scholarly journals APPLICATION OF THIN LAYER CHROMATOGRAPHY IN EXAMINATION OF THE BIOLOGICAL ACTIVITY PARAMETERS OF AZO DYES

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Đeni Vaštag ◽  
Suzana Apostolov ◽  
Borko Matijević ◽  
Gorana Mrđan
Keyword(s):  
Biological Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Azo Dyes ◽  
Linear Regression Analysis ◽  
Log P ◽  
Synthetic Dyes ◽  
Organic Modifiers ◽  
Wide Range ◽  
Layer Chromatography

Thanks to the wide range of applications, azo dyes have a special place among synthetic dyes. Also, the application of azo dyes is conditioned by their impact on humans and the environment. Lipophilicity as one of the most important molecular descriptors indicating the potential biological activity of the compound is determined experimentally for selected derivatives of azo dyes using reversed phase thin-layer chromatography (RP TLC18 F254s) in the mixtures of water and two organic modifiers. By using the relevant software packages for the studied azo dyes, the values of partition coefficient (log P) were calculated as standard measure of lipophilicity, important pharamacokinetic predictors and the values of the effective concetration (EC50) as the criteria of acute toxicity for different test organisms. The relationships between chromatographic parameters (RM 0 and m) and calculated biological activity parameters were studied by linear regression analysis.

scholarly journals STUDYING OF THE LIPOPHILICITY AND TOXICITY OF DIPHENYLACETAMIDE DERIVATIVES

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Suzana Apostolov ◽  
Đenđi Vaštag ◽  
Borko Matijević ◽  
Gorana Mrđan
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Reversed Phase ◽  
Biologically Active ◽  
Log P ◽  
Organic Modifiers ◽  
Modern Approach ◽  
Software Packages ◽  
Test Organisms ◽  
Layer Chromatography

Modern approach in the study of biologically active compounds includes the establishment of relationships between molecular structure, physicochemical properties and the behavior which studied compound can manifest in the biological medium. These examinantions are performed in the early stages of the design of future bioactive agent and require the knowledge of molecular descriptors that can point to its biological activity, including lipophilicity which occupies a key position. For the series of diphenylacetamide derivatives, lipophilicity is determined experimentally by thin-layer chromatography on reversed phase (RP TLC18 F254s), in mixtures of water and various organic modifiers and computationally, by using the relevant software packages. In order to estimate the potential acute toxicity of the tested diphenylacetamide derivatives, their effective concentrations, EC50, on the selected test organisms have been determined. Experimentally determined lipophilicity (RM 0 and m) is correlated with a standard measure of lipophilicity (log P), as well as with the selected parameters of toxicity. Thus it has been found that thin-layer chromatography on reversed phase can be used reliably for describing the lipophilicity and for the evaluation of the toxic effects of diphenylacetamide derivatives.

Phytochemical, Analytical and Medicinal Studies of Holoptelea integrifolia Roxb. Planch - A Review

2019 ◽  
Vol 5 (4) ◽  
pp. 270-277 ◽  
Author(s):  
Vijay Kumar ◽  
Simranjeet Singh ◽  
Ragini Bhadouria ◽  
Ravindra Singh ◽  
Om Prakash
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Toxicological Studies ◽  
Wide Range ◽  
Layer Chromatography

Holoptelea integrifolia Roxb. Planch (HI) has been used to treat various ailments including obesity, osteoarthritis, arthritis, inflammation, anemia, diabetes etc. To review the major phytochemicals and medicinal properties of HI, exhaustive bibliographic research was designed by means of various scientific search engines and databases. Only 12 phytochemicals have been reported including biologically active compounds like betulin, betulinic acid, epifriedlin, octacosanol, Friedlin, Holoptelin-A and Holoptelin-B. Analytical methods including the Thin Layer Chromatography (TLC), High-Performance Thin Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography With Mass Spectral (LC-MS) analysis have been used to analyze the HI. From medicinal potency point of view, these phytochemicals have a wide range of pharmacological activities such as antioxidant, antibacterial, anti-inflammatory, and anti-tumor. In the current review, it has been noticed that the mechanism of action of HI with biomolecules has not been fully explored. Pharmacology and toxicological studies are very few. This seems a huge literature gap to be fulfilled through the detailed in-vivo and in-vitro studies.

scholarly journals TLC analysis of some phenolic compounds in kombucha beverage

2004 ◽  
pp. 199-205 ◽  
Author(s):  
Radomir Malbasa ◽  
Eva Loncar ◽  
Ljiljana Kolarov
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Green Tea ◽  
Black Tea ◽  
Wide Range ◽  
Tea Fungus ◽  
Natural Phenolic Compounds ◽  
Tlc Analysis ◽  
Layer Chromatography

Black and green tea contains a wide range of natural phenolic compounds Flavanoids and their glycosides, catechins and the products of their condensation, and phenolic acids are the most important. Kombucha beverage is obtained by fermentation of tea fungus on black or green tea sweetened with sucrose. The aim of this paper was to investigate the composition of some phenolic compounds, catechin, epicatechin, quercetin, myricetin, gallic and tanic acid, and monitoring of their status during tea fungus fermentation. The method used for this study was thin layer chromatography with two different systems. The main phenolic compounds in the samples with green tea were catechin and epicatechin, and in the samples with black tea it was quercetin.

scholarly journals Reversed-phase thin-layer chromatography behavior of aldopentose derivatives

2012 ◽  
Vol 66 (3) ◽  
pp. 365-372 ◽  
Author(s):  
Dragana Livaja-Popovic ◽  
Eva Loncar ◽  
Lidija Jevric ◽  
Radomir Malbasa
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Chromatographic Behavior ◽  
Log P ◽  
Retention Factors ◽  
Mobile Phases ◽  
Acetone Water ◽  
Layer Chromatography

Quantitative structure-retention relationships (QSRR) have been used to study the chromatographic behavior of some aldopentose. The behavior of aldopentose derivatives was investigated by means of the reversed-phase thin-layer chromatography (RP TLC) on the silica gel impregnated with paraffin oil stationary phases. Binary mixtures of methanol-water, acetone-water and dioxane-water were used as mobile phases. Retention factors, RM0, corresponding to zero percent organic modifier in the aqueous mobile phase was determined. Lipophilicity C0 was calculated as the ratio of the intercept and slope values. There was satisfactory correlation between them and log P values calculated using different theoretical procedures. Some of these correlations offer very good predicting models, which are important for a better understanding of the relationships between chemical structure and retention. The study showed that the hydrophobic parameters RM0 and C0 can be used as a measures of lipophilicity of investigated compounds.

scholarly journals Development and Evaluation of Thin-Layer Chromatography-Digital Image-Based Analysis for the Quantitation of the Botanical Pesticide Azadirachtin in Agricultural Matrixes and Commercial Formulations: Comparison with ELISA

2007 ◽  
Vol 90 (3) ◽  
pp. 857-863 ◽  
Author(s):  
Penmatsa Tanuja ◽  
Namburi Venugopal ◽  
Rao Beedu Sashidhar
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Digital Image ◽  
Linear Regression Analysis ◽  
Calibration Plot ◽  
Reference Standard ◽  
Botanical Pesticide ◽  
Significant Difference ◽  
Derivatizing Agent ◽  
Layer Chromatography

Abstract A simple thin-layer chromatography-digital image-based analytical method has been developed for the quantitation of the botanical pesticide, azadirachtin. The method was validated by analyzing azadirachtin in the spiked food matrixes and processed commercial pesticide formulations, using acidified vanillin reagent as a postchromatographic derivatizing agent. The separated azadirachtin was clearly identified as a green spot. The Rf value was found to be 0.55, which was similar to that of a reference standard. A standard calibration plot was established using a reference standard, based on the linear regression analysis [r2 = 0.996; y = 371.43 + (634.82)x]. The sensitivity of the method was found to be 0.875 g azadirachtin. Spiking studies conducted at the 1 ppm (g/g) level in various agricultural matrixes, such as brinjal, tomato, coffee, and cotton seeds, revealed the recoveries of azadirachtin in the range of 6792%. Azadirachtin content of commercial neem formulations analyzed by the method was in the range of 1901825 ppm (g/mL). Further, the present method was compared with an immunoanalytical method enzyme-linked immonosorbent assay developed earlier in our laboratory. Statistical comparison of the 2 methods, using Fischer's F-test, indicated no significant difference in variance, suggesting that both methods are comparable.

scholarly journals Fluorescein colchicine. Synthesis, purification, and biological activity.

1978 ◽  
Vol 76 (3) ◽  
pp. 619-627 ◽  
Author(s):  
J I Clark ◽  
D Garland
Keyword(s):  
Spectral Analysis ◽  
Biological Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Fluorescein Isothiocyanate ◽  
Significant Loss ◽  
Colchicine Derivative ◽  
Layer Chromatography ◽  
Kinetics Of ◽  
Brain Tubulin

The synthesis of a fluorescent colchicine derivative permits the localization of colchicine-binding receptors in cells. Fluorescein colchicine (FC) was prepared by the addition of fluorescein isothiocyanate to deacetyl colchicine. The product, FC, was separated from the reactants by thin-layer chromatography (TLC). The purity of FC was demonstrated by TLC, UV spectral analysis, and analysis of the kinetics of photodecomposition. FC inhibited [3H] colchicine binding to purified brain tubulin. The biological activity of FC was compared to the activity of unlabeled colchicine on mitosis, motility, secretion, and myogenesis. The effects of FC were identical to those of unlabeled colchicine in all biological systems tested. The results demonstrate that FC may be substituted for colchicine in biological experiments without significant loss in specificity or effectiveness.

Determination of Aflatoxins, Ochratoxin A, and Zearalenone in Mixed Feeds, with Detection by Thin Layer Chromatography or High Performance Liquid Chromatography

1981 ◽  
Vol 64 (6) ◽  
pp. 1356-1363 ◽  
Author(s):  
Mary V Howell ◽  
Philip W Taylor
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Wide Range ◽  
Mixed Feeds ◽  
Layer Chromatography

Abstract A sensitive, reliable, and economical method for the determination of 6 mycotoxins in mixed feeds is described. The feed is extracted with chloroform-water and the extract is cleaned up by using a disposable Sep-Pak silica cartridge. The procedure requires less time (15 min from sample extraction to extract preparation) and less solvent (approximately one-tenth) compared with conventional methods and is suitable for a fast, economical screen. Additional cleanup procedures, involving dialysis or extraction into base, are described for samples containing high levels of interfering compounds. Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) with fluorescence detection are described for identification and estimation of mycotoxins. The method has been applied to a wide range of mixed feeds, including laboratory animal diets, and raw materials. The limit of detection is 1 μg/kg for all mycotoxins measured by HPLC.

Sign in / Sign up

Export Citation Format

Share Document