scholarly journals Isolasi dan Karakterisasi Bakteri Proteolitik yang Berasosiasi dengan Lamun Enhalus acoroides di Pantai Bama, Taman Nasional Baluran, Situbondo, Jawa Timur [Isolation and Characterization Proteolytic Bacteria which is Associated with Sea Grass (Enhalus acoroides) in Bama Beach, Baluran National Park, Situbondo, East Java].

2018 ◽  
Vol 10 (1) ◽  
pp. 8 ◽  
Author(s):  
Rachmat Rizaldi ◽  
Woro Hastuti Setyantini ◽  
Sudarno Sudarno
Keyword(s):  
National Park ◽  
Sea Water ◽  
Skim Milk ◽  
Survey Method ◽  
Bacillus Sp ◽  
Bacterial Isolates ◽  
Enhalus Acoroides ◽  
Isolation And Characterization ◽  
Protease Enzyme ◽  
Proteolytic Bacteria

AbstrakLamun adalah tumbuhan sejati yang hidup di perairan pantai yang kurang dimanfaatkan dalam bidang perikanan, selain sebagai bioindikator kualitas air laut. Beberapa mikroorganisme yang berasosiasi dengan lamun Enhalus acoroides antara lain benthos, kapang, bakteri dan plankton. Bakteri proteolitik merupakan bakteri yang mampu menghasilkan enzim protease. Enzim perotese merupakan enzim yang paling banyak digunakan dalam kehidupan. Bakteri merupakan sumber enzim yang paling banyak digunakan dibandingkan dengan tanaman dan hewan. Tujuan penelitian ini adalah untuk mengetahui jenis isolat bakteri proteolitik yang berasosiasi dengan lamun Enhalus acoroides di Taman Nasional Baluran, Situbondo. Penelitian ini menggunakan metode survei dengan dianalisis secara deskriptif dengan bantuan tabel dan gambar. Hasil yang peroleh menunjukkan bahwa terdapat 12 isolat bakteri yang berasosisasi dengan lamun Enhalus acoroides. Terdapat empat isolat yang tergolong sebagai bakteri proteolitik karena mampu mendagradasi kasein dalam media TSA + 2% NaCL yang ditambah 1 % susu skim, tampak dari pembentukan zona bening. Pengamatan morfologi koloni dan sel serta pengujian biokimia dari keempat isolat (EA-1, EA-2, EA-9 dan EA-10) terdapat kesamaan karakteristik dengan empat genus bakteri berturut-turut yaitu Staphylococcus sp., Plesiomonas shigeloides, Bacillus sp., Pseudomonas sp.AbstractSeagrass is a true living plants underutilized coastal waters in the field of fisheries, as well as bio-indicators of the quality of sea water. Some microorganisms associated with seagrass Enhalus acoroides among others benthos, fungi, bacteria and plankton. Proteolytic bacteria are bacteria that are capable of producing the enzyme protease. Protease enzyme is an enzyme that is most widely used in life. Bacteria are a source of enzymes that are most widely used compared to plants and animals. The purpose of this study was to determine the type of proteolytic bacterial isolates associated with seagrass Enhalus acoroides in Baluran National Park, Situbondo. This study used survey method with descriptive analysis with tables and figures. The results obtained that there are 12 bacterial isolates associated with seagrass Enhalus acoroides. There are four isolates were classified as proteolytic bacteria because it can degrade casein in TSA media + 2 % NaCL plus 1% skim milk which is evidenced by the formation of clear zones. Observations colony morphology and cells, as well as testing of Biochemistry of the four isolates (EA-1, EA-2, EA-9 and EA-10), were obtained, with similar characteristics to the four genera of bacteria in a row as follows Staphylococcus sp., Plesiomonas shigeloides, Bacillus sp., Pseudomonas sp. 

scholarly journals PROTEOLITIC BACTERIA CULTURE OF ISOLATION RESULTS IN ORGAN LIVING OF FISHING GROUPER CULTURE RESULTS

2020 ◽  
Vol 7 (11) ◽  
pp. 266-275
Author(s):  
Kasprijo ◽  
Manarni
Keyword(s):  
Sea Water ◽  
Anterior Part ◽  
Skim Milk ◽  
Sampling Technique ◽  
Plate Count ◽  
Clear Zone ◽  
Bacterial Colony ◽  
Proteolytic Bacteria ◽  
Observation Method ◽  
Bacteria Culture

The grouper is a sea water fish that is often cultivated. But in digesting feeds is often not optimal. To improve feed efficiency, one effort that can be done is to know the activity of proteolytic bacteria in milkfish digestion. The purpose of this study was to determine the presence and activity of proteolytic bacteria. The method used is the observation method with a purposive sampling technique. The abundance of bacteria in the digestive tract is calculated by the TPC (Total Plate Count) method. Based on the research results, it is known that there are proteolytic bacteria in the digestion group which are indicated by the presence of a clear zone around the bacterial colony that is grown on specific media (skim milk). There is also the activity of proteolytic bacteria in the digestion grouper by measuring the hydrolyzed clear zone with an average in the anterior part of the intestine 2.3 mm, middle 2.6 mm, posterior 2.65 mm. The results of this study indicate that the bacteria bacillus sp with sorbitol media, molasses and nutrient broth with a storage time of 40 days at 4 0C did not experience a decrease in bacterial density that was significantly different.

Isolation, Characterization and Application of Protease Enzyme from Marine Bacteria

2021 ◽  
pp. 4236-4240
Author(s):  
Sneha S ◽  
Mrunal Palsokar ◽  
Vemula Sai Jahnavi ◽  
Anwesha Sarkar ◽  
K. V. Bhaskara Rao
Keyword(s):  
Marine Bacteria ◽  
Peptide Bond ◽  
Mangrove Sediment ◽  
Bacillus Sp ◽  
Bacterial Isolates ◽  
Sediment Samples ◽  
Protease Enzyme ◽  
Stain Removal ◽  
Marine Sediment Sample ◽  
Protease Assay

Protease constitutes the major group of catalytic enzymes which is involved in hydrolyzing peptide bond of proteins. Marine sediment sample were collected and protease producing bacterial isolates were identified by using casein as a substrate. The organisms were characterized by biochemical test and identified as Bacillus sp. In order to check for the production of protease enzyme, quantitative protease assay and Lowry’s method of protein estimation was carried out. The crude extract of protease was subjected for blood stain removal activity and the enzyme proved to be efficient which removed the stain in 15 min. The purpose of the current study is to isolate, identify, characterize and to carry out applications of protease enzyme from marine bacteria isolated from mangrove sediment samples.

scholarly journals Isolation and characterization of psychrotrophic proteolytic bacteria from landfill site under temperate climatic conditions of Kashmir Himalaya

2019 ◽  
Author(s):  
Basharat Hamid ◽  
Arshid Jehangir ◽  
Zahoor Ahmad Baba ◽  
Muneer Ahmad Wani ◽  
Imran Khan
Keyword(s):  
Skim Milk ◽  
Industrial Applications ◽  
Economic Benefits ◽  
Climatic Conditions ◽  
Landfill Site ◽  
Climatic Regions ◽  
Isolation And Characterization ◽  
Cold Active ◽  
Proteolytic Bacteria

The temperate climatic regions face the problem of waste accumulation due to lower environmental temperatures. However, these regions harbor cold active microbes viz. psychrotrophic proteolytic bacteria that play an important role in the degradation of protenaceous materials of the waste stream. Hence in the present study psychrotrophic proteolytic bacteria were isolated from waste samples collected from landfill site by using random sampling method under environmental temperature of 10oC. By using serial dilution and spread plate technique a total of 8 morphologically different psychrotrophic proteolytic bacteria were isolated on skim milk agar media at pH of 7.0 and temperature of 10°C after 48hours. Under in-vitro conditions all the isolates produced significant quantities of protease over the control and diameters of hydrolysis zones ranged between 2 to 18 mm at temperature range of 5 to 20oC and after 72 hours. The corresponding quantitative protease activities of the isolates was significant that ranged between 0.5 to 2.25 U/ml and the isolate PB2 was most efficient with highest protease activity of 2.25U/ml at 20oC. Based on 16SrRNA analysis the isolate was identified as Pseudomonas florescence with 96% similarity. It was concluded that the isolates can grow in wide ranges of temperature and could be used for enhanced decomposition of organic wastes during lower temperature conditions in cold regions. Further the isolates could have industrial applications due to the production of cold active proteases that would help economic benefits through energy conservation.

scholarly journals Isolation and characterization of psychrotrophic proteolytic bacteria from landfill site under temperate climatic conditions of Kashmir Himalaya

2019 ◽  
Author(s):  
Basharat Hamid ◽  
Arshid Jehangir ◽  
Zahoor Ahmad Baba ◽  
Muneer Ahmad Wani ◽  
Imran Khan
Keyword(s):  
Skim Milk ◽  
Industrial Applications ◽  
Economic Benefits ◽  
Climatic Conditions ◽  
Landfill Site ◽  
Climatic Regions ◽  
Isolation And Characterization ◽  
Cold Active ◽  
Proteolytic Bacteria

The temperate climatic regions face the problem of waste accumulation due to lower environmental temperatures. However, these regions harbor cold active microbes viz. psychrotrophic proteolytic bacteria that play an important role in the degradation of protenaceous materials of the waste stream. Hence in the present study psychrotrophic proteolytic bacteria were isolated from waste samples collected from landfill site by using random sampling method under environmental temperature of 10oC. By using serial dilution and spread plate technique a total of 8 morphologically different psychrotrophic proteolytic bacteria were isolated on skim milk agar media at pH of 7.0 and temperature of 10°C after 48hours. Under in-vitro conditions all the isolates produced significant quantities of protease over the control and diameters of hydrolysis zones ranged between 2 to 18 mm at temperature range of 5 to 20oC and after 72 hours. The corresponding quantitative protease activities of the isolates was significant that ranged between 0.5 to 2.25 U/ml and the isolate PB2 was most efficient with highest protease activity of 2.25U/ml at 20oC. Based on 16SrRNA analysis the isolate was identified as Pseudomonas florescence with 96% similarity. It was concluded that the isolates can grow in wide ranges of temperature and could be used for enhanced decomposition of organic wastes during lower temperature conditions in cold regions. Further the isolates could have industrial applications due to the production of cold active proteases that would help economic benefits through energy conservation.

Isolation and characterization of novel and efficient protease producing bacteria from drinking water resources

2019 ◽  
Vol 20 (1) ◽  
pp. 157-164
Author(s):  
S. Jeevan Chandra ◽  
A. Shiva Shanker ◽  
Pavan Kumar Pindi
Keyword(s):  
Industrial Applications ◽  
Protease Production ◽  
Bacillus Sp ◽  
Isolation And Characterization ◽  
Protease Enzyme ◽  
Drinking Water Resources ◽  
Potential Alternative ◽  
Extracellular Protease Activity ◽  
The Stability ◽  
Gene Bank Accession

Abstract An attempt has been made to explore the stability of protease enzyme (isolated from Bacillus sp.) by statistical method. More than 100 isolates were screened for extracellular protease activity derived from various potable water samples of Mahabubnagar district, Telangana State, India. The activity of protease is found to be varying from sample to sample, the highest being reported by the isolate from water sample of Kalwakurthy mandal, Mahabubnagar district and therefore was selected for further studies. The 16S rRNA (ribosomal ribonucleic acid) gene sequence of the isolate showed closest similarity with Bacillus sp. and the sequence was submitted to National Center for Biotechnology Information (NCBI) gene bank (accession number GU566359) and the culture was deposited in three international culture deposition centers (KCTC-13725: MTCC-10465: JCM-16713). The present study revealed that, this Bacillus sp. showed a greater amount of protease production with the inherent characters of thermo, alkali and oxidant stability which makes it a potential alternative protease producing strain in various industrial applications.

scholarly journals Isolation and characterization of psychrotrophic proteolytic bacteria from landfill site under temperate climatic conditions of Kashmir Himalaya

2018 ◽  
Author(s):  
Basharat Hamid ◽  
Arshid Jehangir ◽  
Zahoor Ahmad Baba ◽  
Muneer Ahmad Wani ◽  
Imran Khan
Keyword(s):  
Skim Milk ◽  
Industrial Applications ◽  
Economic Benefits ◽  
Climatic Conditions ◽  
Landfill Site ◽  
Climatic Regions ◽  
Isolation And Characterization ◽  
Cold Active ◽  
Proteolytic Bacteria

The temperate climatic regions face the problem of waste accumulation due to lower environmental temperatures. However, these regions harbor cold active microbes viz. psychrotrophic proteolytic bacteria that play an important role in the degradation of protenaceous materials of the waste stream. Hence in the present study psychrotrophic proteolytic bacteria were isolated from waste samples collected from landfill site by using random sampling method under environmental temperature of 10oC. By using serial dilution and spread plate technique a total of 8 morphologically different psychrotrophic proteolytic bacteria were isolated on skim milk agar media at pH of 7.0 and temperature of 10°C after 48hours. Under in-vitro conditions all the isolates produced significant quantities of protease over the control and diameters of hydrolysis zones ranged between 2 to 18 mm at temperature range of 5 to 20oC and after 72 hours. The corresponding quantitative protease activities of the isolates was significant that ranged between 0.5 to 2.25 U/ml and the isolate PB2 was most efficient with highest protease activity of 2.25U/ml at 20oC. Based on 16SrRNA analysis the isolate was identified as Pseudomonas florescence with 96% similarity. It was concluded that the isolates can grow in wide ranges of temperature and could be used for enhanced decomposition of organic wastes during lower temperature conditions in cold regions. Further the isolates could have industrial applications due to the production of cold active proteases that would help economic benefits through energy conservation.

scholarly journals Eksplorasi Mikroba Penghasil Enzim-enzim Hidrolitik Di Kawasan Taman Nasional Lore Lindu Sulawesi Tengah

2016 ◽  
Vol 18 (2) ◽  
pp. 14
Author(s):  
Rahmiati Rahmiati ◽  
Sri Pujianto ◽  
Endang Kusdiyantini
Keyword(s):  
National Park ◽  
Bacterial Isolate ◽  
Hydrolytic Enzyme ◽  
Microbiology Laboratory ◽  
Bacterial Isolates ◽  
Lipase Enzyme ◽  
Protease Enzyme ◽  
Key Word ◽  
Enzyme Isolation ◽  
Lore Lindu National Park

Lore Lindu National Park (TNLL) is an area that  flora, fauna and microbes, the diversity of microbes producing hydrolytic enzyme. explore the hydrolytic enzyme producing microbes in Indonesia. This study was aimed to obtain bacterial isolates were able to produce hydrolytic enzyme and characteristics. Isolation in the microbiology laboratory. Isolation  by a spread plate. Isolates in the selection hydrolytic enzyme producing selective media. Measurement of the activity of the enzyme with hydrolytic index. The results  were thirteen isolates clearing zone test , 2 protease enzyme bacterial isolates, 1 lipase enzyme bacterial isolates, 6 amylase enzyme bacterial isolates, while 4  cellulase enzyme bacterial isolates. Examination of Amylase enzyme activity was done using DNS method. L10T3 showed that the bacterial isolate optimum activity at pH 7 and at a temperature of 300C with an activity of 0.040 U / mL and 0.029 U / mL. Key word: Lore Lindu National Park, hydrolytic enzyme. isolation of bacteria

scholarly journals Distribusi Bakteri Penghasil Enzim Ekstraseluler Pada Saluran Pencernaan Lobster Mutiara (Panulirus ornatus)

2019 ◽  
Vol 5 (2) ◽  
pp. 71
Author(s):  
Fatur Rahman ◽  
Ismiati Ismiati ◽  
Arbai Nurhasanah
Keyword(s):  
Enzyme Activity ◽  
Digestive Tract ◽  
Extracellular Enzymes ◽  
Extracellular Enzyme ◽  
Extracellular Enzyme Activity ◽  
Bacterial Isolates ◽  
Clear Zone ◽  
Zone Diameter ◽  
Protease Enzyme ◽  
Proteolytic Bacteria

The activity of the digestive function of animals is influenced by the secretion of extracellular enzymes from bacteria in the digestive tract. This study aims to evaluate the distribution of bacteria producing protease enzyme, amylase and lipase from the digestive tract of pearl lobster, Panulirus ornatus. Bacterial isolates that have extracellular enzyme activity are based on their ability to form clear zones in the test media. The results showed that of 51 bacterial isolates from the digestive tract of P. ornatus, proteolytic bacteria were 27.45%, amylolytic bacteria were 23.53% and lipolytic bacteria were 21.77%. Based on bacterial dominance in the gastrointestinal segment, namely the cardiac, piloric and intestinal sections, it was dominated by amylolytic bacteria at 33.33%, proteolytic at 37.50% and lipolytic at 29.41%. The activity of proteolytic, amylolytic and lipolytic bacteria based on the highest clear zone diameter was achieved respectively by SP5 isolates of 12 mm, SK10 isolates of 21 mm and SU15 isolates of 20 mm. The three bacterial isolates were potential as probiotic aquacultur candidates

Cytotoxic Effect of Pseudomonas aurogenosa Protease on Cancer Cells

2019 ◽  
Vol 10 (03) ◽  
Author(s):  
Ghanyia J. Shanyoor ◽  
Fatima R. Abdul ◽  
Nehad A. Taher ◽  
Ihsan A. Raheem
Keyword(s):  
Cell Line ◽  
Normal Cell ◽  
Cytotoxic Effect ◽  
Human Cancer ◽  
Skim Milk ◽  
Exchange Chromatography ◽  
Protease Production ◽  
Normal Cell Line ◽  
Protease Enzyme ◽  
Embryonic Fibroblast

About (20) Pseudomonas rogenosa isolate were experienced for their ability of protease production by calculating the diameter of lysis area after developing on skim milk agar medium (qualitatively ) and the results exhibited that only isolate no (5), was higher isolate for protease making of (26mm) of lysis area. Then, the protein concentration also identified by Bradford method and it was found of 0.16 mg/ ml , then purification was done by using an ion- exchange chromatography with DEAE sephadex G- 100 column and the results showed the presence of 1 peak of enzyme with 50 Kd of molecular weight 2 peaks of other proteins . we tried to investigate the invitro Cytotoxic effect of purified enzyme against two human cancer lines, HeP2 (Human larynx epidermed carcinoma ) , RD ( Rabdo- Sarcoma ) , and one normal cell line Ref ( Rat embryonic fibroblast ) . The cancer and normal cells were treated with different concentrations of protease enzyme ranging from ( 0.05, 0.1, 0.2, 0.4,0.8and 0.16 mg/ml) then incubated for additional 48h at 37C0 and the results showed highest toxicity ( 80.28%) of protease enzyme on RD , moderate cytotoxicity (45.52%) on Hep andslight toxicity ( 37.12% ) on normal cell line (Ref) in a concentration (0.8mg/ml).

Constraints in Adoption ofModern Farm Machines by Tribal Farmers in Ramgarh District of Jharkhand

2019 ◽  
Vol 6 (03) ◽  
Author(s):  
PK SUNDARAM ◽  
BIKASH SARKAR ◽  
UJJWAL KUMAR ◽  
AP ANURAG ◽  
DK RAGHAV ◽  
...  
Keyword(s):  
Cell Line ◽  
Normal Cell ◽  
Human Cancer ◽  
Skim Milk ◽  
Exchange Chromatography ◽  
Protease Production ◽  
Normal Cell Line ◽  
Protease Enzyme ◽  
Tribal Farmers ◽  
Embryonic Fibroblast

About (20) Pseudomonas rogenosa isolate were experienced for their ability of protease production by calculating the diameter of lysis area after developing on skim milk agar medium (qualitatively ) andamp; the results exhibited that only isolate no (5), was higher isolate for protease making of (26mm) of lysis area. Then, the protein concentration also identified by Bradford method andamp; it was found of 0.16 mg/ ml , then purification was done by using an ion- exchange chromatography with DEAE sephadex G- 100 column andamp; the results showed the presence of 1 peak of enzyme with 50 Kd of molecular weight 2 peaks of other proteins . we tried to investigate the invitro Cytotoxic effect of purified enzyme against two human cancer lines, HeP2 (Human larynx epidermed carcinoma ) , RD ( Rabdo- Sarcoma ) , andamp; one normal cell line Ref ( Rat embryonic fibroblast ). The cancer andamp; normal cells were treated with different concentrations of protease enzyme ranging from ( 0.05, 0.1, 0.2, 0.4,0.8andamp; 0.16 mg/ml) then incubated for additional 48h at 37C 0 andamp; the results showed highest toxicity ( 80.28%) of protease enzyme on RD , moderate cytotoxicity (45.52%) on Hep andamp;slight toxicity ( 37.12% )on normal cell line (Ref) in a concentration (0.8mg/ml).

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