scholarly journals The influence of lighting spectral composition on physiological response of potato in vitro

2021 ◽  
pp. 8-11
Author(s):  
Alexandra Mikhailovna Varushkina ◽  
Azalia Ildarovna Yakhina ◽  
Alisa Sergeevna Shirinkina ◽  
Lybov Gennadevna Tsoma ◽  
Anna Leonidovna Latypova
Keyword(s):  
Physiological Response ◽  
Growth Parameters ◽  
Light Emitting Diode ◽  
Spectral Composition ◽  
Specific Response ◽  
Light Sources ◽  
Time Data ◽  
Potato Varieties ◽  
Potato Plants

The microclonal (in vitro) propagation of the original potato plants is important stage in the cultivation of high-quality seed potato. The appropriate microclimate parameters (illumination, temperature, air humidity) are to be maintained in phytotrons for optimal clones’ growth. It is necessary to consider the specific response of potato varieties and, according to it, carefully select the lighting spectral composition. The purpose of the work was to study the effect of various light sources on the morphological parameters of 22 varieties of potato microplants. 10 clones of every variety were selected for the experiment after the initial multi-varietal analysis. After 27 days of cultivation, growth parameters were measured: root length, plant height and the number of internodes. The light sources were light-emitting diode (LED) irradiators ECOLED-BIO-195W-D120 FMatrix IP66, ECOLED-BIO-112-185W-D120 UniversalLED, and 3-ECOLED-60-LX Fito IP65 (“SET” ltd). For the first time, data on the lighting effect on a large sample of potato varieties were obtained, demonstrating significant differences in the physiological response of plants to lighting conditions. According to the research results, it was shown that ECOLED-60-LX Fito IP65 was the optimal source of photosynthetically active radiation for plants among the studied LED-lamps, providing a positive effect on the growth parameters of potato plants, including plants height, length of roots and internodes amount.

scholarly journals IN VITRO DEVELOPMENT OF YELLOW LAPACHO (BIGNONIACEAE) USING HIGH-POWER LIGHT EMITTING DIODE

2018 ◽  
Vol 42 (5) ◽  
Author(s):  
Ezequiel Enrique Larraburu ◽  
Gonzalo Sanchez Correa ◽  
Berta Elizabet Llorente
Keyword(s):  
In Vitro Culture ◽  
High Power ◽  
Stomatal Density ◽  
Light Emitting Diode ◽  
Dry Weight ◽  
Light Sources ◽  
Multiplication Rate ◽  
Light Emitting ◽  
Shoot Dry Weight

ABSTRACT Handroanthus ochraceus (yellow lapacho) is a medicinal, ornamental and timber tree which can be propagated by in vitro culture. Conventional methods use fluorescent lighting (FL), whereas light emitting diode (LED) has been used for this purpose only recently. The aim of this work was to evaluate the effects of FL and high-power LED (HP-LED) on the in vitro multiplication and rooting of yellow lapacho at different irradiances (15 to 60 µmol m-2s-1). Epicotyls obtained from half-siblings was multiplicated in WPM (Woody Plant Medium) supplemented with 20 µM benzilaminopurine and 1 mM IBA (indolebutiric acid). For rooting, shoots were cultured for 3 days in ½WPM supplemented with 50 µM IBA and for 42 days in auxin-free ½WPM under HP-LED or FL lighting. Under HP-LED, the multiplication rate of shoots increased significantly (61%) from 20 to 40 µmol m-2s-1 respect to FL. Differences in abaxial stomatal density and size were observed between light sources at 20 µmol m-2s-1. High HP-LED irradiance produced the highest rooting percentage. In the rooting stage, the marginal means of treatments without factors interaction showed that HP-LED irradiances significantly increased shoot length by 20%, shoot fresh weight by 77% and shoot dry weight by 30% in comparison to the values under FL. The maximum values calculated from the regression curves were around 50 µmol m-2 s-1 for HP-LED for all parameters except root lenght whereas were around 20 µmol m-2 s-1 for FL for all parameters except fresh and dry weigth of shoot. Here we show that HP-LED lighting improve in vitro culture of H. ochraceus, reduced 81% energy consumption respect to FL and uses only a multispectral LED instead of different single color LEDs. Therefore, HP-LED could be useful for the micropropagation of tree species contributing to sustainable agriculture and ecological restoration of degraded areas.

scholarly journals Morphogenetic and physiological effects of LED spectra on the apical buds of Ficus carica var. Black Jack

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ankita Rajendra Parab ◽  
Kho Ying Han ◽  
Bee Lynn Chew ◽  
Sreeramanan Subramaniam
Keyword(s):  
Callus Formation ◽  
Light Emitting Diode ◽  
Multiple Shoots ◽  
Cellular Level ◽  
Ficus Carica ◽  
Light Sources ◽  
Light Emitting ◽  
Apical Buds ◽  
Red Led

AbstractThe use of artificial light sources such as light-emitting diodes (LEDs) has become a prerequisite in tissue culture studies to obtain morphogenetic enhancements on in vitro plants. This technology is essential for developmental enhancements in the growing plant cultures due to its light quality and intensity greatly influencing the in vitro growing explants at a cellular level. The current study investigates the effects of different light-emitting diode (LED) spectra on the growth of apical buds of Ficus carica var. Black Jack. Ficus carica, commonly known as figs is rich in vitamins, minerals, and phytochemicals capable of treating microbial infections and gastric, inflammatory, and cardiac disorders. Apical buds of Ficus carica var. Black Jack, presented morphogenetic changes when grown under six different LED spectra. The highest multiple shoots (1.80 per growing explant) and healthy growing cultures were observed under the blue + red LED spectrum. Wound-induced callus formation was observed on apical buds grown under green LED spectrum and discolouration of the growing shoots were observed on the cultures grown under far-red LED spectrum. Multiple shoots obtained from the blue + red LED treatment were rooted using 8 µM indole-3-acetic acid (IAA), and the rooted plantlets were successfully acclimatised. Compared with the other monochromatic LEDs, blue + red proved to be significantly better for producing excellent plant morphogeny. It is apparent that blue and red LED is the most suitable spectra for the healthy development of plants. The findings have confirmed that the combination of blue + red LED can potentially be used for enhancing growth yields of medicinally and commercially important plants.

scholarly journals Effects of Different LED Light Recipes and NPK Fertilizers on Basil Cultivation for Automated and Integrated Horticulture Methods

2021 ◽  
Vol 11 (6) ◽  
pp. 2497
Author(s):  
Silvia Barbi ◽  
Francesco Barbieri ◽  
Alessandro Bertacchini ◽  
Luisa Barbieri ◽  
Monia Montorsi
Keyword(s):  
Controlled Release ◽  
Growth Parameters ◽  
Light Emitting Diode ◽  
Substantial Improvement ◽  
Fresh Mass ◽  
Light Sources ◽  
Npk Fertilizers ◽  
Led Light ◽  
Deep Blue ◽  
Led Lights

This study aims to optimize the conditions for “Genovese” basil (Ocimum Basilicum) germination and growth in an indoor environment suitable for horticulture through a synergic effect of light and fertilizers addition. In fact, several studies determined that specific light conditions are capable of enhancing basil growth, but this effect is highly dependent on the environmental conditions. In this study, the effect of different light sources was determined employing a soil with a negligible amount of fertilizer, demonstrating substantial improvement when light-emitting diode (LED) lights (hyper red and deep blue in different combinations) were applied with respect to daylight (Plants height: +30%, Total fresh mass: +50%). Thereafter, a design of experiment approach has been implemented to calculate the specific combination of LED lights and fertilizer useful to optimize the basil growth. A controlled-release fertilizer based on nitrogen, phosphorus, and potassium (NPK) derived from agro-residues was compared with a soil enriched in macronutrients. The results demonstrate significant improvements for the growth parameters with the employment of the controlled-release NPK with respect to enriched soil combined with a ratio of hyper red and deep blue LED light equal to 1:3 (Total fresh mass: +100%, Leaves number: +20%).

PROSPECTIVE POTATO VARIETIES’ RECOVERY IN VITRO

2020 ◽  
pp. 467-468
Author(s):  
L.N. Fyodorova
Keyword(s):  
Effective Concentration ◽  
Potato Varieties ◽  
Potato Plants ◽  
Viral Preparation

As research shows, the most effective concentration of the Aciclovir anti-viral preparation is the dose of 15 mg/l, which definitely removes potato plants of the M. virus.

Responses of Favorita Potato Plantlets Cultured in Vitro under Fluorescent and Light-Emitting Diode (LED) Light Sources

2019 ◽  
Vol 96 (4) ◽  
pp. 396-402 ◽  
Author(s):  
Lili Jiang ◽  
Ziquan Wang ◽  
Guanghui Jin ◽  
Dianqiu Lu ◽  
Xuezhan Li
Keyword(s):  
Light Emitting Diode ◽  
Light Sources ◽  
Light Emitting ◽  
Led Light ◽  
Potato Plantlets

scholarly journals Dynamics of growth parameters of Gentiana lutea L. in vitro plants under different lighting conditions

2021 ◽  
Vol 28 ◽  
pp. 58-65
Author(s):  
L. R. Hrytsak ◽  
M. Z. Prokopiak ◽  
O. Yu. Mayorova ◽  
Kh. M. Kolisnyk ◽  
N. M. Drobyk
Keyword(s):  
Growth Parameters ◽  
Spectral Composition ◽  
Light Flux ◽  
In Vitro Plants ◽  
Gentiana Lutea ◽  
Plant Cultivation ◽  
In Situ Conditions ◽  
Flux Intensity ◽  
Cultivation In Vitro

Aim. Study of the dynamics Gentiana lutea L. plant growth processes in vitro depending on the light regime changes of their cultivation in order to develop a scheme to increase their adaptive potential. Methods. Methods of plant cultivation in vitro, biometric method, as well as ANOVA variance analysis and middle group analysis in pairs using the Tukey test (Tukey test) were used. Results. It is shown that the cultivation of G. lutea plants in vitro using 25 W/m2 light flux intensity in the region of photosynthetically active radiation and the ratio of blue (Eb): green (Eg): red (Er) ranges = 41.8%: 42.7 %: 15.5% triggers non-specific photomorphogenesis reactions for intact plants, which lead to poor root system development, stem elongation, formation of small leaves with a thin leaf blade, overall low productivity and low adaptation potential of G. lutea plants to ex vitro and in situ conditions. Increasing the light flux intensity to 44 W/m2 and increasing the red wave proportion up to 20.3% allows not only to improve the bioproductivity of G. lutea plants which are cultivated in vitro, but also to increase the coefficient of microclonal reproduction without the additional use of exogenous growth regulators. The greatest growth of the aboveground and underground parts, increase in effective leaf surface are observed in vitro plants during cultivation at 135 W/m2 light flux intensity and spectral composition Eb: Eg: Er = 29.5%: 32.5%: 38.0%. Conclusions. It is possible to improve plant bioproductivity by changing the light conditions of plant cultivation in vitro, and, accordingly, to increase the adaptive potential to ex vitro and in situ conditions. Keywords: Gentiana lutea L., in vitro plants, light flux intensity, spectral composition, growth parameters.

Spectral Estimation of Multiple Light Sources based on Highlight Detection

2020 ◽  
Vol 64 (5) ◽  
pp. 50408-1-50408-9
Author(s):  
Shoji Tominaga ◽  
Keita Hirai ◽  
Takahiko Horiuchi
Keyword(s):  
Light Source ◽  
Power Distribution ◽  
Spectral Power ◽  
Light Emitting Diode ◽  
Spectral Estimation ◽  
Spectral Composition ◽  
Image Data ◽  
Light Sources ◽  
Relaxation Labeling ◽  
Highlight Detection

Abstract The authors discuss the spectral estimation of multiple light sources from image data in a complex illumination environment. An approach is proposed to effectively estimate illuminant spectra and the corresponding light sources based on highlight areas that appear on dielectric object surfaces. First, the authors develop a highlight detection method using two types of convolution filters with Gaussian distributions, center-surround and low-pass filters. This method is available even for white surfaces, and it is independent of object color and of viewing and incidence angles. Second, they present an algorithm for estimating the illuminant spectra from extracted highlight areas. Each specular highlight area has a spectral composition corresponding to only one light source among multiple light sources. The spectral image data are projected onto a two-dimensional subspace, where a linear cluster in pixel distribution is detected for each highlight area. Third, the relative positional relationship between highlight areas among different object surfaces is used to identify the light sources on each surface. The authors develop an algorithm based on probabilistic relaxation labeling. The light source for each highlight and the corresponding spectral-power distribution are determined from the iterative labeling process. Finally, the feasibility of the proposed approach is examined in an experiment using a real complex environment, where dielectric objects are illuminated by multiple light sources of light-emitting diode, fluorescence, and incandescence.

scholarly journals The effect of led light sources with varied spectral composition on the in vitro rooting bility of garden strawberry (Fragaria × ananassa)

2019 ◽  
Vol 180 (1) ◽  
pp. 33-37 ◽  
Author(s):  
I. A. Byadovsky
Keyword(s):  
Red Light ◽  
Spectral Composition ◽  
Light Flux ◽  
Light Sources ◽  
Fragaria Ananassa ◽  
Fluorescent Lamp ◽  
Led Light ◽  
Rooting Ability ◽  
Strawberry Cultivars

Consideration is given to the possibility of using LED light sources with different ratios of red (650 - 660 nm), orange (600 - 610 nm) and blue (440 - 450 nm) light in the light flux in the rooting stage of garden strawberry (Fragaria×ananassa(Duch. ex Weston) Duch. ex Rozier) grownin vitro. The study covered a sizable diversity of strawberry cultivars (selected from thein vitrocollection): ‘Borovitskaya’, ‘Vityaz’, ‘Zenga Zengana’, ‘Khoney’, ‘Tsaritsa’, ‘Kubata’, ‘Dukat’, ‘Marmalada’, ‘Valenta’, ‘Rusich’, ‘Krasavitsa Zagorya’, ‘Neznakomka’ and ‘Roksana’, thus ensuring quite diversified results. It was established that the use of LED sources with an increased content of red light in the light flux increased the rooting percentage (1.15-1.25 times) and the number of roots per one microcutting (1.29-1.77 times) inin vitroculture, as compared with fluorescent lamp lighting. It was observed that with different spectral compositions of light the rooting ability of microcuttings was cultivar-specific.

scholarly journals THE EFFECT OF VARIOUS NUTRIENT MEDIA ON PLANTS OF POTATO VARIETIES MEMORY ROGACHEV AND KET IN VITRO

2018 ◽  
pp. 39-45
Author(s):  
O. O. Novikov ◽  
M. S. Romanov ◽  
N. I. Leonova ◽  
E. V. Haksar ◽  
Yu. V. Chudinova
Keyword(s):  
Component Composition ◽  
Nutrient Media ◽  
Culture In Vitro ◽  
Potato Varieties ◽  
Potato Plants

The influence of nutrient media of different component composition on ket potato varieties and Rogachev Memory was studied. It is shown that in the conditions of culture in vitro potato plants of the studied varieties show variety specificity when cultivated on nutrient media of different composition.

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