Anti-inflammatory and antidiabetic activity of ethanolic extracts of Sterculia villosa barks on Albino Wistar rats

Background/Objective: Euphorbia hirta, Citrus aurantifolia and Heterotis rotundifolia are commonly used in Benin in the treatment of infectious diseases. The aim of this study is to evaluate the anti-inflammatory, analgesic and antipyretic properties of ethanolic extracts of these plants. Materials and Methods: The study was carried out on 30 wistar rats placed in 6 work lots. A positive control lot having received diclofenac and a negative control lot having received physiological water were used. The ethanolic extract of the plants was used at a dose of 200 mg / kg bw. The model of inflammatory edema of the rat paw induced by 2% formalin was used. Analgesic activity was assessed by the pain method induced by 3% acetic acid and the tail immersion method with wistar rats. The antipyretic effect was evaluated on pyrexia induced by brewer's yeast at 20% with wistar rats. Results: After injection of formalin to animals, inflammatory reaction was almost immediate with appearance of classic signs of acute local inflammation (Redness, pain, heat and edema) at the five experimental groups. This inflammatory reaction occurs in two phases. The first phase occurs between 0 and 2 hours after injection of phlogogenic agent and the second phase, initiated after two hours extending to the fifth hour and even beyond. Administration of these extracts prevents edema inflammatory and inhibition percentages of edema vary between 23.67% and 86.76% for the three extracts. These extracts have similar anti-inflammatory activity (p> 0.05) to that of diclofenac at 50 mg/kg. Analgesic activity show that these extracts inhibit very significantly (p<0.001) chemical pain induced by acetic acid and the highest inhibition percentage is 60.34% (Citrus aurantifolia). This percentage is like to that of acetylsalicylic acid (67.35%) administered at the same dose. Likewise, these extracts attenuate significantly (p <0.05) thermal pain induced by tail immersion of each rat in hot water at 50°C. Indeed, these extracts, reduces very significantly (p <0.001) pyrexia induced by 20% beer yeast suspension in rats and they have similar effect (p> 0.05) to that of acetylsalicylic acid at the fourth hour. Conclusion: These results show that the plants studied have the pharmacological properties evaluated. These results justify the use of these plants in traditional medicine.

Download Full-text

Anti-inflammatory, analgesic and antipyretic properties of ethanolic extracts of three plants of Benin pharmacopoeia: Euphorbia hirta, Citrus aurantifolia and Heterotis rotundifolia

10.21203/rs.2.16006/v1 ◽

2019 ◽

Author(s):

Oladélé Gautier ROKO ◽

Victorien Tamègnon DOUGNON ◽

Jean Robert KLOTOE ◽

Lamine BABA-MOUSSA

Keyword(s):

Acetylsalicylic Acid ◽

Wistar Rats ◽

Hot Water ◽

Citrus Aurantifolia ◽

Euphorbia Hirta ◽

Thermal Pain ◽

Ethanolic Extracts ◽

Tail Immersion ◽

Anti Inflammatory ◽

Rat Paw

Abstract Objective Euphorbia hirta , Citrus aurantifolia and Heterotis rotundifolia are commonly used in Benin in the treatment of infectious diseases. The aim of this study is to evaluate the anti-inflammatory, analgesic and antipyretic properties of ethanolic extracts of these plants. The study was performed on wistar rats with 200 mg/kg b.w.Results Administration of these extracts prevents edema inflammatory induced by 2% formalin in rat paw. Inhibition percentages of edema vary between 23.67% and 86.76% for the three extracts. These extracts have similar anti-inflammatory activity (p> 0.05) to that of diclofenac at 50 mg/kg. Analgesic activity show that these extracts inhibit very significantly (p<0.001) the pain induced by 3% acetic acid and the highest inhibition percentage is 60.34% (Citrus aurantifolia ). This percentage is like to that of acetylsalicylic acid (67.35%) administered at the same dose. Likewise, these extracts attenuate significantly (p <0.05) the thermal pain induced by tail immersion of each rat in hot water at 50°C. Indeed, these extracts, reduces very significantly (p <0.001) pyrexia induced by 20% beer yeast suspension in rats and they have similar effect (p> 0.05) to that of acetylsalicylic acid at the fourth hour. These results prove, some traditional therapeutic indications of these plants.

Download Full-text

Anti-inflammatory effects of ethanolic extracts from Micromeria species

Planta Medica ◽

10.1055/s-0030-1264885 ◽

2010 ◽

Vol 76 (12) ◽

Author(s):

M Bival Tefan ◽

D Jelic ◽

S Vladimir-Kneevic ◽

M Trzun ◽

K Frka Boric ◽

...

Keyword(s):

Ethanolic Extracts ◽

Anti Inflammatory

Download Full-text

3,4,5-Trihydroxybenzoic acid attenuates ligature-induced periodontal disease in Wistar rats.

Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry ◽

10.2174/1871523019666200206094335 ◽

2020 ◽

Vol 19 ◽

Author(s):

Ozkan Karatas ◽

Fikret Gevrek

Keyword(s):

Bone Loss ◽

Gallic Acid ◽

Wistar Rats ◽

Alveolar Bone ◽

Alveolar Bone Loss ◽

Collagenase Activity ◽

Osteoblastic Activity ◽

Cell Counts ◽

Experimental Periodontitis ◽

Anti Inflammatory

Background: 3,4,5-Trihydroxybenzoic acid, which is also known as gallic acid, is an anti-inflammatory agent who could provide beneficial effects in preventing periodontal inflammation. The present study aimed to evaluate the anti-inflammatory effects of gallic acid on experimental periodontitis in Wistar rats. Alveolar bone loss, osteoclastic activity, osteoblastic activity, and collagenase activity were also determined. Methods: 32 Wistar rats were used in the present study. Study groups were created as following: Healthy control (C,n=8) group; periodontitis (P,n=8) group; periodontitis and 30 mg/kg gallic acid administered group (G30,n=8); periodontitis and 60 mg/kg gallic acid administered group (G60,n=8). Experimental periodontitis was created by placing 4-0 silk sutures around the mandibular right first molar tooth. Morphological changes in alveolar bone were determined by stereomicroscopic evaluation. Mandibles were undergone histological evaluation. Matrix metalloproteinase (MMP)-8, tissue inhibitor of MMPs (TIMP)-1, bone morphogenetic protein (BMP)-2 expressions, tartrate-resistant acid phosphatase (TRAP) positive osteoclast cells, osteoblast, and inflammatory cell counts were determined. Results: Highest alveolar bone loss was observed in the periodontitis group. Both doses of gallic acid decreased alveolar bone loss compared to the P group. TRAP-positive osteoclast cell counts were higher in the P group, and gallic acid successfully lowered these counts. Osteoblast cells also increased in gallic acid administered groups. Inflammation in the P group was also higher than those of C, G30, and G60 groups supporting the role of gallic acid in preventing inflammation. 30 and 60 mg/kg doses of gallic acid decreased MMP-8 levels and increased TIMP-1 levels. BMP levels increased in gallic acid administered groups, similar to several osteoblasts. Conclusion: Present results revealed an anti-inflammatory effect of gallic acid, which was indicated by decreased alveolar bone loss and collagenase activity and increased osteoblastic activity.

Download Full-text

Assessment of Cota altissima (L.) J. Gay for phytochemical composition and antioxidant, anti-inflammatory, antidiabetic and antimicrobial activities

Zeitschrift für Naturforschung C ◽

10.1515/znc-2020-0257 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Gamze Göger ◽

Muhammed Allak ◽

Ali Şen ◽

Fatih Göger ◽

Mehmet Tekin ◽

...

Keyword(s):

Fatty Acids ◽

Essential Oil ◽

Antioxidant Activities ◽

Antimicrobial Activities ◽

Antidiabetic Activity ◽

Gram Negative Bacteria ◽

Inhibitory Effects ◽

Dicaffeoylquinic Acid ◽

Anti Inflammatory ◽

Phytochemical Profiles

Abstract Phytochemical profiles of essential oil (EO), fatty acids, and n-hexane (CAH), diethyl ether (CAD), ethyl acetate (CAE) and methanol extracts (CAM) of Cota altissima L. J. Gay (syn. Anthemis altissima L.) were investigated as well as their antioxidant, anti-inflammatory, antidiabetic and antimicrobial activites. The essential oil was characterized by the content of acetophenone (35.8%) and β-caryophyllene (10.3%) by GC-MS/FID. Linoleic and oleic acid were found as main fatty acids. The major constituents of the extracts were found to be 5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, isorhamnetin glucoside, quercetin and quercetin glucoside by LC-MS/MS. Antioxidant activities of the extracts were determined by scavenging of DPPH and ABTS free radicals. Also, the inhibitory effects on lipoxygenase and α-glucosidase enzymes were determined. Antimicrobial activity was evaluated against Gram positive, Gram negative bacteria and yeast pathogens. CAM showed the highest antioxidant activity against DPPH and ABTS radicals with IC50 values of 126.60 and 144.40 μg/mL, respectively. In the anti-inflammatory activity, CAE demonstrated the highest antilipoxygenase activity with an IC50 value of 105.40 μg/mL, whereas, CAD showed the best inhibition of α-glucosidase with an IC50 value of 396.40 μg/mL in the antidiabetic activity. CAH was effective against Staphylococcus aureus at MIC = 312.5 µg/mL. This is the first report on antidiabetic, anti-inflammatory and antimicrobial activities of different extracts of C. altissima.

Download Full-text

Defatted Hydroethanolic Extract of Ammodaucus leucotrichus Cosson and Durieu Seeds: Antidiabetic and Anti-Inflammatory Activities

Applied Sciences ◽

10.3390/app10249147 ◽

2020 ◽

Vol 10 (24) ◽

pp. 9147

Author(s):

Imane Es-Safi ◽

Hamza Mechchate ◽

Amal Amaghnouje ◽

Anna Calarco ◽

Smahane Boukhira ◽

...

Keyword(s):

Wistar Rats ◽

Fasting Blood Glucose ◽

Diabetic Mice ◽

North African ◽

Glucose Levels ◽

The North ◽

Blood Glucose Levels ◽

Anti Inflammatory ◽

Hydroethanolic Extract ◽

Inflammatory Effect

The seeds of Ammodaucus leucotrichus Cosson and Durieu have been used in the North African Sahara as a traditional medicine to treat diabetes. The present study investigates the antidiabetic, antihyperglycemic, and anti-inflammatory properties of the defatted hydroethanolic extract of Ammodaucus leucotrichus (DHEAM). The antidiabetic and the antihyperglycemic studies were assessed on alloxan-induced diabetic with orally administered doses of DHEAM (100 and 200 mg/kg). At the same time, its anti-inflammatory propriety was evaluated by measuring edema development in the Wistar rats paw induced with carrageenan. Treatment of diabetic mice with DHEAM for four weeks managed their high fasting blood glucose levels, improved their overall health, and also revealed an excellent antihyperglycemic activity. Following the anti-inflammatory results, DHEAM exhibited a perfect activity. HPLC results revealed the presence of seven molecules (chlorogenic acid, 3-p-coumaroylquinic acid, gallic acid, ferulic acid, myricetin, quercetin, luteolin). This work indicates that the DHEAM has an important antidiabetic, antihyperglycemic, and anti-inflammatory effect that can be well established as a phytomedicine to treat diabetes.

Download Full-text

The potential effects of Centella asiatica ethanolic extracts as an anti-inflammatory agent through decreasing TNF-α expression in indomethacin-induced gastric ulcer model rats

10.1063/5.0053018 ◽

2021 ◽

Author(s):

Priscilla Maiselina Sriepindonnta ◽

Fatimah Nur Fitriani ◽

Savannah Quila Thirza ◽

Made Dinda Pratiwi ◽

Dwi Evan Prima Putra Noviardi ◽

...

Keyword(s):

Gastric Ulcer ◽

Centella Asiatica ◽

Inflammatory Agent ◽

Tnf Α ◽

Ethanolic Extracts ◽

Ulcer Model ◽

Anti Inflammatory

Download Full-text

41. Anti-Inflammatory Effect of Honey Bee Venom on Wistar Rats Induced Poly Cystic Ovarian Syndrome by Estradiol Valerate

Toxicon ◽

10.1016/j.toxicon.2012.04.042 ◽

2012 ◽

Vol 60 (2) ◽

pp. 115-116

Author(s):

Mohammad Nabiuni ◽

Kazem Parivar ◽

Bahman Zeynali ◽

Azar Sheikholeslami ◽

Latifeh Karimzadeh

Keyword(s):

Honey Bee ◽

Wistar Rats ◽

Bee Venom ◽

Estradiol Valerate ◽

Anti Inflammatory ◽

Ovarian Syndrome ◽

Inflammatory Effect

Download Full-text

Evaluation of analgesic and anti-inflammatory activity of ethanolic extract of Enhydra fluctuans on male wistar rats

Journal of Applied Pharmaceutical Research ◽

10.18231/joapr.2021.9.1.37.42 ◽

2021 ◽

Vol 9 (1) ◽

pp. 1-7

Author(s):

Kishor Kumar Roy ◽

Md. Kabirul Islam Mollah ◽

Md. Masud Reja ◽

Dibyendu Shil ◽

Ranjan Kumar Maji

Keyword(s):

Wistar Rats ◽

Pain Threshold ◽

Ethanolic Extract ◽

Inflammatory Activity ◽

Paw Edema ◽

Hot Plate ◽

Plate Method ◽

Traditional System ◽

Male Wistar Rats ◽

Anti Inflammatory

Enhydra fluctuations an edible semi-aquatic vegetable plant are widely used in the traditional system of medicine. Ethanolic extract of Enhydra fluctuans was screened for analgesic & anti-inflammatory activity. Analgesic activity was evaluated by the hot plate method and anti-inflammatory activity was evaluated by formalin induce paw edema in Wistar rats (male). Ethanolic extract dose of 300 mg/kg reduce formalin induce paw inflammation and also increased the pain threshold in rats evidenced by hot plate method. The experimental results concluded that Enhydra fluctuans have significant analgesic and anti-inflammatory activity may due to flavonoid and phenolic compound content.

Download Full-text