scholarly journals Ammonium sulfate precipitation combined with liquid chromatography is sufficient for purification of bovine serum albumin that is suitable for most routine laboratory applications

2013 ◽  
Vol 1 (1) ◽  
pp. 3 ◽  
Author(s):  
Odutayo O Odunuga ◽  
Alina Shazhko
Keyword(s):  
Liquid Chromatography ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Ammonium Sulfate ◽  
Bovine Serum ◽  
Routine Laboratory ◽  
Ammonium Sulfate Precipitation

scholarly journals THE NATURE OF ANTIGEN-ANTIBODY COMPLEXES FORMED IN RABBITS DURING AN IMMUNE RESPONSE TO BOVINE SERUM ALBUMIN

1958 ◽  
Vol 107 (5) ◽  
pp. 653-663 ◽  
Author(s):  
William O. Weigle
Keyword(s):  
Immune Response ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Ammonium Sulfate ◽  
Bovine Serum ◽  
Ammonium Sulfate Precipitation ◽  
Antibody Synthesis ◽  
Circulating Antigen ◽  
Antigen Antibody

The immune elimination of soluble BSA, following an intravenous injection, is accompanied by the appearance of circulating antigen-antibody complexes. The pattern of the appearance of circulating antigen-antibody complexes and the immune elimination of antigen probably depends on the amount of antigen injected, the rate of antibody synthesis, and perhaps, the quality of antibody produced. There is no relationship between the I* antigen-antibody complexes detected during the immune response in rabbits by ammonium sulfate precipitation and the material precipitated from immune sera as a result of treatment with alkali. Alkali-precipitable material present in the serum of rabbits at a time when I* antigen is also present contain at most only traces of the antigen.

Rapid radioimmunoassay of total urinary estriol.

1976 ◽  
Vol 22 (5) ◽  
pp. 611-615 ◽  
Author(s):  
D W Anderson ◽  
U Goebelsmann
Keyword(s):  
Urinary Tract ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Ammonium Sulfate ◽  
Bovine Serum ◽  
Room Temperature ◽  
Urine Specimen ◽  
Ammonium Sulfate Precipitation ◽  
In Pregnancy

Abstract We describe a radioimmunoassay for total urinary estriol in pregnancy. A 25-mul aliquot of the urine specimen is acid hydrolyzed, neutralized, and diluted before assay. We use rabbit antisera against estriol-6-(O-carboxymethyl)oxime/bovine serum albumin and ammonium sulfate precipitation at room temperature. Results are unaffected by glucose or methenamine mandelate, a urinary tract antiseptic. Using semi-automatic pipetting equipment, one laboratory technologist can complete 50 assays within 8 h. This technique is both reliable and convenient and should decrease the expense of routine estriol assays.

scholarly journals Evaluation of Bovine Serum Albumin as a Fluorescence Enhancement Reagent in Liquid Chromatography.

1995 ◽  
Vol 11 (5) ◽  
pp. 743-747 ◽  
Author(s):  
Junichi ISHIDA ◽  
Kazutomo ABE ◽  
Masaru NAKAMURA ◽  
Masatoshi YAMAGUCHI
Keyword(s):  
Liquid Chromatography ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fluorescence Enhancement
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