Combined Production of Astaxanthin and β-Carotene in a New Strain of the Microalga Bracteacoccus aggregatus BM5/15 (IPPAS C-2045) Cultivated in Photobioreactor

Carotenoids astaxanthin and β-carotene are widely used natural antioxidants. They are key components of functional food, cosmetics, drugs and animal feeding. They hold leader positions on the world carotenoid market. In current work, we characterize the new strain of the green microalga Bracteacoccus aggregatus BM5/15 and propose the method of its culturing in a bubble-column photobioreactor for simultaneous production of astaxanthin and β-carotene. Culture was monitored by light microscopy and pigment kinetics. Fatty acid profile was evaluated by tandem gas-chromatography–mass spectrometry. Pigments were obtained by the classical two-stage scheme of autotrophic cultivation. At the first, vegetative, stage biomass accumulation occurred. Maximum specific growth rate and culture productivity at this stage were 100–200 mg∙L−1∙day−1, and 0.33 day−1, respectively. At the second, inductive, stage carotenoid synthesis was promoted. Maximal carotenoid fraction in the biomass was 2.2–2.4%. Based on chromatography data, astaxanthin and β-carotene constituted 48 and 13% of total carotenoid mass, respectively. Possible pathways of astaxanthin synthesis are proposed based on carotenoid composition. Collectively, a new strain B. aggregatus BM5/15 is a potential biotechnological source of two natural antioxidants, astaxanthin and β-carotene. The results give the rise for further works on optimization of B. aggregatus cultivation on an industrial scale.

Download Full-text

Profiling of carotenoids in six microalgae (Eustigmatophyceae) and assessment of their β-carotene productions in bubble column photobioreactor

Biotechnology Letters ◽

10.1007/s10529-012-0996-2 ◽

2012 ◽

Vol 34 (11) ◽

pp. 2049-2053 ◽

Cited By ~ 21

Author(s):

Zhen Li ◽

Mingzhe Sun ◽

Qiyu Li ◽

Aifen Li ◽

Chengwu Zhang

Keyword(s):

Bubble Column ◽

Bubble Column Photobioreactor ◽

Β Carotene

Download Full-text

Cooptimization of growth parameters in glycerol mediated mixotrophic cultivation of microalgae in a bubble column photobioreactor

10.1021/scimeetings.0c03615 ◽

2020 ◽

Author(s):

Prof. Saikat Chakraborty ◽

Sreyashi Ghosh

Keyword(s):

Bubble Column ◽

Growth Parameters ◽

Mixotrophic Cultivation ◽

Bubble Column Photobioreactor

Download Full-text

High efficiency production of astaxanthin by autotrophic cultivation of Haematococcus pluvialis in a bubble column photobioreactor

Biochemical Engineering Journal ◽

10.1016/j.bej.2007.11.010 ◽

2008 ◽

Vol 39 (3) ◽

pp. 575-580 ◽

Cited By ~ 38

Author(s):

Reza Ranjbar ◽

Ryota Inoue ◽

Hironori Shiraishi ◽

Tomohisa Katsuda ◽

Shigeo Katoh

Keyword(s):

High Efficiency ◽

Haematococcus Pluvialis ◽

Bubble Column ◽

Bubble Column Photobioreactor

Download Full-text

Ducrosia ismaelis Asch. essential oil: chemical composition profile and anticancer, antimicrobial and antioxidant potential assessment

Open Chemistry ◽

10.1515/chem-2020-0019 ◽

2020 ◽

Vol 18 (1) ◽

pp. 175-184 ◽

Cited By ~ 1

Author(s):

Ramzi A. Mothana ◽

Fahd A. Nasr ◽

Jamal M. Khaled ◽

Omar M. Noman ◽

Nael Abutaha ◽

...

Keyword(s):

Gas Chromatography ◽

Essential Oil ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Gas Chromatography Mass Spectrometry ◽

Moderate Activity ◽

Antioxidant Agent ◽

Total Oil ◽

Β Carotene ◽

Mcf 7

AbstractThe essential oil of Ducrosia ismaelis Asch. (Apiaceae) that grows wild in Saudi Arabia was investigated utilizing gas chromatography (GC), and gas chromatography-mass spectrometry. Fifty constituents were characterized, representing 96.1% of the total oil. The D. ismaelis essential oil (DIEO) was distinguished by a high composition of oxygenated monoterpenes (51.6%). Decanal (40.6%), α-pinene (15.1%) and dodecanal (13.7%) were the fundamental components. Additionally, DIEO was evaluated for its cytotoxic, antibacterial, antifungal and antioxidant activities. DIEO revealed a great cytotoxic effectiveness against the tested cancer cell lines with IC50 values between 66.2 and 137.3 μg/mL particularly against MCF-7 cancer cells. Furthermore, the induction of apoptosis against MCF-7 cells has been asserted using staining assay (annexin VFITC and/or propidium iodide (PI) dyes) and flow cytometry technique. The DIEO possessed a strong antimicrobial activity against Gram-positive bacterial and fungal strains with MIC-values between 0.07 and 0.31 mg/ml. The values of MBC or MFC were almost once higher than those of MIC’s. Moreover, the β-carotene-bleaching and DPPH free radical-scavenging tests showed that DIEO had a moderate activity (68%) as an antioxidant agent in decolouring of the β-carotene at 1.0 mg/mL and a moderate radical scavenging for DPPH (66 and 72%) at 0.50 and 1.0 mg/mL.

Download Full-text

Production of microalgae Nannochloropsis oculata biomass in a bubble column photobioreactor with integrated lighting through adjustment of light intensity and air flow rate

10.1063/1.5064326 ◽

2018 ◽

Author(s):

Haris Abdul Aziz ◽

Dianursanti Dianursanti

Keyword(s):

Light Intensity ◽

Flow Rate ◽

Bubble Column ◽

Air Flow ◽

Nannochloropsis Oculata ◽

Air Flow Rate ◽

Bubble Column Photobioreactor

Download Full-text

Cooptimization of growth parameters in glycerol mediated mixotrophic cultivation of microalgae in a bubble column photobioreactor

10.1021/scimeetings.0c01959 ◽

2020 ◽

Author(s):

Prof. Saikat Chakraborty ◽

Sreyashi Ghosh

Keyword(s):

Bubble Column ◽

Growth Parameters ◽

Mixotrophic Cultivation ◽

Bubble Column Photobioreactor

Download Full-text

Pilot Study: Does Contamination with Enniatin B and Beauvericin Affect the Antioxidant Capacity of Cereals Commonly Used in Animal Feeding?

Plants ◽

10.3390/plants10091835 ◽

2021 ◽

Vol 10 (9) ◽

pp. 1835

Author(s):

Valentina Serra ◽

Giancarlo Salvatori ◽

Grazia Pastorelli

Keyword(s):

Antioxidant Capacity ◽

Natural Antioxidants ◽

Before And After ◽

Total Antioxidant ◽

Animal Feeding ◽

Preliminary Study ◽

Carry Over ◽

Scientific Attention ◽

Wheat Flours ◽

Larger Sample

Increasing consumption of cereals has been associated with reduced risk of several chronic diseases, as they contain phytochemicals that combat oxidative stress. Cereal contamination by the “emerging mycotoxins” beauvericin (BEA) and enniatins (ENs) is a worldwide health problem that has not yet received adequate scientific attention. Their presence in feeds represents a risk for animals and a potential risk for humans because of their carry-over to animal-derived products. This preliminary study aimed to investigate if the total antioxidant capacity (TAC) of corn, barley, and wheat flours could be influenced by contamination with increasing levels of BEA and ENN B. The highest TAC value was observed in barley compared with wheat and corn (p < 0.001) before and after contamination. No effect of mycotoxin or mycotoxin level was found, whereas cereal x mycotoxin exhibited a significant effect (p < 0.001), showing a lower TAC value in wheat contaminated by ENN B and in barley contaminated by BEA. In conclusion, barley is confirmed as a source of natural antioxidants with antiradical potentials. Additional studies with a larger sample size are necessary to confirm the obtained results, and investigations of the toxic effects of these emergent mycotoxins on animals and humans should be deepened.

Download Full-text

Evaluation of Metabolites and Antioxidant Activity in Pumpkin Species

Natural Product Communications ◽

10.1177/1934578x20920983 ◽

2020 ◽

Vol 15 (4) ◽

pp. 1934578X2092098 ◽

Cited By ~ 1

Author(s):

Hafiz Muhammad Khalid Abbas ◽

He-Xun Huang ◽

Wen-Jie Huang ◽

Shu-Dan Xue ◽

Shi-Juan Yan ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Antioxidant Activity ◽

High Performance Liquid Chromatography ◽

High Performance ◽

Gas Chromatography Mass Spectrometry ◽

Cucurbita Moschata ◽

Cucurbita Maxima ◽

Total Carotenoids ◽

Β Carotene

Cucurbita species ( Cucurbita moschata and Cucurbita maxima) are rich sources of nutrients, especially carotenoids and carbohydrates. Metabolites in pulps of C. moschata and C. maxima lines were examined by high-performance liquid chromatography and gas chromatography–mass spectrometry. Our results revealed that glucose and sucrose were the dominant sugars in C. maxima and C. moschata, respectively. A correlation was found between Brix percentage and sucrose levels as compared with glucose and fructose. A greater amount of myo-inositol had accumulated in C. moschata lines compared with C. maxima. Conversely, total carotenoids and antioxidant activity were found to be higher in C. maxima lines than in C. moschata. A strong correlation of glucose, fructose, and sucrose with β-carotene and violaxanthin revealed that it will be difficult to develop pumpkin cultivars with both high inositol and carotenoid levels. In conclusion, the composition of carbohydrates and carotenoids was more diverse in C. moschata lines than in C. maxima lines. Our results will contribute to a better understanding of metabolite changes in the fruits of these as well as other pumpkin species.

Download Full-text