scholarly journals Dynamics of growth parameters of Gentiana lutea L. in vitro plants under different lighting conditions

2021 ◽  
Vol 28 ◽  
pp. 58-65
Author(s):  
L. R. Hrytsak ◽  
M. Z. Prokopiak ◽  
O. Yu. Mayorova ◽  
Kh. M. Kolisnyk ◽  
N. M. Drobyk
Keyword(s):  
Growth Parameters ◽  
Spectral Composition ◽  
Light Flux ◽  
In Vitro Plants ◽  
Gentiana Lutea ◽  
Plant Cultivation ◽  
In Situ Conditions ◽  
Flux Intensity ◽  
Cultivation In Vitro

Aim. Study of the dynamics Gentiana lutea L. plant growth processes in vitro depending on the light regime changes of their cultivation in order to develop a scheme to increase their adaptive potential. Methods. Methods of plant cultivation in vitro, biometric method, as well as ANOVA variance analysis and middle group analysis in pairs using the Tukey test (Tukey test) were used. Results. It is shown that the cultivation of G. lutea plants in vitro using 25 W/m2 light flux intensity in the region of photosynthetically active radiation and the ratio of blue (Eb): green (Eg): red (Er) ranges = 41.8%: 42.7 %: 15.5% triggers non-specific photomorphogenesis reactions for intact plants, which lead to poor root system development, stem elongation, formation of small leaves with a thin leaf blade, overall low productivity and low adaptation potential of G. lutea plants to ex vitro and in situ conditions. Increasing the light flux intensity to 44 W/m2 and increasing the red wave proportion up to 20.3% allows not only to improve the bioproductivity of G. lutea plants which are cultivated in vitro, but also to increase the coefficient of microclonal reproduction without the additional use of exogenous growth regulators. The greatest growth of the aboveground and underground parts, increase in effective leaf surface are observed in vitro plants during cultivation at 135 W/m2 light flux intensity and spectral composition Eb: Eg: Er = 29.5%: 32.5%: 38.0%. Conclusions. It is possible to improve plant bioproductivity by changing the light conditions of plant cultivation in vitro, and, accordingly, to increase the adaptive potential to ex vitro and in situ conditions. Keywords: Gentiana lutea L., in vitro plants, light flux intensity, spectral composition, growth parameters.

scholarly journals Modern technologies of increasing the tolerance of in vitro cultured plants to ex vitro conditions

2020 ◽  
Vol 26 ◽  
pp. 183-189
Author(s):  
L. R. Hrytsak ◽  
N. M. Drobyk
Keyword(s):  
Ex Vitro ◽  
Adaptive Potential ◽  
In Vitro Plants ◽  
Plant Cultivation ◽  
Planting Material ◽  
Chemical Conditions ◽  
Cultivation In Vitro ◽  
Physical And Chemical ◽  
Modern Technologies

Aim. To analyze the experience of Ukrainian and foreign scientists on technologies to increase the adaptive potential of cultivated in vitro plants to ex vitro conditions. Results. Modern acclimatization technologies are mainly aimed at improving the methods of adaptation of planting material of in vitro collections to ex vitro conditions. Much less attention is paid to technologies to increase plant resilience at the stage of their multiplication and growth in vitro. Integration and systematization of research results of a large number of scientists is allowed to describe the main strategies and methodological techniques, which implementation can significantly increase the adaptive potential of in vitro plants. Conclusions. Optimization of physical and chemical conditions of plant cultivation in vitro can induce changes in their phenotype, intensity of photosynthetic reactions, water balance, which increases the adaptive potential of plants and facilitates the process of their acclimatization to ex vitro conditions. Key words: in vitro plants, acclimatization to ex vitro conditions, adaptive potential, technology.

scholarly journals The influence of lighting spectral composition on physiological response of potato in vitro

2021 ◽  
pp. 8-11
Author(s):  
Alexandra Mikhailovna Varushkina ◽  
Azalia Ildarovna Yakhina ◽  
Alisa Sergeevna Shirinkina ◽  
Lybov Gennadevna Tsoma ◽  
Anna Leonidovna Latypova
Keyword(s):  
Physiological Response ◽  
Growth Parameters ◽  
Light Emitting Diode ◽  
Spectral Composition ◽  
Specific Response ◽  
Light Sources ◽  
Time Data ◽  
Potato Varieties ◽  
Potato Plants

The microclonal (in vitro) propagation of the original potato plants is important stage in the cultivation of high-quality seed potato. The appropriate microclimate parameters (illumination, temperature, air humidity) are to be maintained in phytotrons for optimal clones’ growth. It is necessary to consider the specific response of potato varieties and, according to it, carefully select the lighting spectral composition. The purpose of the work was to study the effect of various light sources on the morphological parameters of 22 varieties of potato microplants. 10 clones of every variety were selected for the experiment after the initial multi-varietal analysis. After 27 days of cultivation, growth parameters were measured: root length, plant height and the number of internodes. The light sources were light-emitting diode (LED) irradiators ECOLED-BIO-195W-D120 FMatrix IP66, ECOLED-BIO-112-185W-D120 UniversalLED, and 3-ECOLED-60-LX Fito IP65 (“SET” ltd). For the first time, data on the lighting effect on a large sample of potato varieties were obtained, demonstrating significant differences in the physiological response of plants to lighting conditions. According to the research results, it was shown that ECOLED-60-LX Fito IP65 was the optimal source of photosynthetically active radiation for plants among the studied LED-lamps, providing a positive effect on the growth parameters of potato plants, including plants height, length of roots and internodes amount.

scholarly journals Effect of Beta-Lactam Antibiotics on Microspore Embryogenesis in Brassica Species

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 489
Author(s):  
Anna Mineykina ◽  
Daria Shumilina ◽  
Ludmila Bondareva ◽  
Alexey Soldatenko ◽  
Elena Domblides
Keyword(s):  
Microspore Embryogenesis ◽  
Brassica Species ◽  
Cultivated Plants ◽  
Beta Lactam ◽  
Negative Effects ◽  
Beta Lactam Antibiotics ◽  
Plant Cultivation ◽  
Embryo Yield ◽  
Cultivation In Vitro

Antibiotics are widely applied for plant cultivation in vitro to eliminate bacterial contamination. However, they can have both positive and negative effects on the cells of cultivated plants, and these effects largely depend on the type antibiotic used and its concentration. The objective of the present study was to estimate the effect of β-lactam antibiotics ampicillin (Amp) and cefotaxime (Cef) on microspore embryogenesis induction in vitro in the Brassica species. The performed experiments confirmed cefotaxime inhibits microspores in B. napus and B. oleracea, even in concentrations as low as 50 mg/L. The highest embryo yield was obtained for B. napus in the NLN-13 medium with added ampicillin in concentrations of 50–100 mg/L as an antimicrobial agent. This embryo yield was significantly higher than that obtained in a medium without supplemented antibiotics and two times higher than in the medium with added cefotaxime. Analogous results were obtained for B. oleracea and B. rapa.

scholarly journals The effect of led light sources with varied spectral composition on the in vitro rooting bility of garden strawberry (Fragaria × ananassa)

2019 ◽  
Vol 180 (1) ◽  
pp. 33-37 ◽  
Author(s):  
I. A. Byadovsky
Keyword(s):  
Red Light ◽  
Spectral Composition ◽  
Light Flux ◽  
Light Sources ◽  
Fragaria Ananassa ◽  
Fluorescent Lamp ◽  
Led Light ◽  
Rooting Ability ◽  
Strawberry Cultivars

Consideration is given to the possibility of using LED light sources with different ratios of red (650 - 660 nm), orange (600 - 610 nm) and blue (440 - 450 nm) light in the light flux in the rooting stage of garden strawberry (Fragaria×ananassa(Duch. ex Weston) Duch. ex Rozier) grownin vitro. The study covered a sizable diversity of strawberry cultivars (selected from thein vitrocollection): ‘Borovitskaya’, ‘Vityaz’, ‘Zenga Zengana’, ‘Khoney’, ‘Tsaritsa’, ‘Kubata’, ‘Dukat’, ‘Marmalada’, ‘Valenta’, ‘Rusich’, ‘Krasavitsa Zagorya’, ‘Neznakomka’ and ‘Roksana’, thus ensuring quite diversified results. It was established that the use of LED sources with an increased content of red light in the light flux increased the rooting percentage (1.15-1.25 times) and the number of roots per one microcutting (1.29-1.77 times) inin vitroculture, as compared with fluorescent lamp lighting. It was observed that with different spectral compositions of light the rooting ability of microcuttings was cultivar-specific.

scholarly journals The influence of light regime on the growth data and pigment composition of the plant Gentiana lutea cultured in vitro

2018 ◽  
Vol 9 (2) ◽  
pp. 258-266
Author(s):  
L. R. Hrytsak ◽  
A. I. Herts ◽  
N. V. Nuzhyna ◽  
M. M. Cryk ◽  
V. V. Shevchenko ◽  
...  
Keyword(s):  
Light Regime ◽  
Ex Vitro ◽  
Pigment Composition ◽  
Growth Data ◽  
Gentiana Lutea ◽  
Fluorescent Lamps ◽  
In Situ Conditions ◽  
Planting Materials

New technologies of reintroduction of plant species presuppose implementing both traditional and biotechnological methods for obtaining certain planting materials. However, plants cultivated in vitro exist in specific conditions that lead to changes in their structural and functional state. This explains why it is hard for them to adapt to ex vitro and in situ conditions. Therefore, there is a need for the development of a multistage method of cultivating in vitro plants that would make the influence on their adaptive mechanism in ex vitro and in situ conditions possible. One of its stages is the optimization of the light regime of cultivation which can both initiate the change of the state of the photosynthetic apparatus of plants and increase their bioproductivity stimulating the work of their protective system. This work studies changes in the morphogenesis, growth data and pigment composition of the rare species of Gentiana lutea L. of three populations in the Ukrainian Carpathian (mountains Pozhyzhevska and Sheshul-Pavlyk, plateau Lemska) in vitro focusing particularly on the cultivation light regime. The research has proved the inefficiency of using fluorescent lamps of daylight lamps (LD) type as source of illumination because the low intensity of luminous flux in the area of photosynthetically active radiation (PAR), as well as high proportion of wavelength of blue (400–500 nm) and green (500–600 nm) range in the spectrum cause specific reactions of photomorphogenesis, which, despite the high content of pigments in plastids, lead to poor development of root systems, stretching the stems, formation of small leaves with thin leaflet plate, generally low productivity and low adaptive potential of G. lutea plants to ex vitro and in situ conditions. Complement of cold white light lamps to the fluorescent lamps LD type in the ratio of 1 : 1 enables one to increase the intensity of illumination in the field of PAR and raise the fraction of wavelength of red range (600–700 nm). Such light conditions both improve the bio-productivity of G. lutea plants of all three populations cultured in vitro in comparison to the LD type regimen, reducing the content of chlorophyll b and carotenoids in light-harvesting complexes of photosystems and facilitate an increase in the microclonal multiplication factor without using higher concentrations of exogenous growth regulators,which significantly reduces the cost of the process of obtaining planting materials. It was proved that a combination of LD type lamps, cold white light lamps and phytolamps in the ratio 1 : 1 : 0.6 should be used on the final stages of preparation of the planting material of G. lutea before transferring it to ex vitro and in situ conditions. This relates to the fact that the increase of the wavelength of the red range results in the widening of the active surface of the leaves, rise in the content of photosynthetic pigments, and the noticeable growth of the aboveground and underground parts of the plants. The article assumes that the use of such illumination mode will ensure a faster transition of cultured in vitro G. lutea plants from heterotrophic to autotrophic nutrition, improving their adaptive potential and enabling easier adaptation to non-sterile ex vitro and in situ conditions.

Influence of salicylic acid on organogenesis of in vitro plants Fragaria ananassa Duch.

2016 ◽  
Vol 48 (1) ◽  
pp. 26-33
Author(s):  
O.V. Subin ◽  
◽  
M.D. Melnychuk ◽  
A.F. Likhanov ◽  
O.L. Klyachenko ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Fragaria Ananassa ◽  
In Vitro Plants

scholarly journals In vitro Osmotic Stress Memory in Chrisanthemum hybridum: Structural and Physiological Responses

2017 ◽  
Vol 27 (2) ◽  
pp. 161-169 ◽  
Author(s):  
Lidiia Samarina ◽  
Valentina Malyarovskaya ◽  
Yulija Abilfazova ◽  
Natalia Platonova ◽  
Kristina Klemeshova ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Relative Water Content ◽  
Soluble Protein ◽  
Growth Parameters ◽  
Physiological Responses ◽  
Proline Content ◽  
Stress Exposure ◽  
Stress Memory ◽  
Relative Water

Structural and physiological responses of chrysanthemum to repeated osmotic stress were studied. Plants were cultured for 2 weeks (for each stress1 and stress 2) on half MS supplemented with mannitol 100 mM (Treatment I) and 200 mM (Treatment II). First stress inhibited growth parameters stronger than second stress in treatment I. In treatment II both stress events strongly inhibited growth parameters of micro‐shoots. Proline content exceeded control 6 ‐ 8 times after 1st stress, and 2 ‐ 5 times after the 2nd stress in treatments I and II, respectively. Soluble protein was accumulated in leaves during both stress exposures, and 2 ‐ 2.5 times exceeded control after the 2nd stress. Relative water content in both treatments increased after the 2nd stress exposure. In treatment II chlorophyll а and carotenoids contents were 8.78 and 4.62 mg/g comparing to control (4.21 and 2.25 mg/g, respectively) after the 1st stress. But after the 2nd stress there was no difference with control.Plant Tissue Cult. & Biotech. 27(2): 161-169, 2017 (December)

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