6. Electrophoretic Analysis Of Isozymes In Cucurbita And Cucumis And Its Application For Phylogenetic Studies

Nine accessions of Bambara groundnut (Vigna subterranea (L.) Verdc.,syn. Voandzeia subterranea (L.) Thouars ex DC.) obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the nine accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions B, C, E, F, H and I have molecular weights not significantly different from one another (P<0.05) while samples A, D and G showed significantly different values (P>0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the nine accessions of Bambara groundnut (Vigna subterraranea (L.) Verdc.syn

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Chapter 11: General epidemiology of TBE

Tick-borne encephalitis - The Book ◽

10.33442/26613980_11-3 ◽

2020 ◽

Keyword(s):

Eastern Siberia ◽

Genetic Lineages ◽

Phylogenetic Studies ◽

Tick Borne Encephalitis ◽

Natural Foci ◽

Genetic Sequences ◽

Tick Borne Encephalitis Virus ◽

Almost All ◽

Far Eastern ◽

Reservoir Hosts

Tick-borne encephalitis virus (TBEV) exists in natural foci, which are areas where TBEV is circulating among its vectors (ticks of different species and genera) and reservoir hosts (usually rodents and small mammals). Based on phylogenetic studies, four TBEV subtypes (Far-Eastern, Siberian, European, Baikalian) and two putative subtypes (Himalayan and “178-79” group) are known. Within each subtype, some genetic lineages are described. The European subtype (TBEV-EU) (formerly known also as the “Western subtype”) of TBEV is prevalent in Europe, but it was also isolated in Western and Eastern Siberia in Russia and South Korea. The Far-Eastern subtype (TBEV-FE) was preferably found in the territory of the far-eastern part of Eurasia, but some strains were isolated in other regions of Eurasia. The Siberian (TBEV-SIB) subtype is the most common and has been found in almost all TBEV habitat areas. The Baikalian subtype is prevalent around Lake Baikal and was isolated several times from ticks and rodents. In addition to the four TBEV subtypes, one single isolate of TBEV (178-79) and two genetic sequences (Himalayan) supposed to be new TBEV subtypes were described in Eastern Siberia and China. The data on TBEV seroprevalence in humans and animals can serve as an indication for the presence or absence of TBEV in studied area.

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Comparative Study of Highly Pathogenic Avian Infl uenza Strains Isolated in Ukraine in 2005 and 2008

Agricultural science and practice ◽

10.15407/agrisp1.01.068 ◽

2014 ◽

Vol 1 (1) ◽

pp. 68-71

Author(s):

A. Gerilovych ◽

B. Stegniy ◽

A. Stegniy ◽

M. Stegniy ◽

K. Smietanka ◽

...

Keyword(s):

Western Europe ◽

Rna Extraction ◽

Experimental Studies ◽

Molecular Characteristics ◽

Eastern European ◽

Highly Pathogenic ◽

Genetic Lineages ◽

Phylogenetic Studies ◽

Polymerase Chain ◽

Pathogenic H5n1

Objective. To research the molecular characteristics of two HPAI strains – A/Ch/Syvash/02/05/H5N1 and A/Ch/Krasnogvardeysk/58/08/H5N1, which were identifi ed as representatives of the highly pathogenic H5N1 viruses. Methods. RNA extraction, real-time polymerase chain reaction (PCR). Results. The phylogenetic studies revealed that the above mentioned strains belong to two various genetic lineages originated from the Eastern European strains isolated in 2005, but differ from the viruses introduced to the Central and Western Europe in 2005/2006, and also the lineages consisting of H5N1 viruses isolated in the Europe and Middle East in late 2007. Conclusions. Relying on experimental studies, it can be concluded that the strains of A/Ch/Syvash/02/05/H5N1 and A/Ch/Krasnogvardeysk/58/08/H5N1 are highly pathogenic.

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Transfer of the Kobresia taxa of the flora of Russia and adjacent countries to the genus Carex (Cyperaceae)

Novitates Systematicae Plantarum Vascularium ◽

10.31111/novitates/2020.51.125 ◽

2020 ◽

pp. 125-129

Author(s):

D. G. Melnikov ◽

L. I. Krupkina

Keyword(s):

International Collaboration ◽

Morphological Characters ◽

Published Data ◽

Phylogenetic Studies ◽

Molecular Phylogenetic ◽

New Nomenclatural Combinations

Based on the published data of molecular phylogenetic studies of the tribe Cariceae Dumort. genera (Cyperaceae), obtained by an international collaboration (The Global Carex Group, 2016; et al.), and morphological characters of the genera (Kukkonen, 1990; and others), new nomenclatural combinations and replacement names in the genus Carex L. are published for 11 species, one subspecies and two sections previously included in the genus Kobresia Willd.

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Phylogenetic studies of Tribe Cacteae (Cactaceae) with special emphasis on the genus Mammillaria

10.31274/rtd-180813-11575 ◽

2003 ◽

Cited By ~ 1

Author(s):

Charles A. Butterworth

Keyword(s):

Phylogenetic Studies

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Future of electrophoretic technology in clinical laboratory testing—From urinary protein electrophoretic analysis to urinary protein pathological analysis—

Electrophoresis Letters ◽

10.2198/electroph.60.23 ◽

2016 ◽

Vol 60 (1) ◽

pp. 23-26

Author(s):

Ryo Kubota ◽

Shiro Iijima ◽

Kiyoko Shiba

Keyword(s):

Laboratory Testing ◽

Clinical Laboratory ◽

Electrophoretic Analysis ◽

Urinary Protein ◽

Pathological Analysis ◽

Clinical Laboratory Testing

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Systematics and evolution of Lentibulariaceae: III. Utricularia

10.1093/oso/9780198779841.003.0008 ◽

2018 ◽

Cited By ~ 3

Author(s):

Richard W. Jobson ◽

Paulo C. Baleeiro ◽

Cástor Guisande

Keyword(s):

Gene Flow ◽

Species Diversity ◽

Population Level ◽

Oceanic Islands ◽

Vulnerable Species ◽

Phylogenetic Studies ◽

Molecular Phylogenetic

Utricularia is a morphologically and ecologically diverse genus currently comprising more than 230 species divided into three subgenera—Polypompholyx, Utricularia, and Bivalvaria—and 35 sections. The genus is distributed worldwide except on the poles and most oceanic islands. The Neotropics has the highest species diversity, followed by Australia. Compared to its sister genera, Utricularia has undergone greater rates of speciation, which are linked to its extreme morphological flexibility that has resulted in the evolution of habitat-specific forms: terrestrial, rheophytic, aquatic, lithophytic, and epiphytic. Molecular phylogenetic studies have resolved relationships for 44% of the species across 80% of the sections. Scant data are available for phylogeography or population-level processes such as gene flow, hybridization, or pollination. Because nearly 90% of the species are endemics, data are urgently needed to determine how to protect vulnerable species and their habitats.

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