In Vitro Direct Regeneration through Cotyledon Culture in Pigeon Pea [Cajanus Cajan (L.) Millsp.] and Evaluation of Genetic Fidelity using RAPD Markers

2017 ◽  
Vol 30 (1) ◽  
pp. 4
Author(s):  
Mahitha Banala ◽  
Rajinikanth Marka ◽  
Archana Pamulaparthi ◽  
Rama Swamy Nanna
Keyword(s):  
Cajanus Cajan ◽  
Rapd Markers ◽  
Genetic Fidelity ◽  
Pigeon Pea ◽  
Direct Regeneration ◽  
Cotyledon Culture

Establishment of genetic fidelity of In vitro-raised Lilium bulblets through rapd markers

2001 ◽  
Vol 37 (2) ◽  
pp. 227-231 ◽  
Author(s):  
Anushri Varshney ◽  
M. Lakshmikumaran ◽  
P. S. Srivastava ◽  
Vibha Dhawan
Keyword(s):  
Rapd Markers ◽  
Genetic Fidelity

scholarly journals FORMULATION AND EVALUATION OF ORODISPERSIBLE TABLETS (ODTS) OF DICLOFENAC SODIUM BY USING SUPERDISINTEGRANT FROM NATURAL ORIGIN

2019 ◽  
pp. 190-197
Author(s):  
SATYAJITH PANDA ◽  
NODAGALA HEMALATHA ◽  
PANCHAGNULA UDAYA SHANKAR ◽  
SRINIVASA RAO BARATAM
Keyword(s):  
Cajanus Cajan ◽  
Diclofenac Sodium ◽  
Pigeon Pea ◽  
Disintegration Time ◽  
In Vitro Drug Release ◽  
Sodium Starch Glycolate ◽  
Orodispersible Tablets ◽  
Pregelatinized Starch ◽  
The Stability

Objective: In this study, a polysaccharide isolated from the seeds of Cajanus cajan (pigeon pea) was investigated as a super disintegrant in the orodispersible tablets of diclofenac sodium. Methods: Diclofenac sodium tablets were prepared separately using different concentrations (5%, 7.5%, 10%, and 15% w/w) of isolated Cajanus cajan seed polysaccharide (natural) and sodium starch glycolate (synthetic) as super disintegrant by the direct compression method. Evaluation of tablets was done for various pre-and post-compression parameters. The stability studies were performed on optimized formulation F5. The disintegration time and in vitro drug release of the formulation F5 was compared with pregelatinized starch and synthetic super disintegrant (sodium starch glycolate). Results: The drug-excipient interactions were characterized by Fourier transform infrared studies. The Optimized formulation F5 containing 15% polysaccharide showed wetting time of 118.7 seconds with 105.3 seconds of disintegration time and 95.61% dissolved in 3 min. Conclusion: The present work revealed that Cajanus cajan seed polysaccharide has a good disintegrating agent in the formulation of orodispersible tablets.

scholarly journals DEVELOPMENT AND CHARACTERIZATION OF ORO-DISPERSIBLE TABLETS OF METFORMIN HYDROCHLORIDE USING CAJANUS CAJAN STARCH AS A NATURAL SUPERDISINTEGRANT

2022 ◽  
pp. 139-147
Author(s):  
SONIA DHIMAN ◽  
RITCHU BABBAR ◽  
THAKUR GURJEET SINGH ◽  
SHIVANGI ANAND ◽  
ASHI MANNAN ◽  
...  
Keyword(s):  
Cajanus Cajan ◽  
Research Work ◽  
Pigeon Pea ◽  
Granular Structure ◽  
Metformin Hydrochloride ◽  
Compression Process ◽  
Disintegration Time ◽  
Orodispersible Tablets ◽  
Dispersible Tablets

Objective: The aim of the research work was to explore the use of Cajanus cajan (Pigeon pea) polysaccharide as a superdisintegrant. The novel superdisintegrant has been evaluated for its action by incorporating it into orodispersible tablets of Metformin Hydrochloride. Methods: Cajanus cajan starch was extracted from its seeds and superdisintegrant was developed by microwave modification of the extract. Various characterization tests such as gelatinization temperature, water absorption index, pH, and viscosity were used to identify the microwave-modified polysaccharide. The orodispersible tablets were made using a direct compression process employing varying concentrations of modified Cajanus cajan starch. Prepared tablets were tested for several pre and post-compression parameters and compared with a well-established synthetic superdisintegrant, sodium starch glycolate. The stability studies were conducted on an optimized formulation. Results: Fourier transform infrared spectroscopy study showed that the drug had no interactions with the microwave-modified Cajanus cajan starch. SEM confirmed that Cajanus cajan starch granules exhibited intact granular structure in oval shapes and smooth surfaces. After microwave modification, the Cajanus cajan starch component lost its granular structure, which further led to the generation of surface pores and internal channels, causing overall swelling responsible for superdisintegrant activity. The optimized formulation (ODF5) containing 15 % modified Cajanus cajan starch performed better in terms of wetting time (22.21 s), disintegration time (53.3 s), and in vitro drug release (92%), as compared to formulation prepared by synthetic superdisintegrant (ODF1). Conclusion: The present investigation concluded that modified Cajanus cajan starch has good potential as a superdisintegrant for formulating oro-dispersible tablets. Furthermore, modified Cajanus cajan starch is inexpensive, non-toxic and compatible in comparison with available synthetic superdisintegrants.

scholarly journals Kajian Aktivitas Antikanker Ekstrak Daun Gude (cajanus cajan) Terhadap Sel Kanker Kolon Secara in Vitro

2017 ◽  
Vol 6 (1) ◽  
pp. 31
Author(s):  
Muji Rahayu ◽  
Roosmarinto Roosmarinto
Keyword(s):  
Colorectal Cancer ◽  
Cajanus Cajan ◽  
Methanol Extract ◽  
Cancer Therapeutics ◽  
Pigeon Pea ◽  
Biochanin A ◽  
Cytotoxicity Test ◽  
Diphenyltetrazolium Bromide ◽  
Colorectal Cancer Cells

Colorectal cancer is one of the five type of highest insiden in Indonesia. One of development cancer therapeutics directed to combination of chemotherapeutic agent and chemopreventive compounds. One approach to finding a chemopreventive compounds is through the exploration of natural ingredient especialy herbs. Flavonoids is group of compounds from plants that have been widely studied as anti-cancer activity. Leave of pigeonpea (Cajanus cajan) is, kind of Legumes plants rich in flavonoids among others cajanol, quercetin, luteolin, apigenin formonentin, vitexin, orientin, biochanin A, pinostrobin, dan isorhamnetin. This study aims to determine the content of chemical compounds in the methanol extract of leave pigeon pea that has anticancer activity using WiDr colorectal cancer cells with MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) method, followed by flowcytometry test. The result of the cytotoxicity test of methanol extract of pigeonpea leaves have IC50 of 307 ug/ml and with flowcytometry method showed necrosis WiDr cell.

scholarly journals Evaluation of Anticonvulsant Activity of Ethanolic Extract of Leaves of Cajanus cajan (L) Millsp. In Rodents

2019 ◽  
Vol 11 (06) ◽  
Author(s):  
Ankita Prakash Kore ◽  
Nilofar Shahnwaj Naikwade ◽  
Padma Laxmikant Ladda ◽  
Tabbasum Shabbirahmad Shikalgar ◽  
Shirish Sidgonda Patil
Keyword(s):  
Cajanus Cajan ◽  
Anticonvulsant Activity ◽  
Ethanolic Extract ◽  
Pigeon Pea ◽  
Anticonvulsant Effect ◽  
Dependent Manner ◽  
Standard Drug ◽  
Phytochemical Study ◽  
Perennial Plant

Cajanus cajan (L) Millsp. is the perennial plant belongs to family Fabaceae, commonly called as Pigeon pea plant. The presence of phytoconstituents like flavonoids, the flavanone (substituted) from Cajanus cajan (L) Millsp. have in vitro neuroactive property. This flavanone named as pinostrobin helps to inhibit voltage – gated sodium channels. Because of its bioactive phytoconstituents it may act as antiepileptic drug. To avoid problems like ADR herbal plant might be alternative to treat epilepsy. The current study was therefore carried out to evaluate antiepileptic activity of Ethanolic extract of leaves of Cajanus cajan in rodents. The effect of ELECC in MES-induced convulsions in rat and PTZ-induced convulsion in mice was evaluated using doses 100 mg/kg and 200 mg/kg for 7 days. Phenytoin (25 mg/kg), Diazepam (4 mg/kg) was used as standard drug for respective model. Depending on the model, outcome measures were abolishment of Hind Limb Tonic Extensor phase in MES-induced convulsion in rat and onset of latency, recovery or death in PTZ-induced convulsion in mice as well as biochemical estimation of amino acid neurotransmitter (GABA, Glutamate) were evaluated. The ELECC at doses 100 and 200 mg/kg significantly delayed the HLTE phase in MES-induced convulsions in rat whereas, significantly increased onset of latency in PTZ-induced convulsion in mice. It also showed significant (p>0.0001) effect on the level of GABA and Glutamate in dose dependent manner in both models. The phytochemical study of C. cajan showed the presence of Glycosides, Flavonoids, Flavonones, Steroids, Tannins, Fixed oil, Fatty acids and Proteins. As the flavonoids present in C. cajan may contribute to the anticonvulsant activity of the plant. Therefore, the presence of such compounds in the extract may be responsible for the anticonvulsant effect. Therefore, present study validates its anticonvulsant activity. Further, research is required to elucidate its specific mechanism of action and isolation of responsible active principles.

scholarly journals Biomass yield and fodder quality of Napier grass (Pennisetum purpureum) as affected by Pigeon pea (Cajanus cajan) intercrop and planting distance

2019 ◽  
Vol 54 (2) ◽  
pp. 36-44
Author(s):  
E. A. Tenakwa ◽  
S. Cudjoe ◽  
T. Ansah
Keyword(s):  
Cajanus Cajan ◽  
Biomass Yield ◽  
Pigeon Pea ◽  
Napier Grass ◽  
Pennisetum Purpureum ◽  
Fodder Quality ◽  
Significant Difference ◽  
Planting Distance

The study sought to investigate the effect of two planting distances and Pigeon Pea (Cajanus cajan) intercrop on the biomass yield, chemical composition and in vitro organic matter digestibility (IVOMD) of Napier grass (Pennisetum purpureum). The treatments were laid in a 2x2 factorial RCBD. The factors were two planting distances (0.5x0.5 and 0.5x1m) and two levels of intercrop (Pigeon Pea intercrop and sole Napier grass). At 90-days, the grasses were harvested and biomass yield measured. The fodder was separated into leaves and stems for chemical and digestibility analysis in 2x2x2 factorial in CRD. The interaction at all levels was not significant for all the parameters. There was a significant main effect of planting distance, intercrop and botanical fraction on biomass yield, plant height, tiller number and fodder quality. Whilst biomass yield was higher (P=0.02) in the 0.5x0.5m, no significant difference was recorded in the effect of the intercrop. Whilst intercrop affected (P<0.05) IVOMD and ME, botanical fraction significantly affected DM, CP and ME. The leaf fraction had more (P<0.05) DM, CP and ME than the stem fraction. The study revealed that close planting distance enhanced biomass yield whilst Pigeon Pea intercrop and botanical fraction affected fodder quality of Napier grass.

scholarly journals Exploration of Rhizobacteria as Bioagents against Phytophthora Blight and Yield Attributes of Pigeonpea (Cajanus cajan L.): In vitro and In vivo Study

2021 ◽  
pp. 25-33
Author(s):  
Saroj Bala ◽  
Rajni Devi ◽  
Veena Khanna
Keyword(s):  
Plant Growth ◽  
Cajanus Cajan ◽  
Biochemical Characterization ◽  
Growth Parameters ◽  
Pigeon Pea ◽  
Yield Attributes ◽  
Plant Growth Promoting ◽  
Growth Promoting

The latest soil management scenario is occupied by destructive chemical fertilizers, which is a serious risk to both human health as well as to the environment. Advantageous microbes present in soil are used as a biofertilizers for a promising role in sustainable agriculture. Pigeon pea (Cajanus cajan L.) is a primitive protein rich leguminous pulse in India. Thirty-five isolates from rhizospheric soil samples were collected from twelve different locations of Punjab (India). Morphological and biochemical characterization for selection of potential plant growth promoting traits with antifungal properties was undertaken. Most of the inoculated seeds with rhizoisolates evolved a significant increase in growth parameters of pigeon pea as compared to uninoculated seeds, both in vitro and in vivo conditions. Plant growth promoting rhizobacterias (PGPRs) are environmentally safe as they lead to increased production and resistance against diseases of crops.

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