Differential regulation of antioxidant enzymes in Frankliniella occidentalis (Thysanoptera: Thripidae) exposed to thermal stress

Frankliniella occidentalis is an invasive insect pest that incites damage to ornamental and agronomic crops on a global scale. In this study, the effects of temperature on gene expression and enzyme activity were studied for superoxide dismutase (SOD), peroxidase (POD), and glutathione-S-transferase (GST) in F. occidentalis. SOD, POD and GST enzyme activity increased significantly at 35–37 °C but declined as the temperature increased to 41 °C. In a time course study at 35 °C, SOD, POD and GST activities were significantly elevated at 0.5, 1 and 2 h in comparison to the control at 26 °C. Expression patterns were evaluated for the three antioxidant genes under high and low temperature stress. In a time course study at –4 °C, SOD, POD and GST expression peaked at 1 h and declined at 2 h of exposure. In contrast, when transcription was monitored at 35 °C, expression was lowest at 1 h and increased at 2 h. The results provide data that will be useful in deciphering the role of antioxidant enzymes in the adaptation of F. occidentalis to climate change.

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Alterations in antioxidant enzymes and malondialdehyde content diabetic heart: A time course study

Clinical Biochemistry ◽

10.1016/0009-9120(94)90076-0 ◽

1994 ◽

Vol 27 (3) ◽

pp. 204 ◽

Cited By ~ 2

Author(s):

R. Kakkar ◽

J. Kalra ◽

S.V. Mantha ◽

K. Prasad

Keyword(s):

Antioxidant Enzymes ◽

Time Course ◽

Diabetic Heart ◽

Malondialdehyde Content ◽

Time Course Study

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Time course study of oxidative and nitrosative stress and antioxidant enzymes in K2Cr2O7-induced nephrotoxicity

BMC Nephrology ◽

10.1186/1471-2369-6-4 ◽

2005 ◽

Vol 6 (1) ◽

Cited By ~ 51

Author(s):

José Pedraza-Chaverrí ◽

Diana Barrera ◽

Omar N Medina-Campos ◽

Raymundo C Carvajal ◽

Rogelio Hernández-Pando ◽

...

Keyword(s):

Antioxidant Enzymes ◽

Time Course ◽

Nitrosative Stress ◽

Oxidative And Nitrosative Stress ◽

Time Course Study

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A Time-Course Study of the Effects of Gonadal Hormone Administration on Lipogenie Enzyme Activity in the Liver of the Immature Pullet

Biochemical Society Transactions ◽

10.1042/bst0010769 ◽

1973 ◽

Vol 1 (3) ◽

pp. 769-771 ◽

Cited By ~ 6

Author(s):

J. PEARCE ◽

D. BALNAVE

Keyword(s):

Enzyme Activity ◽

Time Course ◽

Gonadal Hormone ◽

Hormone Administration ◽

Time Course Study

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Mitochondrial proliferation in the permanent vs. temporary cold: enzyme activities and mRNA levels in Antarctic and temperate zoarcid fish

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00111.2003 ◽

2003 ◽

Vol 285 (6) ◽

pp. R1410-R1420 ◽

Cited By ~ 36

Author(s):

M. Lucassen ◽

A. Schmidt ◽

L. G. Eckerle ◽

H.-O. Pörtner

Keyword(s):

Enzyme Activity ◽

Enzyme Activities ◽

Time Course ◽

Citrate Synthase ◽

Expression Patterns ◽

Thermal Adaptation ◽

Lipid Synthesis ◽

Mrna Levels ◽

Transcript Levels ◽

Cold Adapted

Adjustments in mitochondrial properties and capacities are crucial in acclimatization to seasonal cold and in evolutionary cold adaptation of marine ectotherms. Although long-term compensatory increments in aerobic capacity of fish tissues have frequently been described in response to cold, much less is known about transitional phases and gene expression patterns involved. We investigated the time course of adjustment to acute cold in liver of eurythermal eelpout Zoarces viviparus. Whereas citrate synthase (CS) activity rose progressively in liver, cytochrome c oxidase (COX) activity was not altered during cold acclimation. Species-specific RNA probes were used to determine mRNA levels. CS mRNA (nuclear encoded) displayed a delayed, transient increase in response to cold, such that transcript levels did not parallel the change in enzyme activity. The enzyme activities and mRNA levels in the confamilial Antarctic Pachycara brachycephalum indicate cold compensation of CS activity in this cold-adapted species. The ratio of CS and COX activities was elevated in acclimation and adaptation to cold, indicating enhanced citrate synthesis over respiratory chain capacities in cold-adapted liver mitochondria. This may support enhanced lipid synthesis typically found in cold. The ratio of enzyme activity and transcript levels differed largely between Z. viviparus populations from the Baltic and North Seas, indicating the influence of unidentified parameters other than temperature. Transcript levels may not be tightly correlated with enzyme activities during thermal adaptation and thereafter. The time course of the acclimation process indicates that regulation at the translational and posttranslational levels predominates in adjustment to moderate thermal challenges.

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Effects of the Herbicide Pretilachlor and the Safener Fenclorim on Glutathione Content and Glutathione-Dependent Enzyme Activity of Rice

Zeitschrift für Naturforschung C ◽

10.1515/znc-1991-9-1023 ◽

1991 ◽

Vol 46 (9-10) ◽

pp. 861-865 ◽

Cited By ~ 8

Author(s):

Seong Han ◽

Kriton K. Hatzios

Keyword(s):

Enzyme Activity ◽

Time Course ◽

Reduced Glutathione ◽

Oryza Sativa L ◽

Rice Seedlings ◽

Glutathione S Transferase ◽

Simultaneous Application ◽

Related Enzyme ◽

Time Course Study ◽

Gst Activity

Abstract The influence of individual or combined treatments of the chloroacetanilide herbicide pretilachlor and the safener fenclorim on glutathione content and the activity of glutathione reductase (GR, EC 1.6.4.2) and glutathione-S-transferase (GST, EC 2.5.1.18) of rice (Oryza sativa L., var. Lamont) was investigated in a time-course study including 0, 12, 24, 48, and 72 h periods after treatment. Pretilachlor applied alone at 2 μM did not influence the levels of total or reduced glutathione of germinating rice seedlings. Fenclorim applied alone at 20 μM increased significantly the levels of total and reduced glutathione of rice seedlings at 12, 24, and 72 h after treatment. The combination of pretilachlor and fenclorim increased the levels of total and reduced glutathione of rice at all time periods. GR activity extracted from pretilachlor-treated rice seedlings was significantly lower than that extracted from untreated rice seedlings. Pretreatment of rice seedlings with the safener fenclorim increased GR activity at 12 and 24 h, but not at 48 and 72 h after treatment. GR activity extracted from rice seedlings treated with the combination of pretilachlor and fenclorim was significantly higher than that of untreated controls at 12, 24, and 48 h after treatment. Pretreatment of rice seedlings with pretilachlor reduced the activity of GST which catalyzes the conjugation of this herbicide with reduced glutathione at time intervals greater than 24 h. Pretreatment with fenclorim did not have any effect on GST activity of rice seedlings. Pretreatment with the combination of pretilachlor and fenclorim increased GST activity of rice seedlings at the 48 and 72 h periods. These results suggest that a simultaneous application of pretilachlor and fenclorim is critical for the enhancement of glutathione and glutathione-related enzyme activity and the protection of rice from pretilachlor injury.

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Primary cell-culture of the digestive gland of the marine mussel Mytilus edulis : a time-course study of antioxidant- and biotransformation-enzyme activity and ultrastructural changes

Marine Biology ◽

10.1007/s002270050602 ◽

1999 ◽

Vol 135 (1) ◽

pp. 65-75 ◽

Cited By ~ 29

Author(s):

C. Birmelin ◽

R. K. Pipe ◽

P. S. Goldfarb ◽

D. R. Livingstone

Keyword(s):

Cell Culture ◽

Enzyme Activity ◽

Mytilus Edulis ◽

Digestive Gland ◽

Time Course ◽

Primary Cell Culture ◽

Primary Cell ◽

Ultrastructural Changes ◽

Marine Mussel ◽

Time Course Study

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Cloning of a new HSP70 gene from western flowerthrips, Frankliniella occidentalis, and expression patterns during thermal stress

PeerJ ◽

10.7717/peerj.7687 ◽

2019 ◽

Vol 7 ◽

pp. e7687 ◽

Cited By ~ 1

Author(s):

Xiao-xiang Zhang ◽

Jing Qin ◽

Jia-Wen Yuan ◽

Ming-Xing Lu ◽

Yu-Zhou Du

Keyword(s):

Thermal Stress ◽

Thermal Tolerance ◽

Frankliniella Occidentalis ◽

Expression Patterns ◽

Invasive Pest ◽

Hsp70 Gene ◽

Rt Pcr ◽

Gene Encoding ◽

Agronomic Crops ◽

New Gene

Frankliniella occidentalis (Pergande) is an invasive pest that endangers a wide variety of horticultural and agronomic crops. HSP70 is the most important member of the heat shock protein (HSP) family and plays an important role in insect thermal tolerance. In this study, a new gene encoding HSP70 from F. occidentalis, Fohsp706, was selected from the F. occidentalis transcriptome exposed to thermal stress (40 °C) and cloned by RT-PCR and RACE. Further characterization indicated that Fohsp706 localizes to the cytoplasm and does not contain introns. Quantitative real-time reverse transcriptase PCR indicated that Fohsp706 expression was significantly up-regulated by thermal stress; furthermore, there were significant differences in Fohsp706 expression in adults and second instar nymphs after heat stress. Our results indicated that Fohsp706 contributes to thermotolerance in F. occidentalis and provides another example of how this pest adapts to unfavorable environmental conditions.

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