scholarly journals Modifications of the mini-flotac technique used on cattle and sheep feces to facilitate its use in field routines

2021 ◽  
Vol 19 ◽  
pp. 1
Author(s):  
Vinícius Vieira De Paiva ◽  
Lívia Mendonça de Aguiar ◽  
Gismar Silva Vieira ◽  
Fernanda Rosalinski-Moraes
Keyword(s):  
Flotac Technique ◽  
Sheep Feces ◽  
Cattle And Sheep

scholarly journals Occurrence and molecular characterization of Cryptosporidium sp. in sheep

2017 ◽  
Vol 38 (4) ◽  
pp. 1917 ◽  
Author(s):  
Alessandra Snak ◽  
Flavia Roberta Smiderle ◽  
Nelson Luis Mello Fernandes ◽  
Arielle Aparecida Lara ◽  
Felipe Gustavo Garcia ◽  
...  
Keyword(s):  
Farm Animals ◽  
Management Systems ◽  
Management Styles ◽  
Sequencing Data ◽  
Worldwide Distribution ◽  
Stool Samples ◽  
Sheep Feces ◽  
Fecal Smear ◽  
Cattle And Sheep

Considered a zoonosis of utmost importance, cryptosporidiosis has a worldwide distribution and can infect mammals, birds, reptiles, and amphibians. It is caused by a highly resistant protozoan present in the environment and can cause death in immunosuppressed individuals and pups, as well as in farm animals such as cattle and sheep, generating losses. The aim of this study was to investigate the presence of Cryptosporidium spp. in sheep feces from the farms of Western Paraná, which have different management styles, and compare the results with their respective management methods. One hundred and forty-four stool samples were collected (69 from Property 1 and 75 from Property 2) and analyzed using a fecal smear on slides after staining by the modified Ziehl-Neelsen method. Samples tested positive by this method were subjected to nested PCR and the products obtained were sent for sequencing to determine the species. While 82.60% of the samples from Property 1 were tested positive, only 36% of the samples from Property 2 were tested positive. On analyzing the sequencing data, it was observed that the Cryptosporidium species of samples from Property 1 showed high similarity to Cryptosporidium xiaoi and those from Property 2, to Cryptosporidium ubiquitum. The reason for divergence in results can be attributed to differences in management systems adopted by each property, thus showing the importance of detecting carrier animals, as they can contaminate the environment, especially the water sources, and spread the disease to humans and other animals.

scholarly journals The Gastrointestinal Tract as a Potential Infection Reservoir of Digital Dermatitis-Associated Treponemes in Beef Cattle and Sheep

2015 ◽  
Vol 81 (21) ◽  
pp. 7460-7469 ◽  
Author(s):  
L. E. Sullivan ◽  
S. D. Carter ◽  
J. S. Duncan ◽  
D. H. Grove-White ◽  
J. W. Angell ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Gastrointestinal Tract ◽  
Beef Cattle ◽  
Dairy Cattle ◽  
Gi Tract ◽  
Digital Dermatitis ◽  
Content Type ◽  
Treponemal Infection ◽  
Sheep Feces ◽  
Cattle And Sheep

ABSTRACTDigital dermatitis (DD) is an important cause of lameness in dairy cattle worldwide. It has now been reported in beef cattle and also sheep (contagious ovine digital dermatitis [CODD]). ThreeTreponemaphylogroups are consistently isolated from lesions,Treponema medium-like,Treponema phagedenis-like, andTreponema pedis. The gastrointestinal (GI) tract and feces are suggested sites of treponemal infection in dairy cattle; however, isolation of DD-associated treponemes from these areas has previously failed. This study surveyed gingival tissues, rectal tissues, and feces of beef cattle and sheep for the molecular presence (PCR) and isolation of the three cultivable DD-treponeme phylogroups. Of the sheep gingival (n= 40) and rectal (n= 40) tissues, 1/40 gingival tissues was positive for DD-associated treponemes (T. pedis), as were 3/40 rectal tissues (one containingT. medium-like and two containingT. pedis). No DD-associated treponeme DNA was amplified from beef cattle rectal tissues (n= 40); however, 4/40 beef gingival tissues were positive for DD-associated treponemes (all containingT. phagedenis-like). AT. phagedenis-like DD-associated treponeme was isolated from the rectal tissue of a CODD symptomatic sheep. Beef cattle (n= 41) and sheep (n= 79) feces failed to amplify DD-associatedTreponemaDNA. Twenty-two treponemes were isolated from sheep feces; however, upon phylogenetic analysis, these clustered with the considered nonpathogenic treponemes. This study detected DD-associated treponemes in the GI tract tissues of sheep and beef cattle and successfully isolated a DD-associated treponeme from ruminant rectal tissue. This gives evidence that the GI tract is an important infection reservoir of DD-associated treponemes in multiple DD-infected species.

scholarly journals Stx1 and Stx2 subtyping and antimicrobial resistance in Shiga toxin-producing Escherichia coli (STEC) isolates from cattle and sheep feces in the Southeastern region of the State of Goiás, Brazil

2021 ◽  
Vol 41 ◽  
Author(s):  
Bruna R. Arrais ◽  
Ângela V.B.A. Silveira ◽  
Angélica F. Oliveira ◽  
Nayara C. Barbosa ◽  
Ariel E. Stella ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Shiga Toxin ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Pathogenic Potential ◽  
Significant Difference ◽  
Sheep Feces ◽  
Cattle And Sheep

ABSTRACT: The present study was aimed at subtyping of Stx1 and Stx2 genes and characterization of antimicrobial resistance in 106 Shiga toxin-producing Escherichia coli (STEC) strains isolated from cattle and sheep feces. PCR was used to determine the subtypes, and the disk-diffusion method was used to evaluate the antimicrobial resistance. Ten antibiotics from five different classes were tested. Among the isolates of bovine origin, two subtypes of Stx1 (Stx1a and Stx1c), and four subtypes of Stx2 (Stx2a, Stx2b, Stx2c, and Stx2d) were identified. In isolates of sheep origin, two subtypes of Stx1 (Stx1a and Stx1c), and four subtypes of Stx2 (Stx2a, Stx2b, Stx2c, and Stx2 g) were identified. The results obtained suggest the presence of high diversity in Stx1 and Stx2 genes. Further, 96.6% (57/59) of bovine fecal strains and 89.4% (42/47) of sheep fecal strains showed resistance to at least one tested antibiotic. In both animal species, most strains were multidrug-resistant (MDR) (67.8% in cattle and 59.6% in sheep), with no significant difference between host animals. Adult animals were eight times more likely to have STEC with greater pathogenic potential. STEC with the highest pathogenic potential were three times more likely to be multidrug-resistant than STEC with the lowest pathogenic potential. The data reported in this study suggests the occurrence of strains with high potential pathogenicity in the region studied. Therefore, the ruminants of this region are carriers of strains that can cause infections in humans.

Differential diagnosis of bluetongue in cattle and sheep

In Practice ◽  
2008 ◽  
Vol 30 (5) ◽  
pp. 242-251 ◽  
Author(s):  
Susanna Williamson ◽  
Nick Woodger ◽  
Karin Darpel
Keyword(s):  
Differential Diagnosis ◽  
Cattle And Sheep

scholarly journals Antibodies to Borrelia Spirochetes in Sera from Swedish Cattle and Sheep

1986 ◽  
Vol 27 (4) ◽  
pp. 479-485
Author(s):  
A. Hovmark ◽  
E. Åsbrink ◽  
O. Schwan ◽  
B. Hederstedt ◽  
D. Christensson
Keyword(s):  
Cattle And Sheep

scholarly journals Response patterns in adventitial layer of Echinococcus granulosus sensu stricto cysts from naturally infected cattle and sheep

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Christian Hidalgo ◽  
Caroll Stoore ◽  
María Soledad Baquedano ◽  
Ismael Pereira ◽  
Carmen Franco ◽  
...  
Keyword(s):  
Immune Response ◽  
Echinococcus Granulosus ◽  
Cystic Echinococcosis ◽  
Direct Contact ◽  
Sensu Stricto ◽  
Response Patterns ◽  
Granulomatous Reaction ◽  
Adventitial Layer ◽  
Laminated Layer ◽  
Cattle And Sheep

AbstractCystic echinococcosis is a zoonotic disease caused by the metacestode of Echinococcus granulosus sensu lato. The disease is characterized by the development of cystic structures inside viscera of the intermediate host, mainly liver and lungs. These cysts are formed by three layers: germinal, laminated, and adventitial layer, the latter being the local host immune response. Metacestodes that develop protoscoleces, the infective stage to the definitive host, are termed fertile, whereas cysts that do not produce protoscoleces are termed non-fertile. Sheep usually harbor fertile cysts while cattle usually harbor non-fertile cysts. Adventitial layers with fibrotic resolution are associated to fertile cysts, whereas a granulomatous reaction is associated with non-fertile cysts. The aim of this study was to analyze cellular distribution in the adventitial layer of fertile and non-fertile E. granulosus sensu stricto cysts found in liver and lungs of cattle and sheep. A total of 418 cysts were analyzed, 203 from cattle (8 fertile and 195 non-fertile) and 215 from sheep (64 fertile and 151 non-fertile). Fertile cysts from cattle showed mixed patterns of response, with fibrotic resolution and presence of granulomatous response in direct contact with the laminated layer, while sheep fertile cysts always displayed fibrotic resolution next to the laminated layer. Cattle non-fertile cysts display a granulomatous reaction in direct contact with the laminated layer, whereas sheep non-fertile cysts display a granulomatous reaction, but in direct contact with the fibrotic resolution. This shows that cattle and sheep cystic echinococcosis cysts have distinct local immune response patterns, which are associated to metacestode fertility.

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