vol 3 (2) ◽  
pp. 322-329 ◽  
Author(s):  
Gbenga Olorunshola Alege
Keyword(s):  
Genetic Diversity ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Sesamum Indicum ◽  
Genomic Changes ◽  
Sds Page ◽  
Sesame Genotypes ◽  
Species Specific ◽  
Total Seed

This study was carried out to investigate the genetic diversity among 23 sesame (Sesamum indicum L.) accessions obtained from different agro-ecological localities from 10 different states across 4 geopolitical zones in Nigeria using evidence from Sodium Dodecyl Polyacrylamide Gel Electrophoresis (SDS-PAGE). Total seed protein of the studied plants resolved on 12% SDS-PAGE showed variations in numbers and intensity of bands among the different sesame accessions. Thirteen (13) major bands were recorded in this study. Lack of unique band and presence of common band (band 7) among the 23 studied sesame accessions indicate some levels of genetic affinity and evidence of common evolutionary origin of the sesame genotypes. This band can therefore be tagged as species specific band for discriminating Sesamum indicum. Cluster analysis grouped the 23 sesame genotypes into two clusters with similarity coefficient ranging from 0.42 to 0.96 which indicates existence of genetic diversity; therefore there is ample opportunity for improving the 23 sesame genotypes. Variations in protein bands observed among the 23 studied plants could be attributed to genomic changes taken place during species diversification. It can be concluded that genetic diversity existed among Nigerian sesame for the improvement of characters of interest. Accessions 9 (YOL), 15(OTT), 22 (OFF) and 23 (JAL) are therefore recommended for used in future breeding programs for the development of improved sesame varieties.Int J Appl Sci Biotechnol, Vol 3(2): 322-329 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12734

scholarly journals Value of Seed Protein Profile in the Taxonomy of cultivars of Capsicum in Nigeria

2020 ◽  
Vol 36 (2) ◽  
pp. 1-8
Author(s):  
A.O. Adepoju ◽  
A.T.J. Ogunkunle ◽  
M.A. Azeez ◽  
A.G. Femi-Adepoju
Keyword(s):  
Seed Protein ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Taxonomic Status ◽  
Polymorphic Band ◽  
Dichotomous Key ◽  
Capsicum Spp ◽  
Total Seed ◽  
Genus Capsicum

Over a decade, the taxonomy of the genus Capsicum in Nigeria  has remained largely unrevised, unclassified and unidentified. As such, there is a dearth of information on the proper identification of Capsicum spp and relatives found in the country. The aim of this study was to re-examine the taxonomic status of the Capsicum in Nigeria in order to establish genetic diversity between them for proper identification and classification. Sodium dodecyl  polyacrylamide gel electrophoresis of total seed protein was performed on five varieties of Nigerian Capsicum spp., following standard procedures. Six protein bands were observed across the five cultivars of Capsicum, of which 12-14 Kda was the only polymorphic band. Only C. fructescens var. ijosi and C. fructescens var. sombo were unique for manifesting 20-24 and 15-16 Kda bands respectively. Dendrogram of analysis obtained resolved the taxa into two distinct groups. In the first group were cultivars of C. fructescens var. ijosi and sombo while in the second group were C. chinense, which was distinctly separated from C. fructescens var. bawa and C. annum. Artificial dichotomous key was constructed for the identification of members of the genus Capsicum available in Nigeria based on the protein profiles of their seeds. Keywords: Capsicum, seed protein, electrophoresis, identification, diversity.

scholarly journals Biochemical characterization based on SDS-page analysis and correlation among traits in opium poppy (Papaver somniferum L.) germplasm

Genetika ◽  
2015 ◽  
Vol 47 (3) ◽  
pp. 1029-1050 ◽  
Author(s):  
Nidhi Verma ◽  
Sudhir Shukla ◽  
Kusum Yadav ◽  
Brij Mishra ◽  
Anu Rastogi
Keyword(s):  
Protein Content ◽  
Seed Protein ◽  
Biochemical Characterization ◽  
Seed Storage Protein ◽  
Protein Profile ◽  
Similarity Index ◽  
Papaver Somniferum ◽  
Opium Poppy ◽  
Seed Protein Content ◽  
Variable Regions

This research aimed to investigate the genetic diversity based on the pattern of seed storage protein and to establish correlation between alkaloid and seed protein content among 124 germplasm lines of opium poppy (Papaver somniferum L.). Twenty seven polypeptide bands in range of 6 to 205 kDa were recorded. Similarity index was calculated by using Jaccard?s Similarity index and cluster analysis was performed using UPGMA model. Polymorphism was observed in three variable regions i.e., high, medium and low molecular weight, among which bands of 10.4, 20, 22, 24, 30, 32, 33, 49 and 205 kDa?s were common in all the germplasms but other bands showed variation. All the 124 germplasms were broadly grouped into 13 clusters based on average linkage method. None of the trait showed significant correlation with seed protein content. The differential protein profile of the germplasms revealed wide variability and diversity among the studied germplasms which could be further used in hybridization programme to obtain maximum gain.

scholarly journals Evaluation of Genetic Diversity of Seven Species of Solanum in Nigeria Using SDS-PAGE

2019 ◽  
pp. 1-6
Author(s):  
Busirat Odunayo Ogundairo ◽  
Muyiwa Segun Ayodele
Keyword(s):  
Genetic Diversity ◽  
Gel Electrophoresis ◽  
Seed Protein ◽  
Protein Profile ◽  
Generic Status ◽  
Molecular Weights ◽  
Sds Page ◽  
The Family ◽  
Intraspecific Relationships ◽  
Poly Acrylamide

Seed protein profile of 12 accessions belonging to 7 species of the genus Solanum of the family solanaceae were investigated through poly acrylamide gel electrophoresis. The accessions were selected based on their overlapping morphological associations observed on the field. Occurrence of two protein bands of molecular weights 100 kda and 20 kda in all accession showed interspecific relationships and could be used as generic bands among the genus Solanum. All morphologically similar accessions manifested similarities in their protein bands except S. melongena. Dendrogram based on upgma also revealed the generic status and intraspecific relationships of the accession of S. aethiopicum and S. macrocarpon.

scholarly journals Keragaman Genetik Bawang Merah (Allium cepa L.) Berdasarkan Marka Morfologi dan ISSR

2017 ◽  
Vol 45 (2) ◽  
pp. 175
Author(s):  
Vebrita Sari ◽  
Miftahudin , ◽  
Dan Sobir
Keyword(s):  
Genetic Diversity ◽  
Genetic Resource ◽  
Geographic Origin ◽  
Morphological Characters ◽  
Issr Markers ◽  
Breeding Program ◽  
Polymorphic Band ◽  
Morphological Markers ◽  
Allium Cepa L ◽  
Issr Primers

Development of new shallot varieties requires genetic variation of the germplasm. However, information on genetic diversity of local shallot in Indonesia is still lacking. This study was aimed to analyze the genetic diversity of shallot based on morphological characters and ISSR markers for management of genetic resource and breeding program in Indonesia. This study was conducted during October 2014 to September 2015. The 34-shallot genotypes were collected from several regions in Indonesia in the form of the bulb. Twenty four morphological markers and 13 ISSR primers were used in this research. The informative characters to analyze genetic diversity of shallot were foliage cracking, bulb number and bulb morphological characters. ISSR markers generated 103 DNA polymorphic band with the total of 89.57% and the informative primers were ISSRred 4, ISSRred 9 and ISSRred 20. Both morphological and ISSR markers showed 27% genetic diversity and grouped all genotypes into two main groups; however the grouping did not relate to the geographic origin. The largest bulb diameter of 7.54 cm was obtained from Bangkok variety and the highest number of bulb per clump of 27 bulb/clump was produced by the genotype from Pekanbaru. These genotypes can be used as potential parents for shallot breeding program in Indonesia. <br /><br />Keywords: genetic diversity, ISSR markers, morphological characters, shallot<br /><br />

scholarly journals Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesian archipelago based on morphological and inter-simple sequence repeats markers

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Kurnia Makmur ◽  
TATIK CHIKMAWATI ◽  
SOBIR SOBIR
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Similarity Index ◽  
Morphological Characters ◽  
Molecular Data ◽  
Issr Markers ◽  
Morphological Observation ◽  
Indonesian Archipelago ◽  
Morphological And Molecular Data ◽  
Issr Primers

Abstract. Makmur K, Chikmawati T, Sobir. 2020. Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesia based on morphological characters and ISSR markers. Biodiversitas 21: 5948-5953. Lemon basil (Ocimum × africanum Lour.) or kemangi is a highly beneficial plant, yet still underutilized. Therefore, providing necessary information on genetic diversity of this species is essential for further utilization. The aim of this study was to elucidate genetic diversity of 33 accessions of O. × africanum collected from four Islands of Indonesia along with three accessions of Ocimum basilicum L. Morphological observation was conducted on 37 morphological characters following the International Union for the Protection of New Varieties of Plants descriptor guidelines; subsequently, 13 ISSR primers were employed in molecular analysis. Both morphological and molecular data were analyzed based on simple matching similarity index using UPGMA method. Morphologically, O. × africanum and O. basilicum were clearly separated at the similarity index of 0.52, and among two species were divided into two groups according to two either character. ISSR analysis using 13 ISSR primers produced 111 DNA bands, and 108 of them (97.29%) were polymorphic. Cluster analysis based on ISSR data could not explicitly separate O. × africanum and O. basilicum accessions. Besides, Ocimum accessions collected from the same area did not always cluster into one group.

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