scholarly journals HPLC profiling of selected phenolic acids and flavonoids in Salvia eigii, Salvia hierosolymitana and Salvia viridis growing wild in Jordan and their in vitro antioxidant activity

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9769
Author(s):  
Hala I. Al-Jaber ◽  
Ashok K. Shakya ◽  
Zaha A. Elagbar
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Rosmarinic Acid ◽  
Salvianolic Acid B ◽  
Total Flavonoids ◽  
High Concentration ◽  
Total Flavonoids Content ◽  
Concentration Levels ◽  
In Vitro Antioxidant Activity

Background Salvia eigii., Salvia hierosolymitana and Salvia viridis are native to the Mediterranean region, and are used in traditional medicine for the treatment of many ailments. In the current investigation, the methanolic extracts obtained from the air dried aerial parts of S. eigii, S. hierosolymitana and S. viridis from Jordan were screened for their total phenolics content (TPC), total flavonoids content (TFC) and their in vitro antioxidant activity. Additionally, the presence of four bioactive phenolic acids including gallic acid, caffeic acid, rosmarinic acid and salvianolic acid B and other seven flavonoids including luteolin-7-O-glucoside, apigenin, apigenin-7-O-glucoside, rutin, nariginin, hesperidin and quercetin was determined using Liquid chromatography-Electron Spray Ionization-Tandom Mass Spectrometry (LC-ESI-MS/MS). Methods Antioxidant activity of the obtained three extracts were examined via the DPPH•, ABTS• + radical scavenging methods in addition to Ferrous Ion Chelating (FIC) effect. TFC and TPC of the extracts were measured using the aluminum chloride colorimetric method and the Folin-Ciocalteau method, respectively. The presence and concentration of the selected 11 compounds was further determined through LC-ESI-MS/MS. Results The results indicated that three Salvia species had high total flavonoids content expressed in mg quercetin/g dry extract (S. heirosolymitana: 770.85 ±  5.26; S. eigii: 520.60 ±  6.24, S. viridis: 311.36 ±  4.41). S. heirosolymitana had the highest DPPH• activity (0.184 ±  1.22 × 10−2 mg/ml) and FIC effect (0.354 ±  0.018 mg/ml). S. heirosolymitana had slightly higher ABTS• + scavenging activity than S. eigii (0.176 ±  1.16 × 10−2 mg/ml; 0.183 ±  0.031 mg/ml, respectively). All 11 compounds were detected in the extracts of the three Salvia species. Luteolin-7-O-glucoside was detected in high concentration levels in the three species (1756.73, 21651.36, and 26125.14 mg/kg dry plant; S. eigii, S. hierosolyimitana and S. viridis, respectively), yet rosmarinic acid had the highest contribution to both S. hierosolymitana (27124.93 mg/kg) and S. eigii (15783.33 mg/kg). Notably, S. hierosolymitana and S. viridis contained salvianolic acid B (896.11; 890.9 mg/kg). Conclusions The three Salvia species exhibited good antioxidant activity, especially S. heirosolymitana due to its high TPC, TFC, and the presence of high concentration levels of romarinic acid and other phenolic acids and flavonoids. This is the first phytochemical and antioxidant evaluation of S. eigii, S. hierosolymitana and S. viridis from Jordan. Prior to this investigation, no phytochemical investigation on S. eigii was reported.

scholarly journals Bioactive Content of Rose Hips of Different Wildly Grown Rosa dumalis Genotypes

2016 ◽  
Vol 44 (2) ◽  
pp. 472-476 ◽  
Author(s):  
Sevket ALP ◽  
Sezai ERCISLI ◽  
Tunde JURIKOVA ◽  
Ozlem CAKIR ◽  
Sadiye GOZLEKCI
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Fresh Weight ◽  
Total Carotenoids ◽  
Treatment And Prevention ◽  
Bioactive Content ◽  
Total Flavonoids Content ◽  
Genotype E

A rose hip is the fruit of a rose plant and mostly belongs to Rosa canina and Rosa dumalis. These species are genuine found as wild in natural conditions, while in some countries are cultivated for their hips that are picked and used in a wide variety of preparations. Because they contain a variety of antioxidants, carotenoids, flavonoids, polyphenols, leucoanthocyanins and catechins, rose hips are considered to be a good cancer preventative. The Rosa genus has been the focus of many recent studies due to its potential benefic effects on treatment and prevention of several diseases. However, there are few in vitro studies concerning its composition and antioxidant capacity. The aim of the present study was to determine bioactive content, including ascorbic acid, total phenolics, total flavonoids, total carotenoids and antioxidant activity of Rosa dumalis genotypes growing wild in Erzurum province located eastern Anatolia. The results revealed that fruits of Rosa dumalis different genotypes were rich rich in terms of vitamin C, which ranged from 402 to 511 mg/100 g fresh weight base. The total phenolic content varied from 297 to 403 mg/100 g fresh weight. The genotype 'E-09' had the highest total flavonoids content (229 mg quercetin equivalent/100 g fresh weight) and the lowest value was attributed to genotype 'E-04' with 143 mg/ quercetin equivalent/100 g fresh weight. The antioxidant activity of the genotypes was between 12.9-28.6 μg Trolox/ml samples. The results revealed that there was enough diversity among Rosa dumalis genotypes for bioactive content and promising genotypes, with high bioactive content, were determined, which can become study material for future breeding activities.

scholarly journals Phenolic compounds and in vitro antioxidant activity of six accessions of mashua (Tropaeolum tuberosum R. & P.) from Puno Region, Peru

2021 ◽  
Vol 74 (3) ◽  
pp. 9707-9714
Author(s):  
Haim Behar Montes ◽  
Oscar Reategui ◽  
Danae Marcela Liviac Muñoz ◽  
Jesús Heráclides Arcos Pineda ◽  
Iván Karlos Best Cuba
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Colorimetric Method ◽  
Antioxidant Properties ◽  
Total Content ◽  
Total Flavonoids ◽  
Total Polyphenols ◽  
Tropaeolum Tuberosum ◽  
In Vitro Antioxidant Activity

Mashua (Tropaeolum tuberosum R. & P.) is an Andean crop of high nutritional value and medicinal properties, which presents a great diversity in morphology and color. The aim of the study was to evaluate the content of phenolic compounds and in vitro antioxidant activity of the most economically important mashua accessions in the Puno Region, Peru. Six accessions of mashua (three purplecolored and three yellow-colored) were evaluated. The content of total polyphenols, total flavonoids and identification of phenolic compounds was determined by the Folin-Ciocalteu assay, aluminum chloride colorimetric method and HPLC-DAD, respectively. In vitro antioxidant activity was evaluated using the FRAP and DPPH assays. In general, the purple-colored mashua had a significantly higher content of total polyphenols, total flavonoids, and in vitro antioxidant activity compared to the yellowcolored mashua; being the Tt-23 accession purple-colored (peel/pulp, purple/purple), which presented a significantly higher content of phenolic compounds and in vitro antioxidant activity compared to the other accessions evaluated (P<0.05). Furthermore, a significant correlation was observed between FRAP and DPPH activities with the total content of polyphenols and flavonoids (P<0.01), as well as between FRAP activity and the caffeic acid and rutin levels (P<0.05). These results suggest that purple-colored mashua, particularly the Tt-23 accession (peel/pulp, purple/purple), has better nutraceutical and antioxidant properties due to its higher content of phenolic compounds.

scholarly journals Interactive Effect of Melatonin and UV-C on Phenylpropanoid Metabolite Production and Antioxidant Potential in Callus Cultures of Purple Basil (Ocimum basilicum L. var purpurascens)

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1072 ◽  
Author(s):  
Munazza Nazir ◽  
Muhammad Asad Ullah ◽  
Sadia Mumtaz ◽  
Aisha Siddiquah ◽  
Muzamil Shah ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Rosmarinic Acid ◽  
Interactive Effect ◽  
Hydrogen Atom Transfer ◽  
Ocimum Basilicum ◽  
Antioxidant Potential ◽  
C 50 ◽  
Uv C ◽  
In Vitro Antioxidant Activity

The present study evaluated the interactive effect of melatonin and UV-C on phenylpropanoid metabolites profile and antioxidant potential of Ocimum basilicum L. Callus was treated with varying concentrations of melatonin and UV-C radiations for different time durations, either alone and/or in combination. Individual treatments of both UV-C and melatonin proved to be more effective than combine treatments. Results indicated that UV-C (10 min) exposure increased rosmarinic acid (134.5 mg/g dry weight (DW)), which was 2.3-fold greater than control. Chichoric acid (51.52 mg/g DW) and anthocyanin (cyanide 0.50 mg/g DW) were almost 4.1-fold, while peonidin was found 2.7-fold higher in UV-C (50 min) exposure. In the case of melatonin, 1.0 mg/L concentrations showed maximum rosmarinic acid (79.4 mg/g DW) accumulation; i.e., 1.4-fold more, as compared to the control. However, 2 mg/L melatonin accumulate chichoric acid (39.99 mg/g DW) and anthocyanin (cyanide: 0.45 mg/g DW and peonidin: 0.22 mg/g DW); i.e., 3.2, 3.7 and 2.0-fold increase, as compared to the control, respectively. On the other hand, melatonin-combined treatment (melatonin (Mel) (4 mg/L) + UV-C (20 min)) was proved to be effective in caffeic acid elicitation, which was 1.9-fold greater than the control. Furthermore, antioxidant potential was evaluated by both in vitro (DPPH, ABTS and FRAP assays) and in cellulo methods. Maximum in vitro antioxidant activity (DPPH: 90.6% and ABTS: 1909.5 µM) was observed for UV-C (50 min)-treated cultures. The highest in vitro antioxidant activity measured with the ABTS assay as compared to the FRAP assay, suggesting the main contribution of antioxidants from basil callus extracts acting through a hydrogen atom transfer (HAT) over an electron transfer (ET)-based mechanism. Cellular antioxidant assay was evaluated by production of ROS/RNS species using yeast cell cultures and further confirmed the protective action of the corresponding callus extracts against oxidative stress. Overall, both melatonin and UV-C are here proved to be effective elicitors since a positive correlation between the induced production of phenolic compounds, and in cellulo antioxidant action of basil callus extracts were observed.

scholarly journals Estimation of flavonoids and screening of in vitro antioxidant activity of various extracts of aerial parts of Blepharis maderaspatensis by hydroxyl radical and total antioxidant activity

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1204-1209
Author(s):  
Jambula Dinesh Babu ◽  
Bhuvaneswari Santharam ◽  
Kottai Muthu Arumugam
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Bioactive Compound ◽  
Antioxidant Potential ◽  
Total Flavonoids ◽  
Total Antioxidant Activity ◽  
Aerial Parts ◽  
Total Antioxidant ◽  
Total Flavonoids Content

Antioxidants are substances that hamper or inhibit the oxidation of oxidizable substrates in the cells. The aerial parts of Blepharis maderaspatensis dry powder was extracted with various solvents (PE, EA and methanol) through Soxhlet extractor. The aerial parts of different concentrates (Pet.ether, ethyl acetate and methanol) of Blepharis maderaspatensis was evaluated for its in-vitro antioxidant potential by hydroxyl radical, total antioxidant activity taking ascorbate used as standard for the both methods and total flavonoids content was estimated as equivalent to rutin. The methanolic concentrates of Blepharis maderaspatensis & standard exhibited antioxidant potential possessing IC50 210µg/ml & 62µg/ml (hydroxyl radical) 200µg/ml & 57µg/ml (Total antioxidant activity) respectively. An IC50 value was originate that methanolic concentrates of Blepharis maderaspatensis more efficient in hydroxyl radical, total antioxidant activity compared EA & PE concentrates. The methanolic and EA concentrates of Blepharis maderaspatensis showed the total flavonoids content (11.230±0.22 and 2.858±0.56 mg of rutin equivalent/g of powder) respectively. The difference in scavenging potential of the extracts can be due to variation in the percentage of bioactive compound flavonoids present in methanolic extracts. Invitro antioxidant studies obviously show methanolic concentrates of Blepharis maderaspatensis have better antioxidant activity due to the presence of total flavonoids content. The findings from the study reveal that the antioxidative potentials of methanolic concentrates Blepharis maderaspatensis could serve as free radical inhibitors, possibly acting as natural antioxidants.

Evaluation of the Phenolics and in vitro Antioxidant Activity of Different Botanical Herbals Used for Tea Infusions in Brazil

2019 ◽  
Vol 15 (4) ◽  
pp. 345-352
Author(s):  
Acácio A.F. Zielinski ◽  
Charles W.I. Haminiuk ◽  
Trust Beta
Keyword(s):  
Cluster Analysis ◽  
Antioxidant Activity ◽  
Hierarchical Cluster Analysis ◽  
Hierarchical Cluster ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Tannin Content ◽  
Herbal Infusions ◽  
In Vitro Antioxidant Activity

Background: The consumption of herbal teas has gained much attention due to its healthpromoting benefits, including antioxidant, neuroprotective, antimicrobial, antitumor, and antiinflammatory effects. These biological activities are associated in part to the antioxidant activity of chemical compounds present in teas, especially flavonoids and phenolic acids. Objective: The aim of this study was to evaluate a total of 17 different botanical herbal infusions consumed in Brazil in terms of their phenolic antioxidants. Methods: The analysis performed were total phenolic compounds, total flavonoids, total flavonols, tannin content and in vitro antioxidant activity (DPPH, ABTS, CUPRAC, FRAP, and ORAC assays). Data were processed using univariate, bivariate and multivariate analysis (hierarchical cluster analysis). Results: The use of Hierarchical Cluster Analysis (HCA) suggested an unsupervised classification relationship based on level of functionality of the herbal teas. Higher levels of total phenolics, total flavonoids and antioxidant activity were found in Anemopaegma mirandum while higher values of tannin content and total flavonols were found in Peumus boldus. All antioxidant activity assays showed significant correlations among each other (r > 0.84, p < 0.001), and with total phenolic and flavonoids (r > 0.83, p < 0.001). Using HCA, three clusters were suggested and cluster 1 showed the highest functionality. Conclusion: The herbal infusions evaluated can be a good resource of bioactive compounds to consume and supplementing food products. Nevertheless, future studies should focus on the evaluation of these herbal teas using in vivo systems to understand the mechanisms of action when these different herbal infusions are used as beverages.

In vitro Antioxidant activity and Phytochemical composition of Syringodium isoetifolium

2021 ◽  
pp. 6201-6206
Author(s):  
Kalaivani P. ◽  
Kavitha D. ◽  
Amudha P.
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
High Concentration ◽  
Hydroalcoholic Extract ◽  
Therapeutic Uses ◽  
Syringodium Isoetifolium ◽  
Shallow Estuaries ◽  
In Vitro Antioxidant Activity

Seagrass are the marine flowering plants found mainly in clear, shallow estuaries and coastal waters. In all temperate and trophical region seagrasses grow both internally and subtidally. One such seagrass namely Syringodium isoetifolium has many medicinal properities. This seagrass have most promising pharmacological activities which may include anti-inflammatory, anticancer, antidiarrheal, antihaemorrhoidal activities. This study is focussed on the phytochemical evaluation and in vitro antioxidant activity of aqueous, ethanol and hydroalcoholic extract of Syringodium isoetifolium. The qualitative analysis of Syringodium isoetifolium shows the presence of tannin, saponin, flavonoids, steroids, terpenoids, alkaloids, anthraquinone, polyphenol and coumarin. In all the three extracts only ethanol shows the high concentration of phytocompounds. Emodins, glycoside and anthocyanin were found to be absent in all the three extracts. Quantitative analysis of total phenol, flavonoid, saponin and tannin were found to be 193.10 ±13.52, 106.11 ± 7.42, 52.96 ± 3.64 and 81.30 ± 5.69. Superoxide anion radical, Nitric oxide and Hydroxy radical scavenging assay showed that Syringodium isoetifolium was an excellent scavenger of these radicals. These results are an indication of the potent antioxidant property of the extract and may be responsible for some of the therapeutic uses of Syringodium isoetifolium.

In vitro antioxidant activity and tannin content of Echium italicum L.

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
N Niciforovic ◽  
S Solujic ◽  
V Mihailovic ◽  
D Pavlovic-Muratspahic
Keyword(s):  
Antioxidant Activity ◽  
Tannin Content ◽  
In Vitro Antioxidant Activity
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