scholarly journals EFFECT OF PHOTOPERIODICITY ON CO2 FIXATION BY Chlorella vulgaris Buitenzorg IN BUBBLE COLUMN PHOTOBIOREACTOR FOR FOOD SUPPLEMENT PRODUCTION

2010 ◽  
Vol 8 (2) ◽  
Author(s):  
Anondho Wijanarko ◽  
Dianursanti . ◽  
Arief Budi Witarto ◽  
Roekmijati Widaningroem Soemantojo
2018 ◽  
Vol 8 (3) ◽  
pp. 87
Author(s):  
D Dianursanti ◽  
Rachma Nuzulliany ◽  
Anondho Wijanarko ◽  
M Nasikin

Recently, Chlorella vulgaris is widely studied by experts for its ability as a food supplement and health.  Cultivation of Chlorella vulgaris can be used as a tool to reduce global warming. Chlorella vulgaris can efficiently reduce CO2 because they can grow quickly and easily adapted into the photobioreactor system engineering. This research uses continuous illumination of 5000 lux. However, this lighting condition has limitations because of the intensity given is always constant while the number of Chlorella vulgaris in culture increased. Therefore, one solution to solve it is by using the entrapment of cell or filtration process. Chlorella vulgaris cultivation process carried out in bubble column photobioreactor medium flowed by air containing 5% CO2 with a flow rate of 15.66 m / hr. The process of Chlorella vulgaris cultivation with filtration technique has successfully increased production of biomass up to 1.03 times compared with continuous lighting without filtration with the same amount of inoculum. The final result of dry weight biomass obtained was 0.00756 g/dm3 with shorter cultivation period, 200 hours.  Keywords: filtration, Chlorella vulgaris., constant lighting intensity, bubble column photobioreactor, CO2 fixation.AbstrakBelakangan ini, Chlorella vulgaris diminati oleh para ahli untuk diteliti karena kemampuannya sebagai penghasil biomassa yang bermanfaat sebagai suplemen makanan dan kesehatan. Dalam proses pembudidayaannya, Chlorella vulgaris ini dapat dimanfaatkan sebagai pereduksi pemanasan global. Chlorella vulgaris dapat dengan efisien mereduksi CO2 karena mereka dapat tumbuh dengan cepat dan mudah diadaptasikan ke dalam rekayasa sistem fotobioreaktor. Pencahayaan yang diberlakukan pada penelitian ini adalah pencahayaan kontinu dengan intensitas 5000 lux. Namun, pencahayaan ini memiliki keterbatasan karena intensitas yang diberikan selalu konstan padahal jumlah Chlorella vulgaris dalam kultur semakin meningkat. Oleh sebab itu, salah satu upaya yang dapat dilakukan untuk mengatasinya adalah dengan menggunakan proses pemerangkapan sel atau  filtrasi. Proses Kultivasi Chlorella vulgaris dilakukan dalam fotobioreaktor kolom gelembung skala menengah yang dialiri oleh udara yang mengandung 5% CO2 dengan laju alir sebesar 15,66 m/jam. Proses filtrasi untuk kultivasi Chlorella vulgaris berhasil meningkatkan produksi biomassanya hingga 1,03 kali lipat dibandingkan dengan pencahayaan kontinu tanpa filtrasi dengan jumlah inokulum yang sama. Hasil akhir produksi biomassa adalah 0,00756 g/dm3 dengan masa kultivasi yang lebih singkat yaitu selama 200 jam.Keywords: filtrasi, Chlorella vulgaris., pencahayaan kontinu, fotobioreaktor kolom gelembung, fiksasi CO2.


2006 ◽  
Vol 8 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Anondho Wijanarko ◽  
Dianursanti ◽  
Heidi ◽  
Roekmijati Widaningroem Soemantojo ◽  
Kazuhisa Ohtaguchi

2012 ◽  
Vol 599 ◽  
pp. 137-140 ◽  
Author(s):  
Shu Wen Li ◽  
Sheng Jun Luo ◽  
Rong Bo Guo

The CO2 sequestration by microalgae is thought to be one of the most sustainable strategies to relieve global warming. To produce 1 ton of microalgal dry biomass, 2 ton of CO2 is required. However, insufficient supply of CO2 will limit microalgal growth, and excessive CO2 both means wasting and inhibits microalgal growth. In the present study, the dissolved CO2 concentration in culture limiting and inhibiting microalgal growth (Chlorella vulgaris) in a bubble column photobioreactor was studied. The experimental results showed that the dissolved CO2 concentration ranging from 107μmol/L to 1500 μmol/L could meet microalgal growth’s need, which provides the guidance for microalgal CO2 biofixation with high efficiency.


2007 ◽  
Vol 7 (1 & 2) ◽  
pp. 89 ◽  
Author(s):  
Anondho Wijanarko ◽  
Dianursanti Antonius Yudi Sendjaya ◽  
Misri Gozan ◽  
Roekmijati Widaningroem Soemantojo ◽  
Arief Budi Witarto ◽  
...  

Micro algae are photolitotrophs that perform oxygenic photosynthesis and capable of accumulating a large amount of CO2, using an inducible CO2 concentrating mechanism (CCM). These characteristics make the micro algae potentially useful for removal and utilization of CO2 emitted from industrial plant. Generally, the usage of photosynthetic microorganism in CO2 fixation and biomass production for the economically viable commodities have been increased and significantly improved as a solution for this problem. Using these facts and previous research results using Anabaena cylindrica IAM M1 and Spirulina platensis IAM M 135, enhancement of CO2 fixation and biomass production by Chlorella vulgaris Buitenzorg with photon flux density alteration along with an increasing of culture biomass during the cellular growth period, was implemented in this research. The photon flux density used in this alteration was the maximum light for Chlorella’s maximum growth rate ( I mmax,opt ). The cultivation of Chlorella vulgaris Buitenzorg in the Benneck basal medium operating conditions: T, 29oC; P, 1.0 atm; UG, 2.4m/h; CO2, 10%; using Philip Halogen Lamp 20W/12V/50Hz as the light source and three bubble column photobioreactors arranged in series order with each having a volume of 0.200dm3. Results had shown that the photon flux density alteration as a whole could increase around 60% the biomass production of Chlorella vulgaris and around 7% the CO2 fixation ability, compared to constant photon flux density outcomes. This experiment also showed that the noncompetitive inhibition of [HCO3-] as carbon source substrate is affected significantly during the cultivation in both of alteration and continuous photon flux density.


Biology ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 643
Author(s):  
Konstantin Chekanov ◽  
Daniil Litvinov ◽  
Tatiana Fedorenko ◽  
Olga Chivkunova ◽  
Elena Lobakova

Carotenoids astaxanthin and β-carotene are widely used natural antioxidants. They are key components of functional food, cosmetics, drugs and animal feeding. They hold leader positions on the world carotenoid market. In current work, we characterize the new strain of the green microalga Bracteacoccus aggregatus BM5/15 and propose the method of its culturing in a bubble-column photobioreactor for simultaneous production of astaxanthin and β-carotene. Culture was monitored by light microscopy and pigment kinetics. Fatty acid profile was evaluated by tandem gas-chromatography–mass spectrometry. Pigments were obtained by the classical two-stage scheme of autotrophic cultivation. At the first, vegetative, stage biomass accumulation occurred. Maximum specific growth rate and culture productivity at this stage were 100–200 mg∙L−1∙day−1, and 0.33 day−1, respectively. At the second, inductive, stage carotenoid synthesis was promoted. Maximal carotenoid fraction in the biomass was 2.2–2.4%. Based on chromatography data, astaxanthin and β-carotene constituted 48 and 13% of total carotenoid mass, respectively. Possible pathways of astaxanthin synthesis are proposed based on carotenoid composition. Collectively, a new strain B. aggregatus BM5/15 is a potential biotechnological source of two natural antioxidants, astaxanthin and β-carotene. The results give the rise for further works on optimization of B. aggregatus cultivation on an industrial scale.


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