scholarly journals Isolation and characterization of psychrotrophic proteolytic bacteria from landfill site under temperate climatic conditions of Kashmir Himalaya

2019 ◽  
Author(s):  
Basharat Hamid ◽  
Arshid Jehangir ◽  
Zahoor Ahmad Baba ◽  
Muneer Ahmad Wani ◽  
Imran Khan
Keyword(s):  
Skim Milk ◽  
Industrial Applications ◽  
Economic Benefits ◽  
Climatic Conditions ◽  
Landfill Site ◽  
Climatic Regions ◽  
Isolation And Characterization ◽  
Cold Active ◽  
Proteolytic Bacteria

The temperate climatic regions face the problem of waste accumulation due to lower environmental temperatures. However, these regions harbor cold active microbes viz. psychrotrophic proteolytic bacteria that play an important role in the degradation of protenaceous materials of the waste stream. Hence in the present study psychrotrophic proteolytic bacteria were isolated from waste samples collected from landfill site by using random sampling method under environmental temperature of 10oC. By using serial dilution and spread plate technique a total of 8 morphologically different psychrotrophic proteolytic bacteria were isolated on skim milk agar media at pH of 7.0 and temperature of 10°C after 48hours. Under in-vitro conditions all the isolates produced significant quantities of protease over the control and diameters of hydrolysis zones ranged between 2 to 18 mm at temperature range of 5 to 20oC and after 72 hours. The corresponding quantitative protease activities of the isolates was significant that ranged between 0.5 to 2.25 U/ml and the isolate PB2 was most efficient with highest protease activity of 2.25U/ml at 20oC. Based on 16SrRNA analysis the isolate was identified as Pseudomonas florescence with 96% similarity. It was concluded that the isolates can grow in wide ranges of temperature and could be used for enhanced decomposition of organic wastes during lower temperature conditions in cold regions. Further the isolates could have industrial applications due to the production of cold active proteases that would help economic benefits through energy conservation.

scholarly journals Isolation and characterization of psychrotrophic proteolytic bacteria from landfill site under temperate climatic conditions of Kashmir Himalaya

2019 ◽  
Author(s):  
Basharat Hamid ◽  
Arshid Jehangir ◽  
Zahoor Ahmad Baba ◽  
Muneer Ahmad Wani ◽  
Imran Khan
Keyword(s):  
Skim Milk ◽  
Industrial Applications ◽  
Economic Benefits ◽  
Climatic Conditions ◽  
Landfill Site ◽  
Climatic Regions ◽  
Isolation And Characterization ◽  
Cold Active ◽  
Proteolytic Bacteria

The temperate climatic regions face the problem of waste accumulation due to lower environmental temperatures. However, these regions harbor cold active microbes viz. psychrotrophic proteolytic bacteria that play an important role in the degradation of protenaceous materials of the waste stream. Hence in the present study psychrotrophic proteolytic bacteria were isolated from waste samples collected from landfill site by using random sampling method under environmental temperature of 10oC. By using serial dilution and spread plate technique a total of 8 morphologically different psychrotrophic proteolytic bacteria were isolated on skim milk agar media at pH of 7.0 and temperature of 10°C after 48hours. Under in-vitro conditions all the isolates produced significant quantities of protease over the control and diameters of hydrolysis zones ranged between 2 to 18 mm at temperature range of 5 to 20oC and after 72 hours. The corresponding quantitative protease activities of the isolates was significant that ranged between 0.5 to 2.25 U/ml and the isolate PB2 was most efficient with highest protease activity of 2.25U/ml at 20oC. Based on 16SrRNA analysis the isolate was identified as Pseudomonas florescence with 96% similarity. It was concluded that the isolates can grow in wide ranges of temperature and could be used for enhanced decomposition of organic wastes during lower temperature conditions in cold regions. Further the isolates could have industrial applications due to the production of cold active proteases that would help economic benefits through energy conservation.

scholarly journals Isolation and characterization of psychrotrophic proteolytic bacteria from landfill site under temperate climatic conditions of Kashmir Himalaya

2018 ◽  
Author(s):  
Basharat Hamid ◽  
Arshid Jehangir ◽  
Zahoor Ahmad Baba ◽  
Muneer Ahmad Wani ◽  
Imran Khan
Keyword(s):  
Skim Milk ◽  
Industrial Applications ◽  
Economic Benefits ◽  
Climatic Conditions ◽  
Landfill Site ◽  
Climatic Regions ◽  
Isolation And Characterization ◽  
Cold Active ◽  
Proteolytic Bacteria

The temperate climatic regions face the problem of waste accumulation due to lower environmental temperatures. However, these regions harbor cold active microbes viz. psychrotrophic proteolytic bacteria that play an important role in the degradation of protenaceous materials of the waste stream. Hence in the present study psychrotrophic proteolytic bacteria were isolated from waste samples collected from landfill site by using random sampling method under environmental temperature of 10oC. By using serial dilution and spread plate technique a total of 8 morphologically different psychrotrophic proteolytic bacteria were isolated on skim milk agar media at pH of 7.0 and temperature of 10°C after 48hours. Under in-vitro conditions all the isolates produced significant quantities of protease over the control and diameters of hydrolysis zones ranged between 2 to 18 mm at temperature range of 5 to 20oC and after 72 hours. The corresponding quantitative protease activities of the isolates was significant that ranged between 0.5 to 2.25 U/ml and the isolate PB2 was most efficient with highest protease activity of 2.25U/ml at 20oC. Based on 16SrRNA analysis the isolate was identified as Pseudomonas florescence with 96% similarity. It was concluded that the isolates can grow in wide ranges of temperature and could be used for enhanced decomposition of organic wastes during lower temperature conditions in cold regions. Further the isolates could have industrial applications due to the production of cold active proteases that would help economic benefits through energy conservation.

scholarly journals Isolasi dan Karakterisasi Bakteri Proteolitik yang Berasosiasi dengan Lamun Enhalus acoroides di Pantai Bama, Taman Nasional Baluran, Situbondo, Jawa Timur [Isolation and Characterization Proteolytic Bacteria which is Associated with Sea Grass (Enhalus acoroides) in Bama Beach, Baluran National Park, Situbondo, East Java].

2018 ◽  
Vol 10 (1) ◽  
pp. 8 ◽  
Author(s):  
Rachmat Rizaldi ◽  
Woro Hastuti Setyantini ◽  
Sudarno Sudarno
Keyword(s):  
National Park ◽  
Sea Water ◽  
Skim Milk ◽  
Survey Method ◽  
Bacillus Sp ◽  
Bacterial Isolates ◽  
Enhalus Acoroides ◽  
Isolation And Characterization ◽  
Protease Enzyme ◽  
Proteolytic Bacteria

AbstrakLamun adalah tumbuhan sejati yang hidup di perairan pantai yang kurang dimanfaatkan dalam bidang perikanan, selain sebagai bioindikator kualitas air laut. Beberapa mikroorganisme yang berasosiasi dengan lamun Enhalus acoroides antara lain benthos, kapang, bakteri dan plankton. Bakteri proteolitik merupakan bakteri yang mampu menghasilkan enzim protease. Enzim perotese merupakan enzim yang paling banyak digunakan dalam kehidupan. Bakteri merupakan sumber enzim yang paling banyak digunakan dibandingkan dengan tanaman dan hewan. Tujuan penelitian ini adalah untuk mengetahui jenis isolat bakteri proteolitik yang berasosiasi dengan lamun Enhalus acoroides di Taman Nasional Baluran, Situbondo. Penelitian ini menggunakan metode survei dengan dianalisis secara deskriptif dengan bantuan tabel dan gambar. Hasil yang peroleh menunjukkan bahwa terdapat 12 isolat bakteri yang berasosisasi dengan lamun Enhalus acoroides. Terdapat empat isolat yang tergolong sebagai bakteri proteolitik karena mampu mendagradasi kasein dalam media TSA + 2% NaCL yang ditambah 1 % susu skim, tampak dari pembentukan zona bening. Pengamatan morfologi koloni dan sel serta pengujian biokimia dari keempat isolat (EA-1, EA-2, EA-9 dan EA-10) terdapat kesamaan karakteristik dengan empat genus bakteri berturut-turut yaitu Staphylococcus sp., Plesiomonas shigeloides, Bacillus sp., Pseudomonas sp.AbstractSeagrass is a true living plants underutilized coastal waters in the field of fisheries, as well as bio-indicators of the quality of sea water. Some microorganisms associated with seagrass Enhalus acoroides among others benthos, fungi, bacteria and plankton. Proteolytic bacteria are bacteria that are capable of producing the enzyme protease. Protease enzyme is an enzyme that is most widely used in life. Bacteria are a source of enzymes that are most widely used compared to plants and animals. The purpose of this study was to determine the type of proteolytic bacterial isolates associated with seagrass Enhalus acoroides in Baluran National Park, Situbondo. This study used survey method with descriptive analysis with tables and figures. The results obtained that there are 12 bacterial isolates associated with seagrass Enhalus acoroides. There are four isolates were classified as proteolytic bacteria because it can degrade casein in TSA media + 2 % NaCL plus 1% skim milk which is evidenced by the formation of clear zones. Observations colony morphology and cells, as well as testing of Biochemistry of the four isolates (EA-1, EA-2, EA-9 and EA-10), were obtained, with similar characteristics to the four genera of bacteria in a row as follows Staphylococcus sp., Plesiomonas shigeloides, Bacillus sp., Pseudomonas sp. 

scholarly journals In vitro and in vivo Antiamoebic Potential of Actinopyga lecanora (Jaeger)

2015 ◽  
Vol 18 (2) ◽  
pp. 118-120 ◽  
Author(s):  
Vijai Lakshmi ◽  
Sheela Ghosal
Keyword(s):  
Entamoeba Histolytica ◽  
Symptomatic Relief ◽  
Pharmaceutical Journal ◽  
Climatic Conditions ◽  
Isolation And Characterization ◽  
Histolytica Infection ◽  
Antiamoebic Activity

Human amoebiasis, due to Entamoeba histolytica infection, is mainly associated with morbidity thus affecting the quality of life and pace of development in the countries with warm climatic conditions. So far, the available drugs provide only symptomatic relief and they are not devoid of side effects. This leads to obtain novel molecules from natural sources having antiamoebic activity. The methanol extract of Actinopyga lecanora (Jaeger) displayed antiamoebic activity. It showed MIC 125 ?g/ml in our in-vitro studies, but when it was tested in rats, it revealed 88% inhibition of trophozoites at the dose of 900 mg/kg body weight against Entamoeba histolytica. Further work is in progress for the isolation and characterization of active molecules.Bangladesh Pharmaceutical Journal 18(2): 118-120, 2015

scholarly journals Biodiversity of Pathogenic and Toxigenic Seed-Borne Mycoflora of Wheat in Egypt and Their Correlations with Weather Variables

Biology ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1025
Author(s):  
Yasser M. Shabana ◽  
Younes M. Rashad ◽  
Khalid M. Ghoneem ◽  
Nehal S. Arafat ◽  
Dalia G. Aseel ◽  
...  
Keyword(s):  
Relative Humidity ◽  
Management Strategies ◽  
Molecular Data ◽  
Fungal Species ◽  
Climatic Conditions ◽  
Biodiversity Indicators ◽  
Toxigenic Fungi ◽  
Climatic Regions ◽  
Pathogen Occurrence

Surveillance investigations for pathogenic and toxigenic fungi are important to refine our understanding of their epidemiology and help in predicting their outbreaks. During 2019, 198 samples of wheat grains were collected from 25 wheat-growing governorates in Egypt to detect and identify seed-borne mycoflora in vitro. Forty-four fungal species belonging to 20 genera were identified. Molecular data for these fungi were analyzed to construct a phylogenetic tree. Occurrence and biodiversity indicators were calculated. Two prevalent pathogens (average incidence > 40%) were Alternaria alternata and Cladosporium spp. Ustilago tritici was present in only seven of the 25 governorates, and less abundant than Tilletia tritici, the causal agent of stinking smut. Sinai governorate recorded the greatest species diversity, while the greatest species richness was in Qena and Sohag governorates. Canonical correspondence analysis of data for 20 fungal genera with temperature, relative humidity, precipitation, wind speed or solar radiation revealed that relative humidity was the most influential weather variable. It showed that occurrence and distribution of the 20 genera corresponded well with three out of four Egyptian climatic regions: Mediterranean, semi-arid, and arid. Knowing pathogen occurrence and distribution in Egypt is the first step to developing future disease management strategies to limit yield losses and improve food security. Despite this study being conducted on the wheat-growing areas in Egypt, our findings are useful for other wheat-growing countries that share the same climatic conditions. The correlation between a given fungus and the climatic variables can be useful in other ecosystems.

scholarly journals Antiamoebic Activity of Marine Sponge Spongia officinalis var. ceylonensis Dendy

2015 ◽  
Vol 17 (1) ◽  
pp. 38-42 ◽  
Author(s):  
Vijai Lakshmi ◽  
Sheela Ghosal
Keyword(s):  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
Climatic Conditions ◽  
Bioactive Molecules ◽  
Isolation And Characterization ◽  
Antiamoebic Activity ◽  
Spongia Officinalis

Human amoebiasis due to infection by Entamoeba histolytica is mainly associated with morbidity thus affecting the quality of life and pace of developmental activities of countries with warm climatic conditions. A consistently high global incidence of this disease has been reported. In an endeavor to identify novel molecules with potent antiamoebic activity and lower side-effect, the present study was designed to investigate antiamoebic activity in Spongia officinalis extracts/fractions followed by isolation and characterization of bioactive molecules. The methanol extract of the S. officinalis showed promising in vitro and in vivo antiamoebic activity. On further fractionation, the activity was localized in the n-butanol soluble fraction from which the major purified fraction was identified as a mixture of alkaloids (xestospongins and araguspongins) by LCMS studies of the n-butanol soluble fraction DOI: http://dx.doi.org/10.3329/bpj.v17i1.22312 Bangladesh Pharmaceutical Journal 17(1): 38-42, 2014

Enzyme Promiscuous Activity: How to Define it and its Evolutionary Aspects

2020 ◽  
Vol 27 (5) ◽  
pp. 400-410
Author(s):  
Valentina De Luca ◽  
Luigi Mandrich
Keyword(s):  
Gene Evolution ◽  
Sequence Divergence ◽  
High Specificity ◽  
Industrial Applications ◽  
Point Of View ◽  
Enzyme Promiscuity ◽  
Primary Activity ◽  
Starting Point ◽  
Main Activity

: Enzymes are among the most studied biological molecules because better understanding enzymes structure and activity will shed more light on their biological processes and regulation; from a biotechnological point of view there are many examples of enzymes used with the aim to obtain new products and/or to make industrial processes less invasive towards the environment. Enzymes are known for their high specificity in the recognition of a substrate but considering the particular features of an increasing number of enzymes this is not completely true, in fact, many enzymes are active on different substrates: this ability is called enzyme promiscuity. Usually, promiscuous activities have significantly lower kinetic parameters than to that of primary activity, but they have a crucial role in gene evolution. It is accepted that gene duplication followed by sequence divergence is considered a key evolutionary mechanism to generate new enzyme functions. In this way, promiscuous activities are the starting point to increase a secondary activity in the main activity and then get a new enzyme. The primary activity can be lost or reduced to a promiscuous activity. In this review we describe the differences between substrate and enzyme promiscuity, and its rule in gene evolution. From a practical point of view the knowledge of promiscuity can facilitate the in vitro progress of proteins engineering, both for biomedical and industrial applications. In particular, we report cases regarding esterases, phosphotriesterases and cytochrome P450.

PENGEMBANGAN TEKNOLOGI SELEKSI IN VITRO BERULANG (REPEAT CYCLING–IN VITRO SELECTION) TOLERAN STRES KEKERINGAN UNTUK PERBAIKAN MUTU GENETIK DAN PRODUKSI TANAMAN KEDELAI BERMIKORIZA

2017 ◽  
Vol 1 (1) ◽  
pp. 74-84
Author(s):  
Ahmad Riduan ◽  
Rainiyati Rainiyati ◽  
Yulia Alia
Keyword(s):  
Arbuscular Mycorrhizae ◽  
In Vitro Selection ◽  
Soil Samples ◽  
Single Spore ◽  
Isolation And Characterization ◽  
Single Spore Culture ◽  
Spore Culture ◽  
Glomus Sp

Every plant rhizospheres in any ecosystem there are various living microorganisms including Arbuscular Mycorrhizae Fungi (AMF).  An isolation and characterization is required to investigate the species or type of the AMF. This research was aimed at studying the isolation and characterization of AMF sporulation in soybean rhizospheres in Jambi Province. The results of evaluation on soil samples before trapping showed that there are spores from three genus of AMF twelve types Glomus , two types Acaulospora and one type of Enthrophospora.  Following single spore culture in soybean rhizosphere, 5 spore types were obtained:  Glomus sp-1, Glomus sp-4, Glomus sp-7, Glomus sp-8 Glomus sp-10.

Sign in / Sign up

Export Citation Format

Share Document