scholarly journals Detection-confirmation-standardization-quantification: a novel method for the quantitative analysis of active components in traditional Chinese medicines

2021 ◽  
Vol 3 ◽  
pp. e10
Author(s):  
Xiaohong Song ◽  
Anyi Zhao ◽  
Yan Liu ◽  
Jintang Cheng ◽  
Sha Chen ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Salvia Miltiorrhiza ◽  
Tanshinone Iia ◽  
Preparative Hplc ◽  
Traditional Chinese Medicines ◽  
Reference Standard ◽  
Active Components ◽  
Chinese Medicines ◽  
Tanshinone I ◽  
Novel Method

Background Quantitative analysis of the active ingredients of Traditional Chinese Medicine is a research tendency. The objective of this study was to build a novel method, namely, Detection-confirmation-standardization-quantification (DCSQ), for the quantitative analysis of active components in traditional Chinese medicines, without individual reference standard. Methods Danshen (the dried root of Radix Salvia miltiorrhiza) was used as the matrix. The “extraction” function in high-performance liquid chromatography-mass (HPLC-MS) instrument was used to find the peaks corresponding to cryptotanshinone, tanshinone I, and tanshinone IIA in the total ion current (TIC) chromatogram of Danshen. The multicomponent reference standard (MCRS) containing the three tanshinones mainly was prepared by preparative HPLC. The contents of them in the resulting MCRS were determined by NMR; moreover, the constituents of the MCRS were confirmed. The MCRS containing known content of the three tanshinones was used as the reference standard for the quantitative analysis of cryptotanshinone, tanshinone I and tanshinone IIA in Danshen Samples by analytical HPLC. Results The optimized HPLC conditions for the quantitative analysis of the active components in Danshen were established, and the assignments of the extracted peaks were confirmed by analyzing the characteristic fragments in their MS/MS product ion spectra and UV spectra. Then, the MCRS containing the three tanshinones were successfully prepared. The results of determination about the contents by NMR showed linearity fitted with high likelihood and calibration curves possessed high linearity. The results of determination on Danshen Samples obtained through DCSQ exhibited minimal deviations, in contrast to those obtained through individual reference standards. Conclusion The establishing DCSQ is independent and convenient for the quantitative analysis of the active components in TCMs by MCRS, without individual reference standard. This method is a great advance in quantitative analysis for complex composition, especially TCMs.

scholarly journals Metabolism of Tanshinone IIA, Cryptotanshinone and Tanshinone I from Radix Salvia Miltiorrhiza in Zebrafish

Molecules ◽  
2012 ◽  
Vol 17 (7) ◽  
pp. 8617-8632 ◽  
Author(s):  
Yingjie Wei ◽  
Ping Li ◽  
Changmei Wang ◽  
Yunru Peng ◽  
Luan Shu ◽  
...  
Keyword(s):  
Salvia Miltiorrhiza ◽  
Tanshinone Iia ◽  
Tanshinone I ◽  
Radix Salvia Miltiorrhiza

Chemical Constituents, Quality Control, and Bioactivity of Epimedii Folium (Yinyanghuo)

2015 ◽  
Vol 43 (05) ◽  
pp. 783-834 ◽  
Author(s):  
Xiao-Jia Chen ◽  
Zheng-Hai Tang ◽  
Xi-Wen Li ◽  
Cai-Xiang Xie ◽  
Jin-Jian Lu ◽  
...  
Keyword(s):  
Quality Control ◽  
Nervous System ◽  
Cardiovascular Diseases ◽  
Bone Formation ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Traditional Chinese Medicines ◽  
Active Components ◽  
Chinese Medicines ◽  
Anticancer Effects

Epimedii Folium (Yinyanghuo in Chinese) is one of the most commonly used traditional Chinese medicines. Its main active components are flavonoids, which exhibit multiple biological activities, such as promotion of bone formation and sexual function, protection of the nervous system, and prevention of cardiovascular diseases. Flavonoids also show anti-inflammatory and anticancer effects. Various effective methods, including genetic and chemical approaches, have been developed for the quality control of Yinyanghuo. In this review, the studies conducted in the last decade about the chemical constituents, quality control, and bioactivity of Yinyanghuo are summarized and discussed.

Enhancement of solubility and dissolution rate of cryptotanshinone, tanshinone I and tanshinone IIA extracted from Salvia miltiorrhiza

2012 ◽  
Vol 35 (8) ◽  
pp. 1457-1464 ◽  
Author(s):  
Hang Yu ◽  
Robhash Kusam Subedi ◽  
Pushp R. Nepal ◽  
Yoon G. Kim ◽  
Hoo-Kyun Choi
Keyword(s):  
Dissolution Rate ◽  
Salvia Miltiorrhiza ◽  
Tanshinone Iia ◽  
Tanshinone I

scholarly journals Application of Traditional Chinese Medicines in Postoperative Abdominal Adhesion

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Fuling Wu ◽  
Wenqin Liu ◽  
Haixing Feng ◽  
Li Long ◽  
Lianbing Hou ◽  
...  
Keyword(s):  
Acupuncture Treatment ◽  
Modern Medicine ◽  
Clinical Applications ◽  
Therapeutic Effects ◽  
Traditional Chinese Medicines ◽  
Chinese Medicinal Herbs ◽  
Active Components ◽  
Chinese Medicines ◽  
Abdominal Adhesion ◽  
New Strategies

Adhesion is a frequent complication after abdominal surgery. Although various methods have been applied to prevent and treat postoperative abdominal adhesion (PAA), few modern drugs designed for clinical applications have reached the expected preventive or therapeutic effect so far. There is an imperative to develop some new strategies for the treatment of PAA. Traditional Chinese medicine (TCM) has been widely practiced for thousands of years and played an indispensable role in the prevention and treatment of diseases. Modern medicine researchers have accepted the therapeutic effects of many active components derived from Chinese medicinal herbs. The review stresses the most commonly used TCM treatment, including Chinese medicinal herbals and monomers, TCM formulas, and acupuncture treatment.

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